Q993M1 (RDRP_GGNNV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: RNA-directed RNA polymerase Short name=RdRp EC=2.7.7.48 Alternative name(s): RNA replicase Short name=Protein A
Organism	Greasy grouper nervous necrosis virus (GGNNV) (Epinephelus tauvina nervous necrosis virus)
Taxonomic identifier	143921 [NCBI]
Taxonomic lineage	Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Nodaviridae › Betanodavirus › unclassified Betanodavirus
Virus host	Epinephelus tauvina (greasy grouper) [TaxID: 203262]

Protein attributes

Sequence length	982 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	RNA-dependent RNA polymerase which replicates the viral genome composed of 2 RNA segments, RNA1 AND RNA2 Potential.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Sequence similarities	Belongs to the nodaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Viral RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 982	982	RNA-directed RNA polymerase		PRO_0000222447
Regions
	Domain	579 – 701	123	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q993M1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: CC61FB47B75CE0DA	FASTA	982	110,395
	10 20 30 40 50 60 MRRFEFALAR MSGAAFCVYT GYRLLTSKWL ADRVEDYRQR IIAEKKQILR DAAMIRTQIQ 70 80 90 100 110 120 REMELVRISV RKGHSHQEAA TERNSATETM LGVVEKCGYE PYVISPSPRE VGYHGSRQFY 130 140 150 160 170 180 SLADFRQDYR RDDITDRHII VMTDVDYYVD MHELIGLGVP ILLYTFQPST VSGEVKDGYF 190 200 210 220 230 240 TITDDSVHYR VAGGKDVRHR IWNYNHDTMY VCSRPRGFWA NLMQILRDIT GVTAICSFLY 250 260 270 280 290 300 TKLGIAPFGD PVTMFTVDQF KMGEHRNIVS IVPFATCRSN LLKISEYGAE LEYMRYQQRN 310 320 330 340 350 360 NIANFNAVTY ISENGPLISL GLEGNFASVQ LPLQDFENIR TAYELSKTNN LSDTVRRSGR 370 380 390 400 410 420 PCKEAAIIHK CLQAECAVVS EVVHKPGDLA RHYQAVGSAY DTDPAEQGKC YAREYAPGPL 430 440 450 460 470 480 TQTAVFPSES RSNELATIDG RIAGPQAKAK SREHITPKMR KVARDFVHHL VPIAGTGRPY 490 500 510 520 530 540 PLTYVEEQQT KPLQRARNDA NRYHDEFTMM VKAFQKKEAY NAPNYPRNIS TVPHTQNVKL 550 560 570 580 590 600 SSYTYAFKAS VLQHVPWYMP THTPAEIADA VQNLAASSTE LVETDYSKFD GTFLRFMREC 610 620 630 640 650 660 VEFAIYKRWV HLDHLPELTT LLANEIQAPA VTRLGIKYDP DCSRLSGSAL TTDGNSIANA 670 680 690 700 710 720 FVSYLAGRMA GMDDDEAWSW IGIVYGDDGL RSGNVSNELL TNTASSLGFD LKIVNRAPRG 730 740 750 760 770 780 SPVTFLSRVY LDPWSSPASV QSPLRTLLKL HTTCDTQSEI DDIGWAKTQA YLVTDSKTPF 790 800 810 820 830 840 IGHWCRAYQR NCTARVVQYA DYADIPFWVK NDDHVGNSWP QSESDDWNDI VANELGVTTA 850 860 870 880 890 900 ELLKHLALLD AYAGPISGLP RLTTSIDLEP KMSVALDGEI QAGPSQNKTS KDGTNPTSDR 910 920 930 940 950 960 SAPRRARAAL PGDDGHARRS RRSDRDPGKR DAHVRDKRPR RSSPPTRPVT PVPTPSSGDR 970 980 GTDGDGLGRA AVRQRQRRRT QV

References

[1]

"Determination of the complete nucleotide sequences of RNA1 and RNA2 from greasy grouper (Epinephelus tauvina) nervous necrosis virus, Singapore strain." Tan C., Huang B., Chang S.F., Ngoh G.H., Munday B., Chen S.C., Kwang J. J. Gen. Virol. 82:647-653(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Singapore.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF319555 mRNA. Translation: AAK21877.1.
RefSeq	NP_689433.1. NC_004137.1.
3D structure databases
ProteinModelPortal	Q993M1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	984290.
Family and domain databases
InterPro	IPR007094. RNA-dir_pol_PSvirus. IPR001788. Tymovirus_RNA-dep_RNA_pol. [Graphical view]
Pfam	PF00978. RdRP_2. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_GGNNV

Accession

Primary (citable) accession number: Q993M1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program