Q99396 (CY42_TRYBB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Receptor-type adenylate cyclase GRESAG 4.2
EC=4.6.1.1

Alternative name(s):
ATP pyrophosphate-lyase
Adenylyl cyclase

Gene names

Name:

GRESAG 4.2

Organism

Trypanosoma brucei brucei

Taxonomic identifier

5702 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma

Protein attributes

Sequence length	572 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Could act as a receptor for a unknown ligand.
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the adenylyl cyclase class-3 family. Contains 1 guanylate cyclase domain.

Ontologies

Keywords
Biological process	cAMP biosynthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Lyase Receptor
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 572	›572	Receptor-type adenylate cyclase GRESAG 4.2		PRO_0000195738
Regions
	Topological domain	‹1 – 225	›225	Extracellular Potential
	Transmembrane	226 – 251	26	Helical; Potential
	Topological domain	252 – 572	321	Cytoplasmic Potential
	Domain	269 – 424	156	Guanylate cyclase
Sites
	Metal binding	274	1	Magnesium By similarity
	Metal binding	317	1	Magnesium By similarity
Amino acid modifications
	Glycosylation	10	1	N-linked (GlcNAc...) Potential
	Glycosylation	77	1	N-linked (GlcNAc...) Potential
	Glycosylation	152	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q99396 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1F3EE304E091AFDD	FASTA	572	63,683
	10 20 30 40 50 60 RKTLLTFGLN LSSSVVLTPG DSVGEHLPKS GITFIIGLVV DDIVVIEEHL RIHTKARVLV 70 80 90 100 110 120 QFSDIALLYN EFVQAFNNSD GAKHLLFATS LPHWADVDTT SETVRRFHEA VPEVEKWTPL 130 140 150 160 170 180 SLLGFATGRL MQRNLQRMDL ATSDLLSGLF FNETFITVDD MQYGAYKDAE GVATAEESLS 190 200 210 220 230 240 NFGATDISVW SMARALRSDE PVLQDPMSPS MVYTVPNGNA LTPAQLDWCG WCRFACADTG 250 260 270 280 290 300 SRLTVFLCCI MRNKRDNDNA PKELADPVTL IFTDIESSTA QWATQPELMP DAVATHHSMV 310 320 330 340 350 360 RSLIENYDCY EVKTVGDSFM IACKSPFAAV QLAQELQLRF LRLDWGTTVF DEFYREFEER 370 380 390 400 410 420 HAEEGDGKYK PPTARLDPEV YRQLWNGLRV RVGIHTGLCD IRYDEVTKGY DYYGQTANTA 430 440 450 460 470 480 ARTESVGNGG QVLMTCETYH SLSTAERSQF DVTPLGGVPL RGVSEPVEVY QLNAVPGRSF 490 500 510 520 530 540 AELRLDRVLD VLDIFGEGTA ASTSDYSSTL AELSETAQAI AVSLQSLMGV FTQAQRQGTL 550 560 570 MPFCERWRVR CPRKVHPRGT TATVRRLSVA LQ

References

[1]	"Differential expression of a family of putative adenylate/guanylate cyclase genes in Trypanosoma brucei." Alexandre S., Paindavione P., Tebabi P., Pays A., Halleux S., Steinert M., Pays E. Mol. Biochem. Parasitol. 43:279-288(1990) [PubMed: 1982555] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: EATRO 1125.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52120 mRNA. Translation: CAA36365.1.
PIR	S14200.
3D structure databases
ProteinModelPortal	Q99396.
SMR	Q99396. Positions 256-487.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001054. A/G_cyclase. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 1 hit.
Pfam	PF00211. Guanylate_cyc. 1 hit. [Graphical view]
SMART	SM00044. CYCc. 1 hit. [Graphical view]
SUPFAM	SSF55073. A/G_cyclase. 1 hit.
PROSITE	PS50125. GUANYLATE_CYCLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CY42_TRYBB

Accession

Primary (citable) accession number: Q99396

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 31, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)