Q99394 (TRS33_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trafficking protein particle complex subunit 33
Short name=TRAPP subunit 33

Alternative name(s):
Transport protein particle 33 kDa subunit

Gene names

Name:	TRS33
Ordered Locus Names:	YOR115C
ORF Names:	O3251, YOR3251C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation. Required for sporulation. Has a role late in meiosis following DNA replication. Ref.6 Ref.7 Ref.8
Subunit structure	Part of the multisubunit TRAPP (transport protein particle) I complex composed of BET3, BET5, TRS20, TRS23, TRS31 and TRS33. Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS85, TRS120 and TRS130. Part of the multisubunit TRAPP (transport protein particle) III complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33 and TRS85. Ref.5 Ref.6 Ref.7 Ref.8
Subcellular location	Golgi apparatus › cis-Golgi network. Endoplasmic reticulum. Preautophagosomal structure Ref.6 Ref.8.
Sequence similarities	Belongs to the TRAPP small subunits family. BET3 subfamily.

Ontologies

Keywords
Biological process	Autophagy ER-Golgi transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from genetic interaction Ref.5. Source: SGD autophagy Inferred from electronic annotation. Source: UniProtKB-KW protein complex assembly Inferred from genetic interaction PubMed 19843283. Source: SGD
Cellular_component	TRAPP complex Inferred from direct assay PubMed 10727015Ref.5. Source: SGD endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell pre-autophagosomal structure Inferred from electronic annotation. Source: UniProtKB-SubCell trans-Golgi network Inferred from direct assay PubMed 19843283. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BET3	P36149	8	EBI-19480,EBI-3567

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Trafficking protein particle complex subunit 33		PRO_0000211589

Sequences

	Sequence		Length	Mass (Da)
	Q99394 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2480F69D7A48D363	FASTA	268	30,749
	10 20 30 40 50 60 MSSTHSNNVG HPQSSPQGPL TEQQRAQQQY QIFENSLPKV SQSVYQMLLN EMVPLAMGIE 70 80 90 100 110 120 RQISGDVISS DSNVTSENGN INNMIKRLKI EEHHTVDIIR SHNLIHELYK ADEEEKEKVL 130 140 150 160 170 180 ARLRNIGFQI GLKLSELLIF SNNPNLKFKE MDLLLIMKFI CRDVWKQIFG KQIDNLKTNH 190 200 210 220 230 240 RGTFYLLDYD YRPIQSFSLE EDAKNEELKM IEPFLEIPVG IIRGVLSSLG YSSEEVICLA 250 260 SFIDRPTDRP KTAFPKGVSF HVQVTMPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and analysis of 51 kb on the right arm of chromosome XV from Saccharomyces cerevisiae reveals 30 open reading frames." Wiemann S., Rechmann S., Benes V., Voss H., Schwager C., Vlcek C., Stegemann J., Zimmermann J., Erfle H., Paces V., Ansorge W. Yeast 12:281-288(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"DNA sequencing and analysis of 130 kb from yeast chromosome XV." Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C., Schwager C., Paces V., Sander C., Ansorge W. Yeast 13:655-672(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion." Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S. EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TRAPP II COMPLEX.
[6]	"TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport." Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S. Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, FUNCTION OF THE TRAPP I COMPLEX, IDENTIFICATION IN THE TRAPP I COMPLEX, SUBCELLULAR LOCATION.
[7]	"Molecular architecture of the TRAPPII complex and implications for vesicle tethering." Yip C.K., Berscheminski J., Walz T. Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
[8]	"Trs85 directs a Ypt1 GEF, TRAPPIII, to the phagophore to promote autophagy." Lynch-Day M.A., Bhandari D., Menon S., Huang J., Cai H., Bartholomew C.R., Brumell J.H., Ferro-Novick S., Klionsky D.J. Proc. Natl. Acad. Sci. U.S.A. 107:7811-7816(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TRAPP III COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE TRAPP III COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X90518 Genomic DNA. Translation: CAA62124.1. X94335 Genomic DNA. Translation: CAA64035.1. Z75023 Genomic DNA. Translation: CAA99313.1. BK006948 Genomic DNA. Translation: DAA10890.1.
PIR	S61003.
RefSeq	NP_014758.3. NM_001183534.3.
3D structure databases
ProteinModelPortal	Q99394.
SMR	Q99394. Positions 113-233.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1712N.
IntAct	Q99394. 6 interactions.
MINT	MINT-387700.
STRING	4932.YOR115C.
Proteomic databases
PaxDb	Q99394.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR115C; YOR115C; YOR115C.
GeneID	854282.
KEGG	sce:YOR115C.
Organism-specific databases
CYGD	YOR115c.
SGD	S000005641. TRS33.
Phylogenomic databases
eggNOG	NOG255558.
GeneTree	ENSGT00390000012948.
HOGENOM	HOG000200502.
OMA	HRGTFYL.
OrthoDB	EOG41RT4C.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33644-MONOMER.
Gene expression databases
Genevestigator	Q99394.
GermOnline	YOR115C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR024096. NO_sig/Golgi_transp_ligand-bd. IPR007194. TRAPP_component. [Graphical view]
Pfam	PF04051. TRAPP. 1 hit. [Graphical view]
SUPFAM	SSF111126. Haem_NO-bd. 1 hit.
ProtoNet	Search...
Other
NextBio	976254.

Entry information

Entry name

TRS33_YEAST

Accession

Primary (citable) accession number: Q99394
Secondary accession number(s): D6W2H4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program