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Q99394

- TRS33_YEAST

UniProt

Q99394 - TRS33_YEAST

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Protein

Trafficking protein particle complex subunit 33

Gene

TRS33

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation. Required for sporulation. Has a role late in meiosis following DNA replication.3 Publications

GO - Biological processi

  1. autophagy Source: UniProtKB-KW
  2. ER to Golgi vesicle-mediated transport Source: SGD
  3. protein complex assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, ER-Golgi transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33644-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Trafficking protein particle complex subunit 33
Short name:
TRAPP subunit 33
Alternative name(s):
Transport protein particle 33 kDa subunit
Gene namesi
Name:TRS33
Ordered Locus Names:YOR115C
ORF Names:O3251, YOR3251C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR115c.
SGDiS000005641. TRS33.

Subcellular locationi

GO - Cellular componenti

  1. cis-Golgi network Source: SGD
  2. endoplasmic reticulum Source: UniProtKB-KW
  3. trans-Golgi network Source: SGD
  4. TRAPPIII protein complex Source: SGD
  5. TRAPPII protein complex Source: SGD
  6. TRAPPI protein complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 268267Trafficking protein particle complex subunit 33PRO_0000211589Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ99394.
PaxDbiQ99394.

Expressioni

Gene expression databases

GenevestigatoriQ99394.

Interactioni

Subunit structurei

Part of the multisubunit TRAPP (transport protein particle) I complex composed of BET3, BET5, TRS20, TRS23, TRS31 and TRS33. Part of the multisubunit TRAPP (transport protein particle) II complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33, TRS65, TRS85, TRS120 and TRS130. Part of the multisubunit TRAPP (transport protein particle) III complex composed of BET3, BET5, TRS20, TRS23, TRS31, TRS33 and TRS85.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BET3P361498EBI-19480,EBI-3567

Protein-protein interaction databases

BioGridi34511. 56 interactions.
DIPiDIP-1712N.
IntActiQ99394. 7 interactions.
MINTiMINT-387700.
STRINGi4932.YOR115C.

Structurei

3D structure databases

ProteinModelPortaliQ99394.
SMRiQ99394. Positions 113-233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG255558.
GeneTreeiENSGT00390000012948.
HOGENOMiHOG000200502.
InParanoidiQ99394.
OMAiDIQATHE.
OrthoDBiEOG7B8SF4.

Family and domain databases

InterProiIPR024096. NO_sig/Golgi_transp_ligand-bd.
IPR007194. TRAPP_component.
[Graphical view]
PfamiPF04051. TRAPP. 1 hit.
[Graphical view]
SUPFAMiSSF111126. SSF111126. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99394-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSTHSNNVG HPQSSPQGPL TEQQRAQQQY QIFENSLPKV SQSVYQMLLN
60 70 80 90 100
EMVPLAMGIE RQISGDVISS DSNVTSENGN INNMIKRLKI EEHHTVDIIR
110 120 130 140 150
SHNLIHELYK ADEEEKEKVL ARLRNIGFQI GLKLSELLIF SNNPNLKFKE
160 170 180 190 200
MDLLLIMKFI CRDVWKQIFG KQIDNLKTNH RGTFYLLDYD YRPIQSFSLE
210 220 230 240 250
EDAKNEELKM IEPFLEIPVG IIRGVLSSLG YSSEEVICLA SFIDRPTDRP
260
KTAFPKGVSF HVQVTMPQ
Length:268
Mass (Da):30,749
Last modified:November 1, 1996 - v1
Checksum:i2480F69D7A48D363
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X90518 Genomic DNA. Translation: CAA62124.1.
X94335 Genomic DNA. Translation: CAA64035.1.
Z75023 Genomic DNA. Translation: CAA99313.1.
BK006948 Genomic DNA. Translation: DAA10890.1.
PIRiS61003.
RefSeqiNP_014758.3. NM_001183534.3.

Genome annotation databases

EnsemblFungiiYOR115C; YOR115C; YOR115C.
GeneIDi854282.
KEGGisce:YOR115C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X90518 Genomic DNA. Translation: CAA62124.1 .
X94335 Genomic DNA. Translation: CAA64035.1 .
Z75023 Genomic DNA. Translation: CAA99313.1 .
BK006948 Genomic DNA. Translation: DAA10890.1 .
PIRi S61003.
RefSeqi NP_014758.3. NM_001183534.3.

3D structure databases

ProteinModelPortali Q99394.
SMRi Q99394. Positions 113-233.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34511. 56 interactions.
DIPi DIP-1712N.
IntActi Q99394. 7 interactions.
MINTi MINT-387700.
STRINGi 4932.YOR115C.

Proteomic databases

MaxQBi Q99394.
PaxDbi Q99394.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR115C ; YOR115C ; YOR115C .
GeneIDi 854282.
KEGGi sce:YOR115C.

Organism-specific databases

CYGDi YOR115c.
SGDi S000005641. TRS33.

Phylogenomic databases

eggNOGi NOG255558.
GeneTreei ENSGT00390000012948.
HOGENOMi HOG000200502.
InParanoidi Q99394.
OMAi DIQATHE.
OrthoDBi EOG7B8SF4.

Enzyme and pathway databases

BioCyci YEAST:G3O-33644-MONOMER.

Miscellaneous databases

NextBioi 976254.
PROi Q99394.

Gene expression databases

Genevestigatori Q99394.

Family and domain databases

InterProi IPR024096. NO_sig/Golgi_transp_ligand-bd.
IPR007194. TRAPP_component.
[Graphical view ]
Pfami PF04051. TRAPP. 1 hit.
[Graphical view ]
SUPFAMi SSF111126. SSF111126. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing and analysis of 51 kb on the right arm of chromosome XV from Saccharomyces cerevisiae reveals 30 open reading frames."
    Wiemann S., Rechmann S., Benes V., Voss H., Schwager C., Vlcek C., Stegemann J., Zimmermann J., Erfle H., Paces V., Ansorge W.
    Yeast 12:281-288(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion."
    Sacher M., Jiang Y., Barrowman J., Scarpa A., Burston J., Zhang L., Schieltz D., Yates J.R. III, Abeliovich H., Ferro-Novick S.
    EMBO J. 17:2494-2503(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TRAPP II COMPLEX.
  6. "TRAPP I implicated in the specificity of tethering in ER-to-Golgi transport."
    Sacher M., Barrowman J., Wang W., Horecka J., Zhang Y., Pypaert M., Ferro-Novick S.
    Mol. Cell 7:433-442(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE TRAPP II COMPLEX, IDENTIFICATION IN THE TRAPP II COMPLEX, FUNCTION OF THE TRAPP I COMPLEX, IDENTIFICATION IN THE TRAPP I COMPLEX, SUBCELLULAR LOCATION.
  7. "Molecular architecture of the TRAPPII complex and implications for vesicle tethering."
    Yip C.K., Berscheminski J., Walz T.
    Nat. Struct. Mol. Biol. 17:1298-1304(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TRAP II COMPLEX, FUNCTION OF THE TRAP II COMPLEX.
  8. Cited for: IDENTIFICATION IN THE TRAPP III COMPLEX, SUBCELLULAR LOCATION, FUNCTION OF THE TRAPP III COMPLEX.
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTRS33_YEAST
AccessioniPrimary (citable) accession number: Q99394
Secondary accession number(s): D6W2H4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3