Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vacuolar calcium ion transporter

Gene

VCX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in promoting intracellular calcium ion sequestration via the exchange of calcium ions for hydrogen ions across the vacuolar membrane. Involved also in manganese ion homeostasis via its uptake into the vacuole.2 Publications

GO - Molecular functioni

  • calcium:proton antiporter activity Source: SGD
  • metal ion binding Source: UniProtKB-KW
  • potassium:proton antiporter activity Source: SGD

GO - Biological processi

  • calcium ion transport Source: SGD
  • cellular calcium ion homeostasis Source: SGD
  • transmembrane transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29527-MONOMER.

Protein family/group databases

TCDBi2.A.19.2.2. the ca(2+):cation antiporter (caca) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar calcium ion transporter
Alternative name(s):
High copy number undoes manganese protein 1
Manganese resistance 1 protein
Vacuolar Ca(2+)/H(+) exchanger
Gene namesi
Name:VCX1
Synonyms:HUM1, MNR1
Ordered Locus Names:YDL128W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL128W.
SGDiS000002286. VCX1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30CytoplasmicSequence analysisAdd BLAST30
Transmembranei31 – 53HelicalSequence analysisAdd BLAST23
Topological domaini54 – 59VacuolarSequence analysis6
Transmembranei60 – 82HelicalSequence analysisAdd BLAST23
Topological domaini83 – 91CytoplasmicSequence analysis9
Transmembranei92 – 114HelicalSequence analysisAdd BLAST23
Topological domaini115 – 126VacuolarSequence analysisAdd BLAST12
Transmembranei127 – 146HelicalSequence analysisAdd BLAST20
Topological domaini147 – 160CytoplasmicSequence analysisAdd BLAST14
Transmembranei161 – 183HelicalSequence analysisAdd BLAST23
Topological domaini184 – 203VacuolarSequence analysisAdd BLAST20
Transmembranei204 – 226HelicalSequence analysisAdd BLAST23
Topological domaini227 – 252CytoplasmicSequence analysisAdd BLAST26
Transmembranei253 – 275HelicalSequence analysisAdd BLAST23
Topological domaini276 – 287VacuolarSequence analysisAdd BLAST12
Transmembranei288 – 310HelicalSequence analysisAdd BLAST23
Topological domaini311 – 319CytoplasmicSequence analysis9
Transmembranei320 – 342HelicalSequence analysisAdd BLAST23
Topological domaini343 – 348VacuolarSequence analysis6
Transmembranei349 – 371HelicalSequence analysisAdd BLAST23
Topological domaini372 – 380CytoplasmicSequence analysis9
Transmembranei381 – 398HelicalSequence analysisAdd BLAST18
Topological domaini399 – 411VacuolarSequence analysisAdd BLAST13

GO - Cellular componenti

  • fungal-type vacuole Source: SGD
  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002095041 – 411Vacuolar calcium ion transporterAdd BLAST411

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei13PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ99385.
PRIDEiQ99385.

PTM databases

iPTMnetiQ99385.

Interactioni

Protein-protein interaction databases

BioGridi31935. 25 interactors.
DIPiDIP-5039N.
MINTiMINT-523645.

Structurei

Secondary structure

1411
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 36Combined sources12
Helixi41 – 45Combined sources5
Helixi46 – 56Combined sources11
Helixi60 – 87Combined sources28
Helixi91 – 100Combined sources10
Helixi101 – 103Combined sources3
Helixi104 – 114Combined sources11
Turni115 – 117Combined sources3
Helixi119 – 134Combined sources16
Helixi136 – 146Combined sources11
Helixi158 – 181Combined sources24
Helixi193 – 219Combined sources27
Turni220 – 222Combined sources3
Helixi223 – 241Combined sources19
Helixi250 – 273Combined sources24
Helixi275 – 281Combined sources7
Helixi286 – 292Combined sources7
Helixi294 – 311Combined sources18
Helixi315 – 331Combined sources17
Helixi333 – 343Combined sources11
Helixi354 – 373Combined sources20
Helixi378 – 396Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4K1CX-ray2.30A/B1-411[»]
ProteinModelPortaliQ99385.
SMRiQ99385.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000083011.
InParanoidiQ99385.
KOiK07300.
OMAiWDSTAVF.
OrthoDBiEOG092C2NQI.

Family and domain databases

InterProiIPR004713. CaH_exchang.
IPR004798. CAX.
IPR004837. NaCa_Exmemb.
IPR030177. Vcx1.
[Graphical view]
PANTHERiPTHR31503:SF5. PTHR31503:SF5. 1 hit.
PfamiPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00846. caca2. 1 hit.
TIGR00378. cax. 1 hit.

Sequencei

Sequence statusi: Complete.

Q99385-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDATTPLLTV ANSHPARNPK HTAWRAAVYD LQYILKASPL NFLLVFVPLG
60 70 80 90 100
LIWGHFQLSH TLTFLFNFLA IIPLAAILAN ATEELADKAG NTIGGLLNAT
110 120 130 140 150
FGNAVELIVS IIALKKGQVR IVQASMLGSL LSNLLLVLGL CFIFGGYNRV
160 170 180 190 200
QQTFNQTAAQ TMSSLLAIAC ASLLIPAAFR ATLPHGKEDH FIDGKILELS
210 220 230 240 250
RGTSIVILIV YVLFLYFQLG SHHALFEQQE EETDEVMSTI SRNPHHSLSV
260 270 280 290 300
KSSLVILLGT TVIISFCADF LVGTIDNVVE STGLSKTFIG LIVIPIVGNA
310 320 330 340 350
AEHVTSVLVA MKDKMDLALG VAIGSSLQVA LFVTPFMVLV GWMIDVPMTL
360 370 380 390 400
NFSTFETATL FIAVFLSNYL ILDGESNWLE GVMSLAMYIL IAMAFFYYPD
410
EKTLDSIGNS L
Length:411
Mass (Da):44,646
Last modified:November 1, 1996 - v1
Checksum:i4A946818A02E8078
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti204S → A in manganese-resistant mutant 2. 1
Natural varianti208L → P in manganese-resistant mutant 1. 1
Natural varianti383M → I in VCX1-D1. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18944 Genomic DNA. Translation: AAC49550.1.
U36603 Genomic DNA. Translation: AAB60313.1.
AJ001272 Genomic DNA. Translation: CAA04645.1.
AJ001273 Genomic DNA. Translation: CAA04646.1.
Z74176 Genomic DNA. Translation: CAA98696.1.
BK006938 Genomic DNA. Translation: DAA11732.1.
PIRiS61933.
RefSeqiNP_010155.1. NM_001180187.1.

Genome annotation databases

EnsemblFungiiYDL128W; YDL128W; YDL128W.
GeneIDi851429.
KEGGisce:YDL128W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18944 Genomic DNA. Translation: AAC49550.1.
U36603 Genomic DNA. Translation: AAB60313.1.
AJ001272 Genomic DNA. Translation: CAA04645.1.
AJ001273 Genomic DNA. Translation: CAA04646.1.
Z74176 Genomic DNA. Translation: CAA98696.1.
BK006938 Genomic DNA. Translation: DAA11732.1.
PIRiS61933.
RefSeqiNP_010155.1. NM_001180187.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4K1CX-ray2.30A/B1-411[»]
ProteinModelPortaliQ99385.
SMRiQ99385.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31935. 25 interactors.
DIPiDIP-5039N.
MINTiMINT-523645.

Protein family/group databases

TCDBi2.A.19.2.2. the ca(2+):cation antiporter (caca) family.

PTM databases

iPTMnetiQ99385.

Proteomic databases

MaxQBiQ99385.
PRIDEiQ99385.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL128W; YDL128W; YDL128W.
GeneIDi851429.
KEGGisce:YDL128W.

Organism-specific databases

EuPathDBiFungiDB:YDL128W.
SGDiS000002286. VCX1.

Phylogenomic databases

HOGENOMiHOG000083011.
InParanoidiQ99385.
KOiK07300.
OMAiWDSTAVF.
OrthoDBiEOG092C2NQI.

Enzyme and pathway databases

BioCyciYEAST:G3O-29527-MONOMER.

Miscellaneous databases

PROiQ99385.

Family and domain databases

InterProiIPR004713. CaH_exchang.
IPR004798. CAX.
IPR004837. NaCa_Exmemb.
IPR030177. Vcx1.
[Graphical view]
PANTHERiPTHR31503:SF5. PTHR31503:SF5. 1 hit.
PfamiPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00846. caca2. 1 hit.
TIGR00378. cax. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVCX1_YEAST
AccessioniPrimary (citable) accession number: Q99385
Secondary accession number(s): D6VRM2, O13580, O13581
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7770 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.