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Reviewed, UniProtKB/Swiss-Prot Q99385 (VCX1_YEAST)

Last modified November 24, 2009. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Vacuolar calcium ion transporter
Alternative name(s):
    Vacuolar Ca(2+)/H(+) exchanger
    High copy number undoes manganese protein 1
    Manganese resistance 1 protein
Gene names
Name: VCX1
Synonyms: HUM1, MNR1
Ordered Locus Names: YDL128W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length411 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Has a role in promoting intracellular calcium ion sequestration via the exchange of calcium ions for hydrogen ions across the vacuolar membrane. Involved also in manganese ion homeostasis via its uptake into the vacuole. Ref.1 Ref.2

Subcellular location

Vacuole membrane; Multi-pass membrane protein.

Miscellaneous

Present with 7770 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the sodium/potassium/calcium exchanger family.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

COS1P538221EBI-37444,EBI-4959
COS4P435421EBI-37444,EBI-2050225
PHO88P382641EBI-37444,EBI-13350

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 411411Vacuolar calcium ion transporter
PRO_0000209504

Regions

Topological domain1 – 3030Cytoplasmic Potential
Transmembrane31 – 5323 Potential
Topological domain54 – 596Vacuolar Potential
Transmembrane60 – 8223 Potential
Topological domain83 – 919Cytoplasmic Potential
Transmembrane92 – 11423 Potential
Topological domain115 – 12612Vacuolar Potential
Transmembrane127 – 14620 Potential
Topological domain147 – 16014Cytoplasmic Potential
Transmembrane161 – 18323 Potential
Topological domain184 – 20320Vacuolar Potential
Transmembrane204 – 22623 Potential
Topological domain227 – 25226Cytoplasmic Potential
Transmembrane253 – 27523 Potential
Topological domain276 – 28712Vacuolar Potential
Transmembrane288 – 31023 Potential
Topological domain311 – 3199Cytoplasmic Potential
Transmembrane320 – 34223 Potential
Topological domain343 – 3486Vacuolar Potential
Transmembrane349 – 37123 Potential
Topological domain372 – 3809Cytoplasmic Potential
Transmembrane381 – 39818 Potential
Topological domain399 – 41113Vacuolar Potential

Amino acid modifications

Modified residue11N-acetylmethionine Ref.6
Modified residue131Phosphoserine Ref.6

Natural variations

Natural variant2041S → A in manganese-resistant mutant 2.
Natural variant2081L → P in manganese-resistant mutant 1.
Natural variant3831M → I in VCX1-D1. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Q99385-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 4A946818A02E8078

FASTA41144,646
        10         20         30         40         50         60 
MDATTPLLTV ANSHPARNPK HTAWRAAVYD LQYILKASPL NFLLVFVPLG LIWGHFQLSH 

        70         80         90        100        110        120 
TLTFLFNFLA IIPLAAILAN ATEELADKAG NTIGGLLNAT FGNAVELIVS IIALKKGQVR 

       130        140        150        160        170        180 
IVQASMLGSL LSNLLLVLGL CFIFGGYNRV QQTFNQTAAQ TMSSLLAIAC ASLLIPAAFR 

       190        200        210        220        230        240 
ATLPHGKEDH FIDGKILELS RGTSIVILIV YVLFLYFQLG SHHALFEQQE EETDEVMSTI 

       250        260        270        280        290        300 
SRNPHHSLSV KSSLVILLGT TVIISFCADF LVGTIDNVVE STGLSKTFIG LIVIPIVGNA 

       310        320        330        340        350        360 
AEHVTSVLVA MKDKMDLALG VAIGSSLQVA LFVTPFMVLV GWMIDVPMTL NFSTFETATL 

       370        380        390        400        410 
FIAVFLSNYL ILDGESNWLE GVMSLAMYIL IAMAFFYYPD EKTLDSIGNS L

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References

« Hide 'large scale' references
[1]"The product of HUM1, a novel yeast gene, is required for vacuolar Ca2+/H+ exchange and is related to mammalian Na+/Ca2+ exchangers."
Pozos T.C., Sekler I., Cyert M.S.
Mol. Cell. Biol. 16:3730-3741(1996) [PubMed: 8668190] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
Strain: S288c / YPH1.
[2]"Calcineurin inhibits VCX1-dependent H+/Ca2+ exchange and induces Ca2+ ATPases in Saccharomyces cerevisiae."
Cunningham K.W., Fink G.R.
Mol. Cell. Biol. 16:2226-2237(1996) [PubMed: 8628289] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, VARIANT VCX1-D1 ILE-383.
[3]"A single nucleotide change in the MNR1 (VCX1/HUM1) gene determines resistance to Manganese in Saccharomyces cerevisae."
Del Pozo L., Osaba L., Corchero J., Jimenez A.
Yeast 15:371-375(1999) [PubMed: 10219995] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTANTS MANGANESE-RESISTANT.
Strain: ATCC 201238 / W303-1B.
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed: 9169867] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION AT SER-13, MASS SPECTROMETRY.
[7]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

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Cross-references

Sequence databases

U18944 Genomic DNA. Translation: AAC49550.1.
U36603 Genomic DNA. Translation: AAB60313.1.
AJ001272 Genomic DNA. Translation: CAA04645.1.
AJ001273 Genomic DNA. Translation: CAA04646.1.
Z74176 Genomic DNA. Translation: CAA98696.1.
PIRS61933.
RefSeqNP_010155.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:5039N.
IntActQ99385. 4 interactions.
STRINGQ99385.

Protein family/group databases

TCDB2.A.19.2.2. Ca2+:cation antiporter (CaCA) family.

Genome annotation databases

EnsemblYDL128W; YDL128W; YDL128W; Saccharomyces cerevisiae. [Genome view]
GeneID851429.
KEGGsce:YDL128W.
NMPDRfig|4932.3.peg.892.

Organism-specific databases

CYGDYDL128w.
SGDS000002286. VCX1.

Phylogenomic databases

HOGENOMQ99385.
OMAIDVPMTL
OrthoDBEOG9SN354

Gene expression databases

ArrayExpressQ99385.
GenevestigatorQ99385.
GermOnlineYDL128W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR004713. CaH_exchang.
IPR004798. Cax.
IPR004837. NaCa_Exmemb.
[Graphical view]
PfamPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
TIGRFAMsTIGR00846. caca2. 1 hit.
TIGR00378. cax. 1 hit.
ProtoNetSearch...

Other Resources

NextBio968648.

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Entry information

Entry nameVCX1_YEAST
AccessionPrimary (citable) accession number: Q99385
Secondary accession number(s): O13580, O13581
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: November 1, 1996
Last modified: November 24, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents