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Protein

Nuclear polyadenylated RNA-binding protein 4

Gene

HRP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein, which is involved in the polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with the cleavage factor CFIA complex and the cleavage and polyadenylation factor (CPF) complex. May be involved in regulation of poly(A) site selection. Is involved in nonsense-mediated mRNA decay. Seems to bind to an RNA downstream sequence element (DSE) located 3' of a nonsense codon and may mark the transcript for decay.4 Publications

GO - Molecular functioni

  • mRNA binding Source: SGD
  • nucleotide binding Source: InterPro
  • RNA binding Source: SGD

GO - Biological processi

  • mRNA polyadenylation Source: SGD
  • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB-KW
  • pre-mRNA cleavage required for polyadenylation Source: SGD
  • response to DNA damage checkpoint signaling Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, Nonsense-mediated mRNA decay

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33519-MONOMER.
ReactomeiR-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear polyadenylated RNA-binding protein 4
Alternative name(s):
Cleavage factor IB
Short name:
CFIB
Gene namesi
Name:HRP1
Synonyms:NAB4, NAB5
Ordered Locus Names:YOL123W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL123W.
SGDiS000005483. HRP1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytoplasmic stress granule Source: SGD
  • mRNA cleavage factor complex Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000816581 – 534Nuclear polyadenylated RNA-binding protein 4Add BLAST534

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2PhosphoserineCombined sources1
Modified residuei3PhosphoserineCombined sources1
Modified residuei206PhosphoserineCombined sources1
Modified residuei458PhosphothreonineCombined sources1
Modified residuei462PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99383.
PRIDEiQ99383.

PTM databases

iPTMnetiQ99383.

Interactioni

Subunit structurei

Interacts with NAM7.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NAB2P325053EBI-11783,EBI-11770

Protein-protein interaction databases

BioGridi34252. 64 interactors.
DIPiDIP-1371N.
IntActiQ99383. 33 interactors.
MINTiMINT-395353.

Structurei

Secondary structure

1534
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi157 – 159Combined sources3
Beta strandi160 – 163Combined sources4
Helixi172 – 179Combined sources8
Turni180 – 182Combined sources3
Beta strandi185 – 189Combined sources5
Turni194 – 196Combined sources3
Beta strandi203 – 209Combined sources7
Helixi211 – 218Combined sources8
Beta strandi223 – 226Combined sources4
Helixi236 – 241Combined sources6
Beta strandi243 – 250Combined sources8
Helixi256 – 264Combined sources9
Beta strandi265 – 267Combined sources3
Beta strandi270 – 273Combined sources4
Beta strandi277 – 281Combined sources5
Beta strandi284 – 293Combined sources10
Helixi294 – 302Combined sources9
Beta strandi304 – 306Combined sources3
Beta strandi308 – 311Combined sources4
Beta strandi313 – 317Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CJKNMR-A156-322[»]
2KM8NMR-C156-322[»]
ProteinModelPortaliQ99383.
SMRiQ99383.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99383.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini159 – 241RRM 1PROSITE-ProRule annotationAdd BLAST83
Domaini243 – 320RRM 2PROSITE-ProRule annotationAdd BLAST78

Sequence similaritiesi

Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00760000118873.
HOGENOMiHOG000248803.
InParanoidiQ99383.
KOiK14411.
OMAiSKESCKM.
OrthoDBiEOG092C3SCB.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99383-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDEEDFND IYGDDKPTTT EEVKKEEEQN KAGSGTSQLD QLAALQALSS
60 70 80 90 100
SLNKLNNPNS NNSSSNNSNQ DTSSSKQDGT ANDKEGSNED TKNEKKQESA
110 120 130 140 150
TSANANANAS SAGPSGLPWE QLQQTMSQFQ QPSSQSPPQQ QVTQTKEERS
160 170 180 190 200
KADLSKESCK MFIGGLNWDT TEDNLREYFG KYGTVTDLKI MKDPATGRSR
210 220 230 240 250
GFGFLSFEKP SSVDEVVKTQ HILDGKVIDP KRAIPRDEQD KTGKIFVGGI
260 270 280 290 300
GPDVRPKEFE EFFSQWGTII DAQLMLDKDT GQSRGFGFVT YDSADAVDRV
310 320 330 340 350
CQNKFIDFKD RKIEIKRAEP RHMQQKSSNN GGNNGGNNMN RRGGNFGNQG
360 370 380 390 400
DFNQMYQNPM MGGYNPMMNP QAMTDYYQKM QEYYQQMQKQ TGMDYTQMYQ
410 420 430 440 450
QQMQQMAMMM PGFAMPPNAM TLNQPQQDSN ATQGSPAPSD SDNNKSNDVQ
460 470 480 490 500
TIGNTSNTDS GSPPLNLPNG PKGPSQYNDD HNSGYGYNRD RGDRDRNDRD
510 520 530
RDYNHRSGGN HRRNGRGGRG GYNRRNNGYH PYNR
Length:534
Mass (Da):59,650
Last modified:November 1, 1997 - v1
Checksum:i1B231069437B093D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35737 Genomic DNA. Translation: AAA79097.1.
U38535 Genomic DNA. Translation: AAB18142.1.
X95258 Genomic DNA. Translation: CAA64546.1.
Z74865 Genomic DNA. Translation: CAA99142.1.
BK006948 Genomic DNA. Translation: DAA10661.1.
PIRiS66820.
RefSeqiNP_014518.1. NM_001183377.1.

Genome annotation databases

EnsemblFungiiYOL123W; YOL123W; YOL123W.
GeneIDi853997.
KEGGisce:YOL123W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35737 Genomic DNA. Translation: AAA79097.1.
U38535 Genomic DNA. Translation: AAB18142.1.
X95258 Genomic DNA. Translation: CAA64546.1.
Z74865 Genomic DNA. Translation: CAA99142.1.
BK006948 Genomic DNA. Translation: DAA10661.1.
PIRiS66820.
RefSeqiNP_014518.1. NM_001183377.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CJKNMR-A156-322[»]
2KM8NMR-C156-322[»]
ProteinModelPortaliQ99383.
SMRiQ99383.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34252. 64 interactors.
DIPiDIP-1371N.
IntActiQ99383. 33 interactors.
MINTiMINT-395353.

PTM databases

iPTMnetiQ99383.

Proteomic databases

MaxQBiQ99383.
PRIDEiQ99383.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL123W; YOL123W; YOL123W.
GeneIDi853997.
KEGGisce:YOL123W.

Organism-specific databases

EuPathDBiFungiDB:YOL123W.
SGDiS000005483. HRP1.

Phylogenomic databases

GeneTreeiENSGT00760000118873.
HOGENOMiHOG000248803.
InParanoidiQ99383.
KOiK14411.
OMAiSKESCKM.
OrthoDBiEOG092C3SCB.

Enzyme and pathway databases

BioCyciYEAST:G3O-33519-MONOMER.
ReactomeiR-SCE-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.

Miscellaneous databases

EvolutionaryTraceiQ99383.
PROiQ99383.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHRP1_YEAST
AccessioniPrimary (citable) accession number: Q99383
Secondary accession number(s): D6W1U5, Q02741
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.