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Q99383 (HRP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear polyadenylated RNA-binding protein 4
Alternative name(s):
Cleavage factor IB
Short name=CFIB
Gene names
Name:HRP1
Synonyms:NAB4, NAB5
Ordered Locus Names:YOL123W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length534 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA-binding protein, which is involved in the polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with the cleavage factor CFIA complex and the cleavage and polyadenylation factor (CPF) complex. May be involved in regulation of poly(A) site selection. Is involved in nonsense-mediated mRNA decay. Seems to bind to an RNA downstream sequence element (DSE) located 3' of a nonsense codon and may mark the transcript for decay. Ref.6 Ref.7 Ref.9 Ref.10

Subunit structure

Interacts with NAM7. Ref.9

Subcellular location

Cytoplasm. Nucleus Ref.6 Ref.11.

Sequence similarities

Contains 2 RRM (RNA recognition motif) domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

NAB2P325053EBI-11783,EBI-11770

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 534534Nuclear polyadenylated RNA-binding protein 4
PRO_0000081658

Regions

Domain159 – 24183RRM 1
Domain243 – 32078RRM 2

Amino acid modifications

Modified residue21Phosphoserine Ref.12 Ref.13 Ref.14
Modified residue31Phosphoserine Ref.12 Ref.13 Ref.14
Modified residue2061Phosphoserine Ref.13
Modified residue4581Phosphothreonine Ref.13
Modified residue4621Phosphoserine Ref.13 Ref.14

Secondary structure

..................................... 534
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q99383 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 1B231069437B093D

FASTA53459,650
        10         20         30         40         50         60 
MSSDEEDFND IYGDDKPTTT EEVKKEEEQN KAGSGTSQLD QLAALQALSS SLNKLNNPNS 

        70         80         90        100        110        120 
NNSSSNNSNQ DTSSSKQDGT ANDKEGSNED TKNEKKQESA TSANANANAS SAGPSGLPWE 

       130        140        150        160        170        180 
QLQQTMSQFQ QPSSQSPPQQ QVTQTKEERS KADLSKESCK MFIGGLNWDT TEDNLREYFG 

       190        200        210        220        230        240 
KYGTVTDLKI MKDPATGRSR GFGFLSFEKP SSVDEVVKTQ HILDGKVIDP KRAIPRDEQD 

       250        260        270        280        290        300 
KTGKIFVGGI GPDVRPKEFE EFFSQWGTII DAQLMLDKDT GQSRGFGFVT YDSADAVDRV 

       310        320        330        340        350        360 
CQNKFIDFKD RKIEIKRAEP RHMQQKSSNN GGNNGGNNMN RRGGNFGNQG DFNQMYQNPM 

       370        380        390        400        410        420 
MGGYNPMMNP QAMTDYYQKM QEYYQQMQKQ TGMDYTQMYQ QQMQQMAMMM PGFAMPPNAM 

       430        440        450        460        470        480 
TLNQPQQDSN ATQGSPAPSD SDNNKSNDVQ TIGNTSNTDS GSPPLNLPNG PKGPSQYNDD 

       490        500        510        520        530 
HNSGYGYNRD RGDRDRNDRD RDYNHRSGGN HRRNGRGGRG GYNRRNNGYH PYNR 

« Hide

References

« Hide 'large scale' references
[1]Oberdorf A.M., Anderson J.T., Devore D.R., Swanson M.S.
Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Potential RNA binding proteins in Saccharomyces cerevisiae identified as suppressors of temperature-sensitive mutations in NPL3."
Henry M., Borland C.Z., Bossie M., Silver P.A.
Genetics 142:103-115(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"DNA sequence analysis of a 10 624 bp fragment of the left arm of chromosome XV from Saccharomyces cerevisiae reveals a RNA binding protein, a mitochondrial protein, two ribosomal proteins and two new open reading frames."
Lafuente M.J., Gamo F.-J., Gancedo C.
Yeast 12:1041-1045(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Hrp1, a sequence-specific RNA-binding protein that shuttles between the nucleus and the cytoplasm, is required for mRNA 3'-end formation in yeast."
Kessler M.M., Henry M.F., Shen E., Zhao J., Gross S., Silver P.A., Moore C.L.
Genes Dev. 11:2545-2556(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 182-189 AND 245-257, FUNCTION, RNA-BINDING, SUBCELLULAR LOCATION.
[7]"Control of cleavage site selection during mRNA 3' end formation by a yeast hnRNP."
Minvielle-Sebastia L., Beyer K., Krecic A.M., Hector R.E., Swanson M.S., Keller W.
EMBO J. 17:7454-7468(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"A specific RNA-protein interaction at yeast polyadenylation efficiency elements."
Chen S., Hyman L.E.
Nucleic Acids Res. 26:4965-4974(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: RNA-BINDING.
[9]"The yeast hnRNP-like protein Hrp1/Nab4 marks a transcript for nonsense-mediated mRNA decay."
Gonzalez C.I., Ruiz-Echevarria M.J., Vasudevan S., Henry M.F., Peltz S.W.
Mol. Cell 5:489-499(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN NMD, INTERACTION WITH NAM7.
[10]"Five subunits are required for reconstitution of the cleavage and polyadenylation activities of Saccharomyces cerevisiae cleavage factor I."
Gross S., Moore C.
Proc. Natl. Acad. Sci. U.S.A. 98:6080-6085(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN PRE-MRNA 3'-END FORMATION.
[11]"Coupling of termination, 3' processing, and mRNA export."
Hammell C.M., Gross S., Zenklusen D., Heath C.V., Stutz F., Moore C., Cole C.N.
Mol. Cell. Biol. 22:6441-6457(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[12]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2 AND SER-3, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[13]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2; SER-3; SER-206; THR-458 AND SER-462, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2; SER-3 AND SER-462, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U35737 Genomic DNA. Translation: AAA79097.1.
U38535 Genomic DNA. Translation: AAB18142.1.
X95258 Genomic DNA. Translation: CAA64546.1.
Z74865 Genomic DNA. Translation: CAA99142.1.
BK006948 Genomic DNA. Translation: DAA10661.1.
PIRS66820.
RefSeqNP_014518.1. NM_001183377.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CJKNMR-A156-322[»]
2KM8NMR-C156-322[»]
ProteinModelPortalQ99383.
SMRQ99383. Positions 117-322.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34252. 61 interactions.
DIPDIP-1371N.
IntActQ99383. 33 interactions.
MINTMINT-395353.

Proteomic databases

MaxQBQ99383.
PaxDbQ99383.
PeptideAtlasQ99383.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOL123W; YOL123W; YOL123W.
GeneID853997.
KEGGsce:YOL123W.

Organism-specific databases

CYGDYOL123w.
SGDS000005483. HRP1.

Phylogenomic databases

eggNOGCOG0724.
GeneTreeENSGT00730000110184.
HOGENOMHOG000248803.
KOK14411.
OMAYQKMQEY.
OrthoDBEOG706125.

Enzyme and pathway databases

BioCycYEAST:G3O-33519-MONOMER.

Gene expression databases

GenevestigatorQ99383.

Family and domain databases

Gene3D3.30.70.330. 2 hits.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTSM00360. RRM. 2 hits.
[Graphical view]
PROSITEPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ99383.
NextBio975491.
PROQ99383.

Entry information

Entry nameHRP1_YEAST
AccessionPrimary (citable) accession number: Q99383
Secondary accession number(s): D6W1U5, Q02741
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references