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Protein

Myosin heavy chain, non-muscle

Gene

zip

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nonmuscle myosin appears to be responsible for cellularization. Required for morphogenesis and cytokinesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi225 – 2328ATP

GO - Molecular functioni

  • ATPase activity, coupled Source: FlyBase
  • ATP binding Source: UniProtKB-KW
  • motor activity Source: FlyBase
  • myosin light chain binding Source: FlyBase

GO - Biological processi

  • anterior midgut development Source: FlyBase
  • border follicle cell migration Source: FlyBase
  • cellular response to mechanical stimulus Source: FlyBase
  • cuticle pattern formation Source: FlyBase
  • dorsal closure Source: FlyBase
  • dorsal closure, amnioserosa morphology change Source: FlyBase
  • dorsal closure, leading edge cell differentiation Source: FlyBase
  • dorsal closure, spreading of leading edge cells Source: FlyBase
  • establishment of neuroblast polarity Source: FlyBase
  • establishment of planar polarity Source: FlyBase
  • head involution Source: FlyBase
  • imaginal disc-derived wing hair organization Source: FlyBase
  • left/right axis specification Source: FlyBase
  • maintenance of protein location in cell Source: FlyBase
  • Malpighian tubule morphogenesis Source: FlyBase
  • mitotic cytokinesis Source: FlyBase
  • muscle contraction Source: FlyBase
  • myofibril assembly Source: FlyBase
  • myosin II filament assembly Source: FlyBase
  • neurogenesis Source: FlyBase
  • ovarian follicle cell migration Source: FlyBase
  • protein oligomerization Source: FlyBase
  • regulation of cell cycle Source: FlyBase
  • regulation of myoblast fusion Source: FlyBase
  • regulation of tube length, open tracheal system Source: FlyBase
  • salivary gland morphogenesis Source: FlyBase
  • sarcomere organization Source: FlyBase
  • spiracle morphogenesis, open tracheal system Source: FlyBase
  • wound healing Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-445355. Smooth Muscle Contraction.
R-DME-5627123. RHO GTPases activate PAKs.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin heavy chain, non-muscle
Alternative name(s):
Myosin II
Non-muscle MHC
Zipper protein
Gene namesi
Name:zip
ORF Names:CG15792
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0265434. zip.

Subcellular locationi

GO - Cellular componenti

  • apical cortex Source: FlyBase
  • cell-cell contact zone Source: FlyBase
  • cell cortex Source: FlyBase
  • cell leading edge Source: FlyBase
  • cleavage furrow Source: FlyBase
  • cytoskeleton Source: FlyBase
  • cytosol Source: FlyBase
  • myosin complex Source: FlyBase
  • myosin II complex Source: FlyBase
  • unconventional myosin complex Source: FlyBase
  • Z disc Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 20572057Myosin heavy chain, non-musclePRO_0000123388Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2021 – 20211Phosphoserine1 Publication
Modified residuei2022 – 20221Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ99323.
PeptideAtlasiQ99323.
PRIDEiQ99323.

PTM databases

iPTMnetiQ99323.

Expressioni

Developmental stagei

Isoforms containing exon 7 are detected in all stages of development and are expressed in embryos, early and late larvae, and adults.1 Publication

Gene expression databases

BgeeiFBgn0005634.
ExpressionAtlasiQ99323. differential.
GenevisibleiQ99323. DM.

Interactioni

Subunit structurei

Interacts with sau.1 Publication

GO - Molecular functioni

  • myosin light chain binding Source: FlyBase

Protein-protein interaction databases

BioGridi63569. 49 interactions.
IntActiQ99323. 13 interactions.
MINTiMINT-913393.
STRINGi7227.FBpp0072306.

Structurei

3D structure databases

ProteinModelPortaliQ99323.
SMRiQ99323. Positions 58-988.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini132 – 867736Myosin motorAdd
BLAST
Domaini870 – 89930IQPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni250 – 2601125 kDa/50 kDa junctionAdd
BLAST
Regioni722 – 7341350 kDa/20 kDa junctionAdd
BLAST
Regioni745 – 76723Actin-bindingAdd
BLAST
Regioni782 – 79817Reactive sulfhydryl/actin-bindingAdd
BLAST
Regioni1343 – 2057715Light meromyosin (LMM)Add
BLAST
Regioni1343 – 2010668Alpha-helical tailpiece (LMM)Add
BLAST
Regioni2011 – 205747Globular tailpieceAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili926 – 20161091Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 IQ domain.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0161. Eukaryota.
COG5022. LUCA.
InParanoidiQ99323.
KOiK10352.
OrthoDBiEOG091G009J.
PhylomeDBiQ99323.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00612. IQ. 1 hit.
PF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 1 hit.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3 (identifier: Q99323-3) [UniParc]FASTAAdd to basket
Also known as: a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKSAWLALKS KSALHPKSEY PVSNIHYPKA ANYRQTRNYL EIAAKMSEEV
60 70 80 90 100
DRNDPELKYL SVERNQFNPI RPRRPSGHRS VLVWVPHENQ GFVAASIKRE
110 120 130 140 150
HGDEVEVELA ETGKRVMILR DDIQKMNPPK FDKVEDMAEL TCLNEASVLH
160 170 180 190 200
NIKDRYYSGL IYTYSGLFCV VVNPYKKLPI YTEKIMERYK GIKRHEVPPH
210 220 230 240 250
VFAITDSAYR NMLGDREDQS ILCTGESGAG KTENTKKVIQ FLAYVAASKP
260 270 280 290 300
KGSGAVPHPA VLINFSVNTN KYIKVKIMAQ NQNQTIEVVN GLKMVEVNSN
310 320 330 340 350
CQEGELEQQL LQANPILEAF GNAKTVKNDN SSRFGKFIRI NFDASGFISG
360 370 380 390 400
ANIETYLLEK SRAIRQAKDE RTFHIFYQLL AGATPEQREK FILDDVKSYA
410 420 430 440 450
FLSNGSLPVP GVDDYAEFQA TVKSMNIMGM TSEDFNSIFR IVSAVLLFGS
460 470 480 490 500
MKFRQERNND QATLPDNTVA QKIAHLLGLS VTDMTRAFLT PRIKVGRDFV
510 520 530 540 550
TKAQTKEQVE FAVEAIAKAC YERMFKWLVN RINRSLDRTK RQGASFIGIL
560 570 580 590 600
DMAGFEIFEL NSFEQLCINY TNEKLQQLFN HTMFILEQEE YQREGIEWKF
610 620 630 640 650
IDFGLDLQPT IDLIDKPGGI MALLDEECWF PKATDKTFVD KLVSAHSMHP
660 670 680 690 700
KFMKTDFRGV ADFAIVHYAG RVDYSAAKWL MKNMDPLNEN IVSLLQGSQD
710 720 730 740 750
PFVVNIWKDA EIVGMAQQAL TDTQFGARTR KGMFRTVSHL YKEQLAKLMD
760 770 780 790 800
TLRNTNPNFV RCIIPNHEKR AGKIDAPLVL DQLRCNGVLE GIRICRQGFP
810 820 830 840 850
NRIPFQEFRQ RYELLTPNVI PKGFMDGKKA CEKMIQALEL DSNLYRVGQS
860 870 880 890 900
KIFFRAGVLA HLEEERDFKI SDLIVNFQAF CRGFLARRNY QKRLQQLNAI
910 920 930 940 950
RIIQRNCAAY LKLRNWQWWR LYTKVKPLLE VTKQEEKLVQ KEDELKQVRE
960 970 980 990 1000
KLDTLAKNTQ EYERKYQQAL VEKTTLAEQL QAEIELCAEA EESRSRLMAR
1010 1020 1030 1040 1050
KQELEDMMQE LETRIEEEEE RVLALGGEKK KLELNIQDLE EQLEEEEAAR
1060 1070 1080 1090 1100
QKLQLEKVQL DAKIKKYEED LALTDDQNQK LLKEKKLLEE RANDLSQTLA
1110 1120 1130 1140 1150
EEEEKAKHLA KLKAKHEATI TELEERLHKD QQQRQESDRS KRKIETEVAD
1160 1170 1180 1190 1200
LKEQLNERRV QVDEMQAQLA KREEELTQTL LRIDEESATK ATAQKAQREL
1210 1220 1230 1240 1250
ESQLAEIQED LEAEKAARAK AEKVRRDLSE ELEALKNELL DSLDTTAAQQ
1260 1270 1280 1290 1300
ELRSKREQEL ATLKKSLEEE TVNHEGVLAD MRHKHSQELN SINDQLENLR
1310 1320 1330 1340 1350
KAKTVLEKAK GTLEAENADL ATELRSVNSS RQENDRRRKQ AESQIAELQV
1360 1370 1380 1390 1400
KLAEIERARS ELQEKCTKLQ QEAENITNQL EEAELKASAA VKSASNMESQ
1410 1420 1430 1440 1450
LTEAQQLLEE ETRQKLGLSS KLRQIESEKE ALQEQLEEDD EAKRNYERKL
1460 1470 1480 1490 1500
AEVTTQMQEI KKKAEEDADL AKELEEGKKR LNKDIEALER QVKELIAQND
1510 1520 1530 1540 1550
RLDKSKKKIQ SELEDATIEL EAQRTKVLEL EKKQKNFDKI LAEEKAISEQ
1560 1570 1580 1590 1600
IAQERDTAER EAREKETKVL SVSRELDEAF DKIEDLENKR KTLQNELDDL
1610 1620 1630 1640 1650
ANTQGTADKN VHELEKAKRA LESQLAELKA QNEELEDDLQ LTEDAKLRLE
1660 1670 1680 1690 1700
VNMQALRSQF ERDLLAKEEG AEEKRRGLVK QLRDLETELD EERKQRTAAV
1710 1720 1730 1740 1750
ASKKKLEGDL KEIETTMEMH NKVKEDALKH AKKLQAQVKD ALRDAEEAKA
1760 1770 1780 1790 1800
AKEELQALSK EADGKVKALE AEVLQLTEDL ASSERARRAA ETERDELAEE
1810 1820 1830 1840 1850
IANNANKGSL MIDEKRRLEA RIATLEEELE EEQSNSEVLL DRSRKAQLQI
1860 1870 1880 1890 1900
EQLTTELANE KSNSQKNENG RALLERQNKE LKAKLAEIET AQRTKVKATI
1910 1920 1930 1940 1950
ATLEAKIANL EEQLENEGKE RLLQQKANRK MDKKIKELTM NIEDERRHVD
1960 1970 1980 1990 2000
QHKEQMDKLN SRIKLLKRNL DETEEELQKE KTQKRKYQRE CEDMIESQEA
2010 2020 2030 2040 2050
MNREINSLKT KLRRTGGIGL SSSRLTGTPS SKRAGGGGGS DDSSVQDESL

DGEDSAN
Length:2,057
Mass (Da):236,644
Last modified:August 16, 2004 - v2
Checksum:i0D59519C1E83A411
GO
Isoform 1 (identifier: Q99323-1) [UniParc]FASTAAdd to basket
Also known as: Long

The sequence of this isoform differs from the canonical sequence as follows:
     264-303: Missing.

Show »
Length:2,017
Mass (Da):232,091
Checksum:i8C76FEF2EBD02EBE
GO
Isoform 2 (identifier: Q99323-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.
     264-303: Missing.

Show »
Length:1,972
Mass (Da):226,933
Checksum:i356FE84391412722
GO
Isoform 4 (identifier: Q99323-4) [UniParc]FASTAAdd to basket
Also known as: b

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.

Show »
Length:2,012
Mass (Da):231,486
Checksum:i0AB94A7865483E2F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti69 – 8113PIRPR…GHRSV → DPATQAEWTQKR (PubMed:10731132).CuratedAdd
BLAST
Sequence conflicti1121 – 11211T → S in AAF47311 (PubMed:10731132).Curated
Sequence conflicti1121 – 11211T → S in AAM70805 (PubMed:10731132).Curated
Sequence conflicti1763 – 17642DG → ER in AAF47311 (PubMed:10731132).Curated
Sequence conflicti1763 – 17642DG → ER in AAM70805 (PubMed:10731132).Curated
Sequence conflicti1843 – 18464SRKA → AAAR in AAA28713 (PubMed:2117279).Curated
Sequence conflicti1909 – 19102NL → KV in AAA28713 (PubMed:2117279).Curated
Sequence conflicti2040 – 20401S → G in AAF47311 (PubMed:10731132).Curated
Sequence conflicti2040 – 20401S → G in AAM70805 (PubMed:10731132).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4545Missing in isoform 2 and isoform 4. 1 PublicationVSP_003342Add
BLAST
Alternative sequencei264 – 30340Missing in isoform 1 and isoform 2. 1 PublicationVSP_011159Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35012 mRNA. Translation: AAA28713.1.
U35816 Genomic DNA. Translation: AAB09048.1.
U35816 Genomic DNA. Translation: AAB09049.1.
U35816 Genomic DNA. Translation: AAB09050.1.
U35816 Genomic DNA. Translation: AAB09051.1.
AE013599 Genomic DNA. Translation: AAF47311.1.
AE013599 Genomic DNA. Translation: AAM70805.1.
PIRiA36014.
S61477.
RefSeqiNP_523860.2. NM_079136.4.
NP_726506.1. NM_166704.4.
UniGeneiDm.2632.

Genome annotation databases

GeneIDi38001.
KEGGidme:Dmel_CG15792.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35012 mRNA. Translation: AAA28713.1.
U35816 Genomic DNA. Translation: AAB09048.1.
U35816 Genomic DNA. Translation: AAB09049.1.
U35816 Genomic DNA. Translation: AAB09050.1.
U35816 Genomic DNA. Translation: AAB09051.1.
AE013599 Genomic DNA. Translation: AAF47311.1.
AE013599 Genomic DNA. Translation: AAM70805.1.
PIRiA36014.
S61477.
RefSeqiNP_523860.2. NM_079136.4.
NP_726506.1. NM_166704.4.
UniGeneiDm.2632.

3D structure databases

ProteinModelPortaliQ99323.
SMRiQ99323. Positions 58-988.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63569. 49 interactions.
IntActiQ99323. 13 interactions.
MINTiMINT-913393.
STRINGi7227.FBpp0072306.

PTM databases

iPTMnetiQ99323.

Proteomic databases

PaxDbiQ99323.
PeptideAtlasiQ99323.
PRIDEiQ99323.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi38001.
KEGGidme:Dmel_CG15792.

Organism-specific databases

CTDi38001.
FlyBaseiFBgn0265434. zip.

Phylogenomic databases

eggNOGiKOG0161. Eukaryota.
COG5022. LUCA.
InParanoidiQ99323.
KOiK10352.
OrthoDBiEOG091G009J.
PhylomeDBiQ99323.

Enzyme and pathway databases

ReactomeiR-DME-445355. Smooth Muscle Contraction.
R-DME-5627123. RHO GTPases activate PAKs.

Miscellaneous databases

ChiTaRSizip. fly.
GenomeRNAii38001.
PROiQ99323.

Gene expression databases

BgeeiFBgn0005634.
ExpressionAtlasiQ99323. differential.
GenevisibleiQ99323. DM.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00612. IQ. 1 hit.
PF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 1 hit.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYSN_DROME
AccessioniPrimary (citable) accession number: Q99323
Secondary accession number(s): Q24138
, Q7JPC1, Q7JPC2, Q8MMC2, Q94987, Q94992, Q9W0W8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: August 16, 2004
Last modified: September 7, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.