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Protein

Diphosphoinositol polyphosphate phosphohydrolase DDP1

Gene

DDP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May eliminate potentially toxic dinucleoside polyphosphates during sporulation. Most active against diadenosine 5',5'''-P1,P6-hexaphosphate (Ap6A). Can also hydrolyze diadenosine 5',5'''-P1,P5-pentaphosphate (Ap5A), adenosine 5'-pentaphosphate, and adenosine 5'-tetraphosphate are also substrates, but not diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) or other dinucleotides, mononucleotides, nucleotide sugars, or nucleotide alcohols. Also cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate) and [PP]2-InsP4 (bisdiphosphoinositol tetrakisphosphate).

Miscellaneous

Present with 3340 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Diphospho-myo-inositol polyphosphate + H2O = myo-inositol polyphosphate + phosphate.1 Publication
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O = adenosine 5'-pentaphosphate + AMP.1 Publication
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O = adenosine 5'-tetraphosphate + AMP.1 Publication

Cofactori

Kineticsi

  1. KM=31 nM for PP-InsP51 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi80MagnesiumBy similarity1
    Metal bindingi84MagnesiumBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase
    LigandMagnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:YOR163W-MONOMER
    BRENDAi3.6.1.B13 984
    SABIO-RKiQ99321

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diphosphoinositol polyphosphate phosphohydrolase DDP1 (EC:3.6.1.52)
    Alternative name(s):
    Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase
    Short name:
    Ap6A hydrolase
    Diadenosine and diphosphoinositol polyphosphate phosphohydrolase 1
    Diadenosine hexaphosphate hydrolase (AMP-forming) (EC:3.6.1.60)
    Gene namesi
    Name:DDP1
    Ordered Locus Names:YOR163W
    ORF Names:O3575
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi

    Organism-specific databases

    EuPathDBiFungiDB:YOR163W
    SGDiS000005689 DDP1

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00000570672 – 188Diphosphoinositol polyphosphate phosphohydrolase DDP1Add BLAST187

    Proteomic databases

    MaxQBiQ99321
    PaxDbiQ99321
    PRIDEiQ99321

    PTM databases

    iPTMnetiQ99321

    Interactioni

    Protein-protein interaction databases

    BioGridi34559, 69 interactors
    IntActiQ99321, 3 interactors
    STRINGi4932.YOR163W

    Structurei

    3D structure databases

    ProteinModelPortaliQ99321
    SMRiQ99321
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini30 – 179Nudix hydrolasePROSITE-ProRule annotationAdd BLAST150

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi65 – 86Nudix boxAdd BLAST22

    Sequence similaritiesi

    Belongs to the Nudix hydrolase family. DIPP subfamily.Curated

    Phylogenomic databases

    HOGENOMiHOG000165198
    InParanoidiQ99321
    KOiK07766
    OMAiKGWVLPK
    OrthoDBiEOG092C5RKD

    Family and domain databases

    InterProiView protein in InterPro
    IPR015797 NUDIX_hydrolase-like_dom_sf
    IPR000086 NUDIX_hydrolase_dom
    PfamiView protein in Pfam
    PF00293 NUDIX, 1 hit
    SUPFAMiSSF55811 SSF55811, 1 hit
    PROSITEiView protein in PROSITE
    PS51462 NUDIX, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q99321-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGKTADNHGP VRSETAREGR ENQVYSPVTG ARLVAGCICL TPDKKQVLMI
    60 70 80 90 100
    TSSAHKKRWI VPKGGVEKDE PNYETTAQRE TWEEAGCIGK IVANLGTVED
    110 120 130 140 150
    MRPPKDWNKD IKQFENSRKD SEVAKHPPRT EFHFYELEIE NLLDKFPECH
    160 170 180
    KRHRKLYSYT EAKQNLIDAK RPELLEALNR SAIIKDDK
    Length:188
    Mass (Da):21,572
    Last modified:January 23, 2007 - v3
    Checksum:i17A39EB060F03184
    GO

    Mass spectrometryi

    Molecular mass is 21443 Da from positions 2 - 188. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z75071 Genomic DNA Translation: CAA99369.1
    U55021 Genomic DNA Translation: AAB47410.1
    AY558436 Genomic DNA Translation: AAS56762.1
    BK006948 Genomic DNA Translation: DAA10937.1
    PIRiS67051
    RefSeqiNP_014806.1, NM_001183582.1

    Genome annotation databases

    EnsemblFungiiYOR163W; YOR163W; YOR163W
    GeneIDi854334
    KEGGisce:YOR163W

    Similar proteinsi

    Entry informationi

    Entry nameiDDP1_YEAST
    AccessioniPrimary (citable) accession number: Q99321
    Secondary accession number(s): D6W2M1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: January 23, 2007
    Last modified: May 23, 2018
    This is version 159 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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