Q99316 (MPD2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein disulfide isomerase MPD2
EC=5.3.4.1

Gene names

Name:	MPD2
Ordered Locus Names:	YOL088C
ORF Names:	O0941

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Non essential disulfide isomerase, which participates in the folding of proteins containing disulfide bonds. May be involved in glycosylation, prolyl hydroxylation and triglyceride transfer. Able to fold proteins by being directly oxidized by ERO1. Ref.1
Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Endoplasmic reticulum Ref.6.
Miscellaneous	Present with 1520 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Signal
Molecular function	Isomerase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum Inferred from genetic interaction PubMed 15377672. Source: SGD
Molecular_function	protein disulfide isomerase activity Inferred from direct assay PubMed 16002399. Source: SGD protein disulfide oxidoreductase activity Inferred from direct assay PubMed 16002399. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 277	255	Protein disulfide isomerase MPD2		PRO_0000034182
Regions
	Domain	27 – 172	146	Thioredoxin
	Motif	274 – 277	4	Prevents secretion from ER Potential
Amino acid modifications
	Disulfide bond	56 ↔ 59		Redox-active By similarity
Experimental info
	Mutagenesis	56	1	C → S: Loss of function; when associated with S-59. Ref.1
	Mutagenesis	59	1	C → S: Loss of function; when associated with S-56. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q99316 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: ECCEA7C6CBB10488	FASTA	277	32,401
	10 20 30 40 50 60 MKLHGFLFSV LSTCVVILPA LAYSEAVTMV KSIEQYFDIC NRNDSYTMIK YYTSWCQHCK 70 80 90 100 110 120 TLAPVYEELG ELYAKKANKD DTPINFLEVN CEFFGPTLCT DLPGFPIIEL VKPRTKPLVL 130 140 150 160 170 180 PKLDWSSMKF HERLWQRIKT WFNNPKYQLD TSRVVRFEGS RNLKSLSNFI DTVRSKDTEE 190 200 210 220 230 240 RFIEHIFDDS RNCNEELRSQ QLLCKAGKEY YSDTLSKLYG DVNGLEKERR RLEALIKQNG 250 260 270 DDLSKEVKEK LKIIRLQLSL LSHIEDQLED TSSHDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Overproduction of Mpd2p suppresses the lethality of protein disulfide isomerase depletion in a CXXC sequence dependent manner." Tachikawa H., Funahashi W., Takeuchi Y., Nakanishi H., Nishihara R., Katoh S., Gao X.D., Mizunaga T., Fujimoto D. Biochem. Biophys. Res. Commun. 239:710-714(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF CYS-56 AND CYS-59.
[2]	"A 29.425 kb segment on the left arm of yeast chromosome XV contains more than twice as many unknown as known open reading frames." Zumstein E., Pearson B.M., Kalogeropoulos A., Schweizer M. Yeast 11:975-986(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Ero1p oxidizes protein disulfide isomerase in a pathway for disulfide bond formation in the endoplasmic reticulum." Frand A.R., Kaiser C.A. Mol. Cell 4:469-477(1999) [PubMed] [Europe PMC] [Abstract] Cited for: OXIDATION BY ERO1.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D34634 Genomic DNA. Translation: BAA22222.1. X83121 Genomic DNA. Translation: CAA58191.1. Z74830 Genomic DNA. Translation: CAA99100.1. BK006948 Genomic DNA. Translation: DAA10696.1.
PIR	S57381.
RefSeq	NP_014553.1. NM_001183342.1.
3D structure databases
ProteinModelPortal	Q99316.
SMR	Q99316. Positions 24-143.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1451N.
IntAct	Q99316. 5 interactions.
MINT	MINT-392339.
STRING	4932.YOL088C.
Proteomic databases
PaxDb	Q99316.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOL088C; YOL088C; YOL088C.
GeneID	854065.
KEGG	sce:YOL088C.
Organism-specific databases
CYGD	YOL088c.
SGD	S000005448. MPD2.
Phylogenomic databases
eggNOG	NOG314639.
HOGENOM	HOG000113635.
OMA	WCSHCKK.
OrthoDB	EOG4BP4NG.
Gene expression databases
Genevestigator	Q99316.
GermOnline	YOL088C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.30.10. 2 hits.
InterPro	IPR012336. Thioredoxin-like_fold. IPR013766. Thioredoxin_domain. [Graphical view]
Pfam	PF00085. Thioredoxin. 1 hit. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 2 hits.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00194. THIOREDOXIN_1. False negative. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975674.

Entry information

Entry name

MPD2_YEAST

Accession

Primary (citable) accession number: Q99316
Secondary accession number(s): D6W1Y0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program