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Protein

IMP-specific 5'-nucleotidase 1

Gene

ISN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

IMP-specific 5'-nucleotidase involved in IMP (inosine 5'-phosphate) degradation.2 Publications

Cofactori

pH dependencei

Optimum pH is 6-6.5.1 Publication

GO - Molecular functioni

  • IMP 5'-nucleotidase activity Source: SGD
  • magnesium ion binding Source: InterPro

GO - Biological processi

  • inosine salvage Source: SGD
  • nicotinamide riboside biosynthetic process Source: SGD
  • nicotinic acid riboside biosynthetic process Source: SGD
  • nucleotide metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciYEAST:G3O-33672-MONOMER.
YEAST:MONOMER3O-10.

Names & Taxonomyi

Protein namesi
Recommended name:
IMP-specific 5'-nucleotidase 1 (EC:3.1.3.-)
Gene namesi
Name:ISN1
Ordered Locus Names:YOR155C
ORF Names:O3548
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR155C.
SGDiS000005681. ISN1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 450450IMP-specific 5'-nucleotidase 1PRO_0000084257Add
BLAST

Proteomic databases

MaxQBiQ99312.
PRIDEiQ99312.

PTM databases

iPTMnetiQ99312.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi34551. 16 interactions.
DIPiDIP-4027N.
IntActiQ99312. 5 interactions.
MINTiMINT-510621.

Structurei

3D structure databases

ProteinModelPortaliQ99312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ISN1 family.Curated

Phylogenomic databases

HOGENOMiHOG000200133.
KOiK18550.
OMAiIEWIKGL.
OrthoDBiEOG7FJH9C.

Family and domain databases

InterProiIPR023214. HAD-like_dom.
IPR009453. ISN1.
[Graphical view]
PfamiPF06437. ISN1. 1 hit.
[Graphical view]
PIRSFiPIRSF028836. ISN1. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.

Sequencei

Sequence statusi: Complete.

Q99312-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSRYRVEYH LKSHRKDEFI DWVKGLLASP FVLHAVSHEG DYNDDLATTQ
60 70 80 90 100
RVRSQYADIF KDIEGLIKDK IEFDSRNMSQ DEIEDGASSQ SLNILGQSRL
110 120 130 140 150
NLLVPSIGTF FTELPLEQAF LWEDSQRAIS ARRMVAPSFN DIRHILNTAQ
160 170 180 190 200
IFHFKKQENL HNGKVLRLVT FDGDVTLYED GGSLVYTNPV IPYILKLLRC
210 220 230 240 250
GINVGIVTAA GYDEAGTYEN RLKGLIVALH DSTDIPVSQK QNLTIMGGES
260 270 280 290 300
SYLFRYYEDP EEDNFGFRQI DKEEWLLPRM KAWSLEDVEK TLDFAERTLN
310 320 330 340 350
RLRKRLNLPS EISIIRKVRA VGIVPGERYD EASKRQVPVK LDREQLEEIV
360 370 380 390 400
LTLQNTLESF APSRRIQFSC FDGGSDVWCD IGGKDLGVRS LQQFYNPESP
410 420 430 440 450
IQPSETLHVG DQFAPVGSAN DFKARLAGCT LWIASPQETV NYLHRLLETD
Length:450
Mass (Da):51,330
Last modified:November 1, 1996 - v1
Checksum:iE0F3033FF2C18F07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55020 Genomic DNA. Translation: AAC49641.1.
Z75063 Genomic DNA. Translation: CAA99361.1.
BK006948 Genomic DNA. Translation: DAA10928.1.
PIRiS67043.
RefSeqiNP_014798.3. NM_001183574.3.

Genome annotation databases

EnsemblFungiiYOR155C; YOR155C; YOR155C.
GeneIDi854326.
KEGGisce:YOR155C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55020 Genomic DNA. Translation: AAC49641.1.
Z75063 Genomic DNA. Translation: CAA99361.1.
BK006948 Genomic DNA. Translation: DAA10928.1.
PIRiS67043.
RefSeqiNP_014798.3. NM_001183574.3.

3D structure databases

ProteinModelPortaliQ99312.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34551. 16 interactions.
DIPiDIP-4027N.
IntActiQ99312. 5 interactions.
MINTiMINT-510621.

PTM databases

iPTMnetiQ99312.

Proteomic databases

MaxQBiQ99312.
PRIDEiQ99312.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR155C; YOR155C; YOR155C.
GeneIDi854326.
KEGGisce:YOR155C.

Organism-specific databases

EuPathDBiFungiDB:YOR155C.
SGDiS000005681. ISN1.

Phylogenomic databases

HOGENOMiHOG000200133.
KOiK18550.
OMAiIEWIKGL.
OrthoDBiEOG7FJH9C.

Enzyme and pathway databases

BioCyciYEAST:G3O-33672-MONOMER.
YEAST:MONOMER3O-10.

Miscellaneous databases

PROiQ99312.

Family and domain databases

InterProiIPR023214. HAD-like_dom.
IPR009453. ISN1.
[Graphical view]
PfamiPF06437. ISN1. 1 hit.
[Graphical view]
PIRSFiPIRSF028836. ISN1. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 35.6 kb region on the right arm of Saccharomyces cerevisiae chromosome XV."
    Bordonne R., Camasses A., Madania A., Poch O., Tarassov I.A., Winsor B., Martin R.P.
    Yeast 13:73-83(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1678.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Purification and some properties of an IMP-specific 5'-nucleotidase from yeast."
    Itoh R.
    Biochem. J. 298:593-598(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  5. "The yeast ISN1 (YOR155c) gene encodes a new type of IMP-specific 5'-nucleotidase."
    Itoh R., Saint-Marc C., Chaignepain S., Katahira R., Schmitter J.-M., Daignan-Fornier B.
    BMC Biochem. 4:4-4(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, DOMAIN.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiISN1_YEAST
AccessioniPrimary (citable) accession number: Q99312
Secondary accession number(s): D6W2L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.