Q992I2 (SIGC_ARVS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sigma-C capsid protein

Alternative name(s):
Sigma-3 protein

Gene names

ORF Names:

ORF3

Organism

Avian reovirus (strain S1133) (ARV)

Taxonomic identifier

38171 [NCBI]

Taxonomic lineage

Viruses › dsRNA viruses › Reoviridae › Spinareovirinae › Orthoreovirus › Avian orthoreovirus

Virus host

Gallus gallus (Chicken) [TaxID: 9031]

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Structural protein responsible for cell attachment. Induces cell apoptosis. Ref.4
Subunit structure	Homotrimer.
Subcellular location	Virion Potential.
Domain	The C-terminus is important for the induction of cellular apoptosis.
Sequence similarities	Belongs to the orthoreovirus sigma C protein family.

Ontologies

Keywords
Biological process	Apoptosis Host-virus interaction Viral attachment to host cell Virus entry into host cell
Cellular component	Virion
Domain	Coiled coil
Molecular function	Capsid protein
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 326

326

Sigma-C capsid protein

PRO_0000222758

Regions

Coiled coil

60 – 80

21

Potential

Experimental info

Sequence conflict

57

1

T → S in AAC42113. Ref.1

Sequence conflict

97

1

S → P in AAC42113. Ref.1

Sequence conflict

162

1

F → L in AAC42113. Ref.1

Sequence conflict

193

1

A → E in AAC42113. Ref.1

Sequence conflict

204

1

A → E in AAC42113. Ref.1

Sequence conflict

221

1

H → Y in AAC42113. Ref.1

Secondary structure

1

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326

Helix Strand Turn

Details...

Helix

123 – 153

31

Turn

156 – 159

4

Beta strand

166 – 169

4

Beta strand

172 – 175

4

Turn

179 – 181

3

Beta strand

182 – 190

9

Beta strand

196 – 209

14

Beta strand

213 – 223

11

Beta strand

226 – 239

14

Beta strand

241 – 249

9

Helix

250 – 252

3

Beta strand

253 – 255

3

Helix

260 – 263

4

Helix

267 – 270

4

Beta strand

274 – 282

9

Beta strand

285 – 297

13

Beta strand

300 – 306

7

Beta strand

308 – 325

18

Sequences

	Sequence		Length	Mass (Da)
	Q992I2 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 7E2A0D3ADDEAC153	FASTA	326	34,856
	10 20 30 40 50 60 MAGLNPSQRR EVVSLILSLT SNVNISHGDL TPIYERLTNL EASTELLHRS ISDISTTVSN 70 80 90 100 110 120 ISANLQDMTH TLDDVTANLD GLRTTVTALQ DSVSILSTNV TDLTNRSSAH AAILSSLQTT 130 140 150 160 170 180 VDGNSTAISN LKSDISSNGL AITDLQDRVK SLESTASHGL SFSPPLSVAD GVVSLDMDPY 190 200 210 220 230 240 FCSQRVSLTS YSAEAQLMQF RWMARGTNGS SDTIDMTVNA HCHGRRTDYM MSSTGNLTVT 250 260 270 280 290 300 SNVVLLTFDL SDITHIPSDL ARLVPSAGFQ AASFPVDVSF TRDSATHAYQ AYGVYSSSRV 310 320 FTITFPTGGD GTANIRSLTV RTGIDT

References

[1]	"Cloning, sequencing and expression of the S1 gene of avian reovirus." Shapouri M.R., Kane M., Letarte M., Bergeron J., Arella M., Silim A. J. Gen. Virol. 76:1515-1520(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"The avian reovirus genome segment S1 is a functionally tricistronic gene that expresses one structural and two nonstructural proteins in infected cells." Bodelon G., Labrada L., Martinez-Costas J., Benavente J. Virology 290:181-191(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"Subunit composition and conformational stability of the oligomeric form of the avian reovirus cell-attachment protein sigmaC." Grande A., Costas C., Benavente J. J. Gen. Virol. 83:131-139(2002) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Avian reovirus sigmaC protein induces apoptosis in cultured cells." Shih W.L., Hsu H.W., Liao M.H., Lee L.H., Liu H.J. Virology 321:65-74(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L39002 Genomic RNA. Translation: AAC42113.1.
AF330703 Genomic RNA. Translation: AAK18188.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BSF	X-ray	2.10	A	151-326	[»]
2BT7	X-ray	2.35	A	157-326	[»]
2BT8	X-ray	3.00	A	157-326	[»]
2JJL	X-ray	2.30	A	117-326	[»]
2VRS	X-ray	1.75	A/B/C	117-326	[»]

ProteinModelPortal

Q992I2.

SMR

Q992I2. Positions 151-326.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR007662. Capsid_sigmaC_reovir.
[Graphical view]

Pfam

PF04582. Reo_sigmaC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q992I2.

Entry information

Entry name

SIGC_ARVS1

Accession

Primary (citable) accession number: Q992I2
Secondary accession number(s): Q84141

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 9, 2004
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program