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Protein

Broad-range acid phosphatase DET1

Gene

DET1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Metal-independent, broad-range acid phosphatase. Involved, either directly or indirectly, in the bidirectional transport of sterols between the endoplasmic reticulum and the plasma membrane.2 Publications

Miscellaneous

Present with 3170 molecules/cell in log phase SD medium.1 Publication

pH dependencei

Optimum pH is 4.5. Active from pH 4 to pH 5.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei32Tele-phosphohistidine intermediateBy similarity1
Binding sitei38SubstrateBy similarity1
Binding sitei108SubstrateBy similarity1
Active sitei126Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • acid phosphatase activity Source: SGD

GO - Biological processi

  • dephosphorylation Source: SGD
  • intracellular sterol transport Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processLipid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29661-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Broad-range acid phosphatase DET1 (EC:3.1.3.-)
Alternative name(s):
Decreased ergosterol transport protein 1
Gene namesi
Name:DET1
Ordered Locus Names:YDR051C
ORF Names:D4202
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR051C
SGDiS000002458 DET1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002444361 – 334Broad-range acid phosphatase DET1Add BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei248PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ99288
PaxDbiQ99288
PRIDEiQ99288

PTM databases

iPTMnetiQ99288

Interactioni

Protein-protein interaction databases

BioGridi32105, 50 interactors
DIPiDIP-1816N
IntActiQ99288, 9 interactors
MINTiQ99288
STRINGi4932.YDR051C

Structurei

3D structure databases

ProteinModelPortaliQ99288
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni44 – 45Substrate bindingBy similarity2
Regioni168 – 171Substrate bindingBy similarity4
Regioni195 – 205Substrate bindingBy similarityAdd BLAST11

Sequence similaritiesi

Belongs to the phosphoglycerate mutase family.Curated

Phylogenomic databases

HOGENOMiHOG000221683
InParanoidiQ99288
OMAiRLFCMRW
OrthoDBiEOG092C2YLJ

Family and domain databases

CDDicd07067 HP_PGM_like, 1 hit
InterProiView protein in InterPro
IPR013078 His_Pase_superF_clade-1
IPR029033 His_PPase_superfam
IPR001345 PG/BPGM_mutase_AS
PfamiView protein in Pfam
PF00300 His_Phos_1, 1 hit
SMARTiView protein in SMART
SM00855 PGAM, 1 hit
SUPFAMiSSF53254 SSF53254, 3 hits
PROSITEiView protein in PROSITE
PS00175 PG_MUTASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q99288-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCEENVHVSE DVAGSHGSFT NARPRLIVLI RHGESESNKN KEVNGYIPNH
60 70 80 90 100
LISLTKTGQI QARQAGIDLL RVLNVDDHNL VEDLAKKYIK DESSRRTLPL
110 120 130 140 150
KDYTRLSREK DTNIVFYTSP YRRARETLKG ILDVIDEYNE LNSGVRICED
160 170 180 190 200
MRYDPHGKQK HAFWPRGLNN TGGVYENNED NICEGKPGKC YLQYRVKDEP
210 220 230 240 250
RIREQDFGNF QKINSMQDVM KKRSTYGHFF FRFPHGESAA DVYDRVASFQ
260 270 280 290 300
ETLFRHFHDR QERRPRDVVV LVTHGIYSRV FLMKWFRWTY EEFESFTNVP
310 320 330
NGSVMVMELD ESINRYVLRT VLPKWTDCEG DLTT
Length:334
Mass (Da):39,172
Last modified:November 1, 1996 - v1
Checksum:iD1F68EA851DBEDD0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49209 Genomic DNA Translation: CAA89081.1
Z74347 Genomic DNA Translation: CAA98868.1
Z74348 Genomic DNA Translation: CAA98870.1
X84162 Genomic DNA Translation: CAA58968.1
BK006938 Genomic DNA Translation: DAA11898.1
PIRiS54036
RefSeqiNP_010336.3, NM_001180359.3

Genome annotation databases

EnsemblFungiiYDR051C; YDR051C; YDR051C
GeneIDi851621
KEGGisce:YDR051C

Similar proteinsi

Entry informationi

Entry nameiDET1_YEAST
AccessioniPrimary (citable) accession number: Q99288
Secondary accession number(s): D6VS38
, P87337, Q05314, Q7LHX4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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