Reviewed, UniProtKB/Swiss-Prot Q99288 (DET1_YEAST)

Names and origin

Protein names

Recommended name:
    Putative phosphoglycerate mutase DET1
    EC=5.4.2.1
Alternative name(s):
    Phosphoglyceromutase DET1
    Decreased ergosterol transport protein 1

Gene names

Name:	DET1
Ordered Locus Names:	YDR051C
ORF Names:	D4202

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved, either directly or indirectly, in the bidirectional transport of sterols between the endoplasmic reticulum and the plasma membrane. Ref.6
Catalytic activity	2-phospho-D-glycerate = 3-phospho-D-glycerate.
Subcellular location	Cytoplasm. Nucleus. Ref.3
Miscellaneous	Present with 3170 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the phosphoglycerate mutase family.

Ontologies

Keywords
Biological process	Lipid transport Transport
Cellular component	Cytoplasm Nucleus
Molecular function	Isomerase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intracellular sterol transport Ref.6 Inferred from mutant phenotype. Source: SGD metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Ref.3 Inferred from direct assay. Source: SGD nucleus Ref.3 Inferred from direct assay. Source: SGD
Molecular function	phosphoglycerate mutase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Putative phosphoglycerate mutase DET1		PRO_0000244436
Sites
	Active site	32	1	Tele-phosphohistidine intermediate By similarity
	Active site	274	1	By similarity
	Site	123	1	Interaction with carboxyl group of phosphoglycerates By similarity
Amino acid modifications
	Modified residue	248	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q99288-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D1F68EA851DBEDD0	FASTA	334	39,172
	10 20 30 40 50 60 MCEENVHVSE DVAGSHGSFT NARPRLIVLI RHGESESNKN KEVNGYIPNH LISLTKTGQI 70 80 90 100 110 120 QARQAGIDLL RVLNVDDHNL VEDLAKKYIK DESSRRTLPL KDYTRLSREK DTNIVFYTSP 130 140 150 160 170 180 YRRARETLKG ILDVIDEYNE LNSGVRICED MRYDPHGKQK HAFWPRGLNN TGGVYENNED 190 200 210 220 230 240 NICEGKPGKC YLQYRVKDEP RIREQDFGNF QKINSMQDVM KKRSTYGHFF FRFPHGESAA 250 260 270 280 290 300 DVYDRVASFQ ETLFRHFHDR QERRPRDVVV LVTHGIYSRV FLMKWFRWTY EEFESFTNVP 310 320 330 NGSVMVMELD ESINRYVLRT VLPKWTDCEG DLTT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Nucleotide sequence analysis of a 32,500 bp region of the right arm of Saccharomyces cerevisiae chromosome IV." Brandt P., Ramlow S., Otto B., Bloecker H. Yeast 12:85-90(1996) [PubMed: 8789263] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-154.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-248, MASS SPECTROMETRY.
[6]	"Tritium suicide selection identifies proteins involved in the uptake and intracellular transport of sterols in Saccharomyces cerevisiae." Sullivan D.P., Georgiev A., Menon A.K. Eukaryot. Cell 8:161-169(2009) [PubMed: 19060182] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49209 Genomic DNA. Translation: CAA89081.1. Z74347 Genomic DNA. Translation: CAA98868.1. Z74348 Genomic DNA. Translation: CAA98870.1. X84162 Genomic DNA. Translation: CAA58968.1.
PIR	S54036.
RefSeq	NP_010336.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1816N.
IntAct	Q99288. 10 interactions.
STRING	Q99288.
Proteomic databases
PeptideAtlas	Q99288.
Genome annotation databases
Ensembl	YDR051C; YDR051C; YDR051C; Saccharomyces cerevisiae. [Genome view]
GeneID	851621.
GenomeReviews	Gene locus YDR051C in contig Z71256_GR.
KEGG	sce:YDR051C.
NMPDR	fig|4932.3.peg.1081.
Organism-specific databases
CYGD	YDR051c.
SGD	S000002458. YDR051C.
Phylogenomic databases
HOGENOM	Q99288.
OMA	GESESNK.
Enzyme and pathway databases
BRENDA	5.4.2.1. 250.
Gene expression databases
ArrayExpress	Q99288.
Genevestigator	Q99288.
GermOnline	YDR051C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001345. PG/BPGM_mutase_AC. IPR013078. PG_mutase. [Graphical view]
Pfam	PF00300. PGAM. 1 hit. [Graphical view]
PROSITE	PS00175. PG_MUTASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	969154.

Entry information

Entry name

DET1_YEAST

Accession

Primary (citable) accession number: Q99288
Secondary accession number(s): P87337, Q05314, Q7LHX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)