Q99288 (DET1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative phosphoglycerate mutase DET1
EC=5.4.2.1

Alternative name(s):
Decreased ergosterol transport protein 1
Phosphoglyceromutase DET1

Gene names

Name:	DET1
Ordered Locus Names:	YDR051C
ORF Names:	D4202

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved, either directly or indirectly, in the bidirectional transport of sterols between the endoplasmic reticulum and the plasma membrane. Ref.7
Catalytic activity	2-phospho-D-glycerate = 3-phospho-D-glycerate.
Subcellular location	Cytoplasm. Nucleus Ref.4.
Miscellaneous	Present with 3170 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the phosphoglycerate mutase family.

Ontologies

Keywords
Biological process	Lipid transport Transport
Cellular component	Cytoplasm Nucleus
Molecular function	Isomerase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intracellular sterol transport Inferred from mutant phenotype Ref.7. Source: SGD
Cellular component	cytoplasm Inferred from direct assay Ref.4. Source: SGD nucleus Inferred from direct assay Ref.4. Source: SGD
Molecular function	acid phosphatase activity Inferred from direct assay. Source: SGD phosphoglycerate mutase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Putative phosphoglycerate mutase DET1		PRO_0000244436
Sites
	Active site	32	1	Tele-phosphohistidine intermediate By similarity
	Active site	274	1	By similarity
	Site	123	1	Interaction with carboxyl group of phosphoglycerates By similarity
Amino acid modifications
	Modified residue	248	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q99288 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D1F68EA851DBEDD0	FASTA	334	39,172
	10 20 30 40 50 60 MCEENVHVSE DVAGSHGSFT NARPRLIVLI RHGESESNKN KEVNGYIPNH LISLTKTGQI 70 80 90 100 110 120 QARQAGIDLL RVLNVDDHNL VEDLAKKYIK DESSRRTLPL KDYTRLSREK DTNIVFYTSP 130 140 150 160 170 180 YRRARETLKG ILDVIDEYNE LNSGVRICED MRYDPHGKQK HAFWPRGLNN TGGVYENNED 190 200 210 220 230 240 NICEGKPGKC YLQYRVKDEP RIREQDFGNF QKINSMQDVM KKRSTYGHFF FRFPHGESAA 250 260 270 280 290 300 DVYDRVASFQ ETLFRHFHDR QERRPRDVVV LVTHGIYSRV FLMKWFRWTY EEFESFTNVP 310 320 330 NGSVMVMELD ESINRYVLRT VLPKWTDCEG DLTT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Nucleotide sequence analysis of a 32,500 bp region of the right arm of Saccharomyces cerevisiae chromosome IV." Brandt P., Ramlow S., Otto B., Bloecker H. Yeast 12:85-90(1996) [PubMed: 8789263] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-154.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-248, MASS SPECTROMETRY.
[7]	"Tritium suicide selection identifies proteins involved in the uptake and intracellular transport of sterols in Saccharomyces cerevisiae." Sullivan D.P., Georgiev A., Menon A.K. Eukaryot. Cell 8:161-169(2009) [PubMed: 19060182] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49209 Genomic DNA. Translation: CAA89081.1. Z74347 Genomic DNA. Translation: CAA98868.1. Z74348 Genomic DNA. Translation: CAA98870.1. X84162 Genomic DNA. Translation: CAA58968.1. BK006938 Genomic DNA. Translation: DAA11898.1.
PIR	S54036.
RefSeq	NP_010336.1. NM_001180359.1.
3D structure databases
ProteinModelPortal	Q99288.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1816N.
IntAct	Q99288. 11 interactions.
MINT	MINT-395648.
STRING	Q99288.
Proteomic databases
PeptideAtlas	Q99288.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR051C; YDR051C; YDR051C.
GeneID	851621.
KEGG	sce:YDR051C.
NMPDR	fig|4932.3.peg.1081.
Organism-specific databases
CYGD	YDR051c.
SGD	S000002458. YDR051C.
Phylogenomic databases
eggNOG	fuNOG06865.
GeneTree	EFGT00050000005577.
HOGENOM	HBG649033.
OMA	EEFESFT.
OrthoDB	EOG4H1F4T.
Gene expression databases
ArrayExpress	Q99288.
Genevestigator	Q99288.
GermOnline	YDR051C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013078. His_Pase_superF_clade-1. IPR001345. PG/BPGM_mutase_AS. [Graphical view]
Pfam	PF00300. His_Phos_1. 1 hit. [Graphical view]
SMART	SM00855. PGAM. 1 hit. [Graphical view]
PROSITE	PS00175. PG_MUTASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	969154.

Entry information

Entry name

DET1_YEAST

Accession

Primary (citable) accession number: Q99288
Secondary accession number(s): D6VS38 , P87337, Q05314, Q7LHX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program