Reviewed,
UniProtKB/Swiss-Prot Q99288 (DET1_YEAST)
Last modified
November 3, 2009.
Version 61.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Putative phosphoglycerate mutase DET1 EC=5.4.2.1 Alternative name(s): Phosphoglyceromutase DET1 Decreased ergosterol transport protein 1 | ||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 334 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved, either directly or indirectly, in the bidirectional transport of sterols between the endoplasmic reticulum and the plasma membrane. Ref.6 |
| Catalytic activity | 2-phospho-D-glycerate = 3-phospho-D-glycerate. |
| Subcellular location | |
| Miscellaneous | Present with 3170 molecules/cell in log phase SD medium. Ref.4 |
| Sequence similarities | Belongs to the phosphoglycerate mutase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Lipid transport Transport |
| Cellular component | Cytoplasm Nucleus |
| Molecular function | Isomerase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | intracellular sterol transport Ref.6 Inferred from mutant phenotype. Source: SGD metabolic processInferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Ref.3 Inferred from direct assay. Source: SGD nucleus Ref.3Inferred from direct assay. Source: SGD |
| Molecular function | phosphoglycerate mutase activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 334 | 334 | Putative phosphoglycerate mutase DET1 | PRO_0000244436 | |||||
Sites | |||||||||
| Active site | 32 | 1 | Tele-phosphohistidine intermediate By similarity | ||||||
| Active site | 274 | 1 | By similarity | ||||||
| Site | 123 | 1 | Interaction with carboxyl group of phosphoglycerates By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 248 | 1 | Phosphoserine Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. Zaccaria P.Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [2] | "Nucleotide sequence analysis of a 32,500 bp region of the right arm of Saccharomyces cerevisiae chromosome IV." Brandt P., Ramlow S., Otto B., Bloecker H. Yeast 12:85-90(1996) [PubMed: 8789263] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-154. |
| [3] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [4] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [5] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-248, MASS SPECTROMETRY. |
| [6] | "Tritium suicide selection identifies proteins involved in the uptake and intracellular transport of sterols in Saccharomyces cerevisiae." Sullivan D.P., Georgiev A., Menon A.K. Eukaryot. Cell 8:161-169(2009) [PubMed: 19060182] [Abstract] Cited for: FUNCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| Z49209 Genomic DNA. Translation: CAA89081.1. Z74347 Genomic DNA. Translation: CAA98868.1. Z74348 Genomic DNA. Translation: CAA98870.1. X84162 Genomic DNA. Translation: CAA58968.1. | |
| PIR | S54036. |
| RefSeq | NP_010336.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:1816N. |
| IntAct | Q99288. 10 interactions. |
| STRING | Q99288. |
Proteomic databases | |
| PeptideAtlas | Q99288. |
Genome annotation databases | |
| Ensembl | YDR051C; YDR051C; YDR051C; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 851621. |
| GenomeReviews | Gene locus YDR051C in contig Z71256_GR. |
| KEGG | sce:YDR051C. |
| NMPDR | fig|4932.3.peg.1081. |
Organism-specific databases | |
| CYGD | YDR051c. |
| SGD | S000002458. YDR051C. |
Phylogenomic databases | |
| HOGENOM | Q99288. |
| OMA | GESESNK. |
Enzyme and pathway databases | |
| BRENDA | 5.4.2.1. 250. |
Gene expression databases | |
| ArrayExpress | Q99288. |
| Genevestigator | Q99288. |
| GermOnline | YDR051C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR001345. PG/BPGM_mutase_AC. IPR013078. PG_mutase. [Graphical view] |
| Pfam | PF00300. PGAM. 1 hit. [Graphical view] |
| PROSITE | PS00175. PG_MUTASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 969154. |
Entry information
| Entry name | DET1_YEAST | ||||||||
| Accession | Primary (citable) accession number: Q99288 Secondary accession number(s): P87337, Q05314, Q7LHX4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |

Clusters with


