Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q99288 (DET1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative phosphoglycerate mutase DET1

EC=5.4.2.1
Alternative name(s):
Decreased ergosterol transport protein 1
Phosphoglyceromutase DET1
Gene names
Name:DET1
Ordered Locus Names:YDR051C
ORF Names:D4202
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved, either directly or indirectly, in the bidirectional transport of sterols between the endoplasmic reticulum and the plasma membrane. Ref.7

Catalytic activity

2-phospho-D-glycerate = 3-phospho-D-glycerate.

Subcellular location

Cytoplasm. Nucleus Ref.4.

Miscellaneous

Present with 3170 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the phosphoglycerate mutase family.

Ontologies

Keywords
   Biological processLipid transport
Transport
   Cellular componentCytoplasm
Nucleus
   Molecular functionIsomerase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processintracellular sterol transport

Inferred from mutant phenotype Ref.7. Source: SGD

   Cellular componentcytoplasm

Inferred from direct assay Ref.4. Source: SGD

nucleus

Inferred from direct assay Ref.4. Source: SGD

   Molecular functionacid phosphatase activity

Inferred from direct assay. Source: SGD

phosphoglycerate mutase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Putative phosphoglycerate mutase DET1
PRO_0000244436

Sites

Active site321Tele-phosphohistidine intermediate By similarity
Active site2741 By similarity
Site1231Interaction with carboxyl group of phosphoglycerates By similarity

Amino acid modifications

Modified residue2481Phosphoserine Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q99288 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D1F68EA851DBEDD0

FASTA33439,172
        10         20         30         40         50         60 
MCEENVHVSE DVAGSHGSFT NARPRLIVLI RHGESESNKN KEVNGYIPNH LISLTKTGQI 

        70         80         90        100        110        120 
QARQAGIDLL RVLNVDDHNL VEDLAKKYIK DESSRRTLPL KDYTRLSREK DTNIVFYTSP 

       130        140        150        160        170        180 
YRRARETLKG ILDVIDEYNE LNSGVRICED MRYDPHGKQK HAFWPRGLNN TGGVYENNED 

       190        200        210        220        230        240 
NICEGKPGKC YLQYRVKDEP RIREQDFGNF QKINSMQDVM KKRSTYGHFF FRFPHGESAA 

       250        260        270        280        290        300 
DVYDRVASFQ ETLFRHFHDR QERRPRDVVV LVTHGIYSRV FLMKWFRWTY EEFESFTNVP 

       310        320        330 
NGSVMVMELD ESINRYVLRT VLPKWTDCEG DLTT 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed: 9169867] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Nucleotide sequence analysis of a 32,500 bp region of the right arm of Saccharomyces cerevisiae chromosome IV."
Brandt P., Ramlow S., Otto B., Bloecker H.
Yeast 12:85-90(1996) [PubMed: 8789263] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-154.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed: 14562095] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-248, MASS SPECTROMETRY.
[7]"Tritium suicide selection identifies proteins involved in the uptake and intracellular transport of sterols in Saccharomyces cerevisiae."
Sullivan D.P., Georgiev A., Menon A.K.
Eukaryot. Cell 8:161-169(2009) [PubMed: 19060182] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49209 Genomic DNA. Translation: CAA89081.1.
Z74347 Genomic DNA. Translation: CAA98868.1.
Z74348 Genomic DNA. Translation: CAA98870.1.
X84162 Genomic DNA. Translation: CAA58968.1.
BK006938 Genomic DNA. Translation: DAA11898.1.
PIRS54036.
RefSeqNP_010336.1. NM_001180359.1.

3D structure databases

ProteinModelPortalQ99288.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1816N.
IntActQ99288. 11 interactions.
MINTMINT-395648.
STRINGQ99288.

Proteomic databases

PeptideAtlasQ99288.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR051C; YDR051C; YDR051C.
GeneID851621.
KEGGsce:YDR051C.
NMPDRfig|4932.3.peg.1081.

Organism-specific databases

CYGDYDR051c.
SGDS000002458. YDR051C.

Phylogenomic databases

eggNOGfuNOG06865.
GeneTreeEFGT00050000005577.
HOGENOMHBG649033.
OMAEEFESFT.
OrthoDBEOG4H1F4T.

Gene expression databases

ArrayExpressQ99288.
GenevestigatorQ99288.
GermOnlineYDR051C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR013078. His_Pase_superF_clade-1.
IPR001345. PG/BPGM_mutase_AS.
[Graphical view]
PfamPF00300. His_Phos_1. 1 hit.
[Graphical view]
SMARTSM00855. PGAM. 1 hit.
[Graphical view]
PROSITEPS00175. PG_MUTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio969154.

Entry information

Entry nameDET1_YEAST
AccessionPrimary (citable) accession number: Q99288
Secondary accession number(s): D6VS38 expand/collapse secondary AC list , P87337, Q05314, Q7LHX4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families