Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein SEY1

Gene

SEY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cooperates with the reticulon proteins RTN1 and RTN2 and the tubule-shaping DP1 family protein YOP1 to generate and maintain the structure of the tubular endoplasmic reticulum network. Has GTPase activity, which is required for its function in ER organization.UniRule annotation2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi44 – 51GTPUniRule annotation8

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-HAMAP

GO - Biological processi

  • endoplasmic reticulum inheritance Source: UniProtKB
  • endoplasmic reticulum membrane fusion Source: SGD
  • endoplasmic reticulum organization Source: UniProtKB
  • membrane organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Stress response

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33681-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SEY1UniRule annotation (EC:3.6.5.-UniRule annotation)
Alternative name(s):
Synthetic enhancer of YOP1 proteinUniRule annotation
Gene namesi
Name:SEY1UniRule annotation
Ordered Locus Names:YOR165W
ORF Names:O3590
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR165W.
SGDiS000005691. SEY1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 681CytoplasmicUniRule annotationAdd BLAST681
Transmembranei682 – 702HelicalUniRule annotationAdd BLAST21
Topological domaini703 – 705LumenalUniRule annotation3
Transmembranei706 – 726HelicalUniRule annotationAdd BLAST21
Topological domaini727 – 776CytoplasmicUniRule annotationAdd BLAST50

GO - Cellular componenti

  • cortical endoplasmic reticulum Source: SGD
  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi50K → A: Abolishes GTP binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001551401 – 776Protein SEY1Add BLAST776

Proteomic databases

MaxQBiQ99287.
PRIDEiQ99287.

PTM databases

iPTMnetiQ99287.

Expressioni

Inductioni

By salt stress.1 Publication

Interactioni

Subunit structurei

Interacts with RTN1 and YOP1; GTP binding is not required for these interactions.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RTN1Q049472EBI-37523,EBI-38020
YOP1Q124022EBI-37523,EBI-37092

Protein-protein interaction databases

BioGridi34561. 73 interactors.
DIPiDIP-49373N.
IntActiQ99287. 10 interactors.
MINTiMINT-2493571.

Structurei

3D structure databases

ProteinModelPortaliQ99287.
SMRiQ99287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 263GB1/RHD3-type GAdd BLAST230

Sequence similaritiesi

Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3-type GTPase family. RHD3 subfamily.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000206561.
InParanoidiQ99287.
OMAiIRILAWR.
OrthoDBiEOG092C0ULX.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_03109. Sey1. 1 hit.
InterProiIPR030386. G_GB1_RHD3_dom.
IPR027417. P-loop_NTPase.
IPR008803. RHD3/Sey1.
[Graphical view]
PfamiPF05879. RHD3. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51715. G_GB1_RHD3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99287-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRPAIQLI DEEKEFHQSA LQYFQQCIGN RDVGLDYHVI SVFGSQSSGK
60 70 80 90 100
STLLNVLFNT NFDTMDAQVK RQQTTKGIWL AHTKQVNTTI EIDNDRPDIF
110 120 130 140 150
VLDVEGSDGS ERGEDQDFER KAALFAIAVS EVLIVNMWEQ QIGLYQGNNM
160 170 180 190 200
ALLKTVFEVN LSLFGKNDND HKVLLLFVIR DHVGVTPLSS LSDSVTRELE
210 220 230 240 250
KIWTELSKPA GCEGSSLYDY FDLKFVGLAH KLLQEDKFTQ DVKKLGDSFV
260 270 280 290 300
MKGTENYYFK PQYHHRLPLD GWTMYAENCW DQIERNKDLD LPTQQILVAR
310 320 330 340 350
FKTEEISNEA LEEFISKYDE SIAPLKGNLG SLTSQLVKLK EECLTKYDEQ
360 370 380 390 400
ASRYARNVYM EKREALNTKL NSHISGTINE FLESLMEKLW DDLKLEVSSR
410 420 430 440 450
DKATTSFVES VAAGKSKIEK EFNESMETFK KLGLLISNEE ITCKFSDDIE
460 470 480 490 500
ERIKQLRDAE LKAKIGRIKK NLVPELKDHV IHLLSHPSKK VWDDIMNDFE
510 520 530 540 550
STIKDNISAY QVEKDKYDFK IGLSESENAK IYKNIRILAW RTLDTTVHDY
560 570 580 590 600
LKIDTIVSIL RDRFEDVFRY DAEGSPRLWK TEEEIDGAFR VAKEHALEVF
610 620 630 640 650
EVLSLAVTSD NVEIIPDVPM AEEESGEDNE IYRDNEGVFH SRRFAHILTE
660 670 680 690 700
LQKENVLDQF RRQINITVLD SKRSIITTRT HIPPWIYVLL AVLGWNEFVA
710 720 730 740 750
VIRNPLFVTL TLILGATFFV IHKFGLWGPV VNVVQSAVGE TRTAIKDKLR
760 770
QFVVEDHEVK ESFEMKDFSK NEQKEK
Length:776
Mass (Da):89,432
Last modified:November 1, 1996 - v1
Checksum:iDEC0C9EC3AB172DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55021 Genomic DNA. Translation: AAB47412.1.
Z75073 Genomic DNA. Translation: CAA99371.1.
BK006948 Genomic DNA. Translation: DAA10939.1.
PIRiS67053.
RefSeqiNP_014808.1. NM_001183584.1.

Genome annotation databases

EnsemblFungiiYOR165W; YOR165W; YOR165W.
GeneIDi854336.
KEGGisce:YOR165W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55021 Genomic DNA. Translation: AAB47412.1.
Z75073 Genomic DNA. Translation: CAA99371.1.
BK006948 Genomic DNA. Translation: DAA10939.1.
PIRiS67053.
RefSeqiNP_014808.1. NM_001183584.1.

3D structure databases

ProteinModelPortaliQ99287.
SMRiQ99287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34561. 73 interactors.
DIPiDIP-49373N.
IntActiQ99287. 10 interactors.
MINTiMINT-2493571.

PTM databases

iPTMnetiQ99287.

Proteomic databases

MaxQBiQ99287.
PRIDEiQ99287.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR165W; YOR165W; YOR165W.
GeneIDi854336.
KEGGisce:YOR165W.

Organism-specific databases

EuPathDBiFungiDB:YOR165W.
SGDiS000005691. SEY1.

Phylogenomic databases

HOGENOMiHOG000206561.
InParanoidiQ99287.
OMAiIRILAWR.
OrthoDBiEOG092C0ULX.

Enzyme and pathway databases

BioCyciYEAST:G3O-33681-MONOMER.

Miscellaneous databases

PROiQ99287.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_03109. Sey1. 1 hit.
InterProiIPR030386. G_GB1_RHD3_dom.
IPR027417. P-loop_NTPase.
IPR008803. RHD3/Sey1.
[Graphical view]
PfamiPF05879. RHD3. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51715. G_GB1_RHD3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSEY1_YEAST
AccessioniPrimary (citable) accession number: Q99287
Secondary accession number(s): D6W2M3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2265 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.