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Protein

mRNA export factor MEX67

Gene

MEX67

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the export of mRNA from the nucleus to the cytoplasm.PROSITE-ProRule annotation1 Publication

GO - Molecular functioni

  • RNA binding Source: SGD

GO - Biological processi

  • mRNA export from nucleus Source: SGD
  • poly(A)+ mRNA export from nucleus Source: GO_Central
  • ribosomal large subunit export from nucleus Source: SGD
  • ribosomal small subunit export from nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34065-MONOMER.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.
3.A.22.1.1. the transcription-coupled trex/tap nuclear mrna export complex (trex) family.

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA export factor MEX67
Gene namesi
Name:MEX67
Ordered Locus Names:YPL169C
ORF Names:P2520
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL169C.
SGDiS000006090. MEX67.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Localizes at both the nuclear and cytoplasmic site of the pores. Shuttles between the nucleus and the cytoplasm.

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nuclear RNA export factor complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi400H → Y: Impairs association with the nuclear pores and interaction with MTR2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002205422 – 599mRNA export factor MEX67Add BLAST598

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ99257.
PRIDEiQ99257.
TopDownProteomicsiQ99257.

PTM databases

iPTMnetiQ99257.

Interactioni

Subunit structurei

Interacts with nucleoporin complex NUP84 and MTR2. Interacts with MIP6.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NUP116Q026305EBI-11642,EBI-11703

Protein-protein interaction databases

BioGridi36014. 147 interactors.
DIPiDIP-3984N.
IntActiQ99257. 18 interactors.
MINTiMINT-441559.

Structurei

Secondary structure

1599
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi24 – 29Combined sources6
Helixi35 – 45Combined sources11
Beta strandi51 – 54Combined sources4
Beta strandi59 – 63Combined sources5
Helixi67 – 75Combined sources9
Beta strandi82 – 85Combined sources4
Beta strandi87 – 91Combined sources5
Helixi102 – 114Combined sources13
Turni117 – 120Combined sources4
Beta strandi121 – 123Combined sources3
Helixi127 – 129Combined sources3
Helixi131 – 136Combined sources6
Beta strandi140 – 143Combined sources4
Helixi148 – 159Combined sources12
Beta strandi165 – 168Combined sources4
Helixi177 – 182Combined sources6
Helixi183 – 186Combined sources4
Beta strandi192 – 194Combined sources3
Helixi205 – 209Combined sources5
Beta strandi218 – 220Combined sources3
Helixi225 – 228Combined sources4
Helixi232 – 239Combined sources8
Helixi254 – 260Combined sources7
Beta strandi269 – 274Combined sources6
Helixi279 – 294Combined sources16
Helixi296 – 302Combined sources7
Beta strandi303 – 313Combined sources11
Turni332 – 337Combined sources6
Turni341 – 343Combined sources3
Helixi347 – 353Combined sources7
Helixi357 – 367Combined sources11
Beta strandi371 – 373Combined sources3
Turni375 – 377Combined sources3
Helixi379 – 381Combined sources3
Beta strandi383 – 389Combined sources7
Helixi390 – 392Combined sources3
Beta strandi394 – 405Combined sources12
Beta strandi441 – 454Combined sources14
Beta strandi457 – 469Combined sources13
Helixi546 – 559Combined sources14
Helixi563 – 572Combined sources10
Turni573 – 575Combined sources3
Helixi578 – 586Combined sources9
Turni588 – 590Combined sources3
Helixi593 – 596Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OF5X-ray2.80A268-484[»]
2JP7NMR-A543-599[»]
2KHHNMR-A543-599[»]
4WWUX-ray3.30A/B/D/E/G/H/J/K1-487[»]
ProteinModelPortaliQ99257.
SMRiQ99257.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99257.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati163 – 184LRR 1Add BLAST22
Repeati189 – 210LRR 2Add BLAST22
Domaini224 – 262LRRCTAdd BLAST39
Domaini280 – 467NTF2PROSITE-ProRule annotationAdd BLAST188
Domaini546 – 599TAP-CPROSITE-ProRule annotationAdd BLAST54

Domaini

The leucine-rich repeats and the NTF2-domain are essential for the export of mRNA from the nucleus.By similarity
The NTF2 domain heterodimerizes with MTR2. The formation of this heterodimer is essential for mRNA export and binds to all of the nucleoporin-FG-repeats.
The RNA-binding domain is conserved in most NXF proteins but may be absent in yeasts.

Sequence similaritiesi

Belongs to the NXF family.Curated
Contains 2 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 NTF2 domain.PROSITE-ProRule annotation
Contains 1 TAP-C domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

HOGENOMiHOG000248843.
InParanoidiQ99257.
KOiK14284.
OMAiNHLPARM.
OrthoDBiEOG092C3PB8.

Family and domain databases

CDDicd14342. UBA_TAP-C. 1 hit.
Gene3Di3.10.450.50. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR032710. NTF2-like_dom.
IPR018222. Nuclear_transport_factor_2_euk.
IPR030217. NXF_fam.
IPR005637. TAP_C_dom.
IPR009060. UBA-like.
[Graphical view]
PANTHERiPTHR10662. PTHR10662. 2 hits.
PfamiPF03943. TAP_C. 1 hit.
[Graphical view]
SMARTiSM00804. TAP_C. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF52058. SSF52058. 1 hit.
SSF54427. SSF54427. 1 hit.
PROSITEiPS51450. LRR. 3 hits.
PS50177. NTF2_DOMAIN. 1 hit.
PS51281. TAP_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99257-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGFHNVGNI NMMAQQQMQQ NRIKISVRNW QNATMNDLIN FISRNARVAV
60 70 80 90 100
YDAHVEGPLV IGYVNSKAEA ESLMKWNGVR FAGSNLKFEL LDNNGASAGT
110 120 130 140 150
SDTISFLRGV LLKRYDPQTK LLNLGALHSD PELIQKGVFS SISTQSKMFP
160 170 180 190 200
AMMKLASTEK SLIVESVNLA DNQLKDISAI STLAQTFPNL KNLCLANNQI
210 220 230 240 250
FRFRSLEVWK NKFKDLRELL MTNNPITTDK LYRTEMLRLF PKLVVLDNVI
260 270 280 290 300
VRDEQKLQTV YSLPMKIQQF FFENDALGQS STDFATNFLN LWDNNREQLL
310 320 330 340 350
NLYSPQSQFS VSVDSTIPPS TVTDSDQTPA FGYYMSSSRN ISKVSSEKSI
360 370 380 390 400
QQRLSIGQES INSIFKTLPK TKHHLQEQPN EYSMETISYP QINGFVITLH
410 420 430 440 450
GFFEETGKPE LESNKKTGKN NYQKNRRYNH GYNSTSNNKL SKKSFDRTWV
460 470 480 490 500
IVPMNNSVII ASDLLTVRAY STGAWKTASI AIAQPPQQQA SVLPQVASMN
510 520 530 540 550
PNITTPPQPQ PSVVPGGMSI PGAPQGAMVM APTLQLPPDV QSRLNPVQLE
560 570 580 590
LLNKLHLETK LNAEYTFMLA EQSNWNYEVA IKGFQSSMNG IPREAFVQF
Length:599
Mass (Da):67,352
Last modified:November 1, 1996 - v1
Checksum:i6CE55EEC6FA40B40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96770 Genomic DNA. Translation: CAA65552.1.
Z73525 Genomic DNA. Translation: CAA97875.1.
BK006949 Genomic DNA. Translation: DAA11265.1.
PIRiS65180.
RefSeqiNP_015156.1. NM_001183983.1.

Genome annotation databases

EnsemblFungiiYPL169C; YPL169C; YPL169C.
GeneIDi855934.
KEGGisce:YPL169C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96770 Genomic DNA. Translation: CAA65552.1.
Z73525 Genomic DNA. Translation: CAA97875.1.
BK006949 Genomic DNA. Translation: DAA11265.1.
PIRiS65180.
RefSeqiNP_015156.1. NM_001183983.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OF5X-ray2.80A268-484[»]
2JP7NMR-A543-599[»]
2KHHNMR-A543-599[»]
4WWUX-ray3.30A/B/D/E/G/H/J/K1-487[»]
ProteinModelPortaliQ99257.
SMRiQ99257.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36014. 147 interactors.
DIPiDIP-3984N.
IntActiQ99257. 18 interactors.
MINTiMINT-441559.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.
3.A.22.1.1. the transcription-coupled trex/tap nuclear mrna export complex (trex) family.

PTM databases

iPTMnetiQ99257.

Proteomic databases

MaxQBiQ99257.
PRIDEiQ99257.
TopDownProteomicsiQ99257.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL169C; YPL169C; YPL169C.
GeneIDi855934.
KEGGisce:YPL169C.

Organism-specific databases

EuPathDBiFungiDB:YPL169C.
SGDiS000006090. MEX67.

Phylogenomic databases

HOGENOMiHOG000248843.
InParanoidiQ99257.
KOiK14284.
OMAiNHLPARM.
OrthoDBiEOG092C3PB8.

Enzyme and pathway databases

BioCyciYEAST:G3O-34065-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ99257.
PROiQ99257.

Family and domain databases

CDDicd14342. UBA_TAP-C. 1 hit.
Gene3Di3.10.450.50. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR032710. NTF2-like_dom.
IPR018222. Nuclear_transport_factor_2_euk.
IPR030217. NXF_fam.
IPR005637. TAP_C_dom.
IPR009060. UBA-like.
[Graphical view]
PANTHERiPTHR10662. PTHR10662. 2 hits.
PfamiPF03943. TAP_C. 1 hit.
[Graphical view]
SMARTiSM00804. TAP_C. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF52058. SSF52058. 1 hit.
SSF54427. SSF54427. 1 hit.
PROSITEiPS51450. LRR. 3 hits.
PS50177. NTF2_DOMAIN. 1 hit.
PS51281. TAP_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMEX67_YEAST
AccessioniPrimary (citable) accession number: Q99257
Secondary accession number(s): D6W3J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2830 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.