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Protein

mRNA export factor MEX67

Gene

MEX67

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the export of mRNA from the nucleus to the cytoplasm.PROSITE-ProRule annotation1 Publication

GO - Molecular functioni

  • RNA binding Source: SGD

GO - Biological processi

  • mRNA export from nucleus Source: SGD
  • ribosomal large subunit export from nucleus Source: SGD
  • ribosomal small subunit export from nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34065-MONOMER.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.
3.A.22.1.1. the transcription-coupled trex/tap nuclear mrna export complex (trex) family.

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA export factor MEX67
Gene namesi
Name:MEX67
Ordered Locus Names:YPL169C
ORF Names:P2520
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL169C.
SGDiS000006090. MEX67.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Localizes at both the nuclear and cytoplasmic site of the pores. Shuttles between the nucleus and the cytoplasm.

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nuclear RNA export factor complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi400 – 4001H → Y: Impairs association with the nuclear pores and interaction with MTR2. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 599598mRNA export factor MEX67PRO_0000220542Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ99257.
PeptideAtlasiQ99257.
TopDownProteomicsiQ99257.

PTM databases

iPTMnetiQ99257.

Interactioni

Subunit structurei

Interacts with nucleoporin complex NUP84 and MTR2. Interacts with MIP6.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NUP116Q026305EBI-11642,EBI-11703

Protein-protein interaction databases

BioGridi36014. 147 interactions.
DIPiDIP-3984N.
IntActiQ99257. 18 interactions.
MINTiMINT-441559.

Structurei

Secondary structure

1
599
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi24 – 296Combined sources
Helixi35 – 4511Combined sources
Beta strandi51 – 544Combined sources
Beta strandi59 – 635Combined sources
Helixi67 – 759Combined sources
Beta strandi82 – 854Combined sources
Beta strandi87 – 915Combined sources
Helixi102 – 11413Combined sources
Turni117 – 1204Combined sources
Beta strandi121 – 1233Combined sources
Helixi127 – 1293Combined sources
Helixi131 – 1366Combined sources
Beta strandi140 – 1434Combined sources
Helixi148 – 15912Combined sources
Beta strandi165 – 1684Combined sources
Helixi177 – 1826Combined sources
Helixi183 – 1864Combined sources
Beta strandi192 – 1943Combined sources
Helixi205 – 2095Combined sources
Beta strandi218 – 2203Combined sources
Helixi225 – 2284Combined sources
Helixi232 – 2398Combined sources
Helixi254 – 2607Combined sources
Beta strandi269 – 2746Combined sources
Helixi279 – 29416Combined sources
Helixi296 – 3027Combined sources
Beta strandi303 – 31311Combined sources
Turni332 – 3376Combined sources
Turni341 – 3433Combined sources
Helixi347 – 3537Combined sources
Helixi357 – 36711Combined sources
Beta strandi371 – 3733Combined sources
Turni375 – 3773Combined sources
Helixi379 – 3813Combined sources
Beta strandi383 – 3897Combined sources
Helixi390 – 3923Combined sources
Beta strandi394 – 40512Combined sources
Beta strandi441 – 45414Combined sources
Beta strandi457 – 46913Combined sources
Helixi546 – 55914Combined sources
Helixi563 – 57210Combined sources
Turni573 – 5753Combined sources
Helixi578 – 5869Combined sources
Turni588 – 5903Combined sources
Helixi593 – 5964Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OF5X-ray2.80A268-484[»]
2JP7NMR-A543-599[»]
2KHHNMR-A543-599[»]
4WWUX-ray3.30A/B/D/E/G/H/J/K1-487[»]
ProteinModelPortaliQ99257.
SMRiQ99257. Positions 14-477, 543-599.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99257.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati163 – 18422LRR 1Add
BLAST
Repeati189 – 21022LRR 2Add
BLAST
Domaini224 – 26239LRRCTAdd
BLAST
Domaini280 – 467188NTF2PROSITE-ProRule annotationAdd
BLAST
Domaini546 – 59954TAP-CPROSITE-ProRule annotationAdd
BLAST

Domaini

The leucine-rich repeats and the NTF2-domain are essential for the export of mRNA from the nucleus.By similarity
The NTF2 domain heterodimerizes with MTR2. The formation of this heterodimer is essential for mRNA export and binds to all of the nucleoporin-FG-repeats.
The RNA-binding domain is conserved in most NXF proteins but may be absent in yeasts.

Sequence similaritiesi

Belongs to the NXF family.Curated
Contains 2 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 NTF2 domain.PROSITE-ProRule annotation
Contains 1 TAP-C domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

HOGENOMiHOG000248843.
InParanoidiQ99257.
KOiK14284.
OMAiKYIMDCH.
OrthoDBiEOG7G7M0Q.

Family and domain databases

Gene3Di3.10.450.50. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR032710. NTF2-like_dom.
IPR018222. Nuclear_transport_factor_2_euk.
IPR030217. NXF_fam.
IPR005637. TAP_C_dom.
IPR009060. UBA-like.
[Graphical view]
PANTHERiPTHR10662. PTHR10662. 2 hits.
PfamiPF03943. TAP_C. 1 hit.
[Graphical view]
SMARTiSM00804. TAP_C. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF52058. SSF52058. 1 hit.
SSF54427. SSF54427. 1 hit.
PROSITEiPS51450. LRR. 3 hits.
PS50177. NTF2_DOMAIN. 1 hit.
PS51281. TAP_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99257-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGFHNVGNI NMMAQQQMQQ NRIKISVRNW QNATMNDLIN FISRNARVAV
60 70 80 90 100
YDAHVEGPLV IGYVNSKAEA ESLMKWNGVR FAGSNLKFEL LDNNGASAGT
110 120 130 140 150
SDTISFLRGV LLKRYDPQTK LLNLGALHSD PELIQKGVFS SISTQSKMFP
160 170 180 190 200
AMMKLASTEK SLIVESVNLA DNQLKDISAI STLAQTFPNL KNLCLANNQI
210 220 230 240 250
FRFRSLEVWK NKFKDLRELL MTNNPITTDK LYRTEMLRLF PKLVVLDNVI
260 270 280 290 300
VRDEQKLQTV YSLPMKIQQF FFENDALGQS STDFATNFLN LWDNNREQLL
310 320 330 340 350
NLYSPQSQFS VSVDSTIPPS TVTDSDQTPA FGYYMSSSRN ISKVSSEKSI
360 370 380 390 400
QQRLSIGQES INSIFKTLPK TKHHLQEQPN EYSMETISYP QINGFVITLH
410 420 430 440 450
GFFEETGKPE LESNKKTGKN NYQKNRRYNH GYNSTSNNKL SKKSFDRTWV
460 470 480 490 500
IVPMNNSVII ASDLLTVRAY STGAWKTASI AIAQPPQQQA SVLPQVASMN
510 520 530 540 550
PNITTPPQPQ PSVVPGGMSI PGAPQGAMVM APTLQLPPDV QSRLNPVQLE
560 570 580 590
LLNKLHLETK LNAEYTFMLA EQSNWNYEVA IKGFQSSMNG IPREAFVQF
Length:599
Mass (Da):67,352
Last modified:November 1, 1996 - v1
Checksum:i6CE55EEC6FA40B40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96770 Genomic DNA. Translation: CAA65552.1.
Z73525 Genomic DNA. Translation: CAA97875.1.
BK006949 Genomic DNA. Translation: DAA11265.1.
PIRiS65180.
RefSeqiNP_015156.1. NM_001183983.1.

Genome annotation databases

EnsemblFungiiYPL169C; YPL169C; YPL169C.
GeneIDi855934.
KEGGisce:YPL169C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96770 Genomic DNA. Translation: CAA65552.1.
Z73525 Genomic DNA. Translation: CAA97875.1.
BK006949 Genomic DNA. Translation: DAA11265.1.
PIRiS65180.
RefSeqiNP_015156.1. NM_001183983.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OF5X-ray2.80A268-484[»]
2JP7NMR-A543-599[»]
2KHHNMR-A543-599[»]
4WWUX-ray3.30A/B/D/E/G/H/J/K1-487[»]
ProteinModelPortaliQ99257.
SMRiQ99257. Positions 14-477, 543-599.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36014. 147 interactions.
DIPiDIP-3984N.
IntActiQ99257. 18 interactions.
MINTiMINT-441559.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.
3.A.22.1.1. the transcription-coupled trex/tap nuclear mrna export complex (trex) family.

PTM databases

iPTMnetiQ99257.

Proteomic databases

MaxQBiQ99257.
PeptideAtlasiQ99257.
TopDownProteomicsiQ99257.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL169C; YPL169C; YPL169C.
GeneIDi855934.
KEGGisce:YPL169C.

Organism-specific databases

EuPathDBiFungiDB:YPL169C.
SGDiS000006090. MEX67.

Phylogenomic databases

HOGENOMiHOG000248843.
InParanoidiQ99257.
KOiK14284.
OMAiKYIMDCH.
OrthoDBiEOG7G7M0Q.

Enzyme and pathway databases

BioCyciYEAST:G3O-34065-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ99257.
NextBioi980681.
PROiQ99257.

Family and domain databases

Gene3Di3.10.450.50. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR032710. NTF2-like_dom.
IPR018222. Nuclear_transport_factor_2_euk.
IPR030217. NXF_fam.
IPR005637. TAP_C_dom.
IPR009060. UBA-like.
[Graphical view]
PANTHERiPTHR10662. PTHR10662. 2 hits.
PfamiPF03943. TAP_C. 1 hit.
[Graphical view]
SMARTiSM00804. TAP_C. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF52058. SSF52058. 1 hit.
SSF54427. SSF54427. 1 hit.
PROSITEiPS51450. LRR. 3 hits.
PS50177. NTF2_DOMAIN. 1 hit.
PS51281. TAP_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators."
    Purnelle B., Coster F., Goffeau A.
    Yeast 12:1483-1492(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores."
    Segref A., Sharma K., Doye V., Hellwig A., Huber J., Luehrmann R., Hurt E.
    EMBO J. 16:3256-3271(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MIP6.
  5. "Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export."
    Straesser K., Bassler J., Hurt E.C.
    J. Cell Biol. 150:695-706(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS OF HIS-400.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Structural similarity in the absence of sequence homology of the messenger RNA export factors Mtr2 and p15."
    Fribourg S., Conti E.
    EMBO Rep. 4:699-703(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 268-488 IN COMPLEX WITH MTR2.

Entry informationi

Entry nameiMEX67_YEAST
AccessioniPrimary (citable) accession number: Q99257
Secondary accession number(s): D6W3J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2830 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.