Reviewed, UniProtKB/Swiss-Prot Q99222 (AFI1_YEAST)

Names and origin

Protein names

Recommended name:
    ARF3-interacting protein 1

Gene names

Name:	AFI1
Ordered Locus Names:	YOR129C
ORF Names:	YOR3296C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	893 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in actin patch polarization. Required for maintaining a proper budding pattern in yeast cells. Required for proper polarized localization of the ADP-ribosylation factor ARF3 at the plasma membrane. Ref.7
Subunit structure	Interacts with ARF3 (in GTP-bound form). Interacts with CNM67. Ref.7 Ref.5
Subcellular location	Cytoplasm › perinuclear region. Cytoplasm › cell cortex. Note: Enriched at the nuclear envelope and at the plasma membrane, especially in daughter cells and at the bud neck. Ref.7 Ref.5
Induction	Mainly expressed in dividing cells, but not in stationary phase cells (at protein level). Ref.7 Ref.5
Miscellaneous	Present with 768 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the AFI1/mesA family.
Sequence caution	The sequence CAA60863.1 differs from that shown. Reason: Frameshift at positions 101 and 102.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	actin cortical patch localization Ref.7 Inferred from genetic interaction. Source: SGD asymmetric protein localization Ref.7 Inferred from mutant phenotype. Source: SGD cellular bud site selection Ref.7 Inferred from mutant phenotype. Source: SGD
Cellular component	cell cortex Inferred from electronic annotation. Source: UniProtKB-SubCell cellular bud neck Ref.7 Inferred from direct assay. Source: SGD nucleus Ref.5 Ref.7 Inferred from mutant phenotype. Source: SGD perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Ref.5 Ref.7 Inferred from direct assay. Source: SGD
Molecular function	protein binding Ref.5 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CNM67	P53865	2	EBI-1781795,EBI-29172

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 893	893	ARF3-interacting protein 1		PRO_0000066256
Regions
	Region	25 – 488	464	Interaction with active ARF3
	Coiled coil	152 – 172	21	Potential
	Coiled coil	872 – 892	21	Potential
Amino acid modifications
	Modified residue	9	1	Phosphoserine Ref.8
Experimental info
	Mutagenesis	38 – 41	4	KLGP → AAAA: Abolishes interaction with ARF3. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q99222-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: EFAEBE618FBDD989	FASTA	893	102,250
	10 20 30 40 50 60 MLRRELNNSI SNRSIENESF PFERPNVSYI ISAEFDNKLG PILKHQYPKD IPGFNQFSHE 70 80 90 100 110 120 QRNGNTSVSM NLASLMIPSS IERNPGKQDI TVFTLYYNKF TQNYQLFPVP KDPRFSFNLH 130 140 150 160 170 180 HREQSDGSVT NSIYYDAENH QDAKNNRYTI VLEDDELECQ EVQNNQKAID NEPLFFINVA 190 200 210 220 230 240 NTVLDTTNDR GAVIKSIAIG TPLKTFFAFK NIIVLVLDLY MKAPTQAAAT DILLDCFNML 250 260 270 280 290 300 NSIDLTLIND IHSKSSIQEV LHSIHDESII TKVFLDPDST LKKLFCINGF DTKDKYGNIV 310 320 330 340 350 360 TFHDQLIQYH FTRFQPKTLP PFLLKIPLQF NMIRREPIYI ENDYNELVLK FLDKFVPYLL 370 380 390 400 410 420 KAGQKVNAWK LVINSTKLSK EDLCAFILSL ANITATYASD PQSYFKGNAA LIFPYMDISL 430 440 450 460 470 480 VDGLRAYVAS NSDFVGCFAI IGTANPIFRY QLDIWDYYYD VDEGVFYENN SPEKEKPDTV 490 500 510 520 530 540 AEVKIGPNPL RKIFNRPHFS TNAVNESQVN LGQKLFSLLI DEYHDSDTIM SVLRRLNVLQ 550 560 570 580 590 600 LENLLDALKR REIPPNIALK DEYIMFYKDF FIFPEFFDYF TLHSIELLSN LDNCLFSLGN 610 620 630 640 650 660 TCQLFSTEQI YSQLSQILDI VKELFRMVSV SRTNIEKFLN ACLNYSPFKI LPTAQLHGDN 670 680 690 700 710 720 ISRWSFESEV RQGFDNFNSY MGIEKDPHGV IVSAIDLFTQ IYSFDILAFF LTFITKESGQ 730 740 750 760 770 780 DLPFTKSLSR RRTYLTRIAQ SSSLRQFLQL STRPNIRILG GNGQGTGNSN YPEFTNASSV 790 800 810 820 830 840 ISPKLRASPL LERRASKICY AITKLLYRLE CHPIGMALLK KYLHNQLREA YLESKRHFIS 850 860 870 880 890 KKGDSTNTSS TIASSSFAGA SVPLSSNESG MLNGLKQINE QQESTLETTQ KED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and analysis of 51 kb on the right arm of chromosome XV from Saccharomyces cerevisiae reveals 30 open reading frames." Wiemann S., Rechmann S., Benes V., Voss H., Schwager C., Vlcek C., Stegemann J., Zimmermann J., Erfle H., Paces V., Ansorge W. Yeast 12:281-288(1996) [PubMed: 8904341] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"DNA sequencing and analysis of 130 kb from yeast chromosome XV." Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C., Schwager C., Paces V., Sander C., Ansorge W. Yeast 13:655-672(1997) [PubMed: 9200815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"The ARG11 gene of Saccharomyces cerevisiae encodes a mitochondrial integral membrane protein required for arginine biosynthesis." Crabeel M., Soetens O., de Rijcke M., Pratiwi R., Pankiewicz R. J. Biol. Chem. 271:25011-25018(1996) [PubMed: 8798783] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-157. Strain: ATCC 28383 / FL100 / VTT C-80102.
[5]	"YOR129c, a new element interacting with Cnm67p, a component of the spindle pole body of Saccharomyces cerevisiae." Wysocka M., Bialkowska A., Micialkiewicz A., Kurlandzka A. Acta Biochim. Pol. 50:883-890(2003) [PubMed: 14515169] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CNM67, INDUCTION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Afi1p functions as an Arf3p polarization-specific docking factor for development of polarity." Tsai P.-C., Lee S.-W., Liu Y.-W., Chu C.-W., Chen K.-Y., Ho J.-C., Lee F.-J. J. Biol. Chem. 283:16915-16927(2008) [PubMed: 18397879] [Abstract] Cited for: FUNCTION, INTERACTION WITH ARF3, INDUCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 38-LYS--PRO-41.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X90518 Genomic DNA. Translation: CAA62121.1. X94335 Genomic DNA. Translation: CAA64048.1. Z75037 Genomic DNA. Translation: CAA99328.1. X87414 Genomic DNA. Translation: CAA60863.1. Frameshift.
PIR	S61000.
RefSeq	NP_014772.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99222. 1 interaction.
STRING	Q99222.
Proteomic databases
PRIDE	Q99222.
Genome annotation databases
Ensembl	YOR129C; YOR129C; YOR129C; Saccharomyces cerevisiae. [Genome view]
GeneID	854296.
KEGG	sce:YOR129C.
NMPDR	fig|4932.3.peg.5875.
Organism-specific databases
CYGD	YOR129c.
SGD	S000005655. YOR129C.
Phylogenomic databases
OMA	RVNELPK
OrthoDB	EOG9BZPK4
Gene expression databases
ArrayExpress	Q99222.
Genevestigator	Q99222.
GermOnline	YOR129C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013925. SPB_interacting. [Graphical view]
Pfam	PF08616. SPB_interacting. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	976293.

Entry information

Entry name

AFI1_YEAST

Accession

Primary (citable) accession number: Q99222
Secondary accession number(s): Q92274

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2003
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)