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Protein

Protein OS-9 homolog

Gene

YOS9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lectin involved in the quality control of the secretory pathway. As a member of the endoplasmic reticulum-associated degradation lumenal (ERAD-L) surveillance system, targets misfolded endoplasmic reticulum lumenal glycoproteins for degradation. The recognition of targets is N-glycan specific.6 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei137CarbohydrateBy similarity1
Binding sitei194CarbohydrateBy similarity1
Binding sitei200CarbohydrateBy similarity1
Binding sitei223CarbohydrateBy similarity1
Binding sitei229CarbohydrateBy similarity1

GO - Molecular functioni

  • oligosaccharide binding Source: SGD

GO - Biological processi

  • endoplasmic reticulum unfolded protein response Source: SGD
  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • glycoprotein ERAD pathway Source: SGD
  • retrograde protein transport, ER to cytosol Source: SGD
Complete GO annotation...

Keywords - Ligandi

Lectin

Enzyme and pathway databases

BioCyciYEAST:G3O-29666-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein OS-9 homolog
Gene namesi
Name:YOS9
Ordered Locus Names:YDR057W
ORF Names:D4222
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR057W.
SGDiS000002464. YOS9.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum lumen Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • luminal surveillance complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Interaction of substrate with HRD1 is reduced; in YOS9 and USA1 double mutants this interaction is completely abolished. Interaction of substrate (either glycosylated or non-glycosylated) with HRD3 is not affected.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi127Y → A: Decrease of ER lumenal misfolded protein degradation. 1 Publication1
Mutagenesisi137Q → E: Decrease of ER lumenal misfolded protein degradation. 1 Publication1
Mutagenesisi139H → A: Decrease of ER lumenal misfolded protein degradation. 1 Publication1
Mutagenesisi200R → A: Decrease of ER lumenal misfolded protein degradation. No effect on interaction with CDC48, HRD3, KAR2, UBX2, HRD1 or EMP47. 3 Publications1
Mutagenesisi223E → N: Decrease of ER lumenal misfolded protein degradation. 2 Publications1
Mutagenesisi229Y → A or F: Decrease of ER lumenal misfolded protein degradation. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000004275822 – 542Protein OS-9 homologAdd BLAST521

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi52N-linked (GlcNAc...)Sequence analysis1
Glycosylationi74N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi117 ↔ 130By similarity
Disulfide bondi193 ↔ 227By similarity
Disulfide bondi208 ↔ 239By similarity
Glycosylationi380N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ99220.
PRIDEiQ99220.

PTM databases

iPTMnetiQ99220.

Interactioni

Subunit structurei

Component of the HRD1 complex which contains HRD1, HRD3, USA1, DER1, YOS9, CDC48, NPL4, UFD1 AND UBX2/SEL1. The complex is composed of the core membrane complex, consisting of the E3 ligase HRD1 and its cofactors HRD3, DER1 and USA1, the substrate recruiting factor YOS9, and the heterotrimeric UFD1-NPL4-CDC48/p97 (UNC) ATPase complex recruited by UBX2/SEL1. Interacts with DER1, USA1, UBX2, CDC48, HRD1, KAR2 and HRD3. Interacts with EMP47. Interacts with misfolded ER lumenal proteins like PCR1. Interacts with the GPI-anchored proteins GAS1 and MKC7.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HRD1Q081096EBI-34938,EBI-37613
HRD3Q057878EBI-34938,EBI-31647
KAR2P164742EBI-34938,EBI-7876
PRC1P007292EBI-34938,EBI-4153
UBX2Q042283EBI-34938,EBI-27730

Protein-protein interaction databases

BioGridi32110. 43 interactors.
IntActiQ99220. 11 interactors.
MINTiMINT-6490923.

Structurei

Secondary structure

1542
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi276 – 283Combined sources8
Beta strandi286 – 293Combined sources8
Beta strandi299 – 302Combined sources4
Helixi305 – 307Combined sources3
Beta strandi310 – 312Combined sources3
Helixi315 – 330Combined sources16
Beta strandi347 – 355Combined sources9
Beta strandi361 – 369Combined sources9
Beta strandi371 – 373Combined sources3
Beta strandi375 – 380Combined sources6
Beta strandi391 – 396Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YMAX-ray2.54A/B266-424[»]
ProteinModelPortaliQ99220.
SMRiQ99220.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini115 – 190PRKCSHAdd BLAST76

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi539 – 542Prevents secretion from ERPROSITE-ProRule annotation4

Domaini

The MHR domain (mannose 6-phosphate receptor homology) is required for the ERAD-L activity.2 Publications

Sequence similaritiesi

Belongs to the OS-9 family.Curated
Contains 1 PRKCSH domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiQ99220.
KOiK10088.
OMAiVEIQYVC.
OrthoDBiEOG092C4FPG.

Family and domain databases

InterProiIPR012913. PRKCSH.
[Graphical view]
PfamiPF07915. PRKCSH. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAKIIYALS AISALIPLGS SLLAPIEDPI VSNKYLISYI DEDDWSDRIL
60 70 80 90 100
QNQSVMNSGY IVNMGDDLEC FIQNASTQLN DVLEDSNEHS NSEKTALLTK
110 120 130 140 150
TLNQGVKTIF DKLNERCIFY QAGFWIYEYC PGIEFVQFHG RVNTKTGEIV
160 170 180 190 200
NRDESLVYRL GKPKANVEER EFELLYDDVG YYISEIIGSG DICDVTGAER
210 220 230 240 250
MVEIQYVCGG SNSGPSTIQW VRETKICVYE AQVTIPELCN LELLAKNEDQ
260 270 280 290 300
KNASPILCRM PAKSKIGSNS IDLITKYEPI FLGSGIYFLR PFNTDERDKL
310 320 330 340 350
MVTDNAMSNW DEITETYYQK FGNAINKMLS LRLVSLPNGH ILQPGDSCVW
360 370 380 390 400
LAEVVDMKDR FQTTLSLNIL NSQRAEIFFN KTFTFNEDNG NFLSYKIGDH
410 420 430 440 450
GESTELGQIT HSNKADINTA EIRSDEYLIN TDNELFLRIS KEIAEVKELL
460 470 480 490 500
NEIVSPHEME VIFENMRNQP NNDFELALMN KLKSSLNDDN KVEQINNARM
510 520 530 540
DDDESTSHTT RDIGEAGSQT TGNTESEVTN VAAGVFIEHD EL
Length:542
Mass (Da):61,258
Last modified:November 1, 1996 - v1
Checksum:iBF353A0278703BE8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25P → L in CAA89086 (PubMed:9169867).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84162 Genomic DNA. Translation: CAA58973.1.
Z74353 Genomic DNA. Translation: CAA98875.1.
Z49209 Genomic DNA. Translation: CAA89086.1.
BK006938 Genomic DNA. Translation: DAA11903.1.
PIRiS58837.
RefSeqiNP_010342.3. NM_001180365.3.

Genome annotation databases

EnsemblFungiiYDR057W; YDR057W; YDR057W.
GeneIDi851627.
KEGGisce:YDR057W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84162 Genomic DNA. Translation: CAA58973.1.
Z74353 Genomic DNA. Translation: CAA98875.1.
Z49209 Genomic DNA. Translation: CAA89086.1.
BK006938 Genomic DNA. Translation: DAA11903.1.
PIRiS58837.
RefSeqiNP_010342.3. NM_001180365.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YMAX-ray2.54A/B266-424[»]
ProteinModelPortaliQ99220.
SMRiQ99220.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32110. 43 interactors.
IntActiQ99220. 11 interactors.
MINTiMINT-6490923.

PTM databases

iPTMnetiQ99220.

Proteomic databases

MaxQBiQ99220.
PRIDEiQ99220.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR057W; YDR057W; YDR057W.
GeneIDi851627.
KEGGisce:YDR057W.

Organism-specific databases

EuPathDBiFungiDB:YDR057W.
SGDiS000002464. YOS9.

Phylogenomic databases

InParanoidiQ99220.
KOiK10088.
OMAiVEIQYVC.
OrthoDBiEOG092C4FPG.

Enzyme and pathway databases

BioCyciYEAST:G3O-29666-MONOMER.

Miscellaneous databases

PROiQ99220.

Family and domain databases

InterProiIPR012913. PRKCSH.
[Graphical view]
PfamiPF07915. PRKCSH. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOS9_YEAST
AccessioniPrimary (citable) accession number: Q99220
Secondary accession number(s): D6VS43, Q04313
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.