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Q99219

- YI31A_YEAST

UniProt

Q99219 - YI31A_YEAST

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Protein
Transposon Ty3-I Gag polyprotein
Gene
TY3A-I, YILWTy3-1 GAG, YIL082W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Capsid protein (CA) is the structural component of the virus-like particle (VLP), forming the shell that encapsulates the retrotransposons dimeric RNA genome.
Nucleocapsid protein p9 (NC) forms the nucleocore that coats the retro-elements dimeric RNA. Binds these RNAs through its zinc fingers By similarity. Promotes primer tRNA(i)-Met annealing to the multipartite primer-binding site (PBS), dimerization of Ty3 RNA and initiation of reverse transcription.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei207 – 2082Cleavage; by Ty3 protease
Sitei233 – 2342Cleavage; by Ty3 protease

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri265 – 28218CCHC-type
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: SGD
  2. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. transposition, RNA-mediated Source: SGD
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transposon Ty3-I Gag polyprotein
Alternative name(s):
Gag3
Transposon Ty3-2 protein A
Short name:
TY3A
Cleaved into the following 3 chains:
Capsid protein
Short name:
CA
Alternative name(s):
p24
Alternative name(s):
p7
Gene namesi
Name:TY3A-I
Synonyms:YILWTy3-1 GAG
Ordered Locus Names:YIL082W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIL082w.
SGDiS000001344. YIL082W.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. retrotransposon nucleocapsid Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 290289Transposon Ty3-I Gag polyprotein
PRO_0000279376Add
BLAST
Chaini2 – 207206Capsid protein
PRO_0000279377Add
BLAST
Peptidei208 – 23326Spacer peptide p3
PRO_0000279378Add
BLAST
Chaini234 – 29057Nucleocapsid protein p9
PRO_0000279379Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ99219.

Expressioni

Gene expression databases

GenevestigatoriQ99219.

Interactioni

Protein-protein interaction databases

DIPiDIP-8996N.
IntActiQ99219. 1 interaction.
MINTiMINT-394390.
STRINGi4932.YIL082W.

Structurei

3D structure databases

ProteinModelPortaliQ99219.

Family & Domainsi

Domaini

The N-terminal domain of NC, but not its zinc finger, is required for nucleoprotein complex formation and its chaperone activities.

Sequence similaritiesi

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG242522.
GeneTreeiENSGT00740000115124.
HOGENOMiHOG000155559.
OrthoDBiEOG7HF1TD.

Family and domain databases

InterProiIPR005162. Retrotrans_gag_dom.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF03732. Retrotrans_gag. 1 hit.
[Graphical view]
SMARTiSM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF57756. SSF57756. 1 hit.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Note: The Gag-Pol polyprotein is generated by a +1 ribosomal frameshift.

Isoform Transposon Ty3-I Gag polyprotein (identifier: Q99219-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSFMDQIPGG GNYPKLPVEC LPNFPIQPSL TFRGRNDSHK LKNFISEIML    50
NMSMISWPND ASRIVYCRRH LLNPAAQWAN DFVQEQGILE ITFDTFIQGL 100
YQHFYKPPDI NKIFNAITQL SEAKLGIERL NQRFRKIWDR MPPDFMTEKA 150
AIMTYTRLLT KETYNIVRMH KPETLKDAME EAYQTTALTE RFFPGFELDA 200
DGDTIIGATT HLQEEYDSDY DSEDNLTQNR YVHTVRTRRS YNKPMSNHRN 250
RRNNNASREE CIKNRLCFYC KKEGHRLNEC RARKASSNRS 290

Note: Produced by conventional translation.

Length:290
Mass (Da):34,100
Last modified:November 1, 1996 - v1
Checksum:i5113F9717ADD196A
GO
Isoform Transposon Ty3-I Gag-Pol polyprotein (identifier: Q7LHG5-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q7LHG5.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by +1 ribosomal frameshifting between codon Ala-285 and Val-286 of the YIL082W ORF.

Length:1,498
Mass (Da):172,369
GO

Sequence cautioni

The sequence AAA35183.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M23367 Genomic DNA. Translation: AAA35183.1. Different initiation.
Z46728 Genomic DNA. Translation: CAA86712.1.
AY557875 Genomic DNA. Translation: AAS56201.1.
BK006942 Genomic DNA. No translation available.
PIRiS41555.

Genome annotation databases

EnsemblFungiiYIL082W; YIL082W; YIL082W. [Q99219-1]

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M23367 Genomic DNA. Translation: AAA35183.1 . Different initiation.
Z46728 Genomic DNA. Translation: CAA86712.1 .
AY557875 Genomic DNA. Translation: AAS56201.1 .
BK006942 Genomic DNA. No translation available.
PIRi S41555.

3D structure databases

ProteinModelPortali Q99219.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-8996N.
IntActi Q99219. 1 interaction.
MINTi MINT-394390.
STRINGi 4932.YIL082W.

Proteomic databases

PaxDbi Q99219.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIL082W ; YIL082W ; YIL082W . [Q99219-1 ]

Organism-specific databases

CYGDi YIL082w.
SGDi S000001344. YIL082W.

Phylogenomic databases

eggNOGi NOG242522.
GeneTreei ENSGT00740000115124.
HOGENOMi HOG000155559.
OrthoDBi EOG7HF1TD.

Gene expression databases

Genevestigatori Q99219.

Family and domain databases

InterProi IPR005162. Retrotrans_gag_dom.
IPR001878. Znf_CCHC.
[Graphical view ]
Pfami PF03732. Retrotrans_gag. 1 hit.
[Graphical view ]
SMARTi SM00343. ZnF_C2HC. 1 hit.
[Graphical view ]
SUPFAMi SSF57756. SSF57756. 1 hit.
PROSITEi PS50158. ZF_CCHC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Ty3, a yeast retrotransposon associated with tRNA genes, has homology to animal retroviruses."
    Hansen L.J., Chalker D.L., Sandmeyer S.B.
    Mol. Cell. Biol. 8:5245-5256(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Transposable elements and genome organization: a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence."
    Kim J.M., Vanguri S., Boeke J.D., Gabriel A., Voytas D.F.
    Genome Res. 8:464-478(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.
  6. "Happy together: the life and times of Ty retrotransposons and their hosts."
    Lesage P., Todeschini A.L.
    Cytogenet. Genome Res. 110:70-90(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiYI31A_YEAST
AccessioniPrimary (citable) accession number: Q99219
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Retrotransposons are mobile genetic entities that are able to replicate via an RNA intermediate and a reverse transcription step. In contrast to retroviruses, retrotransposons are non-infectious, lack an envelope and remain intracellular. Ty3 retrotransposons belong to the gypsy-like elements (metaviridae).

Keywords - Technical termi

Complete proteome, Reference proteome, Transposable element

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

Similar proteinsi