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Q99219

- YI31A_YEAST

UniProt

Q99219 - YI31A_YEAST

Protein

Transposon Ty3-I Gag polyprotein

Gene

TY3A-I

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Capsid protein (CA) is the structural component of the virus-like particle (VLP), forming the shell that encapsulates the retrotransposons dimeric RNA genome.
    Nucleocapsid protein p9 (NC) forms the nucleocore that coats the retro-elements dimeric RNA. Binds these RNAs through its zinc fingers By similarity. Promotes primer tRNA(i)-Met annealing to the multipartite primer-binding site (PBS), dimerization of Ty3 RNA and initiation of reverse transcription.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei207 – 2082Cleavage; by Ty3 protease
    Sitei233 – 2342Cleavage; by Ty3 protease

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri265 – 28218CCHC-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: SGD
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. transposition, RNA-mediated Source: SGD

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transposon Ty3-I Gag polyprotein
    Alternative name(s):
    Gag3
    Transposon Ty3-2 protein A
    Short name:
    TY3A
    Cleaved into the following 3 chains:
    Capsid protein
    Short name:
    CA
    Alternative name(s):
    p24
    Alternative name(s):
    p7
    Gene namesi
    Name:TY3A-I
    Synonyms:YILWTy3-1 GAG
    Ordered Locus Names:YIL082W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL082w.
    SGDiS000001344. YIL082W.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. retrotransposon nucleocapsid Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 290289Transposon Ty3-I Gag polyproteinPRO_0000279376Add
    BLAST
    Chaini2 – 207206Capsid proteinPRO_0000279377Add
    BLAST
    Peptidei208 – 23326Spacer peptide p3PRO_0000279378Add
    BLAST
    Chaini234 – 29057Nucleocapsid protein p9PRO_0000279379Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ99219.

    Expressioni

    Gene expression databases

    GenevestigatoriQ99219.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-8996N.
    IntActiQ99219. 1 interaction.
    MINTiMINT-394390.
    STRINGi4932.YIL082W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99219.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The N-terminal domain of NC, but not its zinc finger, is required for nucleoprotein complex formation and its chaperone activities.

    Sequence similaritiesi

    Contains 1 CCHC-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri265 – 28218CCHC-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG242522.
    GeneTreeiENSGT00740000115124.
    HOGENOMiHOG000155559.
    OrthoDBiEOG7HF1TD.

    Family and domain databases

    InterProiIPR005162. Retrotrans_gag_dom.
    IPR001878. Znf_CCHC.
    [Graphical view]
    PfamiPF03732. Retrotrans_gag. 1 hit.
    [Graphical view]
    SMARTiSM00343. ZnF_C2HC. 1 hit.
    [Graphical view]
    SUPFAMiSSF57756. SSF57756. 1 hit.
    PROSITEiPS50158. ZF_CCHC. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

    Note: The Gag-Pol polyprotein is generated by a +1 ribosomal frameshift.

    Isoform Transposon Ty3-I Gag polyprotein (identifier: Q99219-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSFMDQIPGG GNYPKLPVEC LPNFPIQPSL TFRGRNDSHK LKNFISEIML    50
    NMSMISWPND ASRIVYCRRH LLNPAAQWAN DFVQEQGILE ITFDTFIQGL 100
    YQHFYKPPDI NKIFNAITQL SEAKLGIERL NQRFRKIWDR MPPDFMTEKA 150
    AIMTYTRLLT KETYNIVRMH KPETLKDAME EAYQTTALTE RFFPGFELDA 200
    DGDTIIGATT HLQEEYDSDY DSEDNLTQNR YVHTVRTRRS YNKPMSNHRN 250
    RRNNNASREE CIKNRLCFYC KKEGHRLNEC RARKASSNRS 290

    Note: Produced by conventional translation.

    Length:290
    Mass (Da):34,100
    Last modified:November 1, 1996 - v1
    Checksum:i5113F9717ADD196A
    GO
    Isoform Transposon Ty3-I Gag-Pol polyprotein (identifier: Q7LHG5-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry Q7LHG5.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: Produced by +1 ribosomal frameshifting between codon Ala-285 and Val-286 of the YIL082W ORF.

    Length:1,498
    Mass (Da):172,369
    GO

    Sequence cautioni

    The sequence AAA35183.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M23367 Genomic DNA. Translation: AAA35183.1. Different initiation.
    Z46728 Genomic DNA. Translation: CAA86712.1.
    AY557875 Genomic DNA. Translation: AAS56201.1.
    BK006942 Genomic DNA. No translation available.
    PIRiS41555.

    Genome annotation databases

    EnsemblFungiiYIL082W; YIL082W; YIL082W. [Q99219-1]

    Keywords - Coding sequence diversityi

    Ribosomal frameshifting

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M23367 Genomic DNA. Translation: AAA35183.1 . Different initiation.
    Z46728 Genomic DNA. Translation: CAA86712.1 .
    AY557875 Genomic DNA. Translation: AAS56201.1 .
    BK006942 Genomic DNA. No translation available.
    PIRi S41555.

    3D structure databases

    ProteinModelPortali Q99219.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-8996N.
    IntActi Q99219. 1 interaction.
    MINTi MINT-394390.
    STRINGi 4932.YIL082W.

    Proteomic databases

    PaxDbi Q99219.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL082W ; YIL082W ; YIL082W . [Q99219-1 ]

    Organism-specific databases

    CYGDi YIL082w.
    SGDi S000001344. YIL082W.

    Phylogenomic databases

    eggNOGi NOG242522.
    GeneTreei ENSGT00740000115124.
    HOGENOMi HOG000155559.
    OrthoDBi EOG7HF1TD.

    Gene expression databases

    Genevestigatori Q99219.

    Family and domain databases

    InterProi IPR005162. Retrotrans_gag_dom.
    IPR001878. Znf_CCHC.
    [Graphical view ]
    Pfami PF03732. Retrotrans_gag. 1 hit.
    [Graphical view ]
    SMARTi SM00343. ZnF_C2HC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57756. SSF57756. 1 hit.
    PROSITEi PS50158. ZF_CCHC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Ty3, a yeast retrotransposon associated with tRNA genes, has homology to animal retroviruses."
      Hansen L.J., Chalker D.L., Sandmeyer S.B.
      Mol. Cell. Biol. 8:5245-5256(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Transposable elements and genome organization: a comprehensive survey of retrotransposons revealed by the complete Saccharomyces cerevisiae genome sequence."
      Kim J.M., Vanguri S., Boeke J.D., Gabriel A., Voytas D.F.
      Genome Res. 8:464-478(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE.
    6. "Happy together: the life and times of Ty retrotransposons and their hosts."
      Lesage P., Todeschini A.L.
      Cytogenet. Genome Res. 110:70-90(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiYI31A_YEAST
    AccessioniPrimary (citable) accession number: Q99219
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 6, 2007
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Retrotransposons are mobile genetic entities that are able to replicate via an RNA intermediate and a reverse transcription step. In contrast to retroviruses, retrotransposons are non-infectious, lack an envelope and remain intracellular. Ty3 retrotransposons belong to the gypsy-like elements (metaviridae).

    Keywords - Technical termi

    Complete proteome, Reference proteome, Transposable element

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3