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Protein

Protein SRN2

Gene

SRN2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for normal endocytic and biosynthetic traffic to the yeast vacuole.

GO - Biological processi

  • ATP export Source: SGD
  • protein targeting to membrane Source: SGD
  • protein targeting to vacuole Source: SGD
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32264-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SRN2
Alternative name(s):
ESCRT-I complex subunit VPS37
Vacuolar protein sorting-associated protein 37
Gene namesi
Name:SRN2
Synonyms:SRN10, VPS37
Ordered Locus Names:YLR119W
ORF Names:L2958, L9233.5
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR119W.
SGDiS000004109. SRN2.

Subcellular locationi

GO - Cellular componenti

  • endosome Source: SGD
  • ESCRT I complex Source: SGD
  • late endosome membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67L → D: Defective in ESCRT-I cargo sorting. 1 Publication1
Mutagenesisi75L → D: Defective in ESCRT-I cargo sorting. 1 Publication1
Mutagenesisi79V → D: Defective in ESCRT-I cargo sorting. 1 Publication1
Mutagenesisi88F → D: Defective in ESCRT-I cargo sorting; when associated with D-99. 1 Publication1
Mutagenesisi92I → D: Defective in ESCRT-I cargo sorting; when associated with D-102. 1 Publication1
Mutagenesisi95F → D: Defective in ESCRT-I cargo sorting; when associated with D-99. 1 Publication1
Mutagenesisi99F → D: Defective in ESCRT-I cargo sorting; when associated with D-88. 1 Publication1
Mutagenesisi99F → D: Defective in ESCRT-I cargo sorting; when associated with D-95. 1 Publication1
Mutagenesisi102L → D: Defective in ESCRT-I cargo sorting; when associated with D-92. 1 Publication1
Mutagenesisi181L → R: Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVB); when associated with R-185. 1 Publication1
Mutagenesisi185I → R: Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVB); when associated with R-181. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000721911 – 213Protein SRN2Add BLAST213

Proteomic databases

MaxQBiQ99176.
PRIDEiQ99176.

Interactioni

Subunit structurei

Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of STP22, VPS28, SRN2 and MVB12 in a 1:1:1:1 stoechiometry. Interacts with STP22 and MVB12.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MVB12P429396EBI-18076,EBI-23478
STP22P256045EBI-18076,EBI-411625

Protein-protein interaction databases

BioGridi31391. 178 interactors.
DIPiDIP-2130N.
IntActiQ99176. 4 interactors.
MINTiMINT-505977.

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi23 – 33Combined sources11
Helixi55 – 57Combined sources3
Helixi62 – 70Combined sources9
Helixi72 – 78Combined sources7
Helixi79 – 82Combined sources4
Helixi86 – 143Combined sources58
Beta strandi144 – 146Combined sources3
Helixi147 – 170Combined sources24
Helixi178 – 205Combined sources28

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CAZX-ray3.60C/F130-213[»]
2F66X-ray2.80C/F132-213[»]
2P22X-ray2.70C22-213[»]
ProteinModelPortaliQ99176.
SMRiQ99176.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99176.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini128 – 213VPS37 C-terminalPROSITE-ProRule annotationAdd BLAST86

Sequence similaritiesi

Belongs to the VPS37 family.Curated
Contains 1 VPS37 C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000154416.
InParanoidiQ99176.
OMAiNERTEYH.
OrthoDBiEOG092C5CB3.

Family and domain databases

InterProiIPR017435. ESCRT-1_cplx_Vps37_fungi.
IPR009851. Mod_r.
[Graphical view]
PfamiPF07200. Mod_r. 1 hit.
[Graphical view]
PIRSFiPIRSF038214. ESCRT1_Vps37_fungi. 1 hit.
PROSITEiPS51314. VPS37_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99176-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVKATKLRI KQRRKNKGLN ISRLDIIRAE MDVVPSPGLP EKVNEKSKNI
60 70 80 90 100
PLPEGINLLS SKEIIDLIQT HRHQLELYVT KFNPLTDFAG KIHAFRDQFK
110 120 130 140 150
QLEENFEDLH EQKDKVQALL ENCRILESKY VASWQDYHSE FSKKYGDIAL
160 170 180 190 200
KKKLEQNTKK LDEESSQLET TTRSIDSADD LDQFIKNYLD IRTQYHLRRE
210
KLATWDKQGN LKY
Length:213
Mass (Da):25,101
Last modified:November 1, 1997 - v1
Checksum:i4E99806CED03E345
GO

Sequence cautioni

The sequence AAB88577 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90G → E in AAB88577 (PubMed:9790597).Curated1
Sequence conflicti140E → D in AAB88577 (PubMed:9790597).Curated1
Sequence conflicti143K → E in AAB88577 (PubMed:9790597).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40562 Genomic DNA. Translation: AAB88577.1. Different initiation.
X89514 Genomic DNA. Translation: CAA61698.1.
U53877 Genomic DNA. Translation: AAB82366.1.
Z73291 Genomic DNA. Translation: CAA97687.1.
BK006945 Genomic DNA. Translation: DAA09433.1.
PIRiS64956.
RefSeqiNP_013220.1. NM_001182006.1.

Genome annotation databases

EnsemblFungiiYLR119W; YLR119W; YLR119W.
GeneIDi850810.
KEGGisce:YLR119W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40562 Genomic DNA. Translation: AAB88577.1. Different initiation.
X89514 Genomic DNA. Translation: CAA61698.1.
U53877 Genomic DNA. Translation: AAB82366.1.
Z73291 Genomic DNA. Translation: CAA97687.1.
BK006945 Genomic DNA. Translation: DAA09433.1.
PIRiS64956.
RefSeqiNP_013220.1. NM_001182006.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CAZX-ray3.60C/F130-213[»]
2F66X-ray2.80C/F132-213[»]
2P22X-ray2.70C22-213[»]
ProteinModelPortaliQ99176.
SMRiQ99176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31391. 178 interactors.
DIPiDIP-2130N.
IntActiQ99176. 4 interactors.
MINTiMINT-505977.

Proteomic databases

MaxQBiQ99176.
PRIDEiQ99176.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR119W; YLR119W; YLR119W.
GeneIDi850810.
KEGGisce:YLR119W.

Organism-specific databases

EuPathDBiFungiDB:YLR119W.
SGDiS000004109. SRN2.

Phylogenomic databases

HOGENOMiHOG000154416.
InParanoidiQ99176.
OMAiNERTEYH.
OrthoDBiEOG092C5CB3.

Enzyme and pathway databases

BioCyciYEAST:G3O-32264-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ99176.
PROiQ99176.

Family and domain databases

InterProiIPR017435. ESCRT-1_cplx_Vps37_fungi.
IPR009851. Mod_r.
[Graphical view]
PfamiPF07200. Mod_r. 1 hit.
[Graphical view]
PIRSFiPIRSF038214. ESCRT1_Vps37_fungi. 1 hit.
PROSITEiPS51314. VPS37_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRN2_YEAST
AccessioniPrimary (citable) accession number: Q99176
Secondary accession number(s): D6VYB7, Q02806
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.