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Protein

Protein SRN2

Gene

SRN2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for normal endocytic and biosynthetic traffic to the yeast vacuole.

GO - Biological processi

  • ATP export Source: SGD
  • endosome transport via multivesicular body sorting pathway Source: InterPro
  • protein targeting to membrane Source: SGD
  • protein targeting to vacuole Source: SGD
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32264-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SRN2
Alternative name(s):
ESCRT-I complex subunit VPS37
Vacuolar protein sorting-associated protein 37
Gene namesi
Name:SRN2
Synonyms:SRN10, VPS37
Ordered Locus Names:YLR119W
ORF Names:L2958, L9233.5
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR119W
SGDiS000004109 SRN2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67L → D: Defective in ESCRT-I cargo sorting. 1 Publication1
Mutagenesisi75L → D: Defective in ESCRT-I cargo sorting. 1 Publication1
Mutagenesisi79V → D: Defective in ESCRT-I cargo sorting. 1 Publication1
Mutagenesisi88F → D: Defective in ESCRT-I cargo sorting; when associated with D-99. 1 Publication1
Mutagenesisi92I → D: Defective in ESCRT-I cargo sorting; when associated with D-102. 1 Publication1
Mutagenesisi95F → D: Defective in ESCRT-I cargo sorting; when associated with D-99. 1 Publication1
Mutagenesisi99F → D: Defective in ESCRT-I cargo sorting; when associated with D-88. 1 Publication1
Mutagenesisi99F → D: Defective in ESCRT-I cargo sorting; when associated with D-95. 1 Publication1
Mutagenesisi102L → D: Defective in ESCRT-I cargo sorting; when associated with D-92. 1 Publication1
Mutagenesisi181L → R: Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVB); when associated with R-185. 1 Publication1
Mutagenesisi185I → R: Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVB); when associated with R-181. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000721911 – 213Protein SRN2Add BLAST213

Proteomic databases

MaxQBiQ99176
PaxDbiQ99176
PRIDEiQ99176

Interactioni

Subunit structurei

Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of STP22, VPS28, SRN2 and MVB12 in a 1:1:1:1 stoichiometry. Interacts with STP22 and MVB12.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31391, 465 interactors
DIPiDIP-2130N
IntActiQ99176, 9 interactors
MINTiQ99176
STRINGi4932.YLR119W

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi23 – 33Combined sources11
Helixi55 – 57Combined sources3
Helixi62 – 70Combined sources9
Helixi72 – 78Combined sources7
Helixi79 – 82Combined sources4
Helixi86 – 143Combined sources58
Beta strandi144 – 146Combined sources3
Helixi147 – 170Combined sources24
Helixi178 – 205Combined sources28

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CAZX-ray3.60C/F130-213[»]
2F66X-ray2.80C/F132-213[»]
2P22X-ray2.70C22-213[»]
ProteinModelPortaliQ99176
SMRiQ99176
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ99176

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini128 – 213VPS37 C-terminalPROSITE-ProRule annotationAdd BLAST86

Sequence similaritiesi

Belongs to the VPS37 family.Curated

Phylogenomic databases

HOGENOMiHOG000154416
InParanoidiQ99176
OMAiNERTEYH
OrthoDBiEOG092C5CB3

Family and domain databases

Gene3Di1.10.287.660, 1 hit
InterProiView protein in InterPro
IPR017435 ESCRT-1_cplx_Vps37_fungi
IPR037202 ESCRT_assembly_dom
IPR029012 Helix_hairpin_bin_sf
IPR009851 Mod_r
IPR037859 Vps37
PANTHERiPTHR13678 PTHR13678, 1 hit
PfamiView protein in Pfam
PF07200 Mod_r, 1 hit
PIRSFiPIRSF038214 ESCRT1_Vps37_fungi, 1 hit
SUPFAMiSSF140111 SSF140111, 1 hit
PROSITEiView protein in PROSITE
PS51314 VPS37_C, 1 hit

Sequencei

Sequence statusi: Complete.

Q99176-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVKATKLRI KQRRKNKGLN ISRLDIIRAE MDVVPSPGLP EKVNEKSKNI
60 70 80 90 100
PLPEGINLLS SKEIIDLIQT HRHQLELYVT KFNPLTDFAG KIHAFRDQFK
110 120 130 140 150
QLEENFEDLH EQKDKVQALL ENCRILESKY VASWQDYHSE FSKKYGDIAL
160 170 180 190 200
KKKLEQNTKK LDEESSQLET TTRSIDSADD LDQFIKNYLD IRTQYHLRRE
210
KLATWDKQGN LKY
Length:213
Mass (Da):25,101
Last modified:November 1, 1997 - v1
Checksum:i4E99806CED03E345
GO

Sequence cautioni

The sequence AAB88577 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti90G → E in AAB88577 (PubMed:9790597).Curated1
Sequence conflicti140E → D in AAB88577 (PubMed:9790597).Curated1
Sequence conflicti143K → E in AAB88577 (PubMed:9790597).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40562 Genomic DNA Translation: AAB88577.1 Different initiation.
X89514 Genomic DNA Translation: CAA61698.1
U53877 Genomic DNA Translation: AAB82366.1
Z73291 Genomic DNA Translation: CAA97687.1
BK006945 Genomic DNA Translation: DAA09433.1
PIRiS64956
RefSeqiNP_013220.1, NM_001182006.1

Genome annotation databases

EnsemblFungiiYLR119W; YLR119W; YLR119W
GeneIDi850810
KEGGisce:YLR119W

Similar proteinsi

Entry informationi

Entry nameiSRN2_YEAST
AccessioniPrimary (citable) accession number: Q99176
Secondary accession number(s): D6VYB7, Q02806
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health