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Protein

Alpha-galactosidase

Gene

MEL

Organism
Lachancea cidri (Yeast) (Zygosaccharomyces cidri)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711SubstrateBy similarity
Binding sitei72 – 721SubstrateBy similarity
Binding sitei146 – 1461SubstrateBy similarity
Active sitei148 – 1481NucleophileBy similarity
Binding sitei204 – 2041SubstrateBy similarity
Active sitei208 – 2081Proton donorBy similarity
Binding sitei250 – 2501SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase (EC:3.2.1.22)
Alternative name(s):
Alpha-D-galactoside galactohydrolase
Melibiase
Gene namesi
Name:MEL
OrganismiLachancea cidri (Yeast) (Zygosaccharomyces cidri)
Taxonomic identifieri29831 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeLachancea

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence analysisAdd
BLAST
Chaini18 – 469452Alpha-galactosidasePRO_0000001011Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 73By similarity
Glycosylationi81 – 811N-linked (GlcNAc...)Sequence analysis
Disulfide bondi120 ↔ 150By similarity
Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence analysis
Disulfide bondi220 ↔ 236By similarity
Disulfide bondi222 ↔ 229By similarity
Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence analysis
Glycosylationi369 – 3691N-linked (GlcNAc...)Sequence analysis
Glycosylationi402 – 4021N-linked (GlcNAc...)Sequence analysis
Glycosylationi412 – 4121N-linked (GlcNAc...)Sequence analysis
Glycosylationi421 – 4211N-linked (GlcNAc...)Sequence analysis
Glycosylationi426 – 4261N-linked (GlcNAc...)Sequence analysis
Glycosylationi434 – 4341N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ99172.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_27/36_CS.
IPR013780. Glyco_hydro_b.
IPR006215. Glyco_hydro_melibiase.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF16499. Melibiase_2. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
PR00748. MELIBIASE.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99172-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFPFFFALFF SSTDVLAASP SYNGLGLTPQ MGWDNWNSFG CSVKEELLLG
60 70 80 90 100
TAEKIVKLGL KDLGYNYIIL DDCWSSGRSS NGSLLADDSK FPHGMKYVAE
110 120 130 140 150
QLHNSQLKFG MYSSAGEYTC AGYAGSLGYE DMDAATFASW DVDYLKYDNC
160 170 180 190 200
YNKGEFGTPE ISYKRYKAMS DALNKTGRPI FYSLCNWGQD LTFYWGSAIS
210 220 230 240 250
NSWRMSGDVY PQFDRPDSRC PCSGDEYDCS YPGFHCSIMN ILNKAAPMGQ
260 270 280 290 300
NAAPGGWNDL DMLEVGVGNM SDSEEVAHFS MWAIVKSPLI IGADIDDLKD
310 320 330 340 350
SSLSVYSNPA VIAINQDVLG TPATRIWKYH VSDKDQYGEG EIQLWSGPLD
360 370 380 390 400
NGDHVVALLN GGNNERSMNA SWNDIFIDYL ADSDELSNTW GLYDLWARRM
410 420 430 440 450
SNATAASILS GNMTSAGLNY NVTQLNYTAG IARNDSRLFG DRVVELSKGE
460
SLTATVPGHG VALFRLRPE
Length:469
Mass (Da):51,737
Last modified:November 1, 1996 - v1
Checksum:i1720331848F46594
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24957 Genomic DNA. Translation: AAA35280.1.
PIRiS45453.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24957 Genomic DNA. Translation: AAA35280.1.
PIRiS45453.

3D structure databases

ProteinModelPortaliQ99172.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_27/36_CS.
IPR013780. Glyco_hydro_b.
IPR006215. Glyco_hydro_melibiase.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF16499. Melibiase_2. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
PR00748. MELIBIASE.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of MEL genes in the genus Zygosaccharomyces."
    Turakainen H., Hankaanpaa M., Korhola M., Aho S.
    Yeast 10:733-745(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMEL_LACCI
AccessioniPrimary (citable) accession number: Q99172
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.