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Reviewed, UniProtKB/Swiss-Prot Q99172 (MEL_ZYGCI)

Last modified June 16, 2009. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alpha-galactosidase
    EC=3.2.1.22
Alternative name(s):
    Alpha-D-galactoside galactohydrolase
    Melibiase
Gene names
Name: MEL
OrganismZygosaccharomyces cidri
Taxonomic identifier29831 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Lachancea

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Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 27 family.

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Ontologies

Keywords
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionalpha-galactosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 469452Alpha-galactosidase
PRO_0000001011

Sites

Active site1481Nucleophile By similarity
Active site2081Proton donor By similarity

Amino acid modifications

Glycosylation811N-linked (GlcNAc...) Potential
Glycosylation1741N-linked (GlcNAc...) Potential
Glycosylation2691N-linked (GlcNAc...) Potential
Glycosylation3691N-linked (GlcNAc...) Potential
Glycosylation4021N-linked (GlcNAc...) Potential
Glycosylation4121N-linked (GlcNAc...) Potential
Glycosylation4211N-linked (GlcNAc...) Potential
Glycosylation4261N-linked (GlcNAc...) Potential
Glycosylation4341N-linked (GlcNAc...) Potential
Disulfide bond41 ↔ 73 By similarity
Disulfide bond120 ↔ 150 By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q99172-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 1720331848F46594

FASTA46951,737
        10         20         30         40         50         60 
MFPFFFALFF SSTDVLAASP SYNGLGLTPQ MGWDNWNSFG CSVKEELLLG TAEKIVKLGL 

        70         80         90        100        110        120 
KDLGYNYIIL DDCWSSGRSS NGSLLADDSK FPHGMKYVAE QLHNSQLKFG MYSSAGEYTC 

       130        140        150        160        170        180 
AGYAGSLGYE DMDAATFASW DVDYLKYDNC YNKGEFGTPE ISYKRYKAMS DALNKTGRPI 

       190        200        210        220        230        240 
FYSLCNWGQD LTFYWGSAIS NSWRMSGDVY PQFDRPDSRC PCSGDEYDCS YPGFHCSIMN 

       250        260        270        280        290        300 
ILNKAAPMGQ NAAPGGWNDL DMLEVGVGNM SDSEEVAHFS MWAIVKSPLI IGADIDDLKD 

       310        320        330        340        350        360 
SSLSVYSNPA VIAINQDVLG TPATRIWKYH VSDKDQYGEG EIQLWSGPLD NGDHVVALLN 

       370        380        390        400        410        420 
GGNNERSMNA SWNDIFIDYL ADSDELSNTW GLYDLWARRM SNATAASILS GNMTSAGLNY 

       430        440        450        460 
NVTQLNYTAG IARNDSRLFG DRVVELSKGE SLTATVPGHG VALFRLRPE

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References

[1]"Characterization of MEL genes in the genus Zygosaccharomyces."
Turakainen H., Hankaanpaa M., Korhola M., Aho S.
Yeast 10:733-745(1994) [PubMed: 7975892] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

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Cross-references

Sequence databases

L24957 Genomic DNA. Translation: AAA35280.1.
PIRS45453.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGH27. Glycoside Hydrolase Family 27.

Enzyme and pathway databases

BRENDA3.2.1.22. 306512.

Family and domain databases

InterProIPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_GHD.
IPR006215. Glyco_hydro_melibiase.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PfamPF02065. Melibiase. 1 hit.
[Graphical view]
PRINTSPR00740. GLHYDRLASE27.
PR00748. MELIBIASE.
ProDomPD002572. Glyco_hydro_GHD. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMEL_ZYGCI
AccessionPrimary (citable) accession number: Q99172
Entry history
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: November 1, 1996
Last modified: June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents