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Protein

78 kDa glucose-regulated protein homolog

Gene

KAR2

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Is required for secretory polypeptide translocation. May physically associate with SEC63 protein in the endoplasmic reticulum and this interaction may be regulated by ATP hydrolysis (By similarity).By similarity

GO - Molecular functioni

  1. ATPase activity Source: EnsemblFungi
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' posttranslational protein folding Source: EnsemblFungi
  2. ER-associated ubiquitin-dependent protein catabolic process Source: EnsemblFungi
  3. karyogamy involved in conjugation with cellular fusion Source: EnsemblFungi
  4. posttranslational protein targeting to membrane, translocation Source: EnsemblFungi
  5. response to unfolded protein Source: UniProtKB
  6. SRP-dependent cotranslational protein targeting to membrane, translocation Source: EnsemblFungi
Complete GO annotation...

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
78 kDa glucose-regulated protein homolog
Short name:
GRP-78
Alternative name(s):
Immunoglobulin heavy chain-binding protein homolog
Short name:
BiP
Gene namesi
Name:KAR2
Ordered Locus Names:YALI0E13706g
OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Taxonomic identifieri284591 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
ProteomesiUP000001300 Componenti: Chromosome E

Subcellular locationi

GO - Cellular componenti

  1. Golgi apparatus Source: EnsemblFungi
  2. luminal surveillance complex Source: EnsemblFungi
  3. nuclear membrane Source: EnsemblFungi
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 67064478 kDa glucose-regulated protein homologPRO_0000013586Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi464 – 4641N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ99170.

Interactioni

Protein-protein interaction databases

IntActiQ99170. 1 interaction.
MINTiMINT-1540646.
STRINGi4952.Q99170.

Structurei

3D structure databases

ProteinModelPortaliQ99170.
SMRiQ99170. Positions 37-576.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi667 – 6704Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0443.
HOGENOMiHOG000228135.
InParanoidiQ99170.
KOiK09490.
OMAiPTRTIFD.
OrthoDBiEOG728916.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99170-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFSMPSWGV VFYALLVCLL PFLSKAGVQA DDVDSYGTVI GIDLGTTYSC
60 70 80 90 100
VGVMKGGRVE ILANDQGSRI TPSYVAFTED ERLVGDAAKN QAANNPFNTI
110 120 130 140 150
FDIKRLIGLK YKDESVQRDI KHFPYKVKNK DGKPVVVVET KGEKKTYTPE
160 170 180 190 200
EISAMILTKM KDIAQDYLGK KVTHAVVTVP AYFNDAQRQA TKDAGIIAGL
210 220 230 240 250
NVLRIVNEPT AAAIAYGLDH TDDEKQIVVY DLGGGTFDVS LLSIESGVFE
260 270 280 290 300
VLATAGDTHL GGEDFDYRVI KHFVKQYNKK HDVDITKNAK TIGKLKREVE
310 320 330 340 350
KAKRTLSSQM STRIEIESFF DGEDFSETLT RAKFEELNID LFKRTLKPVE
360 370 380 390 400
QVLKDSGVKK EDVHDIVLVG GSTRIPKVQE LLEKFFDGKK ASKGINPDEA
410 420 430 440 450
VAYGAAVQAG VLSGEDGVED IVLLDVNPLT LGIETTGGVM TKLINRNTNI
460 470 480 490 500
PTKKSQIFST AVDNQSTVLI QVFEGERTMS KDNNLLGKFE LKGIPPAPRG
510 520 530 540 550
VPQIEVTFEL DANGILRVTA HDKGTGKSET ITITNDKGRL SKDEIERMVE
560 570 580 590 600
EAERFAEEDA LIRETIEAKN SLENYAHSLR NQVADKSGLG GKISADDKEA
610 620 630 640 650
LNDAVTETLE WLEANSVSAT KEDFEEKKEA LSAIAYPITS KIYEGGEGGD
660 670
ESNDGGFYAD DDEAPFHDEL
Length:670
Mass (Da):73,524
Last modified:October 31, 1997 - v1
Checksum:i2C2DFB436BCC9C0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U63136 Genomic DNA. Translation: AAC49736.1.
CR382131 Genomic DNA. Translation: CAG79506.1.
RefSeqiXP_503913.1. XM_503913.1.

Genome annotation databases

EnsemblFungiiCAG79506; CAG79506; YALI0_E13706g.
GeneIDi2912786.
KEGGiyli:YALI0E13706g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U63136 Genomic DNA. Translation: AAC49736.1.
CR382131 Genomic DNA. Translation: CAG79506.1.
RefSeqiXP_503913.1. XM_503913.1.

3D structure databases

ProteinModelPortaliQ99170.
SMRiQ99170. Positions 37-576.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99170. 1 interaction.
MINTiMINT-1540646.
STRINGi4952.Q99170.

Proteomic databases

PRIDEiQ99170.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG79506; CAG79506; YALI0_E13706g.
GeneIDi2912786.
KEGGiyli:YALI0E13706g.

Phylogenomic databases

eggNOGiCOG0443.
HOGENOMiHOG000228135.
InParanoidiQ99170.
KOiK09490.
OMAiPTRTIFD.
OrthoDBiEOG728916.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Yarrowia lipolytica SRP54 homolog and translocation of Kar2p."
    Lee I.H., Ogrydziak D.M.
    Yeast 13:499-513(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 32338 / CX-161-1B.
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CLIB 122 / E 150.

Entry informationi

Entry nameiGRP78_YARLI
AccessioniPrimary (citable) accession number: Q99170
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1997
Last sequence update: October 31, 1997
Last modified: March 31, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.