Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q99158

- SIL1_YARLI

UniProt

Q99158 - SIL1_YARLI

Protein

Nucleotide exchange factor SIL1

Gene

SIL1

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 2 (11 Oct 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Required for protein translocation and folding in the endoplasmic reticulum (ER). Probably functions as a nucleotide exchange factor for the ER lumenal chaperone KAR2. It is not required for viability but is essential for optimal growth at elevated temperatures.2 Publications

    GO - Biological processi

    1. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Protein transport, Translocation, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleotide exchange factor SIL1
    Alternative name(s):
    Protein SLS1
    Gene namesi
    Name:SIL1
    Synonyms:SLS1
    Ordered Locus Names:YALI0E32703g
    OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
    Taxonomic identifieri284591 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
    ProteomesiUP000001300: Chromosome E

    Subcellular locationi

    Endoplasmic reticulum lumen 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi382 – 3876FLEWLD → YY: Abrogates interaction with KAR2. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 426409Nucleotide exchange factor SIL1PRO_0000032596Add
    BLAST

    Expressioni

    Inductioni

    By the unfolded protein response (UPR).1 Publication

    Interactioni

    Subunit structurei

    Interacts with KAR2.1 Publication

    Protein-protein interaction databases

    IntActiQ99158. 1 interaction.
    MINTiMINT-1540659.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi423 – 4264Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the SIL1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG284817.
    KOiK14001.
    OMAiDSAHEIY.
    OrthoDBiEOG7CK3GS.

    Family and domain databases

    Gene3Di1.25.10.10. 1 hit.
    InterProiIPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q99158-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFSKTLLLA LVAGALAKGE DEICRVEKNS GKEICYPKVF VPTEEWQVVW    50
    PDQVIPAGLH VRMDYENGVK EAKINDPNEE VEGVAVAVGE EVPEGEVVIE 100
    DLTEENGDEG ISANEKVQRA IEKAIKEKRI KEGHKPNPNI PESDHQTFSD 150
    AVAALRDYKV NGQAAMLPIA LSQLEELSHE IDFGIALSDV DPLNALLQIL 200
    EDAKVDVESK IMAARTIGAS LRNNPHALDK VINSKVDLVK SLLDDLAQSS 250
    KEKADKLSSS LVYALSAVLK TPETVTRFVD LHGGDTLRQL YETGSDDVKG 300
    RVSTLIEDVL ATPDLHNDFS SITGAVKKRS ANWWEDELKE WSGVFQRSLP 350
    SKLSSKVKSK VYTSLAAIRR NFRESVDVSE EFLEWLDHPK KAAAEIGDDL 400
    VKLIKQDRGE LWGNAKARKY DARDEL 426
    Length:426
    Mass (Da):47,174
    Last modified:October 11, 2004 - v2
    Checksum:iEB475653FF712BF1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti323 – 3231T → K in CAA90516. (PubMed:8662639)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z50154 Genomic DNA. Translation: CAA90516.1.
    CR382131 Genomic DNA. Translation: CAG80301.1.
    PIRiS58132.
    RefSeqiXP_504697.1. XM_504697.1.

    Genome annotation databases

    EnsemblFungiiCAG80301; CAG80301; YALI0_E32703g.
    GeneIDi2911792.
    KEGGiyli:YALI0E32703g.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z50154 Genomic DNA. Translation: CAA90516.1 .
    CR382131 Genomic DNA. Translation: CAG80301.1 .
    PIRi S58132.
    RefSeqi XP_504697.1. XM_504697.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q99158. 1 interaction.
    MINTi MINT-1540659.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CAG80301 ; CAG80301 ; YALI0_E32703g .
    GeneIDi 2911792.
    KEGGi yli:YALI0E32703g.

    Phylogenomic databases

    eggNOGi NOG284817.
    KOi K14001.
    OMAi DSAHEIY.
    OrthoDBi EOG7CK3GS.

    Family and domain databases

    Gene3Di 1.25.10.10. 1 hit.
    InterProi IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sls1p, an endoplasmic reticulum component, is involved in the protein translocation process in the yeast Yarrowia lipolytica."
      Boisrame A., Beckerich J.-M., Gaillardin C.
      J. Biol. Chem. 271:11668-11675(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
      Strain: ATCC 20460 / W29 / CBS 7504 / IFP29.
    2. "Genome evolution in yeasts."
      Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
      , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
      Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CLIB 122 / E 150.
    3. "Interaction of Kar2p and Sls1p is required for efficient co-translational translocation of secreted proteins in the yeast Yarrowia lipolytica."
      Boisrame A., Kabani M., Beckerich J.-M., Hartmann E., Gaillardin C.
      J. Biol. Chem. 273:30903-30908(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH KAR2, MUTAGENESIS OF 382-PHE--ASP-387.

    Entry informationi

    Entry nameiSIL1_YARLI
    AccessioniPrimary (citable) accession number: Q99158
    Secondary accession number(s): Q6C3R5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 76 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3