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Q99158

- SIL1_YARLI

UniProt

Q99158 - SIL1_YARLI

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Protein

Nucleotide exchange factor SIL1

Gene

SIL1

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Required for protein translocation and folding in the endoplasmic reticulum (ER). Probably functions as a nucleotide exchange factor for the ER lumenal chaperone KAR2. It is not required for viability but is essential for optimal growth at elevated temperatures.2 Publications

GO - Biological processi

  1. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleotide exchange factor SIL1
Alternative name(s):
Protein SLS1
Gene namesi
Name:SIL1
Synonyms:SLS1
Ordered Locus Names:YALI0E32703g
OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Taxonomic identifieri284591 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
ProteomesiUP000001300: Chromosome E

Subcellular locationi

Endoplasmic reticulum lumen 1 PublicationPROSITE-ProRule annotation

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi382 – 3876FLEWLD → YY: Abrogates interaction with KAR2. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Chaini18 – 426409Nucleotide exchange factor SIL1PRO_0000032596Add
BLAST

Expressioni

Inductioni

By the unfolded protein response (UPR).1 Publication

Interactioni

Subunit structurei

Interacts with KAR2.1 Publication

Protein-protein interaction databases

IntActiQ99158. 1 interaction.
MINTiMINT-1540659.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi423 – 4264Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the SIL1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG284817.
InParanoidiQ99158.
KOiK14001.
OMAiDSAHEIY.
OrthoDBiEOG7CK3GS.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99158-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFSKTLLLA LVAGALAKGE DEICRVEKNS GKEICYPKVF VPTEEWQVVW
60 70 80 90 100
PDQVIPAGLH VRMDYENGVK EAKINDPNEE VEGVAVAVGE EVPEGEVVIE
110 120 130 140 150
DLTEENGDEG ISANEKVQRA IEKAIKEKRI KEGHKPNPNI PESDHQTFSD
160 170 180 190 200
AVAALRDYKV NGQAAMLPIA LSQLEELSHE IDFGIALSDV DPLNALLQIL
210 220 230 240 250
EDAKVDVESK IMAARTIGAS LRNNPHALDK VINSKVDLVK SLLDDLAQSS
260 270 280 290 300
KEKADKLSSS LVYALSAVLK TPETVTRFVD LHGGDTLRQL YETGSDDVKG
310 320 330 340 350
RVSTLIEDVL ATPDLHNDFS SITGAVKKRS ANWWEDELKE WSGVFQRSLP
360 370 380 390 400
SKLSSKVKSK VYTSLAAIRR NFRESVDVSE EFLEWLDHPK KAAAEIGDDL
410 420
VKLIKQDRGE LWGNAKARKY DARDEL
Length:426
Mass (Da):47,174
Last modified:October 11, 2004 - v2
Checksum:iEB475653FF712BF1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti323 – 3231T → K in CAA90516. (PubMed:8662639)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z50154 Genomic DNA. Translation: CAA90516.1.
CR382131 Genomic DNA. Translation: CAG80301.1.
PIRiS58132.
RefSeqiXP_504697.1. XM_504697.1.

Genome annotation databases

EnsemblFungiiCAG80301; CAG80301; YALI0_E32703g.
GeneIDi2911792.
KEGGiyli:YALI0E32703g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z50154 Genomic DNA. Translation: CAA90516.1 .
CR382131 Genomic DNA. Translation: CAG80301.1 .
PIRi S58132.
RefSeqi XP_504697.1. XM_504697.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q99158. 1 interaction.
MINTi MINT-1540659.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CAG80301 ; CAG80301 ; YALI0_E32703g .
GeneIDi 2911792.
KEGGi yli:YALI0E32703g.

Phylogenomic databases

eggNOGi NOG284817.
InParanoidi Q99158.
KOi K14001.
OMAi DSAHEIY.
OrthoDBi EOG7CK3GS.

Family and domain databases

Gene3Di 1.25.10.10. 1 hit.
InterProi IPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS00014. ER_TARGET. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sls1p, an endoplasmic reticulum component, is involved in the protein translocation process in the yeast Yarrowia lipolytica."
    Boisrame A., Beckerich J.-M., Gaillardin C.
    J. Biol. Chem. 271:11668-11675(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
    Strain: ATCC 20460 / W29 / CBS 7504 / IFP29.
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CLIB 122 / E 150.
  3. "Interaction of Kar2p and Sls1p is required for efficient co-translational translocation of secreted proteins in the yeast Yarrowia lipolytica."
    Boisrame A., Kabani M., Beckerich J.-M., Hartmann E., Gaillardin C.
    J. Biol. Chem. 273:30903-30908(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH KAR2, MUTAGENESIS OF 382-PHE--ASP-387.

Entry informationi

Entry nameiSIL1_YARLI
AccessioniPrimary (citable) accession number: Q99158
Secondary accession number(s): Q6C3R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 11, 2004
Last modified: October 29, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3