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Q99158 (SIL1_YARLI) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleotide exchange factor SIL1
Alternative name(s):
Protein SLS1
Gene names
Name:SIL1
Synonyms:SLS1
Ordered Locus Names:YALI0E32703g
OrganismYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) [Reference proteome]
Taxonomic identifier284591 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia

Protein attributes

Sequence length426 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for protein translocation and folding in the endoplasmic reticulum (ER). Probably functions as a nucleotide exchange factor for the ER lumenal chaperone KAR2. It is not required for viability but is essential for optimal growth at elevated temperatures. Ref.1 Ref.3

Subunit structure

Interacts with KAR2. Ref.3

Subcellular location

Endoplasmic reticulum lumen Ref.1.

Induction

By the unfolded protein response (UPR). Ref.1

Sequence similarities

Belongs to the SIL1 family.

Ontologies

Keywords
   Biological processProtein transport
Translocation
Transport
   Cellular componentEndoplasmic reticulum
   DomainSignal
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentendoplasmic reticulum lumen

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 426409Nucleotide exchange factor SIL1
PRO_0000032596

Regions

Motif423 – 4264Prevents secretion from ER Potential

Experimental info

Mutagenesis382 – 3876FLEWLD → YY: Abrogates interaction with KAR2. Ref.3
Sequence conflict3231T → K in CAA90516. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q99158 [UniParc].

Last modified October 11, 2004. Version 2.
Checksum: EB475653FF712BF1

FASTA42647,174
        10         20         30         40         50         60 
MKFSKTLLLA LVAGALAKGE DEICRVEKNS GKEICYPKVF VPTEEWQVVW PDQVIPAGLH 

        70         80         90        100        110        120 
VRMDYENGVK EAKINDPNEE VEGVAVAVGE EVPEGEVVIE DLTEENGDEG ISANEKVQRA 

       130        140        150        160        170        180 
IEKAIKEKRI KEGHKPNPNI PESDHQTFSD AVAALRDYKV NGQAAMLPIA LSQLEELSHE 

       190        200        210        220        230        240 
IDFGIALSDV DPLNALLQIL EDAKVDVESK IMAARTIGAS LRNNPHALDK VINSKVDLVK 

       250        260        270        280        290        300 
SLLDDLAQSS KEKADKLSSS LVYALSAVLK TPETVTRFVD LHGGDTLRQL YETGSDDVKG 

       310        320        330        340        350        360 
RVSTLIEDVL ATPDLHNDFS SITGAVKKRS ANWWEDELKE WSGVFQRSLP SKLSSKVKSK 

       370        380        390        400        410        420 
VYTSLAAIRR NFRESVDVSE EFLEWLDHPK KAAAEIGDDL VKLIKQDRGE LWGNAKARKY 


DARDEL 

« Hide

References

« Hide 'large scale' references
[1]"Sls1p, an endoplasmic reticulum component, is involved in the protein translocation process in the yeast Yarrowia lipolytica."
Boisrame A., Beckerich J.-M., Gaillardin C.
J. Biol. Chem. 271:11668-11675(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
Strain: ATCC 20460 / W29 / CBS 7504 / IFP29.
[2]"Genome evolution in yeasts."
Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. expand/collapse author list , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CLIB 122 / E 150.
[3]"Interaction of Kar2p and Sls1p is required for efficient co-translational translocation of secreted proteins in the yeast Yarrowia lipolytica."
Boisrame A., Kabani M., Beckerich J.-M., Hartmann E., Gaillardin C.
J. Biol. Chem. 273:30903-30908(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH KAR2, MUTAGENESIS OF 382-PHE--ASP-387.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z50154 Genomic DNA. Translation: CAA90516.1.
CR382131 Genomic DNA. Translation: CAG80301.1.
PIRS58132.
RefSeqXP_504697.1. XM_504697.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ99158. 1 interaction.
MINTMINT-1540659.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCAG80301; CAG80301; YALI0_E32703g.
GeneID2911792.
KEGGyli:YALI0E32703g.

Phylogenomic databases

eggNOGNOG284817.
KOK14001.
OMADSAHEIY.
OrthoDBEOG7CK3GS.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
PROSITEPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSIL1_YARLI
AccessionPrimary (citable) accession number: Q99158
Secondary accession number(s): Q6C3R5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 11, 2004
Last modified: November 13, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families