Q99158 (SIL1_YARLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleotide exchange factor SIL1

Alternative name(s):
Protein SLS1

Gene names

Name:	SIL1
Synonyms:	SLS1
Ordered Locus Names:	YALI0E32703g

Organism

Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) [Reference proteome]

Taxonomic identifier

284591 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia › Yarrowia lipolytica

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for protein translocation and folding in the endoplasmic reticulum (ER). Probably functions as a nucleotide exchange factor for the ER lumenal chaperone KAR2. It is not required for viability but is essential for optimal growth at elevated temperatures. Ref.1 Ref.3
Subunit structure	Interacts with KAR2. Ref.3
Subcellular location	Endoplasmic reticulum lumen Ref.1.
Induction	By the unfolded protein response (UPR). Ref.1
Sequence similarities	Belongs to the SIL1 family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Endoplasmic reticulum
Domain	Signal
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 426	409	Nucleotide exchange factor SIL1		PRO_0000032596
Regions
	Motif	423 – 426	4	Prevents secretion from ER Potential
Experimental info
	Mutagenesis	382 – 387	6	FLEWLD → YY: Abrogates interaction with KAR2. Ref.3
	Sequence conflict	323	1	T → K in CAA90516. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q99158 [UniParc]. Last modified October 11, 2004. Version 2. Checksum: EB475653FF712BF1	FASTA	426	47,174
	10 20 30 40 50 60 MKFSKTLLLA LVAGALAKGE DEICRVEKNS GKEICYPKVF VPTEEWQVVW PDQVIPAGLH 70 80 90 100 110 120 VRMDYENGVK EAKINDPNEE VEGVAVAVGE EVPEGEVVIE DLTEENGDEG ISANEKVQRA 130 140 150 160 170 180 IEKAIKEKRI KEGHKPNPNI PESDHQTFSD AVAALRDYKV NGQAAMLPIA LSQLEELSHE 190 200 210 220 230 240 IDFGIALSDV DPLNALLQIL EDAKVDVESK IMAARTIGAS LRNNPHALDK VINSKVDLVK 250 260 270 280 290 300 SLLDDLAQSS KEKADKLSSS LVYALSAVLK TPETVTRFVD LHGGDTLRQL YETGSDDVKG 310 320 330 340 350 360 RVSTLIEDVL ATPDLHNDFS SITGAVKKRS ANWWEDELKE WSGVFQRSLP SKLSSKVKSK 370 380 390 400 410 420 VYTSLAAIRR NFRESVDVSE EFLEWLDHPK KAAAEIGDDL VKLIKQDRGE LWGNAKARKY DARDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sls1p, an endoplasmic reticulum component, is involved in the protein translocation process in the yeast Yarrowia lipolytica." Boisrame A., Beckerich J.-M., Gaillardin C. J. Biol. Chem. 271:11668-11675(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INDUCTION. Strain: ATCC 20460 / W29 / CBS 7504 / IFP29.
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.
[3]	"Interaction of Kar2p and Sls1p is required for efficient co-translational translocation of secreted proteins in the yeast Yarrowia lipolytica." Boisrame A., Kabani M., Beckerich J.-M., Hartmann E., Gaillardin C. J. Biol. Chem. 273:30903-30908(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH KAR2, MUTAGENESIS OF 382-PHE--ASP-387.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z50154 Genomic DNA. Translation: CAA90516.1. CR382131 Genomic DNA. Translation: CAG80301.1.
PIR	S58132.
RefSeq	XP_504697.1. XM_504697.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2911792.
KEGG	yli:YALI0E32703g.
Phylogenomic databases
eggNOG	NOG284817.
KO	K14001.
OrthoDB	EOG40312K.
Family and domain databases
Gene3D	1.25.10.10. 1 hit.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SIL1_YARLI

Accession

Primary (citable) accession number: Q99158
Secondary accession number(s): Q6C3R5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program