Q99156 (LIP1_YARLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipase 1
EC=3.1.1.3

Gene names

Name:	LIP1
Ordered Locus Names:	YALI0E10659g

Organism

Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)

Taxonomic identifier

284591 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Dipodascaceae › Yarrowia

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Sequence similarities	Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Keywords
Biological process	Lipid degradation
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?		Potential
	Chain	? – 486		Lipase 1		PRO_0000008627
Sites
	Active site	193	1	Acyl-ester intermediate By similarity
	Active site	303	1	Charge relay system By similarity
	Active site	392	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	332	1	N-linked (GlcNAc...) Potential
	Disulfide bond	58 ↔ 82		By similarity
Experimental info
	Sequence conflict	31 – 32	2	IP → RR in CAA90323. Ref.1
	Sequence conflict	237	1	N → D in CAA90323. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q99156 [UniParc]. Last modified October 11, 2004. Version 2. Checksum: 497A796EF64D7EA7	FASTA	486	55,380
	10 20 30 40 50 60 MSVTSTSLNG TFNGISEDGI EIFKGIKYAN IPYRWAYAER IDDYDNGVFD CTQEGMACPQ 70 80 90 100 110 120 VLPFDYNIEK GPKEMPFDEF ECSNLMITRP QGATNLPVFV WIHGGGNLAG NGYCSDHNPV 130 140 150 160 170 180 PFVKHSIVAG RPVLHVMIEY RLSAFGYLAV PDTNGNWVGN WGARDQYTAL QWISKHIVEF 190 200 210 220 230 240 GGDPSQITIG GESAGSIGLH ALMVHESMKP KEECIIHNVI LSSGTMDRMG TGTISENAFK 250 260 270 280 290 300 PIYDGIKTLV GDINTCSADE LLEAQIKAGL DLGFYLQDDF FPPDWRNVRF KVSRVLLSDV 310 320 330 340 350 360 IVDGTNFKNK INPAVRVTPE NDFDHKVFKL YNISTEDTWE DYHYKMMLFK GDETFIRGNQ 370 380 390 400 410 420 QLELLFEQEN IPVWRQLFDQ IHPNDPSRLC HHAVDLYYMW DNWEMPEDKH AVARQYQDTL 430 440 450 460 470 480 TKFVYGQDPW PVDKLHYVHD NQFEILDKSQ FGDFRNVPAL KFLLGFSAEE LGELTKKYTG EGHYTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Gonzalez F. Thesis (1996), University of Salamanca, Spain Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 90811 / CLIB 163 / JM12.
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CLIB 122 / E 150.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z50020 Genomic DNA. Translation: CAA90323.1. CR382131 Genomic DNA. Translation: CAG79381.1.
RefSeq	XP_503790.1. XM_503790.1.
3D structure databases
ProteinModelPortal	Q99156.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2912789.
GenomeReviews	Gene locus YALI0E10659g in contig CR382131_GR.
KEGG	yli:YALI0E10659g.
Phylogenomic databases
eggNOG	fuNOG11170.
HOGENOM	HBG272480.
OrthoDB	EOG4V9XZX.
Family and domain databases
InterPro	IPR002018. CarbesteraseB. IPR019826. Carboxylesterase_B_AS. [Graphical view]
Pfam	PF00135. COesterase. 1 hit. [Graphical view]
PROSITE	PS00122. CARBOXYLESTERASE_B_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIP1_YARLI

Accession

Primary (citable) accession number: Q99156
Secondary accession number(s): Q6C6C2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 11, 2004
Last modified:	September 21, 2011
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program