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Protein

Bifunctional purine biosynthetic protein ADE1

Gene

ADE1

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi291 – 2911ManganeseBy similarity
Metal bindingi293 – 2931ManganeseBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi140 – 20162ATPBy similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoribosylamine-glycine ligase activity Source: UniProtKB-EC
  4. phosphoribosylformylglycinamidine cyclo-ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125.
UPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthetic protein ADE1
Including the following 2 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene namesi
Name:ADE1
Ordered Locus Names:YALI0F21010g
OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Taxonomic identifieri284591 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
ProteomesiUP000001300: Chromosome F

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 788788Bifunctional purine biosynthetic protein ADE1PRO_0000074940Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi4952.Q99148.

Structurei

3D structure databases

ProteinModelPortaliQ99148.
SMRiQ99148. Positions 439-782.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini114 – 323210ATP-graspAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 432432GARSAdd
BLAST
Regioni442 – 752311AIRSAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the C-terminal section; belongs to the AIR synthase family.Curated
Contains 1 ATP-grasp domain.Curated

Phylogenomic databases

eggNOGiCOG0151.
HOGENOMiHOG000030315.
InParanoidiQ99148.
KOiK11788.
OMAiGMLFTGF.
OrthoDBiEOG7Q8CXX.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00138. GARS.
MF_00741. AIRS.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99148-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLRILLVGN GGREHALAWK LAQSPLVERI FVAPGNGGTD NPQGKIENIA
60 70 80 90 100
IGSSQKDFAK LVEFAQSKDV GLVIPGPEQP LVEGIETHFR KVGIPVFGPS
110 120 130 140 150
EKAAVMEGSK TFSKDFMKKH NIPTAAFENF TDYNKAVDYV KKVGHRVVIK
160 170 180 190 200
ASGLAAGKGV LIPTSTEEAI AAVKEVMQDK AFGEAGDEVV IEEFLEGDEL
210 220 230 240 250
SILAFSDGYT VVDMPPAQDH KRIGDGDQGL NTGGMGAYCP APIGTPALLQ
260 270 280 290 300
EIKESILQPT IDGMRRDGIP FVGMLFTGIM LTPDGKPKVL EYNVRFGDPE
310 320 330 340 350
TQTILPLLSD DTDLAEVMLA CVERRLDAVT LRVKPDAHAV TVVMSAGGYP
360 370 380 390 400
ESYKKGDAIT VGGLPEQTYI FHAGTASENG QVITAGGRVI ASTAVAPTLK
410 420 430 440 450
DAVAQAYKGA DAVEFNGKYN RKDIAYKAFR DAEKTSGGIT YAQAGVSIDN
460 470 480 490 500
GNKLVQQIKE KVKSTARPGT DSVIGGFGGL FDLKAAGFRD PLLVGATDGV
510 520 530 540 550
GTKLKIAQSI DKHDTVGIDL VAMNVNDLVV QGAEPLVFLD YYATGKLDVN
560 570 580 590 600
AAAAFVGGVA DGCIQAGCAL IGGETAEMPG IYYGNDYDAN GTSIGAVERD
610 620 630 640 650
AVLPRMDEIA KGDAILGLAS SGVHSNGFSL VRKIIEHAGL TYTDACPWDQ
660 670 680 690 700
SKSLGEALLT PTRIYVKQLL PVINAKLTSA LAHITGGGLV ENIPRILPEN
710 720 730 740 750
YSAKIDVSTW PLPPVFQWLG KAGNVPKEDI SKTLNMGIGM ILVVKQEKVA
760 770 780
EVTQLLEKVG EKVYQIGEIV PDNDVDEKTV LINTENWY
Length:788
Mass (Da):83,759
Last modified:November 1, 1997 - v1
Checksum:i6EC170ECB27AFCF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40565 Genomic DNA. Translation: AAA85393.1.
CR382132 Genomic DNA. Translation: CAG78498.1.
RefSeqiXP_505689.1. XM_505689.1.

Genome annotation databases

EnsemblFungiiCAG78498; CAG78498; YALI0_F21010g.
GeneIDi2907698.
KEGGiyli:YALI0F21010g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40565 Genomic DNA. Translation: AAA85393.1.
CR382132 Genomic DNA. Translation: CAG78498.1.
RefSeqiXP_505689.1. XM_505689.1.

3D structure databases

ProteinModelPortaliQ99148.
SMRiQ99148. Positions 439-782.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4952.Q99148.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG78498; CAG78498; YALI0_F21010g.
GeneIDi2907698.
KEGGiyli:YALI0F21010g.

Phylogenomic databases

eggNOGiCOG0151.
HOGENOMiHOG000030315.
InParanoidiQ99148.
KOiK11788.
OMAiGMLFTGF.
OrthoDBiEOG7Q8CXX.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125.
UPA00074; UER00129.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00138. GARS.
MF_00741. AIRS.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Strick C.A., James L.C., Cole K.E., Elsenboss L.A.
    Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CLIB 122 / E 150.

Entry informationi

Entry nameiPUR2_YARLI
AccessioniPrimary (citable) accession number: Q99148
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.