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Q99148 (PUR2_YARLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional purine biosynthetic protein ADE1

Including the following 2 domains:

  1. Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
    Alternative name(s):
    Glycinamide ribonucleotide synthetase
    Short name=GARS
    Phosphoribosylglycinamide synthetase
  2. Phosphoribosylformylglycinamidine cyclo-ligase
    EC=6.3.3.1
    Alternative name(s):
    AIR synthase
    Short name=AIRS
    Phosphoribosyl-aminoimidazole synthetase
Gene names
Name:ADE1
Ordered Locus Names:YALI0F21010g
OrganismYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) [Reference proteome]
Taxonomic identifier284591 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia

Protein attributes

Sequence length788 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00138

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00138

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.

Sequence similarities

In the N-terminal section; belongs to the GARS family.

In the C-terminal section; belongs to the AIR synthase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 788788Bifunctional purine biosynthetic protein ADE1 HAMAP-Rule MF_00138
PRO_0000074940

Regions

Domain114 – 323210ATP-grasp
Nucleotide binding140 – 20162ATP By similarity
Region1 – 432432GARS HAMAP-Rule MF_00138
Region442 – 752311AIRS HAMAP-Rule MF_00138

Sites

Metal binding2911Manganese By similarity
Metal binding2931Manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q99148 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 6EC170ECB27AFCF4

FASTA78883,759
        10         20         30         40         50         60 
MSLRILLVGN GGREHALAWK LAQSPLVERI FVAPGNGGTD NPQGKIENIA IGSSQKDFAK 

        70         80         90        100        110        120 
LVEFAQSKDV GLVIPGPEQP LVEGIETHFR KVGIPVFGPS EKAAVMEGSK TFSKDFMKKH 

       130        140        150        160        170        180 
NIPTAAFENF TDYNKAVDYV KKVGHRVVIK ASGLAAGKGV LIPTSTEEAI AAVKEVMQDK 

       190        200        210        220        230        240 
AFGEAGDEVV IEEFLEGDEL SILAFSDGYT VVDMPPAQDH KRIGDGDQGL NTGGMGAYCP 

       250        260        270        280        290        300 
APIGTPALLQ EIKESILQPT IDGMRRDGIP FVGMLFTGIM LTPDGKPKVL EYNVRFGDPE 

       310        320        330        340        350        360 
TQTILPLLSD DTDLAEVMLA CVERRLDAVT LRVKPDAHAV TVVMSAGGYP ESYKKGDAIT 

       370        380        390        400        410        420 
VGGLPEQTYI FHAGTASENG QVITAGGRVI ASTAVAPTLK DAVAQAYKGA DAVEFNGKYN 

       430        440        450        460        470        480 
RKDIAYKAFR DAEKTSGGIT YAQAGVSIDN GNKLVQQIKE KVKSTARPGT DSVIGGFGGL 

       490        500        510        520        530        540 
FDLKAAGFRD PLLVGATDGV GTKLKIAQSI DKHDTVGIDL VAMNVNDLVV QGAEPLVFLD 

       550        560        570        580        590        600 
YYATGKLDVN AAAAFVGGVA DGCIQAGCAL IGGETAEMPG IYYGNDYDAN GTSIGAVERD 

       610        620        630        640        650        660 
AVLPRMDEIA KGDAILGLAS SGVHSNGFSL VRKIIEHAGL TYTDACPWDQ SKSLGEALLT 

       670        680        690        700        710        720 
PTRIYVKQLL PVINAKLTSA LAHITGGGLV ENIPRILPEN YSAKIDVSTW PLPPVFQWLG 

       730        740        750        760        770        780 
KAGNVPKEDI SKTLNMGIGM ILVVKQEKVA EVTQLLEKVG EKVYQIGEIV PDNDVDEKTV 


LINTENWY 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U40565 Genomic DNA. Translation: AAA85393.1.
CR382132 Genomic DNA. Translation: CAG78498.1.
RefSeqXP_505689.1. XM_505689.1.

3D structure databases

ProteinModelPortalQ99148.
SMRQ99148. Positions 439-782.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING4952.Q99148.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCAG78498; CAG78498; YALI0_F21010g.
GeneID2907698.
KEGGyli:YALI0F21010g.

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000030315.
KOK11788.
OMAGMLFTGF.
OrthoDBEOG7Q8CXX.

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.
UPA00074; UER00129.

Family and domain databases

Gene3D3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_YARLI
AccessionPrimary (citable) accession number: Q99148
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways