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Protein

Bifunctional purine biosynthetic protein ADE1

Gene

ADE1

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Bifunctional purine biosynthetic protein ADE1 (ADE1)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (YALI0_B15304g)
  2. Bifunctional purine biosynthetic protein ADE1 (ADE1)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi291ManganeseBy similarity1
Metal bindingi293ManganeseBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi140 – 201ATPBy similarityAdd BLAST62

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase, Multifunctional enzyme
Biological processPurine biosynthesis
LigandATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125
UPA00074; UER00129

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthetic protein ADE1
Including the following 2 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene namesi
Name:ADE1
Ordered Locus Names:YALI0F21010g
OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Taxonomic identifieri284591 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
Proteomesi
  • UP000001300 Componenti: Chromosome F

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000749401 – 788Bifunctional purine biosynthetic protein ADE1Add BLAST788

Proteomic databases

PRIDEiQ99148

Interactioni

Protein-protein interaction databases

STRINGi4952.XP_505689.1

Structurei

3D structure databases

ProteinModelPortaliQ99148
SMRiQ99148
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini114 – 323ATP-graspAdd BLAST210

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 432GARSAdd BLAST432
Regioni442 – 752AIRSAdd BLAST311

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the C-terminal section; belongs to the AIR synthase family.Curated

Phylogenomic databases

HOGENOMiHOG000030315
InParanoidiQ99148
KOiK11788
OMAiLLERHNC
OrthoDBiEOG092C3N9W

Family and domain databases

CDDicd02196 PurM, 1 hit
Gene3Di3.30.1330.10, 1 hit
3.30.1490.20, 1 hit
3.90.600.10, 1 hit
3.90.650.10, 1 hit
HAMAPiMF_00741 AIRS, 1 hit
MF_00138 GARS, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR016185 PreATP-grasp_dom_sf
IPR020561 PRibGlycinamid_synth_ATP-grasp
IPR000115 PRibGlycinamide_synth
IPR020560 PRibGlycinamide_synth_C-dom
IPR037123 PRibGlycinamide_synth_C_sf
IPR020559 PRibGlycinamide_synth_CS
IPR020562 PRibGlycinamide_synth_N
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
IPR004733 PurM_cligase
IPR011054 Rudment_hybrid_motif
PfamiView protein in Pfam
PF00586 AIRS, 1 hit
PF02769 AIRS_C, 1 hit
PF01071 GARS_A, 1 hit
PF02843 GARS_C, 1 hit
PF02844 GARS_N, 1 hit
SMARTiView protein in SMART
SM01210 GARS_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
SSF55326 SSF55326, 1 hit
SSF56042 SSF56042, 1 hit
TIGRFAMsiTIGR00877 purD, 1 hit
TIGR00878 purM, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00184 GARS, 1 hit

Sequencei

Sequence statusi: Complete.

Q99148-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLRILLVGN GGREHALAWK LAQSPLVERI FVAPGNGGTD NPQGKIENIA
60 70 80 90 100
IGSSQKDFAK LVEFAQSKDV GLVIPGPEQP LVEGIETHFR KVGIPVFGPS
110 120 130 140 150
EKAAVMEGSK TFSKDFMKKH NIPTAAFENF TDYNKAVDYV KKVGHRVVIK
160 170 180 190 200
ASGLAAGKGV LIPTSTEEAI AAVKEVMQDK AFGEAGDEVV IEEFLEGDEL
210 220 230 240 250
SILAFSDGYT VVDMPPAQDH KRIGDGDQGL NTGGMGAYCP APIGTPALLQ
260 270 280 290 300
EIKESILQPT IDGMRRDGIP FVGMLFTGIM LTPDGKPKVL EYNVRFGDPE
310 320 330 340 350
TQTILPLLSD DTDLAEVMLA CVERRLDAVT LRVKPDAHAV TVVMSAGGYP
360 370 380 390 400
ESYKKGDAIT VGGLPEQTYI FHAGTASENG QVITAGGRVI ASTAVAPTLK
410 420 430 440 450
DAVAQAYKGA DAVEFNGKYN RKDIAYKAFR DAEKTSGGIT YAQAGVSIDN
460 470 480 490 500
GNKLVQQIKE KVKSTARPGT DSVIGGFGGL FDLKAAGFRD PLLVGATDGV
510 520 530 540 550
GTKLKIAQSI DKHDTVGIDL VAMNVNDLVV QGAEPLVFLD YYATGKLDVN
560 570 580 590 600
AAAAFVGGVA DGCIQAGCAL IGGETAEMPG IYYGNDYDAN GTSIGAVERD
610 620 630 640 650
AVLPRMDEIA KGDAILGLAS SGVHSNGFSL VRKIIEHAGL TYTDACPWDQ
660 670 680 690 700
SKSLGEALLT PTRIYVKQLL PVINAKLTSA LAHITGGGLV ENIPRILPEN
710 720 730 740 750
YSAKIDVSTW PLPPVFQWLG KAGNVPKEDI SKTLNMGIGM ILVVKQEKVA
760 770 780
EVTQLLEKVG EKVYQIGEIV PDNDVDEKTV LINTENWY
Length:788
Mass (Da):83,759
Last modified:November 1, 1997 - v1
Checksum:i6EC170ECB27AFCF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40565 Genomic DNA Translation: AAA85393.1
CR382132 Genomic DNA Translation: CAG78498.1
RefSeqiXP_505689.1, XM_505689.1

Genome annotation databases

EnsemblFungiiCAG78498; CAG78498; YALI0_F21010g
GeneIDi2907698
KEGGiyli:YALI0F21010g

Similar proteinsi

Entry informationi

Entry nameiPUR2_YARLI
AccessioniPrimary (citable) accession number: Q99148
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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