Q99127 (CHS4_USTMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 4
EC=2.4.1.16

Alternative name(s):
Chitin synthase II
Chitin-UDP acetyl-glucosaminyl transferase 4

Gene names

Name:	CHS4
Synonyms:	CHS2
ORF Names:	UM10117, UM00462

Organism

Ustilago maydis (strain 521 / FGSC 9021) (Smut fungus)

Taxonomic identifier

237631 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	942 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note: A constitutive cytoplasmic pool is present that localizes to intracellular microvesicles termed chitosomes. Chitosomes constitute a separate secretory route distinct from the typical secretory pathway and serve as a vehicle for delivering the enzyme to the sites on the cell surface where polysaccharide sythesis takes place. Localizes to septa of yeast-like cells and to the basal septum separating the living tip cell from the vacuolated part in hyphae. Ref.3 Ref.4
Sequence similarities	Belongs to the chitin synthase family. Class I subfamily.
Sequence caution	The sequence CAA61028.1 differs from that shown. Reason: Contaminating sequence at the N-terminus and at the C-terminus. The sequence EAK81373.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 942	942	Chitin synthase 4		PRO_0000193720
Regions
	Transmembrane	641 – 661	21	Helical; Potential
	Transmembrane	674 – 694	21	Helical; Potential
	Transmembrane	709 – 729	21	Helical; Potential
	Transmembrane	755 – 775	21	Helical; Potential
	Transmembrane	783 – 803	21	Helical; Potential
	Transmembrane	885 – 905	21	Helical; Potential
	Transmembrane	909 – 929	21	Helical; Potential
Amino acid modifications
	Glycosylation	604	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	123 – 124	2	PL → RN in CAA61028. Ref.2
	Sequence conflict	126 – 132	7	Missing in CAA61028. Ref.2
	Sequence conflict	152	1	Y → H in CAA61028. Ref.2
	Sequence conflict	360 – 362	3	VGK → GGQ in CAA61028. Ref.2
	Sequence conflict	394	1	Missing in CAA61028. Ref.2
	Sequence conflict	460 – 462	3	GGA → AGS in CAA61028. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q99127 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: E525728BCB8B16F6	FASTA	942	104,902
	10 20 30 40 50 60 MPPRYPFGGG AQSDEAHHQP LERRTTAEAQ GNSFTHGHTA YPTYDMGAEP HSPPHVSYDP 70 80 90 100 110 120 YLPPSTSGDH SGYEMPSANY NDHHRSSYPQ AIHSPPRMTN PYANSQPRQG SADPFDEHDG 130 140 150 160 170 180 DVPLLPRGGN YAYAPASHFD EHGNHVGPVD KYADGDDDVM DEDAVNGQAR GRLLHTQVPH 190 200 210 220 230 240 DDDYMPGGFD PNVLENGQAG GVRFGKIPQR VPRRYKTLKR VELYHGNLVL DCPVPSKLLD 250 260 270 280 290 300 KLNDRESREF THMRYTAATC DPDEFKTERY TLRQVLFDPP RRTELFIVLT MYNEDEELFT 310 320 330 340 350 360 RTMHGVMTNI AHLCTRERSK TWGKEGWKKV VVCIVSDGRL KINSRTLACL AAMGVYQEGV 370 380 390 400 410 420 GKNVVNGKPV TAHIYEYTAQ LSIDPSMHFK GREKGIMPVQ ILFCLKERNQ KKINSHRWFF 430 440 450 460 470 480 NAFGQILQPN ICVLLDVGTM PRPRSIYHLW KAFDINSNVG GACGEIVALK GKFWGALLNP 490 500 510 520 530 540 LVAAQNFEYK MSNILDKPLE SVFGYITVLP GAFSAYRYIA LQNDAHGQGP LCSYFKGETL 550 560 570 580 590 600 HGGQSDADVF TSNMYLAEDR ILCWELVSKR DSAWILHYVK SAQAVTDVPD QVPELISQRR 610 620 630 640 650 660 RWLNGSFFAG IHSIIKFGYI YRSSHSFGRK FALHIEIIYQ TIQLIFSWFG MANFFIAFFI 670 680 690 700 710 720 LTSAMSDKIH ALKVPNLVLS YIYVAFIIFC FLLSMGNRPA GSKAGYTLAM VVFALLTVYM 730 740 750 760 770 780 TGAAIYLAVD SILNATGPNG GGTDALVSNR TFVNIVISLA ATFGIWLIAS IMFLEPMHMV 790 800 810 820 830 840 TSIVQYLLMA PTFVNVISIY AFANINDISW GTKGSDKVMT DLGVVGGSGN NQVEVAIPTE 850 860 870 880 890 900 SKDINDAYDD AIHVLSNKAP KAGPGPVDKE QKQKDYYATV RTNVVLCWSL SNAALVVGIL 910 920 930 940 NISSIGTRTI YMGFLLYSVA GLALFRMMGS TIYLIKRLFS GE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521 / FGSC 9021.
[2]	"Two chitin synthase genes from Ustilago maydis." Xoconostle-Cazares B., Leon-Ramirez C., Ruiz-Herrera J. Microbiology 142:377-387(1996) [PubMed: 8932711] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 113-604. Strain: RK32 / A2B3.
[3]	"Immunolocalization of chitin synthases in the phytopathogenic dimorphic fungus Ustilago maydis." Ruiz-Herrera J., Xoconostle-Cazares B., Reynaga-Pena C.G., Leon-Ramirez C., Carabez-Trejo A. FEMS Yeast Res. 6:999-1009(2006) [PubMed: 17042749] [Abstract] Cited for: SUBCELLULAR LOCATION.
[4]	"Polar localizing class V myosin chitin synthases are essential during early plant infection in the plant pathogenic fungus Ustilago maydis." Weber I., Assmann D., Thines E., Steinberg G. Plant Cell 18:225-242(2006) [PubMed: 16314447] [Abstract] Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACP01000009 Genomic DNA. Translation: EAK81373.1. Sequence problems. X87749 Genomic DNA. Translation: CAA61028.1. Sequence problems.
PIR	S55519.
RefSeq	XP_756609.1. XM_751516.1.
3D structure databases
ProteinModelPortal	Q99127.
ModBase	Search...
Protein-protein interaction databases
STRING	Q99127.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3628503.
KEGG	uma:UM00462.1.
Phylogenomic databases
eggNOG	fuNOG04531.
HOGENOM	HBG327157.
OrthoDB	EOG4GMZ52.
Enzyme and pathway databases
BRENDA	2.4.1.16. 6587.
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR013616. Chitin_synth_N. [Graphical view]
KO	K00698.
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHS4_USTMA

Accession

Primary (citable) accession number: Q99127
Secondary accession number(s): Q4PHF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program