Reviewed, UniProtKB/Swiss-Prot Q99125 (CWLX_BACLI)

Names and origin

Protein names	Recommended name:     Probable N-acetylmuramoyl-L-alanine amidase     EC=3.5.1.28 Alternative name(s):     Cell wall hydrolase     Autolysin     ORFL3
Organism	Bacillus licheniformis
Taxonomic identifier	1402 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 39	39	By similarity
	Chain	40 – 354	315	Probable N-acetylmuramoyl-L-alanine amidase		PRO_0000006456

Sequences

	Sequence		Length	Mass (Da)
	Q99125-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 9A84D197B73B52CD	FASTA	354	38,347
	10 20 30 40 50 60 MVKVINNFVK VNQYDRPGLK LAAVKGIVMH WTATPGASAL NERNYFNGTC IADKRYASAH 70 80 90 100 110 120 YFVDRHEAQH IIPDHEVAYH AHDQNRCYVS FLKPNANTTA LGVEMCVEKD GTIHEETIRN 130 140 150 160 170 180 AAELVADLCK TYGLSADRIV RHYDVTNKGC PTPWVRDAGQ LSAFRKRVDS LLGRKTVSVS 190 200 210 220 230 240 AASTSQTSSS SGIILKKGMS GSHVKKLQTR LVAAGFSLPK YGADGSYGDE TVHAVVSLQK 250 260 270 280 290 300 KAGIKADGIY GPSTEKALSA AEASAAGKSK TWTLPDGIYK VKNPLMKGTA VTQIQTALAA 310 320 330 340 350 LYYYPDKGAK NNGIDGYYGM KTANAVKRFQ LMYGLGADGI YGPKTKAKML SLLK

References

[1]

"Identification of four unique clones encoding 10 kDa proteins from Bacillus that cause phenotypic complementation of a phoA mutant strain of Escherichia coli." Lee J.W.K., Edwards C.W., Hulett F.M. J. Gen. Microbiol. 137:667-677(1991) [PubMed: 2033382] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MC14.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M63942 Genomic DNA. Translation: AAA22888.1.
PIR	D49754.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.1.28. 1017.
Family and domain databases
InterPro	IPR002502. Amidase_2. IPR002477. Peptidoglycan-bd-like. [Graphical view]
Gene3D	G3DSA:3.40.80.10. Amidase_2. 1 hit. G3DSA:1.10.101.10. PGBD_1. 2 hits.
Pfam	PF01510. Amidase_2. 1 hit. PF01471. PG_binding_1. 2 hits. [Graphical view]
SMART	SM00644. Ami_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CWLX_BACLI

Accession

Primary (citable) accession number: Q99125

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)