Reviewed, UniProtKB/Swiss-Prot Q99078 (FUZ7_USTMA)

Names and origin

Protein names

Recommended name:
    Dual specificity protein kinase FUZ7
    EC=2.7.12.2

Gene names

Name:	FUZ7
ORF Names:	UM01514

Organism

Ustilago maydis (Smut fungus) [Complete proteome]

Taxonomic identifier

5270 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Protein kinase that is necessary for a-locus-dependent processes, such as conjugation tube formation, filament formation, and maintenance of filamentous growth, and for a-locus-independent processes, such as tumor induction and teliospore germination.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Dual specificity protein kinase FUZ7		PRO_0000085955
Regions
	Domain	109 – 417	309	Protein kinase
	Nucleotide binding	115 – 123	9	ATP By similarity
Sites
	Active site	231	1	Proton acceptor By similarity
	Binding site	138	1	ATP By similarity
Experimental info
	Sequence conflict	296 – 297	2	EL → DV in AAA62242. Ref.1
	Sequence conflict	356	1	S → T in AAA62242. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q99078-1 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: 7DC3685407E2BBF7	FASTA	435	47,201
	10 20 30 40 50 60 MLSSGAGSSI RKKRNFKGLQ LAESPLASPV DASATTPSHK PGEGSAASNA STIGKSSAVT 70 80 90 100 110 120 PGGSLALPVK NGLDTEPNSG ANYHNKLTQQ LANLELGVEY KLDLKNEDLK TLSELGAGNG 130 140 150 160 170 180 GTVTKVLHEK SGTVMAKKVV FIDAKPSVRK QILRELQILH ECNSPYIVSF YGAYLNEPHI 190 200 210 220 230 240 CMCMEFMQKD SLDGIYKKYG PISPEICGKI AVAVSHGLTY LYDVHRIIHR DVKPSNILVN 250 260 270 280 290 300 GAGQIKICDF GVSGELINSI ADTFVGTSTY MSPERIQGDQ YSVKSDVWSL GVSIIELALG 310 320 330 340 350 360 RFPFAENEED DDSDADNNYT NEDLAGTLSP TKPAPMISLG QNEKQRRRKS KPAGVSLEGS 370 380 390 400 410 420 SHQMSILDLL QHIVNEPPPK LPEGRFPKHM EEFVNLCLLK DPAKRPTPKD LTKHQYVIDA 430 DAAKVDLQAW ADGMK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of fuz7, a Ustilago maydis MEK/MAPKK homolog required for a-locus-dependent and -independent steps in the fungal life cycle." Banuett F., Herskowitz I. Genes Dev. 8:1367-1378(1994) [PubMed: 7926737] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FBD12.
[2]	"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521 / FGSC 9021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U07801 Genomic DNA. Translation: AAA62242.1. AACP01000056 Genomic DNA. Translation: EAK82569.1.
RefSeq	XP_757661.1.
3D structure databases
SMR	Q99078. Positions 103-432.
ModBase	Search...
Protein-protein interaction databases
STRING	Q99078.
Genome annotation databases
GeneID	3629623.
KEGG	uma:UM01514.1.
Phylogenomic databases
eggNOG	fuNOG07450.
HOGENOM	HBG755340.
OMA	IRKKRNF.
OrthoDB	EOG9615MC.
PhylomeDB	Q99078.
Enzyme and pathway databases
BRENDA	2.7.12.2. 2320.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUZ7_USTMA

Accession

Primary (citable) accession number: Q99078
Secondary accession number(s): Q4PEE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 9, 2007
Last modified:	January 19, 2010
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)