Q99068 (AMRP_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-2-macroglobulin receptor-associated protein
Short name=Alpha-2-MRAP

Alternative name(s):
Gp330-binding 45 kDa protein
Low density lipoprotein receptor-related protein-associated protein 1
Short name=RAP

Gene names

Name:

Lrpap1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.
Subunit structure	Present on cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. Binds LRP1B; binding is followed by internalization and degradation By similarity.
Subcellular location	Endoplasmic reticulum By similarity. Cytoplasm By similarity. Cell surface By similarity. Note: Intracellular and associated with cell surface receptors. Found in the endoplasmic reticulum By similarity.
Sequence similarities	Belongs to the alpha-2-MRAP family.
Caution	Was originally (Ref.3) thought to be Heymann nephritis antigen gp330.

Ontologies

Keywords
Cellular component	Cytoplasm Endoplasmic reticulum
Domain	Coiled coil Signal
Ligand	Heparin-binding
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	extracellular negative regulation of signal transduction Inferred from electronic annotation. Source: Compara negative regulation of beta-amyloid clearance Inferred from electronic annotation. Source: Compara negative regulation of protein binding Inferred from electronic annotation. Source: Compara negative regulation of very-low-density lipoprotein particle clearance Inferred from electronic annotation. Source: Compara
Cellular_component	cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: Compara rough endoplasmic reticulum lumen Inferred from direct assay PubMed 8223699. Source: RGD vesicle Inferred from direct assay PubMed 8223699. Source: RGD
Molecular_function	heparin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Potential
	Chain	34 – 360	327	Alpha-2-macroglobulin receptor-associated protein		PRO_0000020726
Regions
	Region	240 – 356	117	LDL receptor binding Potential
	Coiled coil	222 – 302	81	Potential
	Motif	357 – 360	4	Prevents secretion from ER Potential
Amino acid modifications
	Glycosylation	271	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	38 – 40	3	YSR → RSA in AAA41269. Ref.3
	Sequence conflict	166 – 179	14	KFSSE…LWREF → ISVRLTSCARV Ref.3
	Sequence conflict	248 – 250	3	GYG → LR in AAA41269. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q99068 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: C7CF5ECCE1BCC5DC	FASTA	360	42,032
	10 20 30 40 50 60 MAPLRDRVST LPRLQLLVLL LLPLLLVPQP IAGHGGKYSR EKNEPEMAAK RESGEEFRME 70 80 90 100 110 120 KLNQLWEKAK RLHLSPVRLA ELHSDLKIQE RDELNWKKLK VEGLDGDGEK EAKLVHNLNV 130 140 150 160 170 180 ILARYGLDGR KDTQTVHSNA LNEDTQDELG DPRLEKLWHK AKTSGKFSSE ELDKLWREFL 190 200 210 220 230 240 HYKEKIHEYN VLLDTLSRAE EGYENLLSPS DMTHIKSDTL ASKHSELKDR LRSINQGLDR 250 260 270 280 290 300 LRKVSHQGYG PATEFEEPRV IDLWDLAQSA NFTEKELESF REELKHFEAK IEKHNHYQKQ 310 320 330 340 350 360 LEISHQKLKH VESIGDPEHI SRNKEKYVLL EEKTKELGYK VKKHLQDLSS RVSRARHNEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney and Placenta.
[2]	"Analysis of a 45-kDa protein that binds to the Heymann nephritis autoantigen GP330." Kanalas J.J., Makker S.P. J. Biol. Chem. 268:8188-8192(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-360. Strain: Sprague-Dawley. Tissue: Kidney.
[3]	"Molecular cloning of a cDNA encoding a major pathogenic domain of the Heymann nephritis antigen gp330." Pietromonaco S., Kerjaschki D., Binder S., Ullrich R., Farquhar M.G. Proc. Natl. Acad. Sci. U.S.A. 87:1811-1815(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 38-360.
[4]	"39-kDa protein modulates binding of ligands to low density lipoprotein receptor-related protein/alpha 2-macroglobulin receptor." Herz J., Goldstein J.L., Strickland D.K., Ho Y.K., Brown M.S. J. Biol. Chem. 266:21232-21238(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 38-51.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC082020 mRNA. Translation: AAH82020.1. BC098947 mRNA. Translation: AAH98947.1. Z11994 mRNA. Translation: CAA78040.1. Z11995 mRNA. Translation: CAA78041.1. M31051 mRNA. Translation: AAA41269.1.
IPI	IPI00364124.
PIR	A46646.
RefSeq	NP_001162584.1. NM_001169113.1.
UniGene	Rn.10293.
3D structure databases
ProteinModelPortal	Q99068.
SMR	Q99068. Positions 38-360.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q99068. 1 interaction.
MINT	MINT-4611866.
PTM databases
PhosphoSite	Q99068.
Proteomic databases
PaxDb	Q99068.
PRIDE	Q99068.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000012665; ENSRNOP00000012665; ENSRNOG00000009313.
GeneID	116565.
KEGG	rno:116565.
Organism-specific databases
CTD	4043.
RGD	620700. Lrpap1.
Phylogenomic databases
eggNOG	NOG323629.
GeneTree	ENSGT00390000004855.
HOGENOM	HOG000033926.
HOVERGEN	HBG000197.
InParanoid	Q99068.
OMA	FRMAKLN.
OrthoDB	EOG479F7K.
Gene expression databases
Genevestigator	Q99068.
GermOnline	ENSRNOG00000009313. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.81.10. 1 hit.
InterPro	IPR010483. Alpha_2_MRAP_C. IPR009066. MG_RAP_rcpt_1. [Graphical view]
Pfam	PF06401. Alpha-2-MRAP_C. 1 hit. PF06400. Alpha-2-MRAP_N. 1 hit. [Graphical view]
SUPFAM	SSF47045. MG_RAP_rcpt_1. 3 hits.
PROSITE	PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	619245.

Entry information

Entry name

AMRP_RAT

Accession

Primary (citable) accession number: Q99068
Secondary accession number(s): Q4FZX8, Q642A1, Q64723

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 6, 2005
Last modified:	April 3, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program