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Protein

Alpha-2-macroglobulin receptor-associated protein

Gene

Lrpap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway.By similarity

GO - Molecular functioni

  • heparin binding Source: UniProtKB-KW
  • lipase binding Source: RGD
  • low-density lipoprotein particle receptor binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2-macroglobulin receptor-associated protein
Short name:
Alpha-2-MRAP
Alternative name(s):
Gp330-binding 45 kDa protein
Low density lipoprotein receptor-related protein-associated protein 1
Short name:
RAP
Gene namesi
Name:Lrpap1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi620700. Lrpap1.

Subcellular locationi

  • Rough endoplasmic reticulum lumen By similarity
  • Endoplasmic reticulum-Golgi intermediate compartment lumen By similarity
  • Golgi apparatuscis-Golgi network By similarity
  • Golgi apparatus lumen By similarity
  • Endosome lumen By similarity
  • Cell surface By similarity

  • Note: May be associated with receptors at the cell surface.By similarity

GO - Cellular componenti

  • cell surface Source: UniProtKB-SubCell
  • cis-Golgi network Source: UniProtKB
  • cytoplasm Source: RGD
  • endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
  • endosome Source: UniProtKB
  • extracellular region Source: GOC
  • Golgi apparatus Source: UniProtKB
  • plasma membrane Source: Ensembl
  • rough endoplasmic reticulum Source: RGD
  • rough endoplasmic reticulum lumen Source: RGD
  • vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Endosome, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000002072634 – 360Alpha-2-macroglobulin receptor-associated proteinAdd BLAST327

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53PhosphoserineCombined sources1
Modified residuei138PhosphoserineBy similarity1
Glycosylationi271N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ99068.
PRIDEiQ99068.

PTM databases

iPTMnetiQ99068.
PhosphoSitePlusiQ99068.
SwissPalmiQ99068.

Expressioni

Gene expression databases

BgeeiENSRNOG00000009313.
GenevisibleiQ99068. RN.

Interactioni

Subunit structurei

Interacts with the LRP1/alpha-2-macroglobulin receptor heavy and light chains; the interaction is transient and coincides with a reduction of ligand binding by the receptor (PubMed:1400426). Interacts with LRP2/glycoprotein 330 (PubMed:1400426). Interacts with LRP1B; binding is followed by internalization and degradation. Interacts with LDLR (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Lrp8Q924X62EBI-919734,EBI-432319From a different organism.
VLDLRP981552EBI-919734,EBI-9004309From a different organism.

GO - Molecular functioni

  • lipase binding Source: RGD
  • low-density lipoprotein particle receptor binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ99068. 5 interactors.
MINTiMINT-4611866.
STRINGi10116.ENSRNOP00000012665.

Structurei

3D structure databases

ProteinModelPortaliQ99068.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni240 – 356LDL receptor bindingSequence analysisAdd BLAST117

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili222 – 302Sequence analysisAdd BLAST81

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi357 – 360Prevents secretion from ERBy similarity4

Sequence similaritiesi

Belongs to the alpha-2-MRAP family.Curated

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

eggNOGiKOG3956. Eukaryota.
ENOG410YJFP. LUCA.
GeneTreeiENSGT00390000004855.
HOGENOMiHOG000033926.
HOVERGENiHBG000197.
InParanoidiQ99068.
OMAiNQVWEKA.
OrthoDBiEOG091G0EO4.
PhylomeDBiQ99068.
TreeFamiTF320678.

Family and domain databases

Gene3Di1.20.81.10. 1 hit.
InterProiIPR010483. Alpha_2_MRAP_C.
IPR009066. MG_RAP_rcpt_1.
[Graphical view]
PfamiPF06401. Alpha-2-MRAP_C. 1 hit.
PF06400. Alpha-2-MRAP_N. 1 hit.
[Graphical view]
SUPFAMiSSF47045. SSF47045. 3 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99068-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPLRDRVST LPRLQLLVLL LLPLLLVPQP IAGHGGKYSR EKNEPEMAAK
60 70 80 90 100
RESGEEFRME KLNQLWEKAK RLHLSPVRLA ELHSDLKIQE RDELNWKKLK
110 120 130 140 150
VEGLDGDGEK EAKLVHNLNV ILARYGLDGR KDTQTVHSNA LNEDTQDELG
160 170 180 190 200
DPRLEKLWHK AKTSGKFSSE ELDKLWREFL HYKEKIHEYN VLLDTLSRAE
210 220 230 240 250
EGYENLLSPS DMTHIKSDTL ASKHSELKDR LRSINQGLDR LRKVSHQGYG
260 270 280 290 300
PATEFEEPRV IDLWDLAQSA NFTEKELESF REELKHFEAK IEKHNHYQKQ
310 320 330 340 350
LEISHQKLKH VESIGDPEHI SRNKEKYVLL EEKTKELGYK VKKHLQDLSS
360
RVSRARHNEL
Length:360
Mass (Da):42,032
Last modified:December 6, 2005 - v2
Checksum:iC7CF5ECCE1BCC5DC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38 – 40YSR → RSA in AAA41269 (PubMed:2408041).Curated3
Sequence conflicti166 – 179KFSSE…LWREF → ISVRLTSCARV (PubMed:2408041).CuratedAdd BLAST14
Sequence conflicti248 – 250GYG → LR in AAA41269 (PubMed:2408041).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082020 mRNA. Translation: AAH82020.1.
BC098947 mRNA. Translation: AAH98947.1.
Z11994 mRNA. Translation: CAA78040.1.
Z11995 mRNA. Translation: CAA78041.1.
M31051 mRNA. Translation: AAA41269.1.
PIRiA46646.
RefSeqiNP_001162584.1. NM_001169113.1.
UniGeneiRn.10293.

Genome annotation databases

EnsembliENSRNOT00000012665; ENSRNOP00000012665; ENSRNOG00000009313.
GeneIDi116565.
KEGGirno:116565.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082020 mRNA. Translation: AAH82020.1.
BC098947 mRNA. Translation: AAH98947.1.
Z11994 mRNA. Translation: CAA78040.1.
Z11995 mRNA. Translation: CAA78041.1.
M31051 mRNA. Translation: AAA41269.1.
PIRiA46646.
RefSeqiNP_001162584.1. NM_001169113.1.
UniGeneiRn.10293.

3D structure databases

ProteinModelPortaliQ99068.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99068. 5 interactors.
MINTiMINT-4611866.
STRINGi10116.ENSRNOP00000012665.

PTM databases

iPTMnetiQ99068.
PhosphoSitePlusiQ99068.
SwissPalmiQ99068.

Proteomic databases

PaxDbiQ99068.
PRIDEiQ99068.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012665; ENSRNOP00000012665; ENSRNOG00000009313.
GeneIDi116565.
KEGGirno:116565.

Organism-specific databases

CTDi4043.
RGDi620700. Lrpap1.

Phylogenomic databases

eggNOGiKOG3956. Eukaryota.
ENOG410YJFP. LUCA.
GeneTreeiENSGT00390000004855.
HOGENOMiHOG000033926.
HOVERGENiHBG000197.
InParanoidiQ99068.
OMAiNQVWEKA.
OrthoDBiEOG091G0EO4.
PhylomeDBiQ99068.
TreeFamiTF320678.

Miscellaneous databases

PROiQ99068.

Gene expression databases

BgeeiENSRNOG00000009313.
GenevisibleiQ99068. RN.

Family and domain databases

Gene3Di1.20.81.10. 1 hit.
InterProiIPR010483. Alpha_2_MRAP_C.
IPR009066. MG_RAP_rcpt_1.
[Graphical view]
PfamiPF06401. Alpha-2-MRAP_C. 1 hit.
PF06400. Alpha-2-MRAP_N. 1 hit.
[Graphical view]
SUPFAMiSSF47045. SSF47045. 3 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMRP_RAT
AccessioniPrimary (citable) accession number: Q99068
Secondary accession number(s): Q4FZX8, Q642A1, Q64723
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 6, 2005
Last modified: November 30, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be Heymann nephritis antigen gp330.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.