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Protein

Alpha-2-macroglobulin receptor-associated protein

Gene

Lrpap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.

GO - Molecular functioni

  1. heparin binding Source: UniProtKB-KW
  2. lipase binding Source: RGD

GO - Biological processi

  1. extracellular negative regulation of signal transduction Source: Ensembl
  2. negative regulation of beta-amyloid clearance Source: Ensembl
  3. negative regulation of protein binding Source: Ensembl
  4. negative regulation of very-low-density lipoprotein particle clearance Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2-macroglobulin receptor-associated protein
Short name:
Alpha-2-MRAP
Alternative name(s):
Gp330-binding 45 kDa protein
Low density lipoprotein receptor-related protein-associated protein 1
Short name:
RAP
Gene namesi
Name:Lrpap1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 14

Organism-specific databases

RGDi620700. Lrpap1.

Subcellular locationi

Endoplasmic reticulum PROSITE-ProRule annotation. Cytoplasm By similarity. Cell surface By similarity
Note: Intracellular and associated with cell surface receptors. Found in the endoplasmic reticulum (By similarity).By similarity

GO - Cellular componenti

  1. cell surface Source: UniProtKB-SubCell
  2. extracellular region Source: Ensembl
  3. plasma membrane Source: Ensembl
  4. rough endoplasmic reticulum lumen Source: RGD
  5. vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Sequence AnalysisAdd
BLAST
Chaini34 – 360327Alpha-2-macroglobulin receptor-associated proteinPRO_0000020726Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi271 – 2711N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ99068.
PRIDEiQ99068.

PTM databases

PhosphoSiteiQ99068.

Expressioni

Gene expression databases

GenevestigatoriQ99068.

Interactioni

Subunit structurei

Present on cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. Binds LRP1B; binding is followed by internalization and degradation (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Lrp8Q924X62EBI-919734,EBI-432319From a different organism.
VLDLRP981552EBI-919734,EBI-9004309From a different organism.

Protein-protein interaction databases

IntActiQ99068. 4 interactions.
MINTiMINT-4611866.

Structurei

3D structure databases

ProteinModelPortaliQ99068.
SMRiQ99068. Positions 38-360.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni240 – 356117LDL receptor bindingSequence AnalysisAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili222 – 30281Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi357 – 3604Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the alpha-2-MRAP family.Curated

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

eggNOGiNOG323629.
GeneTreeiENSGT00390000004855.
HOGENOMiHOG000033926.
HOVERGENiHBG000197.
InParanoidiQ99068.
OMAiNQVWEKA.
OrthoDBiEOG7P5T15.
PhylomeDBiQ99068.
TreeFamiTF320678.

Family and domain databases

Gene3Di1.20.81.10. 1 hit.
InterProiIPR010483. Alpha_2_MRAP_C.
IPR009066. MG_RAP_rcpt_1.
[Graphical view]
PfamiPF06401. Alpha-2-MRAP_C. 1 hit.
PF06400. Alpha-2-MRAP_N. 1 hit.
[Graphical view]
SUPFAMiSSF47045. SSF47045. 3 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99068-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPLRDRVST LPRLQLLVLL LLPLLLVPQP IAGHGGKYSR EKNEPEMAAK
60 70 80 90 100
RESGEEFRME KLNQLWEKAK RLHLSPVRLA ELHSDLKIQE RDELNWKKLK
110 120 130 140 150
VEGLDGDGEK EAKLVHNLNV ILARYGLDGR KDTQTVHSNA LNEDTQDELG
160 170 180 190 200
DPRLEKLWHK AKTSGKFSSE ELDKLWREFL HYKEKIHEYN VLLDTLSRAE
210 220 230 240 250
EGYENLLSPS DMTHIKSDTL ASKHSELKDR LRSINQGLDR LRKVSHQGYG
260 270 280 290 300
PATEFEEPRV IDLWDLAQSA NFTEKELESF REELKHFEAK IEKHNHYQKQ
310 320 330 340 350
LEISHQKLKH VESIGDPEHI SRNKEKYVLL EEKTKELGYK VKKHLQDLSS
360
RVSRARHNEL
Length:360
Mass (Da):42,032
Last modified:December 6, 2005 - v2
Checksum:iC7CF5ECCE1BCC5DC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 403YSR → RSA in AAA41269. (PubMed:2408041)Curated
Sequence conflicti166 – 17914KFSSE…LWREF → ISVRLTSCARV(PubMed:2408041)CuratedAdd
BLAST
Sequence conflicti248 – 2503GYG → LR in AAA41269. (PubMed:2408041)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082020 mRNA. Translation: AAH82020.1.
BC098947 mRNA. Translation: AAH98947.1.
Z11994 mRNA. Translation: CAA78040.1.
Z11995 mRNA. Translation: CAA78041.1.
M31051 mRNA. Translation: AAA41269.1.
PIRiA46646.
RefSeqiNP_001162584.1. NM_001169113.1.
UniGeneiRn.10293.

Genome annotation databases

EnsembliENSRNOT00000012665; ENSRNOP00000012665; ENSRNOG00000009313.
GeneIDi116565.
KEGGirno:116565.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082020 mRNA. Translation: AAH82020.1.
BC098947 mRNA. Translation: AAH98947.1.
Z11994 mRNA. Translation: CAA78040.1.
Z11995 mRNA. Translation: CAA78041.1.
M31051 mRNA. Translation: AAA41269.1.
PIRiA46646.
RefSeqiNP_001162584.1. NM_001169113.1.
UniGeneiRn.10293.

3D structure databases

ProteinModelPortaliQ99068.
SMRiQ99068. Positions 38-360.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ99068. 4 interactions.
MINTiMINT-4611866.

PTM databases

PhosphoSiteiQ99068.

Proteomic databases

PaxDbiQ99068.
PRIDEiQ99068.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012665; ENSRNOP00000012665; ENSRNOG00000009313.
GeneIDi116565.
KEGGirno:116565.

Organism-specific databases

CTDi4043.
RGDi620700. Lrpap1.

Phylogenomic databases

eggNOGiNOG323629.
GeneTreeiENSGT00390000004855.
HOGENOMiHOG000033926.
HOVERGENiHBG000197.
InParanoidiQ99068.
OMAiNQVWEKA.
OrthoDBiEOG7P5T15.
PhylomeDBiQ99068.
TreeFamiTF320678.

Miscellaneous databases

NextBioi619245.
PROiQ99068.

Gene expression databases

GenevestigatoriQ99068.

Family and domain databases

Gene3Di1.20.81.10. 1 hit.
InterProiIPR010483. Alpha_2_MRAP_C.
IPR009066. MG_RAP_rcpt_1.
[Graphical view]
PfamiPF06401. Alpha-2-MRAP_C. 1 hit.
PF06400. Alpha-2-MRAP_N. 1 hit.
[Graphical view]
SUPFAMiSSF47045. SSF47045. 3 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney and Placenta.
  2. "Analysis of a 45-kDa protein that binds to the Heymann nephritis autoantigen GP330."
    Kanalas J.J., Makker S.P.
    J. Biol. Chem. 268:8188-8192(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-360.
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  3. "Molecular cloning of a cDNA encoding a major pathogenic domain of the Heymann nephritis antigen gp330."
    Pietromonaco S., Kerjaschki D., Binder S., Ullrich R., Farquhar M.G.
    Proc. Natl. Acad. Sci. U.S.A. 87:1811-1815(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 38-360.
  4. "39-kDa protein modulates binding of ligands to low density lipoprotein receptor-related protein/alpha 2-macroglobulin receptor."
    Herz J., Goldstein J.L., Strickland D.K., Ho Y.K., Brown M.S.
    J. Biol. Chem. 266:21232-21238(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 38-51.

Entry informationi

Entry nameiAMRP_RAT
AccessioniPrimary (citable) accession number: Q99068
Secondary accession number(s): Q4FZX8, Q642A1, Q64723
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 6, 2005
Last modified: January 7, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be Heymann nephritis antigen gp330.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.