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Q99068

- AMRP_RAT

UniProt

Q99068 - AMRP_RAT

Protein

Alpha-2-macroglobulin receptor-associated protein

Gene

Lrpap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Interacts with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330.

    GO - Molecular functioni

    1. heparin binding Source: UniProtKB-KW
    2. lipase binding Source: RGD

    GO - Biological processi

    1. extracellular negative regulation of signal transduction Source: Ensembl
    2. negative regulation of beta-amyloid clearance Source: Ensembl
    3. negative regulation of protein binding Source: Ensembl
    4. negative regulation of very-low-density lipoprotein particle clearance Source: Ensembl

    Keywords - Ligandi

    Heparin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-2-macroglobulin receptor-associated protein
    Short name:
    Alpha-2-MRAP
    Alternative name(s):
    Gp330-binding 45 kDa protein
    Low density lipoprotein receptor-related protein-associated protein 1
    Short name:
    RAP
    Gene namesi
    Name:Lrpap1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 14

    Organism-specific databases

    RGDi620700. Lrpap1.

    Subcellular locationi

    Endoplasmic reticulum PROSITE-ProRule annotation. Cytoplasm By similarity. Cell surface By similarity
    Note: Intracellular and associated with cell surface receptors. Found in the endoplasmic reticulum By similarity.By similarity

    GO - Cellular componenti

    1. cell surface Source: UniProtKB-SubCell
    2. extracellular region Source: Ensembl
    3. plasma membrane Source: Ensembl
    4. rough endoplasmic reticulum lumen Source: RGD
    5. vesicle Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Sequence AnalysisAdd
    BLAST
    Chaini34 – 360327Alpha-2-macroglobulin receptor-associated proteinPRO_0000020726Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi271 – 2711N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ99068.
    PRIDEiQ99068.

    PTM databases

    PhosphoSiteiQ99068.

    Expressioni

    Gene expression databases

    GenevestigatoriQ99068.

    Interactioni

    Subunit structurei

    Present on cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. Binds LRP1B; binding is followed by internalization and degradation By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Lrp8Q924X62EBI-919734,EBI-432319From a different organism.
    VLDLRP981552EBI-919734,EBI-9004309From a different organism.

    Protein-protein interaction databases

    IntActiQ99068. 4 interactions.
    MINTiMINT-4611866.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99068.
    SMRiQ99068. Positions 38-360.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni240 – 356117LDL receptor bindingSequence AnalysisAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili222 – 30281Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi357 – 3604Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the alpha-2-MRAP family.Curated

    Keywords - Domaini

    Coiled coil, Signal

    Phylogenomic databases

    eggNOGiNOG323629.
    GeneTreeiENSGT00390000004855.
    HOGENOMiHOG000033926.
    HOVERGENiHBG000197.
    InParanoidiQ99068.
    OMAiFRMAKLN.
    OrthoDBiEOG7P5T15.
    PhylomeDBiQ99068.
    TreeFamiTF320678.

    Family and domain databases

    Gene3Di1.20.81.10. 1 hit.
    InterProiIPR010483. Alpha_2_MRAP_C.
    IPR009066. MG_RAP_rcpt_1.
    [Graphical view]
    PfamiPF06401. Alpha-2-MRAP_C. 1 hit.
    PF06400. Alpha-2-MRAP_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47045. SSF47045. 3 hits.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q99068-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPLRDRVST LPRLQLLVLL LLPLLLVPQP IAGHGGKYSR EKNEPEMAAK    50
    RESGEEFRME KLNQLWEKAK RLHLSPVRLA ELHSDLKIQE RDELNWKKLK 100
    VEGLDGDGEK EAKLVHNLNV ILARYGLDGR KDTQTVHSNA LNEDTQDELG 150
    DPRLEKLWHK AKTSGKFSSE ELDKLWREFL HYKEKIHEYN VLLDTLSRAE 200
    EGYENLLSPS DMTHIKSDTL ASKHSELKDR LRSINQGLDR LRKVSHQGYG 250
    PATEFEEPRV IDLWDLAQSA NFTEKELESF REELKHFEAK IEKHNHYQKQ 300
    LEISHQKLKH VESIGDPEHI SRNKEKYVLL EEKTKELGYK VKKHLQDLSS 350
    RVSRARHNEL 360
    Length:360
    Mass (Da):42,032
    Last modified:December 6, 2005 - v2
    Checksum:iC7CF5ECCE1BCC5DC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti38 – 403YSR → RSA in AAA41269. (PubMed:2408041)Curated
    Sequence conflicti166 – 17914KFSSE…LWREF → ISVRLTSCARV(PubMed:2408041)CuratedAdd
    BLAST
    Sequence conflicti248 – 2503GYG → LR in AAA41269. (PubMed:2408041)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC082020 mRNA. Translation: AAH82020.1.
    BC098947 mRNA. Translation: AAH98947.1.
    Z11994 mRNA. Translation: CAA78040.1.
    Z11995 mRNA. Translation: CAA78041.1.
    M31051 mRNA. Translation: AAA41269.1.
    PIRiA46646.
    RefSeqiNP_001162584.1. NM_001169113.1.
    UniGeneiRn.10293.

    Genome annotation databases

    EnsembliENSRNOT00000012665; ENSRNOP00000012665; ENSRNOG00000009313.
    GeneIDi116565.
    KEGGirno:116565.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC082020 mRNA. Translation: AAH82020.1 .
    BC098947 mRNA. Translation: AAH98947.1 .
    Z11994 mRNA. Translation: CAA78040.1 .
    Z11995 mRNA. Translation: CAA78041.1 .
    M31051 mRNA. Translation: AAA41269.1 .
    PIRi A46646.
    RefSeqi NP_001162584.1. NM_001169113.1.
    UniGenei Rn.10293.

    3D structure databases

    ProteinModelPortali Q99068.
    SMRi Q99068. Positions 38-360.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q99068. 4 interactions.
    MINTi MINT-4611866.

    PTM databases

    PhosphoSitei Q99068.

    Proteomic databases

    PaxDbi Q99068.
    PRIDEi Q99068.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000012665 ; ENSRNOP00000012665 ; ENSRNOG00000009313 .
    GeneIDi 116565.
    KEGGi rno:116565.

    Organism-specific databases

    CTDi 4043.
    RGDi 620700. Lrpap1.

    Phylogenomic databases

    eggNOGi NOG323629.
    GeneTreei ENSGT00390000004855.
    HOGENOMi HOG000033926.
    HOVERGENi HBG000197.
    InParanoidi Q99068.
    OMAi FRMAKLN.
    OrthoDBi EOG7P5T15.
    PhylomeDBi Q99068.
    TreeFami TF320678.

    Miscellaneous databases

    NextBioi 619245.
    PROi Q99068.

    Gene expression databases

    Genevestigatori Q99068.

    Family and domain databases

    Gene3Di 1.20.81.10. 1 hit.
    InterProi IPR010483. Alpha_2_MRAP_C.
    IPR009066. MG_RAP_rcpt_1.
    [Graphical view ]
    Pfami PF06401. Alpha-2-MRAP_C. 1 hit.
    PF06400. Alpha-2-MRAP_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47045. SSF47045. 3 hits.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney and Placenta.
    2. "Analysis of a 45-kDa protein that binds to the Heymann nephritis autoantigen GP330."
      Kanalas J.J., Makker S.P.
      J. Biol. Chem. 268:8188-8192(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-360.
      Strain: Sprague-Dawley.
      Tissue: Kidney.
    3. "Molecular cloning of a cDNA encoding a major pathogenic domain of the Heymann nephritis antigen gp330."
      Pietromonaco S., Kerjaschki D., Binder S., Ullrich R., Farquhar M.G.
      Proc. Natl. Acad. Sci. U.S.A. 87:1811-1815(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 38-360.
    4. "39-kDa protein modulates binding of ligands to low density lipoprotein receptor-related protein/alpha 2-macroglobulin receptor."
      Herz J., Goldstein J.L., Strickland D.K., Ho Y.K., Brown M.S.
      J. Biol. Chem. 266:21232-21238(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 38-51.

    Entry informationi

    Entry nameiAMRP_RAT
    AccessioniPrimary (citable) accession number: Q99068
    Secondary accession number(s): Q4FZX8, Q642A1, Q64723
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to be Heymann nephritis antigen gp330.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3