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Protein

Alpha-2-macroglobulin receptor-associated protein

Gene

Lrpap1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway.By similarity

Caution

Was originally thought to be Heymann nephritis antigen gp330.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHeparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2-macroglobulin receptor-associated protein
Short name:
Alpha-2-MRAP
Alternative name(s):
Gp330-binding 45 kDa protein
Low density lipoprotein receptor-related protein-associated protein 1
Short name:
RAP
Gene namesi
Name:Lrpap1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi620700 Lrpap1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Endosome, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000002072634 – 360Alpha-2-macroglobulin receptor-associated proteinAdd BLAST327

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53PhosphoserineCombined sources1
Modified residuei138PhosphoserineBy similarity1
Glycosylationi271N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ99068
PRIDEiQ99068

PTM databases

iPTMnetiQ99068
PhosphoSitePlusiQ99068
SwissPalmiQ99068

Expressioni

Gene expression databases

BgeeiENSRNOG00000009313
GenevisibleiQ99068 RN

Interactioni

Subunit structurei

Interacts with the LRP1/alpha-2-macroglobulin receptor heavy and light chains; the interaction is transient and coincides with a reduction of ligand binding by the receptor (PubMed:1400426). Interacts with LRP2/glycoprotein 330 (PubMed:1400426). Interacts with LRP1B; binding is followed by internalization and degradation. Interacts with LDLR (By similarity).By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi250500, 1 interactor
IntActiQ99068, 5 interactors
MINTiQ99068
STRINGi10116.ENSRNOP00000012665

Structurei

3D structure databases

ProteinModelPortaliQ99068
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni240 – 356LDL receptor bindingSequence analysisAdd BLAST117

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili222 – 302Sequence analysisAdd BLAST81

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi357 – 360Prevents secretion from ERBy similarity4

Sequence similaritiesi

Belongs to the alpha-2-MRAP family.Curated

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

eggNOGiKOG3956 Eukaryota
ENOG410YJFP LUCA
GeneTreeiENSGT00390000004855
HOGENOMiHOG000033926
HOVERGENiHBG000197
InParanoidiQ99068
KOiK22290
OMAiNQVWEKA
OrthoDBiEOG091G0EO4
PhylomeDBiQ99068
TreeFamiTF320678

Family and domain databases

CDDicd14806 RAP_D1, 1 hit
cd14807 RAP_D2, 1 hit
cd14808 RAP_D3, 1 hit
Gene3Di1.20.81.10, 3 hits
InterProiView protein in InterPro
IPR038003 A2-macroglobuin_RAP
IPR010483 Alpha_2_MRAP_C
IPR009066 MG_RAP_rcpt_1
IPR038001 RAP_D2
IPR037999 RAP_D3
IPR036744 RAP_sf
PANTHERiPTHR16560 PTHR16560, 1 hit
PfamiView protein in Pfam
PF06401 Alpha-2-MRAP_C, 1 hit
PF06400 Alpha-2-MRAP_N, 1 hit
SUPFAMiSSF47045 SSF47045, 3 hits
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99068-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPLRDRVST LPRLQLLVLL LLPLLLVPQP IAGHGGKYSR EKNEPEMAAK
60 70 80 90 100
RESGEEFRME KLNQLWEKAK RLHLSPVRLA ELHSDLKIQE RDELNWKKLK
110 120 130 140 150
VEGLDGDGEK EAKLVHNLNV ILARYGLDGR KDTQTVHSNA LNEDTQDELG
160 170 180 190 200
DPRLEKLWHK AKTSGKFSSE ELDKLWREFL HYKEKIHEYN VLLDTLSRAE
210 220 230 240 250
EGYENLLSPS DMTHIKSDTL ASKHSELKDR LRSINQGLDR LRKVSHQGYG
260 270 280 290 300
PATEFEEPRV IDLWDLAQSA NFTEKELESF REELKHFEAK IEKHNHYQKQ
310 320 330 340 350
LEISHQKLKH VESIGDPEHI SRNKEKYVLL EEKTKELGYK VKKHLQDLSS
360
RVSRARHNEL
Length:360
Mass (Da):42,032
Last modified:December 6, 2005 - v2
Checksum:iC7CF5ECCE1BCC5DC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38 – 40YSR → RSA in AAA41269 (PubMed:2408041).Curated3
Sequence conflicti166 – 179KFSSE…LWREF → ISVRLTSCARV (PubMed:2408041).CuratedAdd BLAST14
Sequence conflicti248 – 250GYG → LR in AAA41269 (PubMed:2408041).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC082020 mRNA Translation: AAH82020.1
BC098947 mRNA Translation: AAH98947.1
Z11994 mRNA Translation: CAA78040.1
Z11995 mRNA Translation: CAA78041.1
M31051 mRNA Translation: AAA41269.1
PIRiA46646
RefSeqiNP_001162584.1, NM_001169113.1
UniGeneiRn.10293

Genome annotation databases

EnsembliENSRNOT00000012665; ENSRNOP00000012665; ENSRNOG00000009313
GeneIDi116565
KEGGirno:116565

Similar proteinsi

Entry informationi

Entry nameiAMRP_RAT
AccessioniPrimary (citable) accession number: Q99068
Secondary accession number(s): Q4FZX8, Q642A1, Q64723
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 6, 2005
Last modified: May 23, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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