Laccase-2 precursor - Trametes villosa (White-rot fungus)

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Reviewed, UniProtKB/Swiss-Prot Q99046 (LAC2_TRAVI)

Last modified November 24, 2009. Version 55. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    Laccase-2
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 2
    Urishiol oxidase 2
    Diphenol oxidase 2

Gene names

Name:

LCC2

Organism

Trametes villosa (White-rot fungus)

Taxonomic identifier

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Aphyllophorales › Trametes

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products Probable.
Catalytic activity	4 benzenediol + O₂ = 4 benzosemiquinone + 2 H₂O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subunit structure	Homodimer.
Subcellular location	Secreted.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Secreted
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

20

Potential

Chain

499

Laccase-2

PRO_0000002944

Regions

Domain

126

Plastocyanin-like 1

Domain

143

Plastocyanin-like 2

Domain

123

Plastocyanin-like 3

Sites

Metal binding

1

Copper 1; type 2 By similarity

Metal binding

1

Copper 2; type 3 By similarity

Metal binding

1

Copper 2; type 3 By similarity

Metal binding

1

Copper 3; type 3 By similarity

Metal binding

1

Copper 4; type 1 By similarity

Metal binding

1

Copper 1; type 2 By similarity

Metal binding

1

Copper 3; type 3 By similarity

Metal binding

1

Copper 3; type 3 By similarity

Metal binding

1

Copper 4; type 1 By similarity

Metal binding

1

Copper 2; type 3 By similarity

Metal binding

1

Copper 4; type 1 By similarity

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q99046-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: DBB4EA526F684740
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519

55,777

        10         20         30         40         50         60 
MGLQRFSFFV TLALVARSLA AIGPVASLVV ANAPVSPDGF LRDAIVVNGV VPSPLITGKK 

        70         80         90        100        110        120 
GDRFQLNVVD TLTNHSMLKS TSIHWHGFFQ AGTNWAEGPA FVNQCPIASG HSFLYDFHVP 

       130        140        150        160        170        180 
DQAGTFWYHS HLSTQYCDGL RGPFVVYDPK DPHASRYDVD NESTVITLTD WYHTAARLGP 

       190        200        210        220        230        240 
KFPLGADATL INGLGRSAST PTAALAVINV QHGKRYRFRL VSISCDPNYT FSIDGHNLTV 

       250        260        270        280        290        300 
IEVDGINSQP LLVDSIQIFA AQRYSFVLNA NQTVGNYWVR ANPNFGTVGF AGGINSAILR 

       310        320        330        340        350        360 
YQGAPVAEPT TTQTPSVIPL IETNLHPLAR MPVPGSPTPG GVDKALNLAF NFNGTNFFIN 

       370        380        390        400        410        420 
NATFTPPTVP VLLQILSGAQ TAQDLLPAGS VYPLPAHSTI EITLPATALA PGAPHPFHLH 

       430        440        450        460        470        480 
GHAFAVVRSA GSTTYNYNDP IFRDVVSTGT PAAGDNVTIR FQTDNPGPWF LHCHIDFHLD 

       490        500        510 
AGFAIVFAED VADVKAANPV PKAWSDLCPI YDGLSEANQ

References

[1]

"Purification, characterization, molecular cloning, and expression of two laccase genes from the white rot basidiomycete Trametes villosa."
Yaver D.S., Xu F., Golightly E.J., Brown K.M., Brown S.H., Rey M.W., Schneider P., Halkier T., Mondorf K., Dalboge H.
Appl. Environ. Microbiol. 62:834-841(1996) [PubMed: 8975613] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Mycelium.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L49377 Genomic DNA. Translation: AAC41687.1.
3D structure databases
SMR	Q99046. Positions 21-519.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.10.3.2. 259651.
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 3 hits.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
PROSITE	PS00079. MULTICOPPER_OXIDASE1. 1 hit. PS00080. MULTICOPPER_OXIDASE2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC2_TRAVI

Accession

Primary (citable) accession number: Q99046

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents