Reviewed, UniProtKB/Swiss-Prot Q99041 (TGM4_RAT)

Names and origin

Protein names

Recommended name:
    Protein-glutamine gamma-glutamyltransferase 4
    EC=2.3.2.13
Alternative name(s):
    Transglutaminase-4
      Short name=TGase-4
    Dorsal prostate transglutaminase
    Dorsal protein 1
      Short name=DP1

Gene names

Name:	Tgm4
Synonyms:	Dp1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	668 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Associated with the mammalian reproductive process. Plays an important role in the formation of the seminal coagulum through the cross-linking of specific proteins present in the seminal plasma. Transglutaminase is also required to stabilize the copulatory plug.
Catalytic activity	Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subunit structure	Homodimer.
Subcellular location	Secreted.
Tissue specificity	Expressed in the coagulating gland and in the dorsal part of the prostate.
Induction	Androgen dependent, as shown by the decrease in the level of the protein following castration.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the transglutaminase superfamily. Transglutaminase family.

Ontologies

Keywords
Cellular component	Copulatory plug Secreted
Ligand	Calcium Metal-binding
Molecular function	Acyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	copulation Inferred from electronic annotation. Source: UniProtKB-KW peptide cross-linking Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein-glutamine gamma-glutamyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 668	668	Protein-glutamine gamma-glutamyltransferase 4		PRO_0000213712
Sites
	Active site	255	1	By similarity
	Active site	314	1	By similarity
	Active site	337	1	By similarity
	Metal binding	377	1	Calcium By similarity
	Metal binding	379	1	Calcium By similarity
	Metal binding	430	1	Calcium By similarity
	Metal binding	435	1	Calcium By similarity
Experimental info
	Sequence conflict	106	1	N → D in AAA41092. Ref.2
	Sequence conflict	279	1	F → FGVLTTALRAVGIPARSVTN F AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q99041-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6196DFAC7B63B294	FASTA	668	75,473
	10 20 30 40 50 60 MDSRNMLVVY SVNLEKKLNA AAHHTIEYQT QKLVLRRGQI FSLKVMLNRP LQSHDELKLI 70 80 90 100 110 120 FNTGHNMPFY TVELDPMTSY RSKGWQVKIA KQSGVEVVLN VISAANAVVG RYTMNVNEFD 130 140 150 160 170 180 AGVFFLLLNP WCSDDSVFMA SEEDRAEYVL NDTGYMYMGF AKQIKEKPWT FGRLRSTLEL 190 200 210 220 230 240 LLPIVDPFGA QGNAEPVLVS RAICTMMCAA NNFGVLVGNW TGDYSNGTAP YVWASSVPIL 250 260 270 280 290 300 QQHYITRMPV RFGQCWVFSG VLTTALRAVG IPARSVTNFE SAHDTEKNLR VDIYLDESGK 310 320 330 340 350 360 TIPHLTKDSV WNFHMWTDAW MKRQDLPQGH DGWQVLDSTP QEISEGQFRI GPSPVSAIRQ 370 380 390 400 410 420 GLVQFMYDTT FVFTEVNGDK YIWLVKQNQE RERRMSHRCG DCASIGKNIS TKMVGENRRQ 430 440 450 460 470 480 DITLHYKFPE GSPEERKAME KASGKRPDDK LNSRTLHISV LQNSVELGHP INLTIVLKRK 490 500 510 520 530 540 TATPQNVNIS CSLDLQTYTG NKKTNLGVIQ KTVQIQGQES EVSLSMDSSF YIYKLGMVDD 550 560 570 580 590 600 EMVIKGFIIA EIVDSGERVA TDTTLCFLYS AFSVEMPSTS KVNQPLTITC NFKNTLPIPL 610 620 630 640 650 660 TNIKFSVESL GLNNMKSWEQ ETVPPGKTIN FQIECTPVKT GNPRKFIVKF ISRQVKEVHA EKVVLITK

References

[1]	"Molecular cloning of rat prostate transglutaminase complementary DNA. The major androgen-regulated protein DP1 of rat dorsal prostate and coagulating gland." Ho K.-C., Quarmby V.E., French F.S., Wilson E.M. J. Biol. Chem. 267:12660-12667(1992) [PubMed: 1352290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Sprague-Dawley. Tissue: Prostate.
[2]	"Androgen regulated prostate genes: structural analysis and regulation." Ho K.-C., Wilson E.M., French F.S. Prog. Clin. Biol. Res. 239:125-153(1987) [PubMed: 3309953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 100-668, PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M90310 mRNA. Translation: AAA42287.1. M32725 mRNA. Translation: AAA41092.1.
IPI	IPI00197575.
PIR	A42803.
UniGene	Rn.9964
3D structure databases
HSSP	HSSP built from PDB template 1L9M based on UniProtKB Q08188.
ModBase	Search...
Proteomic databases
PRIDE	Q99041.
Genome annotation databases
Ensembl	ENSRNOG00000004320. Rattus norvegicus. [Contig view]
Organism-specific databases
RGD	620785. Tgm4.
Phylogenomic databases
HOVERGEN	Q99041.
Enzyme and pathway databases
BRENDA	2.3.2.13. 248.
Gene expression databases
ArrayExpress	Q99041.
GermOnline	ENSRNOG00000004320. Rattus norvegicus.
Family and domain databases
InterPro	IPR008957. Fibronectin_typ-III-like_fold. IPR013783. Ig-like_fold. IPR002931. Transglutaminase-like. IPR008958. Transglutaminase_C. IPR013808. Transglutaminase_CS. IPR001102. Transglutaminase_N. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00927. Transglut_C. 1 hit. PF01841. Transglut_core. 1 hit. PF00868. Transglut_N. 1 hit. [Graphical view]
SMART	SM00460. TGc. 1 hit. [Graphical view]
PROSITE	PS00547. TRANSGLUTAMINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TGM4_RAT

Accession

Primary (citable) accession number: Q99041

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)