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Protein

Protein-glutamine gamma-glutamyltransferase 4

Gene

Tgm4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Associated with the mammalian reproductive process. Plays an important role in the formation of the seminal coagulum through the cross-linking of specific proteins present in the seminal plasma. Transglutaminase is also required to stabilize the copulatory plug.2 Publications

Catalytic activityi

Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.PROSITE-ProRule annotation3 Publications

Cofactori

Ca2+1 PublicationNote: Binds 1 Ca2+ ion per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei256PROSITE-ProRule annotation1
Active sitei315PROSITE-ProRule annotation1
Active sitei338PROSITE-ProRule annotation1
Metal bindingi378CalciumBy similarity1
Metal bindingi380CalciumBy similarity1
Metal bindingi430CalciumBy similarity1
Metal bindingi435CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-glutamine gamma-glutamyltransferase 4 (EC:2.3.2.13)
Alternative name(s):
Dorsal prostate transglutaminase
Dorsal protein 1
Short name:
DP1
Transglutaminase-4
Short name:
TGase-4
Gene namesi
Name:Tgm4
Synonyms:Dp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620785. Tgm4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Copulatory plug, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002137121 – 667Protein-glutamine gamma-glutamyltransferase 4Add BLAST667

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi151N-linked (GlcNAc...)Sequence analysis1
Glycosylationi220N-linked (GlcNAc...)Sequence analysis1
Glycosylationi227N-linked (GlcNAc...)Sequence analysis1
Glycosylationi408N-linked (GlcNAc...)1 Publication1
Glycosylationi472N-linked (GlcNAc...)Sequence analysis1
Glycosylationi488N-linked (GlcNAc...)1 Publication1

Post-translational modificationi

The N-terminus is blocked.
Probably linked to the cell membrane via a lipid-anchor, possibly a GPI-anchor.
N-glycosylated on 2 Asn residues by a high mannose oligosaccharide consisting of five mannose residues and a fucosylated biantennary complex glycan.2 Publications

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ99041.
PRIDEiQ99041.

Expressioni

Tissue specificityi

Expressed in the coagulating gland, the dorsal part of the prostate and in semen (at protein level). Expressed at low levels in the lateral prostate and seminal vesicle. Not expressed in the epididymis, kidney, liver, serum, sperm plug, testes and ventral prostate.2 Publications

Inductioni

Androgen dependent, as shown by the decrease in the level of the protein following castration.2 Publications

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052677.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IFMV. Eukaryota.
ENOG410XQEZ. LUCA.
HOGENOMiHOG000231695.
HOVERGENiHBG004342.
InParanoidiQ99041.
KOiK05621.
PhylomeDBiQ99041.
TreeFamiTF324278.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
3.90.260.10. 1 hit.
InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002931. Transglutaminase-like.
IPR013808. Transglutaminase_AS.
IPR008958. Transglutaminase_C.
IPR001102. Transglutaminase_N.
[Graphical view]
PANTHERiPTHR11590. PTHR11590. 1 hit.
PfamiPF00927. Transglut_C. 1 hit.
PF01841. Transglut_core. 1 hit.
PF00868. Transglut_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
SMARTiSM00460. TGc. 1 hit.
[Graphical view]
SUPFAMiSSF49309. SSF49309. 2 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99041-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSRNMLVVY SVNLEKKLNA AAHHTIEYQT QKLVLRRGQI FSLKVMLNRP
60 70 80 90 100
LQSHDELKLI FNTGHNMPFY TVELDPMTSY RSKGWQVKIA KQSGVEVVLN
110 120 130 140 150
VISAADAVVG RYTMNVNEFD AGVFFLLFNP WCSDDSVFMA SEEDRAEYVL
160 170 180 190 200
NDTGYMYMGF AKQIKEKPWT FGQFEKYILN CCFRLLTHLE PKEMQSPVLV
210 220 230 240 250
SRAICTMMCA ANNFGVLVGN WTGDYSNGTA PYVWASSVPI LQQHYITRMP
260 270 280 290 300
VRFGQCWVFS GVLTTALRAV GIPARSVTNF ESAHDTEKNL RVDIYLDESG
310 320 330 340 350
KTIPHLTKDS VWNFHMWTDA WMKRQDLPQG HDGWQVLDST PQEISEGQFR
360 370 380 390 400
IGPSPVSAIR QGLVQIMYDT TFVFTEVNGD KYIWLVKQNQ EREKNVLIAV
410 420 430 440 450
ETASIGKNIS TKMVGENRRQ DITLHYKFPE GSPEERKAME KASGKRPDDK
460 470 480 490 500
LNSRTLHISV LQNSVELGHP INLTIVLKRK TATPQNVNIS CSLDLQTYTG
510 520 530 540 550
NKKTNLGVIQ KTVQIQGQES EVSLSMDSSF YIYKLGMVDD EMVIKGFIIA
560 570 580 590 600
EIVDSGERVA TDTTLCFLYS AFSVEMPSTS KVNQPLTITC NFKNTLPIPL
610 620 630 640 650
TNIKFSVESL GLNNMKSWEQ ETVPPGKTIN FQIECTPVKT GPRKFIVKFI
660
SRQVKEVHAE KVVLITK
Length:667
Mass (Da):75,587
Last modified:September 22, 2009 - v2
Checksum:iFE9AED50E4677E59
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106D → N in AAA42287 (PubMed:1352290).Curated1
Sequence conflicti128F → L in AAA42287 (PubMed:1352290).Curated1
Sequence conflicti128F → L in AAA41092 (PubMed:3309953).Curated1
Sequence conflicti173 – 196QFEKY…KEMQS → RLRSTLELLLPIVDPFGAQG NAE in AAA42287 (PubMed:1352290).CuratedAdd BLAST24
Sequence conflicti173 – 196QFEKY…KEMQS → RLRSTLELLLPIVDPFGAQG NAE in AAA41092 (PubMed:3309953).CuratedAdd BLAST24
Sequence conflicti280F → FGVLTTALRAVGIPARSVTN F in AAA41092 (PubMed:3309953).Curated1
Sequence conflicti280F → FGVLTTALRAVGIPARSVTN F AA sequence (PubMed:3309953).Curated1
Sequence conflicti316M → V in AAH66665 (PubMed:15489334).Curated1
Sequence conflicti366I → F in AAA42287 (PubMed:1352290).Curated1
Sequence conflicti366I → F in AAA41092 (PubMed:3309953).Curated1
Sequence conflicti394 – 402KNVLIAVET → RRMSHRCGDC in AAA42287 (PubMed:1352290).Curated9
Sequence conflicti394 – 402KNVLIAVET → RRMSHRCGDC in AAA41092 (PubMed:3309953).Curated9
Sequence conflicti641G → GN in AAA42287 (PubMed:1352290).Curated1
Sequence conflicti641G → GN in AAA41092 (PubMed:3309953).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90310 mRNA. Translation: AAA42287.1.
BC066665 mRNA. Translation: AAH66665.1.
M32725 mRNA. Translation: AAA41092.1.
PIRiA42803.
RefSeqiNP_073204.2. NM_022713.2.
UniGeneiRn.9964.

Genome annotation databases

GeneIDi64679.
KEGGirno:64679.
UCSCiRGD:620785. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90310 mRNA. Translation: AAA42287.1.
BC066665 mRNA. Translation: AAH66665.1.
M32725 mRNA. Translation: AAA41092.1.
PIRiA42803.
RefSeqiNP_073204.2. NM_022713.2.
UniGeneiRn.9964.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052677.

Proteomic databases

PaxDbiQ99041.
PRIDEiQ99041.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi64679.
KEGGirno:64679.
UCSCiRGD:620785. rat.

Organism-specific databases

CTDi7047.
RGDi620785. Tgm4.

Phylogenomic databases

eggNOGiENOG410IFMV. Eukaryota.
ENOG410XQEZ. LUCA.
HOGENOMiHOG000231695.
HOVERGENiHBG004342.
InParanoidiQ99041.
KOiK05621.
PhylomeDBiQ99041.
TreeFamiTF324278.

Miscellaneous databases

PROiQ99041.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
3.90.260.10. 1 hit.
InterProiIPR023608. Gln_gamma-glutamylTfrase_euk.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002931. Transglutaminase-like.
IPR013808. Transglutaminase_AS.
IPR008958. Transglutaminase_C.
IPR001102. Transglutaminase_N.
[Graphical view]
PANTHERiPTHR11590. PTHR11590. 1 hit.
PfamiPF00927. Transglut_C. 1 hit.
PF01841. Transglut_core. 1 hit.
PF00868. Transglut_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000459. TGM_EBP42. 1 hit.
SMARTiSM00460. TGc. 1 hit.
[Graphical view]
SUPFAMiSSF49309. SSF49309. 2 hits.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00547. TRANSGLUTAMINASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTGM4_RAT
AccessioniPrimary (citable) accession number: Q99041
Secondary accession number(s): Q6NYB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 22, 2009
Last modified: June 8, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.