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Protein

Catechol O-methyltransferase

Gene

COMT

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.

Catalytic activityi

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei7S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei29S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei37S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei55S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei56S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei84S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
Metal bindingi106MagnesiumBy similarity1
Binding sitei106S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei109SubstrateBy similarity1
Metal bindingi134MagnesiumBy similarity1
Metal bindingi135MagnesiumBy similarity1
Binding sitei135SubstrateBy similarity1
Binding sitei164SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Catecholamine metabolism, Neurotransmitter degradation

Keywords - Ligandi

Magnesium, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Catechol O-methyltransferase (EC:2.1.1.6)
Gene namesi
Name:COMT
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176837.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000090016‹1 – 186Catechol O-methyltransferaseAdd BLAST›186

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei182PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ99028.
PeptideAtlasiQ99028.
PRIDEiQ99028.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000010805.

Chemistry databases

BindingDBiQ99028.

Structurei

3D structure databases

ProteinModelPortaliQ99028.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 85S-adenosyl-L-methionine bindingPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1663. Eukaryota.
COG4122. LUCA.
HOGENOMiHOG000046392.
HOVERGENiHBG005376.
InParanoidiQ99028.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR017128. Catechol_O-MeTrfase_euk.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01596. Methyltransf_3. 1 hit.
[Graphical view]
PIRSFiPIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51682. SAM_OMT_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q99028-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
KERAMHVGRK KGQIVDTVVQ EQRPSVLLEL GAYCGYSAVR MARLLLPSAR
60 70 80 90 100
LLTIELNPDN AAIAQQVVDF AGLQDRVTVV VGASQDIIPQ LKKKYDVDTL
110 120 130 140 150
DMVFLDHWKD RYLPDTLLLE ECGLLRKGTV LLADNVICPG APDFLAHVRG
160 170 180
CGRFECTHFS SYLEYSQMVD GLEKAVYKGP GSPAQP
Length:186
Mass (Da):20,587
Last modified:June 1, 1994 - v1
Checksum:i493B8801F06C6262
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

UniGeneiSsc.46647.
Ssc.53711.

Cross-referencesi

Sequence databases

UniGeneiSsc.46647.
Ssc.53711.

3D structure databases

ProteinModelPortaliQ99028.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000010805.

Chemistry databases

BindingDBiQ99028.
ChEMBLiCHEMBL2176837.

Proteomic databases

PaxDbiQ99028.
PeptideAtlasiQ99028.
PRIDEiQ99028.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG1663. Eukaryota.
COG4122. LUCA.
HOGENOMiHOG000046392.
HOVERGENiHBG005376.
InParanoidiQ99028.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR017128. Catechol_O-MeTrfase_euk.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01596. Methyltransf_3. 1 hit.
[Graphical view]
PIRSFiPIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51682. SAM_OMT_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOMT_PIG
AccessioniPrimary (citable) accession number: Q99028
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 5, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.