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Q99028

- COMT_PIG

UniProt

Q99028 - COMT_PIG

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Protein

Catechol O-methyltransferase

Gene

COMT

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.

Catalytic activityi

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
Binding sitei29 – 291S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei37 – 371S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei55 – 551S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei56 – 561S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
Binding sitei84 – 841S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
Metal bindingi106 – 1061MagnesiumBy similarity
Binding sitei106 – 1061S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei109 – 1091SubstrateBy similarity
Metal bindingi134 – 1341MagnesiumBy similarity
Metal bindingi135 – 1351MagnesiumBy similarity
Binding sitei135 – 1351SubstrateBy similarity
Binding sitei164 – 1641SubstrateBy similarity

GO - Molecular functioni

  1. catechol O-methyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. catecholamine metabolic process Source: UniProtKB-KW
  2. neurotransmitter catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Catecholamine metabolism, Neurotransmitter degradation

Keywords - Ligandi

Magnesium, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Catechol O-methyltransferase (EC:2.1.1.6)
Gene namesi
Name:COMT
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity; Single-pass type II membrane protein By similarity; Extracellular side By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 186›186Catechol O-methyltransferasePRO_0000090016Add
BLAST

Proteomic databases

PaxDbiQ99028.
PRIDEiQ99028.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000010805.

Structurei

3D structure databases

ProteinModelPortaliQ99028.
SMRiQ99028. Positions 1-180.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 854S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG4122.
HOGENOMiHOG000046392.
HOVERGENiHBG005376.
InParanoidiQ99028.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025782. Catechol_O-MeTrfase.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10509. PTHR10509. 1 hit.
PfamiPF01596. Methyltransf_3. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51682. SAM_OMT_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q99028-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
KERAMHVGRK KGQIVDTVVQ EQRPSVLLEL GAYCGYSAVR MARLLLPSAR
60 70 80 90 100
LLTIELNPDN AAIAQQVVDF AGLQDRVTVV VGASQDIIPQ LKKKYDVDTL
110 120 130 140 150
DMVFLDHWKD RYLPDTLLLE ECGLLRKGTV LLADNVICPG APDFLAHVRG
160 170 180
CGRFECTHFS SYLEYSQMVD GLEKAVYKGP GSPAQP
Length:186
Mass (Da):20,587
Last modified:June 1, 1994 - v1
Checksum:i493B8801F06C6262
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Cross-referencesi

3D structure databases

ProteinModelPortali Q99028.
SMRi Q99028. Positions 1-180.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000010805.

Chemistry

BindingDBi Q99028.
ChEMBLi CHEMBL2176837.

Proteomic databases

PaxDbi Q99028.
PRIDEi Q99028.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG4122.
HOGENOMi HOG000046392.
HOVERGENi HBG005376.
InParanoidi Q99028.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR025782. Catechol_O-MeTrfase.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10509. PTHR10509. 1 hit.
Pfami PF01596. Methyltransf_3. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS51682. SAM_OMT_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Human catechol-O-methyltransferase: cloning and expression of the membrane-associated form."
    Bertocci B., Miggiano V., da Prada M., Dembic Z., Lahm H.-W., Malherbe P.
    Proc. Natl. Acad. Sci. U.S.A. 88:1416-1420(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Immunoaffinity purification and partial amino acid sequence analysis of catechol-O-methyltransferase from pig liver."
    Bertocci B., Garotta G., da Prada M., Lahm H.-W., Zurcher G., Virgallita G., Miggiano V.
    Biochim. Biophys. Acta 1080:103-109(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.

Entry informationi

Entry nameiCOMT_PIG
AccessioniPrimary (citable) accession number: Q99028
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 26, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3