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Q99027 (COMP_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sensor histidine kinase ComP

EC=2.7.13.3
Gene names
Name:comP
Ordered Locus Names:BSU31690
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length769 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Sensor in the two-component regulatory system ComP/ComA involved in a major quorum response pathway that regulates the development of genetic competence. Plays a role in sporulation, at least partly interchangeable with that of SpoIIJ. Probably activates ComA by phosphorylation. Ref.7

Catalytic activity

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Post-translational modification

Autophosphorylates on a histidine and transfers the phosphate group onto an aspartate in ComA, thus activating it.

Miscellaneous

The DNA sequences encoding comQ, comX and the N-terminal two-thirds of comP show a striking polymorphism, which determines the specificity of the quorum-sensing system in the different pherotypes of Bacillus.

Sequence similarities

Contains 1 histidine kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 769769Sensor histidine kinase ComP
PRO_0000074736

Regions

Topological domain1 – 99Cytoplasmic Potential
Transmembrane10 – 3324Helical; Potential
Topological domain34 – 11380Extracellular Potential
Transmembrane114 – 13421Helical; Potential
Topological domain135 – 14410Cytoplasmic Potential
Transmembrane145 – 16723Helical; Potential
Topological domain168 – 23568Extracellular Potential
Transmembrane236 – 25722Helical; Potential
Topological domain258 – 27215Cytoplasmic Potential
Transmembrane273 – 29523Helical; Potential
Topological domain296 – 2994Extracellular Potential
Transmembrane300 – 32324Helical; Potential
Topological domain324 – 33714Cytoplasmic Potential
Transmembrane338 – 35720Helical; Potential
Topological domain358 – 3614Extracellular Potential
Transmembrane362 – 38322Helical; Potential
Topological domain384 – 769386Cytoplasmic Potential
Domain571 – 769199Histidine kinase

Amino acid modifications

Modified residue5761Phosphohistidine; by autocatalysis By similarity

Experimental info

Sequence conflict641G → A in CAA37957. Ref.1
Sequence conflict641G → A in AAA22319. Ref.1
Sequence conflict641G → A in CAB07903. Ref.2
Sequence conflict6041C → S in CAB07903. Ref.2
Sequence conflict6101D → Y in CAA37957. Ref.1
Sequence conflict6101D → Y in AAA22319. Ref.1
Sequence conflict6281E → G in CAA37957. Ref.1
Sequence conflict6281E → G in AAA22319. Ref.1
Sequence conflict636 – 6372QL → PV in CAA37957. Ref.1
Sequence conflict636 – 6372QL → PV in AAA22319. Ref.1
Sequence conflict653 – 6542QQ → HE in CAA37957. Ref.1
Sequence conflict653 – 6542QQ → HE in AAA22319. Ref.1
Sequence conflict653 – 6542QQ → HE in CAB07903. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q99027 [UniParc].

Last modified July 7, 2009. Version 3.
Checksum: AF2DF976EED0EF40

FASTA76989,309
        10         20         30         40         50         60 
MKNLIKKFTI AVIVLSILYI SYTTYISMNG IIIGTKIHKN DKSQFMIEEI SESSYGQFVG 

        70         80         90        100        110        120 
LRQGDIILKI NKEKPSDKHL KWGYLSHINS LDILRSGKKI HLKDFDLVTL NRPYSFFLFV 

       130        140        150        160        170        180 
LPLFFYFLSI ICIFYILKVN KKRRSFAAYI LILLLLDISI AYISAGGPFR GHIINRYINL 

       190        200        210        220        230        240 
FTFISSPILY LQFIQRYLGE IGKTFLNRIS FLYIIPIFNL GIEFFQDYLQ VDIDFLATLN 

       250        260        270        280        290        300 
LVSFATLTLF SFSAIYLHLN KYKYAEHSFI LKLLILTNTL SFAPFLIFFV LPIIFTGNYI 

       310        320        330        340        350        360 
FPALASASLL VLIPFGLVYQ FVANKMFDIE FILGRMRYYA LLAMIPTLLI VGALVLFDVM 

       370        380        390        400        410        420 
DIQMNPVRQT VFFFVVMFAV FYFKEVMDFK FRLKRFSEKF NYQDSIFKYT QLMRGVTSLQ 

       430        440        450        460        470        480 
QVFKELKNTI LDVLLVSKAY TFEVTPDHKV IFLDKHEVGP DWNFYQEEFE NVTSEIGKII 

       490        500        510        520        530        540 
EVNQGFLMKV GERGGSSYVL LCLSNINTPR LTRDEISWLK TLSFYTSVSM ENVLHIEELM 

       550        560        570        580        590        600 
EHLKDLKQEG TNPIWLKKLM FAIEEKQRSG LARDLHDSVL QDLISLKRQC ELFLGDFKKD 

       610        620        630        640        650        660 
DNPCREEVQD KLVQMNEQMS DVISMTRETC HELRPQLLYD LGLVKALSKL VAQQQERVPF 

       670        680        690        700        710        720 
HIRLNTGRFT ASLDLDSQLN LYRIIQEFLS NAVKHSQATD VLIMLISIQN KIVLHYEDDG 

       730        740        750        760 
VGFDQEKNTE HSMSMGLSGI KERVRALDGR LRIETSEGKG FKADIEIEL 

« Hide

References

« Hide 'large scale' references
[1]"A Bacillus subtilis regulatory gene product for genetic competence and sporulation resembles sensor protein members of the bacterial two-component signal-transduction systems."
Weinrauch Y., Penchev R., Dubnau E., Smith I., Dubnau D.
Genes Dev. 4:860-872(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BD630.
[2]"Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai)."
Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H., Duesterhoeft A., Pohl T.M., Weitzenegger T.
Microbiology 143:2769-2774(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 64; 604 AND 654.
[5]"Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis."
Weinrauch Y., Msadek T., Kunst F., Dubnau D.
J. Bacteriol. 173:5685-5693(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16.
Strain: 168.
[6]"Specificity and genetic polymorphism of the Bacillus competence quorum-sensing system."
Tortosa P., Logsdon L., Kraigher B., Itoh Y., Mandic-Mulec I., Dubnau D.
J. Bacteriol. 183:451-460(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: POLYMORPHISM IN COMX; COMQ AND COMP.
[7]"Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis."
Comella N., Grossman A.D.
Mol. Microbiol. 57:1159-1174(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54010 mRNA. Translation: CAA37957.1.
M22856 Genomic DNA. Translation: AAA22319.1.
Z93932 Genomic DNA. Translation: CAB07903.1.
AL009126 Genomic DNA. Translation: CAB15157.2.
M71283 Genomic DNA. Translation: AAA22324.1.
PIRA35848.
B69604.
RefSeqNP_391047.2. NC_000964.3.

3D structure databases

ProteinModelPortalQ99027.
SMRQ99027. Positions 31-76, 563-769.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU31690.

Proteomic databases

PaxDbQ99027.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15157; CAB15157; BSU31690.
GeneID938866.
KEGGbsu:BSU31690.
PATRIC18978308. VBIBacSub10457_3317.

Organism-specific databases

GenoListBSU31690. [Micado]

Phylogenomic databases

eggNOGCOG4585.
HOGENOMHOG000246978.
KOK07680.
OrthoDBEOG6W9X4M.

Enzyme and pathway databases

BioCycBSUB:BSU31690-MONOMER.

Family and domain databases

Gene3D3.30.565.10. 2 hits.
InterProIPR003594. HATPase_ATP-bd.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
IPR005467. Sig_transdc_His_kinase_core.
[Graphical view]
PfamPF02518. HATPase_c. 1 hit.
PF07730. HisKA_3. 1 hit.
[Graphical view]
SMARTSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMSSF55874. SSF55874. 1 hit.
PROSITEPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCOMP_BACSU
AccessionPrimary (citable) accession number: Q99027
Secondary accession number(s): O05226
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 7, 2009
Last modified: July 9, 2014
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList