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Q99027

- COMP_BACSU

UniProt

Q99027 - COMP_BACSU

Protein

Sensor histidine kinase ComP

Gene

comP

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 3 (07 Jul 2009)
      Previous versions | rss
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    Functioni

    Sensor in the two-component regulatory system ComP/ComA involved in a major quorum response pathway that regulates the development of genetic competence. Plays a role in sporulation, at least partly interchangeable with that of SpoIIJ. Probably activates ComA by phosphorylation.1 Publication

    Catalytic activityi

    ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. phosphorelay sensor kinase activity Source: InterPro

    GO - Biological processi

    1. establishment of competence for transformation Source: UniProtKB-KW
    2. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Competence, Sporulation, Two-component regulatory system

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU31690-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sensor histidine kinase ComP (EC:2.7.13.3)
    Gene namesi
    Name:comP
    Ordered Locus Names:BSU31690
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU31690. [Micado]

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 769769Sensor histidine kinase ComPPRO_0000074736Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei576 – 5761Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

    Post-translational modificationi

    Autophosphorylates on a histidine and transfers the phosphate group onto an aspartate in ComA, thus activating it.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ99027.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU31690.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99027.
    SMRiQ99027. Positions 31-76, 563-769.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 99CytoplasmicSequence Analysis
    Topological domaini34 – 11380ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini135 – 14410CytoplasmicSequence Analysis
    Topological domaini168 – 23568ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini258 – 27215CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini296 – 2994ExtracellularSequence Analysis
    Topological domaini324 – 33714CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini358 – 3614ExtracellularSequence Analysis
    Topological domaini384 – 769386CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei10 – 3324HelicalSequence AnalysisAdd
    BLAST
    Transmembranei114 – 13421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei145 – 16723HelicalSequence AnalysisAdd
    BLAST
    Transmembranei236 – 25722HelicalSequence AnalysisAdd
    BLAST
    Transmembranei273 – 29523HelicalSequence AnalysisAdd
    BLAST
    Transmembranei300 – 32324HelicalSequence AnalysisAdd
    BLAST
    Transmembranei338 – 35720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei362 – 38322HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini571 – 769199Histidine kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 histidine kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG4585.
    HOGENOMiHOG000246978.
    KOiK07680.
    OrthoDBiEOG6W9X4M.

    Family and domain databases

    Gene3Di3.30.565.10. 2 hits.
    InterProiIPR003594. HATPase_ATP-bd.
    IPR011712. Sig_transdc_His_kin_sub3_dim/P.
    IPR005467. Sig_transdc_His_kinase_core.
    [Graphical view]
    PfamiPF02518. HATPase_c. 1 hit.
    PF07730. HisKA_3. 1 hit.
    [Graphical view]
    SMARTiSM00387. HATPase_c. 1 hit.
    [Graphical view]
    SUPFAMiSSF55874. SSF55874. 1 hit.
    PROSITEiPS50109. HIS_KIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99027-1 [UniParc]FASTAAdd to Basket

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    MKNLIKKFTI AVIVLSILYI SYTTYISMNG IIIGTKIHKN DKSQFMIEEI    50
    SESSYGQFVG LRQGDIILKI NKEKPSDKHL KWGYLSHINS LDILRSGKKI 100
    HLKDFDLVTL NRPYSFFLFV LPLFFYFLSI ICIFYILKVN KKRRSFAAYI 150
    LILLLLDISI AYISAGGPFR GHIINRYINL FTFISSPILY LQFIQRYLGE 200
    IGKTFLNRIS FLYIIPIFNL GIEFFQDYLQ VDIDFLATLN LVSFATLTLF 250
    SFSAIYLHLN KYKYAEHSFI LKLLILTNTL SFAPFLIFFV LPIIFTGNYI 300
    FPALASASLL VLIPFGLVYQ FVANKMFDIE FILGRMRYYA LLAMIPTLLI 350
    VGALVLFDVM DIQMNPVRQT VFFFVVMFAV FYFKEVMDFK FRLKRFSEKF 400
    NYQDSIFKYT QLMRGVTSLQ QVFKELKNTI LDVLLVSKAY TFEVTPDHKV 450
    IFLDKHEVGP DWNFYQEEFE NVTSEIGKII EVNQGFLMKV GERGGSSYVL 500
    LCLSNINTPR LTRDEISWLK TLSFYTSVSM ENVLHIEELM EHLKDLKQEG 550
    TNPIWLKKLM FAIEEKQRSG LARDLHDSVL QDLISLKRQC ELFLGDFKKD 600
    DNPCREEVQD KLVQMNEQMS DVISMTRETC HELRPQLLYD LGLVKALSKL 650
    VAQQQERVPF HIRLNTGRFT ASLDLDSQLN LYRIIQEFLS NAVKHSQATD 700
    VLIMLISIQN KIVLHYEDDG VGFDQEKNTE HSMSMGLSGI KERVRALDGR 750
    LRIETSEGKG FKADIEIEL 769
    Length:769
    Mass (Da):89,309
    Last modified:July 7, 2009 - v3
    Checksum:iAF2DF976EED0EF40
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti64 – 641G → A in CAA37957. (PubMed:2116363)Curated
    Sequence conflicti64 – 641G → A in AAA22319. (PubMed:2116363)Curated
    Sequence conflicti64 – 641G → A in CAB07903. (PubMed:9274030)Curated
    Sequence conflicti604 – 6041C → S in CAB07903. (PubMed:9274030)Curated
    Sequence conflicti610 – 6101D → Y in CAA37957. (PubMed:2116363)Curated
    Sequence conflicti610 – 6101D → Y in AAA22319. (PubMed:2116363)Curated
    Sequence conflicti628 – 6281E → G in CAA37957. (PubMed:2116363)Curated
    Sequence conflicti628 – 6281E → G in AAA22319. (PubMed:2116363)Curated
    Sequence conflicti636 – 6372QL → PV in CAA37957. (PubMed:2116363)Curated
    Sequence conflicti636 – 6372QL → PV in AAA22319. (PubMed:2116363)Curated
    Sequence conflicti653 – 6542QQ → HE in CAA37957. (PubMed:2116363)Curated
    Sequence conflicti653 – 6542QQ → HE in AAA22319. (PubMed:2116363)Curated
    Sequence conflicti653 – 6542QQ → HE in CAB07903. (PubMed:9274030)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54010 mRNA. Translation: CAA37957.1.
    M22856 Genomic DNA. Translation: AAA22319.1.
    Z93932 Genomic DNA. Translation: CAB07903.1.
    AL009126 Genomic DNA. Translation: CAB15157.2.
    M71283 Genomic DNA. Translation: AAA22324.1.
    PIRiA35848.
    B69604.
    RefSeqiNP_391047.2. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB15157; CAB15157; BSU31690.
    GeneIDi938866.
    KEGGibsu:BSU31690.
    PATRICi18978308. VBIBacSub10457_3317.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54010 mRNA. Translation: CAA37957.1 .
    M22856 Genomic DNA. Translation: AAA22319.1 .
    Z93932 Genomic DNA. Translation: CAB07903.1 .
    AL009126 Genomic DNA. Translation: CAB15157.2 .
    M71283 Genomic DNA. Translation: AAA22324.1 .
    PIRi A35848.
    B69604.
    RefSeqi NP_391047.2. NC_000964.3.

    3D structure databases

    ProteinModelPortali Q99027.
    SMRi Q99027. Positions 31-76, 563-769.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU31690.

    Proteomic databases

    PaxDbi Q99027.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB15157 ; CAB15157 ; BSU31690 .
    GeneIDi 938866.
    KEGGi bsu:BSU31690.
    PATRICi 18978308. VBIBacSub10457_3317.

    Organism-specific databases

    GenoListi BSU31690. [Micado ]

    Phylogenomic databases

    eggNOGi COG4585.
    HOGENOMi HOG000246978.
    KOi K07680.
    OrthoDBi EOG6W9X4M.

    Enzyme and pathway databases

    BioCyci BSUB:BSU31690-MONOMER.

    Family and domain databases

    Gene3Di 3.30.565.10. 2 hits.
    InterProi IPR003594. HATPase_ATP-bd.
    IPR011712. Sig_transdc_His_kin_sub3_dim/P.
    IPR005467. Sig_transdc_His_kinase_core.
    [Graphical view ]
    Pfami PF02518. HATPase_c. 1 hit.
    PF07730. HisKA_3. 1 hit.
    [Graphical view ]
    SMARTi SM00387. HATPase_c. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55874. SSF55874. 1 hit.
    PROSITEi PS50109. HIS_KIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A Bacillus subtilis regulatory gene product for genetic competence and sporulation resembles sensor protein members of the bacterial two-component signal-transduction systems."
      Weinrauch Y., Penchev R., Dubnau E., Smith I., Dubnau D.
      Genes Dev. 4:860-872(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BD630.
    2. "Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai)."
      Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H., Duesterhoeft A., Pohl T.M., Weitzenegger T.
      Microbiology 143:2769-2774(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    4. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
      Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
      Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO 64; 604 AND 654.
    5. "Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis."
      Weinrauch Y., Msadek T., Kunst F., Dubnau D.
      J. Bacteriol. 173:5685-5693(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16.
      Strain: 168.
    6. "Specificity and genetic polymorphism of the Bacillus competence quorum-sensing system."
      Tortosa P., Logsdon L., Kraigher B., Itoh Y., Mandic-Mulec I., Dubnau D.
      J. Bacteriol. 183:451-460(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: POLYMORPHISM IN COMX; COMQ AND COMP.
    7. "Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis."
      Comella N., Grossman A.D.
      Mol. Microbiol. 57:1159-1174(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiCOMP_BACSU
    AccessioniPrimary (citable) accession number: Q99027
    Secondary accession number(s): O05226
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 7, 2009
    Last modified: October 1, 2014
    This is version 112 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The DNA sequences encoding comQ, comX and the N-terminal two-thirds of comP show a striking polymorphism, which determines the specificity of the quorum-sensing system in the different pherotypes of Bacillus.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3