Skip Header

 
Contribute Send feedback

Reviewed, UniProtKB/Swiss-Prot Q99027 (COMP_BACSU)

Last modified September 2, 2008. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Sensor protein comP
    EC=2.7.13.3
Gene names
Name: comP
Ordered Locus Names: BSU31690
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Hide | Top

Protein attributes

Sequence length769 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

Hide | Top

General annotation (Comments)

Function

Sensor in the two-component regulatory system comP/comA involved in a major quorum response pathway that regulates the development of genetic competence. Plays a role in sporulation, at least partly interchangeable with that of spoIIJ.

Catalytic activity

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Post-translational modification

Autophosphorylates on a histidine and transfers the phosphate group onto an aspartate in comA, thus activating it.

Miscellaneous

The DNA sequences encoding comQ, comX and the N-terminal two-thirds of comP show a striking polymorphism, which determines the specificity of the quorum-sensing system in the different pherotypes of Bacillus.

Sequence similarities

Contains 1 histidine kinase domain.

Hide | Top

Ontologies

Keywords

   Biological processCompetence
Sporulation
Two-component regulatory system
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
   Molecular functionKinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome

Gene Ontology (GO)

None. [Check GOA]

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 769769Sensor protein comP

Regions

Topological domain1 – 99Cytoplasmic Potential
Transmembrane10 – 3324 Potential
Topological domain34 – 11380Extracellular Potential
Transmembrane114 – 13421 Potential
Topological domain135 – 14410Cytoplasmic Potential
Transmembrane145 – 16723 Potential
Topological domain168 – 23568Extracellular Potential
Transmembrane236 – 25722 Potential
Topological domain258 – 27215Cytoplasmic Potential
Transmembrane273 – 29523 Potential
Topological domain296 – 2994Extracellular Potential
Transmembrane300 – 32324 Potential
Topological domain324 – 33714Cytoplasmic Potential
Transmembrane338 – 35720 Potential
Topological domain358 – 3614Extracellular Potential
Transmembrane362 – 38322 Potential
Topological domain384 – 769386Cytoplasmic Potential
Domain571 – 769199Histidine kinase

Amino acid modifications

Modified residue4561Phosphohistidine; by autocatalysis By similarity

Experimental info

Sequence conflict6041S → C in CAA37957 and AAA22319. Ref.1
Sequence conflict6101D → Y in CAA37957 and AAA22319. Ref.1
Sequence conflict6281E → G in CAA37957 and AAA22319. Ref.1
Sequence conflict636 – 6372QL → PV in CAA37957 and AAA22319. Ref.1

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q99027-1 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: E5CEACCE278DE5B4

FASTA76989,317
        10         20         30         40         50         60 
MKNLIKKFTI AVIVLSILYI SYTTYISMNG IIIGTKIHKN DKSQFMIEEI SESSYGQFVG 

        70         80         90        100        110        120 
LRQADIILKI NKEKPSDKHL KWGYLSHINS LDILRSGKKI HLKDFDLVTL NRPYSFFLFV 

       130        140        150        160        170        180 
LPLFFYFLSI ICIFYILKVN KKRRSFAAYI LILLLLDISI AYISAGGPFR GHIINRYINL 

       190        200        210        220        230        240 
FTFISSPILY LQFIQRYLGE IGKTFLNRIS FLYIIPIFNL GIEFFQDYLQ VDIDFLATLN 

       250        260        270        280        290        300 
LVSFATLTLF SFSAIYLHLN KYKYAEHSFI LKLLILTNTL SFAPFLIFFV LPIIFTGNYI 

       310        320        330        340        350        360 
FPALASASLL VLIPFGLVYQ FVANKMFDIE FILGRMRYYA LLAMIPTLLI VGALVLFDVM 

       370        380        390        400        410        420 
DIQMNPVRQT VFFFVVMFAV FYFKEVMDFK FRLKRFSEKF NYQDSIFKYT QLMRGVTSLQ 

       430        440        450        460        470        480 
QVFKELKNTI LDVLLVSKAY TFEVTPDHKV IFLDKHEVGP DWNFYQEEFE NVTSEIGKII 

       490        500        510        520        530        540 
EVNQGFLMKV GERGGSSYVL LCLSNINTPR LTRDEISWLK TLSFYTSVSM ENVLHIEELM 

       550        560        570        580        590        600 
EHLKDLKQEG TNPIWLKKLM FAIEEKQRSG LARDLHDSVL QDLISLKRQC ELFLGDFKKD 

       610        620        630        640        650        660 
DNPSREEVQD KLVQMNEQMS DVISMTRETC HELRPQLLYD LGLVKALSKL VAHEQERVPF 

       670        680        690        700        710        720 
HIRLNTGRFT ASLDLDSQLN LYRIIQEFLS NAVKHSQATD VLIMLISIQN KIVLHYEDDG 

       730        740        750        760 
VGFDQEKNTE HSMSMGLSGI KERVRALDGR LRIETSEGKG FKADIEIEL

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"A Bacillus subtilis regulatory gene product for genetic competence and sporulation resembles sensor protein members of the bacterial two-component signal-transduction systems."
Weinrauch Y., Penchev R., Dubnau E., Smith I., Dubnau D.
Genes Dev. 4:860-872(1990) [PubMed: 2116363] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BD630.
[2]"Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai)."
Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H., Duesterhoeft A., Pohl T.M., Weitzenegger T.
Microbiology 143:2769-2774(1997) [PubMed: 9274030] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis."
Weinrauch Y., Msadek T., Kunst F., Dubnau D.
J. Bacteriol. 173:5685-5693(1991) [PubMed: 1715859] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16.
Strain: 168.
[5]"Specificity and genetic polymorphism of the Bacillus competence quorum-sensing system."
Tortosa P., Logsdon L., Kraigher B., Itoh Y., Mandic-Mulec I., Dubnau D.
J. Bacteriol. 183:451-460(2001) [PubMed: 11133937] [Abstract]
Cited for: POLYMORPHISM IN COMX; COMQ AND COMP.
[6]"Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis."
Comella N., Grossman A.D.
Mol. Microbiol. 57:1159-1174(2005) [PubMed: 16091051] [Abstract]
Cited for: FUNCTION.

Hide | Top

Cross-references

Sequence databases

X54010 mRNA. Translation: CAA37957.1.
Z93932 Genomic DNA. Translation: CAB07903.1.
Z99120 Genomic DNA. Translation: CAB15157.1.
M22856 Genomic DNA. Translation: AAA22319.1.
M71283 Genomic DNA. Translation: AAA22324.1.
PIRA35848.
B69604.
RefSeqNP_391047.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID938866.
GenomeReviewsGene locus BSU31690 in contig AL009126_GR.
KEGGbsu:BSU31690.
NMPDRfig|224308.1.peg.3172.

Organism-specific databases

SubtiListBG10380. comP. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMQ99027.

Enzyme and pathway databases

BioCycBSUB224308:BSU3164-MON.

Family and domain databases

InterProIPR003594. ATP_bd_ATPase.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
IPR005467. Sig_transdc_His_kinase_core.
[Graphical view]
Gene3DG3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
PfamPF02518. HATPase_c. 1 hit.
PF07730. HisKA_3. 1 hit.
[Graphical view]
SMARTSM00387. HATPase_c. 1 hit.
[Graphical view]
PROSITEPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProDomQ99027.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

Hide | Top

Entry information

Entry nameCOMP_BACSU
AccessionPrimary (citable) accession number: Q99027
Secondary accession number(s): O05226
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: May 30, 2000
Last modified: September 2, 2008
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents