Reviewed, UniProtKB/Swiss-Prot Q99024 (AGUA_TRIRE)

Names and origin

Protein names	Recommended name:     Alpha-glucuronidase     EC=3.2.1.139 Alternative name(s):     Alpha-glucosiduronase     GLRI
Organism	Trichoderma reesei (Hypocrea jecorina)
Taxonomic identifier	51453 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Hypocreaceae › Hypocrea

Protein attributes

Sequence length	847 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Releases 4-O-methylglucuronic acid from xylan.
Catalytic activity	An alpha-D-glucuronoside + H2O = an alcohol + D-glucuronate.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 67 family.

Ontologies

Keywords
Biological process	Xylan degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-glucuronidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 847	828	Alpha-glucuronidase		PRO_0000012244
Amino acid modifications
	Glycosylation	52	1	N-linked (GlcNAc...) Potential
	Glycosylation	238	1	N-linked (GlcNAc...) Potential
	Glycosylation	321	1	N-linked (GlcNAc...) Potential
	Glycosylation	353	1	N-linked (GlcNAc...) Potential
	Glycosylation	586	1	N-linked (GlcNAc...) Potential
	Glycosylation	692	1	N-linked (GlcNAc...) Potential
	Glycosylation	740	1	N-linked (GlcNAc...) Potential
	Glycosylation	767	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q99024-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 214AAE487382FDB4	FASTA	847	93,424
	10 20 30 40 50 60 MVIRSLLLLL LAAIVPVFAE SGIDAWLRYA RLPSSATRGH LTSFPDRIVV LNASKNGPLA 70 80 90 100 110 120 SASSELHKGI KGILGLDLDV SSRGGKHCST QKSIVISTLD TYQSACGKLS PKLNLKEDGY 130 140 150 160 170 180 WLSTKGGSVQ IIGQNERGAL YGAFQYLSYL GQGDFSGKAF ASNPSAPVRW SNQWDNLNAA 190 200 210 220 230 240 TAAHGSIERG YGGPSIFFEN GLIKEDLSRV PLYGRLLASV GLNGIVINNV NADANLLNET 250 260 270 280 290 300 NLQGLKRIAD LFRPWGVNVG ISLNFASPQV LGDLSTFDPL DDSVIKWWTD KTDRIYQLVP 310 320 330 340 350 360 DLAGYLVKAN SEGQPGPLTY NRTLAEGANL FAKAVQPHGG IVVFRAFVYD QLNETDWKAD 370 380 390 400 410 420 RANAAVDFFK SLDGQFDDNV LVQIKYGPID FQVREPASPL FANLPKTAVS IELEVTQEYL 430 440 450 460 470 480 GQQSHLVYLP PLWQTVLGFD MRYNNRQSYV RDIISGEVFG HKLGGYAGVI NVGMDDTWLG 490 500 510 520 530 540 SHLAMSNMFA YGRLAWNPRA DSRDIVEEWT RLTFGLDRDV VSTIADMSLK SWPAYEGYSG 550 560 570 580 590 600 NLGIQTLTDI LYTHYGANPA SQDNNGWGQW TRADSKTIGM DRTVSNGTGN AGQYPKEVAA 610 620 630 640 650 660 RFEHTQTTPD DLMLWFHHVP YTFRLHSGKS VIQHFYDAHY TGAATVQRFP AAWKSLKSKI 670 680 690 700 710 720 DTERYNAVLY KLQYQTGHSL VWRDAITEFY RNLSSIPDQL NRVRNHPHRI EAEDMDLSGF 730 740 750 760 770 780 TVVNVSPTEC ASKYKAIATN GTGTATTRLN VPSGKYTVAV NYYDVINGTA SYDVLLNGKS 790 800 810 820 830 840 LGKWKGDSET HLGHDFSTFL DCHSAIRITF EGVRISRGDK LTIRGTGNAQ EQAAIDYVSI LPQGVVD

References

[1]	"The alpha-glucuronidase-encoding gene of Trichoderma reesei." Margolles-Clark E., Saloheimo M., Siika-Aho M., Penttilae M. Gene 172:171-172(1996) [PubMed: 8654984] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: QM9414 / Rut C-30.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z68706 mRNA. Translation: CAA92949.1.
PIR	JC4836.
3D structure databases
HSSP	HSSP built from PDB template 1L8N based on UniProtKB Q8VVD2.
ModBase	Search...
Protein family/group databases
CAZy	GH67. Glycoside Hydrolase Family 67.
Enzyme and pathway databases
BRENDA	3.2.1.139. 280374.
Family and domain databases
InterPro	IPR011395. Alpha-glucuronds. IPR005154. Glyco_hydro_67. IPR011099. Glyco_hydro_67_C. IPR011100. Glyco_hydro_67_M. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.90.1330.10. Glyco_hydro_67_C. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF07477. Glyco_hydro_67C. 1 hit. PF07488. Glyco_hydro_67M. 1 hit. PF03648. Glyco_hydro_67N. 1 hit. [Graphical view]
PIRSF	PIRSF029900. Alpha-glucuronds. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AGUA_TRIRE

Accession

Primary (citable) accession number: Q99024

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)