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Q99020

- ROAA_MOUSE

UniProt

Q99020 - ROAA_MOUSE

Protein

Heterogeneous nuclear ribonucleoprotein A/B

Gene

Hnrnpab

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Transcriptional repressor. Binds to CArG box motifs, single-stranded and double-stranded DNA, and RNA. It may be that repression by CBF-A is a result of competitive binding of CBF, a putative positive factor, and CBF-A to the same or overlapping motifs around the CArG boxes.

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. nucleotide binding Source: InterPro
    3. protein binding Source: HGNC
    4. RNA binding Source: MGI

    GO - Biological processi

    1. epithelial to mesenchymal transition Source: HGNC
    2. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    3. positive regulation of transcription, DNA-templated Source: HGNC
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heterogeneous nuclear ribonucleoprotein A/B
    Short name:
    hnRNP A/B
    Alternative name(s):
    CArG-binding factor-A
    Short name:
    CBF-A
    Gene namesi
    Name:Hnrnpab
    Synonyms:Cbf-a, Cgbfa, Hnrpab
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1330294. Hnrnpab.

    Subcellular locationi

    Nucleus Curated. Cytoplasm By similarity
    Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: HGNC
    2. nucleus Source: HGNC
    3. ribonucleoprotein complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 285285Heterogeneous nuclear ribonucleoprotein A/BPRO_0000081493Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei220 – 2201N6-acetyllysine1 Publication
    Modified residuei247 – 2471PhosphoserineBy similarity
    Modified residuei250 – 2501Dimethylated arginine; alternateBy similarity
    Modified residuei250 – 2501Omega-N-methylarginine; alternateBy similarity
    Modified residuei271 – 2711N6-acetyllysine1 Publication
    Modified residuei275 – 2751Dimethylated arginineBy similarity

    Keywords - PTMi

    Acetylation, Methylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ99020.
    PaxDbiQ99020.
    PRIDEiQ99020.

    PTM databases

    PhosphoSiteiQ99020.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Gene expression databases

    ArrayExpressiQ99020.
    BgeeiQ99020.
    GenevestigatoriQ99020.

    Interactioni

    Subunit structurei

    Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with APOBEC1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi200358. 6 interactions.
    DIPiDIP-31415N.
    IntActiQ99020. 9 interactions.
    MINTiMINT-4132837.

    Structurei

    3D structure databases

    ProteinModelPortaliQ99020.
    SMRiQ99020. Positions 70-237.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini75 – 15884RRM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini159 – 23880RRM 2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi34 – 4310Poly-Ala
    Compositional biasi246 – 27732Gly-richAdd
    BLAST

    Sequence similaritiesi

    Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0724.
    GeneTreeiENSGT00730000110184.
    HOGENOMiHOG000234441.
    HOVERGENiHBG002295.
    KOiK13044.
    OrthoDBiEOG715Q6V.

    Family and domain databases

    Gene3Di3.30.70.330. 2 hits.
    InterProiIPR012956. CARG-binding_factor_N.
    IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view]
    PfamiPF08143. CBFNT. 1 hit.
    PF00076. RRM_1. 2 hits.
    [Graphical view]
    SMARTiSM00360. RRM. 2 hits.
    [Graphical view]
    PROSITEiPS50102. RRM. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q99020-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDAAEEQPM ETTGATENGH EAAPEGEAPV EPSAAAAAPA ASAGSGGGTT    50
    TAPSGNQNGA EGDQINASKN EEDAGKMFVG GLSWDTSKKD LKDYFTKFGE 100
    VVDCTIKMDP NTGRSRGFGF ILFKDSSSVE KVLDQKEHRL DGRVIDPKKA 150
    MAMKKDPVKK IFVGGLNPEA TEEKIREYFG QFGEIEAIEL PIDPKLNKRR 200
    GFVFITFKEE DPVKKVLEKK FHTVSGSKCE IKVAQPKEVY QQQQYGSGGR 250
    GNRNRGNRGS GGGQGSTNYG KSQRRGGHQN NYKPY 285
    Length:285
    Mass (Da):30,831
    Last modified:February 1, 1995 - v1
    Checksum:i7881F34131160E0C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 261G → S in BAC36208. (PubMed:16141072)Curated
    Sequence conflicti41 – 411A → R in BAC36208. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90151 mRNA. Translation: BAA14181.1.
    L36663 mRNA. Translation: AAA92146.1.
    AK076132 mRNA. Translation: BAC36208.1.
    CCDSiCCDS24654.1.
    PIRiJQ0448.
    RefSeqiNP_034578.1. NM_010448.3.
    UniGeneiMm.256875.
    Mm.280842.

    Genome annotation databases

    EnsembliENSMUST00000074669; ENSMUSP00000074238; ENSMUSG00000020358.
    GeneIDi15384.
    KEGGimmu:15384.
    UCSCiuc007iua.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90151 mRNA. Translation: BAA14181.1 .
    L36663 mRNA. Translation: AAA92146.1 .
    AK076132 mRNA. Translation: BAC36208.1 .
    CCDSi CCDS24654.1.
    PIRi JQ0448.
    RefSeqi NP_034578.1. NM_010448.3.
    UniGenei Mm.256875.
    Mm.280842.

    3D structure databases

    ProteinModelPortali Q99020.
    SMRi Q99020. Positions 70-237.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200358. 6 interactions.
    DIPi DIP-31415N.
    IntActi Q99020. 9 interactions.
    MINTi MINT-4132837.

    PTM databases

    PhosphoSitei Q99020.

    Proteomic databases

    MaxQBi Q99020.
    PaxDbi Q99020.
    PRIDEi Q99020.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000074669 ; ENSMUSP00000074238 ; ENSMUSG00000020358 .
    GeneIDi 15384.
    KEGGi mmu:15384.
    UCSCi uc007iua.1. mouse.

    Organism-specific databases

    CTDi 3182.
    MGIi MGI:1330294. Hnrnpab.

    Phylogenomic databases

    eggNOGi COG0724.
    GeneTreei ENSGT00730000110184.
    HOGENOMi HOG000234441.
    HOVERGENi HBG002295.
    KOi K13044.
    OrthoDBi EOG715Q6V.

    Miscellaneous databases

    ChiTaRSi HNRNPAB. mouse.
    NextBioi 288056.
    PROi Q99020.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q99020.
    Bgeei Q99020.
    Genevestigatori Q99020.

    Family and domain databases

    Gene3Di 3.30.70.330. 2 hits.
    InterProi IPR012956. CARG-binding_factor_N.
    IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    [Graphical view ]
    Pfami PF08143. CBFNT. 1 hit.
    PF00076. RRM_1. 2 hits.
    [Graphical view ]
    SMARTi SM00360. RRM. 2 hits.
    [Graphical view ]
    PROSITEi PS50102. RRM. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A protein binding to CArG box motifs and to single-stranded DNA functions as a transcriptional repressor."
      Kamada S., Miwa T.
      Gene 119:229-236(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Muscle.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
    3. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 117-124; 161-174; 177-195 AND 201-208, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-220 AND LYS-271, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiROAA_MOUSE
    AccessioniPrimary (citable) accession number: Q99020
    Secondary accession number(s): Q8BPE0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3