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Protein

Heterogeneous nuclear ribonucleoprotein A/B

Gene

Hnrnpab

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor. Binds to CArG box motifs, single-stranded and double-stranded DNA, and RNA. It may be that repression by CBF-A is a result of competitive binding of CBF, a putative positive factor, and CBF-A to the same or overlapping motifs around the CArG boxes.

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: MGI
  • RNA binding Source: MGI

GO - Biological processi

  • epithelial to mesenchymal transition Source: HGNC
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of transcription, DNA-templated Source: HGNC
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein A/B
Short name:
hnRNP A/B
Alternative name(s):
CArG-binding factor-A
Short name:
CBF-A
Gene namesi
Name:Hnrnpab
Synonyms:Cbf-a, Cgbfa, Hnrpab
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1330294. Hnrnpab.

Subcellular locationi

  • Nucleus Curated
  • Cytoplasm By similarity

  • Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.By similarity

GO - Cellular componenti

  • cytoplasm Source: HGNC
  • intracellular ribonucleoprotein complex Source: UniProtKB
  • nucleoplasm Source: MGI
  • nucleus Source: HGNC
  • RNA polymerase II transcription factor complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000814931 – 285Heterogeneous nuclear ribonucleoprotein A/BAdd BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei87PhosphoserineBy similarity1
Modified residuei220N6-acetyllysineCombined sources1
Modified residuei247PhosphoserineBy similarity1
Modified residuei250Dimethylated arginine; alternateBy similarity1
Modified residuei250Omega-N-methylarginine; alternateCombined sources1
Modified residuei255Omega-N-methylarginineCombined sources1
Modified residuei258Omega-N-methylarginineCombined sources1
Modified residuei271N6-acetyllysineCombined sources1
Modified residuei275Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei275Dimethylated arginine; alternateBy similarity1
Modified residuei275Omega-N-methylarginine; alternateCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ99020.
MaxQBiQ99020.
PRIDEiQ99020.

PTM databases

iPTMnetiQ99020.
PhosphoSitePlusiQ99020.
SwissPalmiQ99020.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSMUSG00000020358.
ExpressionAtlasiQ99020. baseline and differential.
GenevisibleiQ99020. MM.

Interactioni

Subunit structurei

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with APOBEC1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi200358. 8 interactors.
DIPiDIP-31415N.
IntActiQ99020. 9 interactors.
MINTiMINT-4132837.

Structurei

3D structure databases

ProteinModelPortaliQ99020.
SMRiQ99020.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini75 – 158RRM 1PROSITE-ProRule annotationAdd BLAST84
Domaini159 – 238RRM 2PROSITE-ProRule annotationAdd BLAST80

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi34 – 43Poly-Ala10
Compositional biasi246 – 277Gly-richAdd BLAST32

Sequence similaritiesi

Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00760000118873.
HOGENOMiHOG000234441.
HOVERGENiHBG002295.
InParanoidiQ99020.
KOiK13044.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012956. CARG-binding_factor_N.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF08143. CBFNT. 1 hit.
PF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q99020-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDAAEEQPM ETTGATENGH EAAPEGEAPV EPSAAAAAPA ASAGSGGGTT
60 70 80 90 100
TAPSGNQNGA EGDQINASKN EEDAGKMFVG GLSWDTSKKD LKDYFTKFGE
110 120 130 140 150
VVDCTIKMDP NTGRSRGFGF ILFKDSSSVE KVLDQKEHRL DGRVIDPKKA
160 170 180 190 200
MAMKKDPVKK IFVGGLNPEA TEEKIREYFG QFGEIEAIEL PIDPKLNKRR
210 220 230 240 250
GFVFITFKEE DPVKKVLEKK FHTVSGSKCE IKVAQPKEVY QQQQYGSGGR
260 270 280
GNRNRGNRGS GGGQGSTNYG KSQRRGGHQN NYKPY
Length:285
Mass (Da):30,831
Last modified:February 1, 1995 - v1
Checksum:i7881F34131160E0C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26G → S in BAC36208 (PubMed:16141072).Curated1
Sequence conflicti41A → R in BAC36208 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90151 mRNA. Translation: BAA14181.1.
L36663 mRNA. Translation: AAA92146.1.
AK076132 mRNA. Translation: BAC36208.1.
CCDSiCCDS24654.1.
PIRiJQ0448.
RefSeqiNP_034578.1. NM_010448.3.
UniGeneiMm.256875.
Mm.280842.

Genome annotation databases

EnsembliENSMUST00000074669; ENSMUSP00000074238; ENSMUSG00000020358.
GeneIDi15384.
KEGGimmu:15384.
UCSCiuc007iua.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90151 mRNA. Translation: BAA14181.1.
L36663 mRNA. Translation: AAA92146.1.
AK076132 mRNA. Translation: BAC36208.1.
CCDSiCCDS24654.1.
PIRiJQ0448.
RefSeqiNP_034578.1. NM_010448.3.
UniGeneiMm.256875.
Mm.280842.

3D structure databases

ProteinModelPortaliQ99020.
SMRiQ99020.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200358. 8 interactors.
DIPiDIP-31415N.
IntActiQ99020. 9 interactors.
MINTiMINT-4132837.

PTM databases

iPTMnetiQ99020.
PhosphoSitePlusiQ99020.
SwissPalmiQ99020.

Proteomic databases

EPDiQ99020.
MaxQBiQ99020.
PRIDEiQ99020.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000074669; ENSMUSP00000074238; ENSMUSG00000020358.
GeneIDi15384.
KEGGimmu:15384.
UCSCiuc007iua.1. mouse.

Organism-specific databases

CTDi3182.
MGIiMGI:1330294. Hnrnpab.

Phylogenomic databases

GeneTreeiENSGT00760000118873.
HOGENOMiHOG000234441.
HOVERGENiHBG002295.
InParanoidiQ99020.
KOiK13044.

Miscellaneous databases

ChiTaRSiHnrnpab. mouse.
PROiQ99020.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020358.
ExpressionAtlasiQ99020. baseline and differential.
GenevisibleiQ99020. MM.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012956. CARG-binding_factor_N.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF08143. CBFNT. 1 hit.
PF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiROAA_MOUSE
AccessioniPrimary (citable) accession number: Q99020
Secondary accession number(s): Q8BPE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.