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Protein

Ice-structuring protein B

Gene
N/A
Organism
Pseudopleuronectes americanus (Winter flounder) (Pleuronectes americanus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to protect fish blood from freezing at subzero sea water temperatures. Lowers the blood freezing point. Binds to nascent ice crystals and prevents further growth.1 Publication

GO - Molecular functioni

  • ice binding Source: UniProtKB

GO - Biological processi

  • homoiothermy Source: UniProtKB
  • response to freezing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antifreeze protein

Names & Taxonomyi

Protein namesi
Recommended name:
Ice-structuring protein B
Short name:
ISP B
Alternative name(s):
Antifreeze protein B
HPLC8
OrganismiPseudopleuronectes americanus (Winter flounder) (Pleuronectes americanus)
Taxonomic identifieri8265 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataCarangariaPleuronectiformesPleuronectoideiPleuronectidaePseudopleuronectes

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
PropeptideiPRO_000022559624 – 44Removed by a dipeptidylpeptidase1 PublicationAdd BLAST21
ChainiPRO_000022559745 – 81Ice-structuring protein B1 PublicationAdd BLAST37

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei81Arginine amideBy similarity1

Post-translational modificationi

Amidated.1 Publication

Keywords - PTMi

Amidation

Expressioni

Tissue specificityi

Detected in liver (at protein level).1 Publication

Inductioni

By winter conditions, at least in part by water temperatures of below 8 degrees Celsius.1 Publication

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi28 – 80Ala-richSequence analysisAdd BLAST53

Sequence similaritiesi

Belongs to the type-I AFP family.Sequence analysis

Keywords - Domaini

Repeat, Signal

Family and domain databases

InterProiIPR000104. Antifreeze_1.
[Graphical view]
PRINTSiPR00308. ANTIFREEZEI.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q99013-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSLFTVGQ LIFLFWTMRI TEARPDPAAK AAPAAAAVPA AAAPDTASDA
60 70 80
AAAAALTAAN AAAAAKLTAD NAAAAAAATA RG
Length:82
Mass (Da):7,794
Last modified:November 1, 1996 - v1
Checksum:iC8AEDA75DA4B87FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62413 Genomic DNA. Translation: AAA49468.1.
PIRiJS0705.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62413 Genomic DNA. Translation: AAA49468.1.
PIRiJS0705.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR000104. Antifreeze_1.
[Graphical view]
PRINTSiPR00308. ANTIFREEZEI.
ProtoNetiSearch...

Entry informationi

Entry nameiANPB_PSEAM
AccessioniPrimary (citable) accession number: Q99013
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.