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Q98UI9 (MUC5B_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mucin-5B
Alternative name(s):
Ovomucin, alpha-subunit
Gene names
Name:MUC5B
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length2108 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ovomucin, the glycoprotein responsible for the gel properties of egg white, is composed for 2 subunits, alpha-ovomucin/MUC5B and beta-ovomucin/MUC6. Ref.2

Subunit structure

Multimer; disulfide-linked. Ref.2

Subcellular location

Secreted.

Post-translational modification

N-glycosylated. Complex glycosylation with bisecting N-acetylglucosamine. Contains mainly N-acetylglucosamine (3.1-8.5%), mannose (2.9-4.6%), a small amount of galactose (1.1-4.35) and sialic acid (0.3-1.3%). Most abundant glycan is composed of a GlcNAc2Man3 core, a bisecting GlcNAc and another 3 GlcNAc antannae located on the mannoses of the core. Site Asn-1639 exists both in glycosylated and non-glycosylated forms. Ref.2 Ref.3

Sequence similarities

Contains 1 CTCK (C-terminal cystine knot-like) domain.

Contains 3 TIL (trypsin inhibitory-like) domains.

Contains 3 VWFC domains.

Contains 4 VWFD domains.

Ontologies

Keywords
   Cellular componentSecreted
   DomainRepeat
Signal
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 21082087Mucin-5B
PRO_5000049585

Regions

Domain37 – 242206VWFD 1
Domain304 – 36057TIL 1
Domain399 – 610212VWFD 2
Domain666 – 72358TIL 2
Domain782 – 82544TIL 3
Domain825 – 89773VWFC 1
Domain864 – 1069206VWFD 3
Domain1430 – 1646217VWFD 4
Domain1761 – 183272VWFC 2
Domain1870 – 193768VWFC 3
Domain2010 – 210495CTCK

Sites

Site691Not glycosylated
Site6731Not glycosylated

Amino acid modifications

Glycosylation3811N-linked (GlcNAc...) (complex) Ref.3
Glycosylation5281N-linked (GlcNAc...) (complex) Ref.3
Glycosylation5991N-linked (GlcNAc...) (complex) Ref.3
Glycosylation6801N-linked (GlcNAc...) (complex) Ref.3
Glycosylation7721N-linked (GlcNAc...) (complex) Ref.3
Glycosylation8551N-linked (GlcNAc...) (complex) Ref.3
Glycosylation10361N-linked (GlcNAc...) (complex) Ref.3
Glycosylation12191N-linked (GlcNAc...) (complex) Ref.3
Glycosylation13711N-linked (GlcNAc...) (complex) Ref.3
Glycosylation14521N-linked (GlcNAc...) (complex) Ref.3
Glycosylation15671N-linked (GlcNAc...) (complex) Ref.3
Glycosylation16391N-linked (GlcNAc...) (complex) Ref.3
Glycosylation17921N-linked (GlcNAc...) (complex) Ref.3
Glycosylation18071N-linked (GlcNAc...) (complex) Ref.3
Glycosylation18411N-linked (GlcNAc...) (complex) Ref.3
Glycosylation19641N-linked (GlcNAc...) (complex) Ref.3
Disulfide bond60 ↔ 68 By similarity
Disulfide bond2010 ↔ 2066 By similarity
Disulfide bond2031 ↔ 2080 By similarity
Disulfide bond2042 ↔ 2096 By similarity
Disulfide bond2046 ↔ 2098 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q98UI9 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 68B887CB781E6539

FASTA2,108233,553
        10         20         30         40         50         60 
MEIKKERSFW IFCLIWSFCK GKEPVQIVQV STVGRSECTT WGNFHFHTFD HVKFTFPGTC 

        70         80         90        100        110        120 
TYVFASHCND SYQDFNIKIR RSDKNSHLIY FTVTTDGVIL EVKETGITVN GNQIPLPFSL 

       130        140        150        160        170        180 
KSILIEDTCA YFQVTSKLGL TLKWNWADTL LLDLEETYKE KICGLCGNYD GNKKNDLILD 

       190        200        210        220        230        240 
GYKMHPRQFG NFHKVEDPSE KCPDVRPDDH TGRHPTEDDN RCSKYKKMCK KLLSRFGNCP 

       250        260        270        280        290        300 
KVVAFDDYVA TCTEDMCNCV VNSSQSDLVS SCICSTLNQY SRDCVLSKGD PGEWRTKELC 

       310        320        330        340        350        360 
YQECPSNMEY MECGNSCADT CADPERSKIC KAPCTDGCFC PPGTILDDLG GKKCVPRDSC 

       370        380        390        400        410        420 
PCMFQGKVYS SGGTYSTPCQ NCTCKGGHWS CISLPCSGSC SIDGGFHIKT FDNKKFNFHG 

       430        440        450        460        470        480 
NCHYVLAKNT DDTFVVIGEI IQCGTSKTMT CLKNVLVTLG RTTIKICSCG SIYMNNFIVK 

       490        500        510        520        530        540 
LPVSKDGITI FRPSTFFIKI LSSAGVQIRV QMKPVMQLSI TVDHSYQNRT SGLCGNFNNI 

       550        560        570        580        590        600 
QTDDFRTATG AVEDSAAAFG NSWKTRASCF DVEDSFEDPC SNSVDKEKFA QHWCALLSNT 

       610        620        630        640        650        660 
SSTFAACHSV VDPSVYIKRC MYDTCNAEKS EVALCSVLST YSRDCAAAGM TLKGWRQGIC 

       670        680        690        700        710        720 
DPSEECPETM VYNYSVKYCN QSCRSLDEPD PLCKVQIAPM EGCGCPEGTY LNDEEECVTP 

       730        740        750        760        770        780 
DDCPCYYKGK IVQPGNSFQE DKLLCKCIQG RLDCIGETVL VKDCPAPMYY FNCSSAGPGA 

       790        800        810        820        830        840 
IGSECQKSCK TQDMHCYVTE CVSGCMCPDG LVLDGSGGCI PKDQCPCVHG GHFYKPGETI 

       850        860        870        880        890        900 
RVDCNTCTCN KRQWNCTDNP CKGTCTVYGN GHYMSFDGEK FDFLGDCDYI LAQDFCPNNM 

       910        920        930        940        950        960 
DAGTFRIVIQ NNACGKSLSI CSLKITLIFE SSEIRLLEGR IQEIATDPGA EKNYKVDLRG 

       970        980        990       1000       1010       1020 
GYIVIETTQG MSFMWDQKTT VVVHVTPSFQ GKVCGLCGDF DGRSRNDFTT RGQSVEMSIQ 

      1030       1040       1050       1060       1070       1080 
EFGNSWKITS TCSNINMTDL CADQPFKSAL GQKHCSIIKS SVFEACHSKV NPIPYYESCV 

      1090       1100       1110       1120       1130       1140 
SDFCGCDSVG DCECFCTSVA AYARSCSTAG VCINWRTPAI CPVFCDYYNP PDKHEWFYKP 

      1150       1160       1170       1180       1190       1200 
CGAPCLKTCR NPQGKCGNIL YSLEGCYPEC SPDKPYFDEE RRECVSLPDC TSCNPEEKLC 

      1210       1220       1230       1240       1250       1260 
TEDSKDCLCC YNGKTYPLNE TIYSQTEGTK CGNAFCGPNG MIIETFIPCS TLSVPAQEQL 

      1270       1280       1290       1300       1310       1320 
MQPVTSAPLL STEATPCFCT DNGQLIQMGE NVSLPMNISG HCAYSICNAS CQIELIWAEC 

      1330       1340       1350       1360       1370       1380 
KVVQTEALET CEPNSEACPP TAAPNATSLV PATALAPMSD CLGLIPPRKF NESWDFGNCQ 

      1390       1400       1410       1420       1430       1440 
IATCLGEENN IKLSSITCPP QQLKLCVNGF PFMKHHDETG CCEVFECQCI CSGWGNEHYV 

      1450       1460       1470       1480       1490       1500 
TFDGTYYHFK ENCTYVLVEL IQPSSEKFWI HIDNYYCGAA DGAICSMSLL IFHSNSLVIL 

      1510       1520       1530       1540       1550       1560 
TQAKEHGKGT NLVLFNDKKV VPDISKNGIR ITSSGLYIIV EIPELEVYVS YSRLAFYIKL 

      1570       1580       1590       1600       1610       1620 
PFGKYYNNTM GLCGTCTNQK SDDARKRNGE VTDSFKEMAL DWKAPVSTNR YCNPGISEPV 

      1630       1640       1650       1660       1670       1680 
KIENYQHCEP SELCKIIWNL TECHRVVPPQ PYYEACVASR CSQQHPSTEC QSMQTYAALC 

      1690       1700       1710       1720       1730       1740 
GLHGICVDWR GQTNGQCEAT CARDQVYKPC GEAKRNTCFS REVIVDTLLS RNNTPVFVEG 

      1750       1760       1770       1780       1790       1800 
CYCPDGNILL NEHDGICVSV CGCTAQDGSV KKPREAWEHD CQYCTCDEET LNISCFPRPC 

      1810       1820       1830       1840       1850       1860 
AKSPPINCTK EGFVRKIKPR LDDPCCTETV CECDIKTCII NKTACDLGFQ PVVAISEDGC 

      1870       1880       1890       1900       1910       1920 
CPIFSCIPKG VCVSEGVEFK PGAVVPKSSC EDCVCTDEQD AVTGTNRIQC VPVKCQTTCQ 

      1930       1940       1950       1960       1970       1980 
QGFRYVEKEG QCCSQCQQVA CVANFPFGSV TIEVGKSYKA PYDNCTQYTC TESGGQFSLT 

      1990       2000       2010       2020       2030       2040 
STVKVCLPFE ESNCVPGTVD VTSDGCCKTC IDLPHKCKRS MKEQYIVHKH CKSAAPVPVP 

      2050       2060       2070       2080       2090       2100 
FCEGTCSTYS VYSFENNEME HKCICCHEKK SHVEKVELVC SEHKTLKFSY VHVDECGCVE 


TKCPMRRT 

« Hide

References

[1]"Amino acid sequence of alpha-subunit in hen egg white ovomucin deduced from cloned cDNA."
Watanabe K., Shimoyamada M., Onizuka T., Akiyama H., Niwa M., Ido T., Tsuge Y.
DNA Seq. 15:251-261(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Oviduct.
[2]"Studies on the composition of egg-white ovomucin."
Robinson D.S., Monsey J.B.
Biochem. J. 121:537-547(1971) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE OF CARBOHYDRATES, FUNCTION, SUBUNIT.
[3]"N-glycosylation of ovomucin from hen egg white."
Offengenden M., Fentabil M.A., Wu J.
Glycoconj. J. 28:113-123(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT ASN-381; ASN-528; ASN-599; ASN-680; ASN-772; ASN-855; ASN-1036; ASN-1219; ASN-1371; ASN-1452; ASN-1567; ASN-1639; ASN-1792; ASN-1807; ASN-1841 AND ASN-1964, LACK OF GLYCOSYLATION AT ASN-69 AND ASN-673, IDENTIFICATION BY MASS SPECTROMETRY, STRUCTURE OF CARBOHYDRATES.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB046524 mRNA. Translation: BAB21488.1.
UniGeneGga.3337.

3D structure databases

ProteinModelPortalQ98UI9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000010852.

Protein family/group databases

Allergome2741. Gal d Ovomucin.

Proteomic databases

PaxDbQ98UI9.
PRIDEQ98UI9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGNOG12793.
HOGENOMHOG000168234.
HOVERGENHBG004380.
InParanoidQ98UI9.
PhylomeDBQ98UI9.

Family and domain databases

InterProIPR006207. Cys_knot_C.
IPR002919. TIL_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR001007. VWF_C.
IPR001846. VWF_type-D.
[Graphical view]
PfamPF08742. C8. 4 hits.
PF01826. TIL. 3 hits.
PF00094. VWD. 4 hits.
[Graphical view]
SMARTSM00832. C8. 4 hits.
SM00041. CT. 1 hit.
SM00214. VWC. 5 hits.
SM00216. VWD. 4 hits.
[Graphical view]
SUPFAMSSF57567. SSF57567. 5 hits.
PROSITEPS01225. CTCK_2. 1 hit.
PS01208. VWFC_1. 2 hits.
PS50184. VWFC_2. 2 hits.
PS51233. VWFD. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio20815465.
PROQ98UI9.

Entry information

Entry nameMUC5B_CHICK
AccessionPrimary (citable) accession number: Q98UI9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families