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Protein

RAC-alpha serine/threonine-protein kinase

Gene

akt1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

AKT1 is one of several closely related serine/threonine-protein kinases known as the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase (By similarity). Required for insulin-stimulated meiotic reinitiation during oocyte maturation.By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Enzyme regulationi

Activated in response to insulin. Three specific sites, one in the kinase domain (Thr-309) and the two other ones in the C-terminal regulatory region (Ser-474 and Tyr-475), need to be phosphorylated for its full activation.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei180 – 1801ATPPROSITE-ProRule annotation
Active sitei275 – 2751Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi157 – 1659ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism, Glycogen biosynthesis, Neurogenesis, Sugar transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RAC-alpha serine/threonine-protein kinase (EC:2.7.11.1)
Alternative name(s):
Protein kinase Akt-1
Short name:
xAkt
Protein kinase B, alpha
Short name:
PKB alpha
RAC-PK-alpha
Gene namesi
Name:akt1By similarity
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-484957. akt1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Nucleus after activation by integrin-linked protein kinase 1 (ILK1).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 481481RAC-alpha serine/threonine-protein kinaseCuratedPRO_0000223507Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi127 – 1271O-linked (GlcNAc)By similarity
Glycosylationi130 – 1301O-linked (GlcNAc)By similarity
Glycosylationi306 – 3061O-linked (GlcNAc)By similarity
Modified residuei309 – 3091Phosphothreonine; by PDPK1By similarity
Glycosylationi313 – 3131O-linked (GlcNAc)By similarity
Modified residuei474 – 4741PhosphoserineBy similarity
Modified residuei475 – 4751PhosphotyrosineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Expressioni

Tissue specificityi

Expressed in the oocyte.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ98TY9.
SMRiQ98TY9. Positions 1-116, 145-478.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 108104PHPROSITE-ProRule annotationAdd
BLAST
Domaini151 – 409259Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini410 – 48172AGC-kinase C-terminalAdd
BLAST

Domaini

Binding of the PH domain to phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P3) following phosphatidylinositol 3-kinase alpha (PIK3CA) activity results in its targeting to the plasma membrane.By similarity

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG108317.
KOiK04456.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q98TY9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEVAIVKEG WLHKRGEYIK TWRPRYFLLK SDGTFIGYKE RPQDVDQLET
60 70 80 90 100
PLNNFSVAKC QLMKTERPKP NTFIIRCLQW TTVIERTFHV DSPEEREEWI
110 120 130 140 150
QVIQHVADNL KKQEEEMMEV RSGDSPSDNS GAEEMEVSHS KPKHKVTMNE
160 170 180 190 200
FEYLKLLGKG TFGKVILVKE KATGRYYAMK ILKKEVIVAK DEVAHTLTEN
210 220 230 240 250
RVLQNSRHPF LTALKYSFQT HDRLCFVMEY ANGGELFFHL SRERIFSEDR
260 270 280 290 300
ARFYGAEIVS ALDYLHSEKN VVYRDLKLEN LMLDKDGHIK ITDFGLCKEG
310 320 330 340 350
IKDGATMKTF CGTPEYLAPE VLEDNDYGRA VDWWGLGVVM YEMMCGRLPF
360 370 380 390 400
YNQDHEKLFE LILMEEIRFP RTLLPEAKSL LSGLLKKDPK QRLGGGPDDA
410 420 430 440 450
KEIMQHKFFA GIVWQDVYEK KLVPPFKPQV TSETDTRYFD EEFTAQMITI
460 470 480
TPPDQDDNFE FVDNERRPHF PQFSYSASGN A
Length:481
Mass (Da):56,042
Last modified:June 1, 2001 - v1
Checksum:iFF56CFB9A6454303
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF317656 mRNA. Translation: AAG59601.1.
RefSeqiNP_001083878.1. NM_001090409.1.
UniGeneiXl.738.

Genome annotation databases

GeneIDi399170.
KEGGixla:399170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF317656 mRNA. Translation: AAG59601.1.
RefSeqiNP_001083878.1. NM_001090409.1.
UniGeneiXl.738.

3D structure databases

ProteinModelPortaliQ98TY9.
SMRiQ98TY9. Positions 1-116, 145-478.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi399170.
KEGGixla:399170.

Organism-specific databases

CTDi207.
XenbaseiXB-GENE-484957. akt1.

Phylogenomic databases

HOVERGENiHBG108317.
KOiK04456.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAKT1_XENLA
AccessioniPrimary (citable) accession number: Q98TY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: June 1, 2001
Last modified: May 11, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.