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Protein

Zinc metalloproteinase/disintegrin

Gene
N/A
Organism
Bothrops jararaca (Jararaca) (Bothrops jajaraca)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom metalloproteinase jararafibrase-2: impairs hemostasis in the envenomed animal (By similarity). Does not exhibit detectable plasminogen activating activity. Has hemagglutinating activity on red blood cells. Cleaves insulin B chain at '38-Ala-|-Leu-39' and '40-Tyr-|-Leu-41' bonds (PubMed:8087204).By similarity3 Publications
Disintegrin bothrostatin: this recombinant protein shows high inhibitory activity on collagen-induced platelet aggregation.

Miscellaneous

The disintegrin belongs to the medium disintegrin subfamily.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Inhibited by 1,10-phenanthroline and EDTA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi196CalciumBy similarity1
Metal bindingi280CalciumBy similarity1
Metal bindingi329Zinc; catalyticBy similarity1
Active sitei330PROSITE-ProRule annotation1
Metal bindingi333Zinc; catalyticBy similarity1
Metal bindingi339Zinc; catalyticBy similarity1
Metal bindingi384Calcium; via carbonyl oxygenBy similarity1
Metal bindingi387CalciumBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionCell adhesion impairing toxin, Hemostasis impairing toxin, Hydrolase, Metalloprotease, Platelet aggregation inhibiting toxin, Protease, Toxin
LigandCalcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc metalloproteinase/disintegrin
Cleaved into the following 2 chains:
Alternative name(s):
Jararafibrase II
Disintegrin bothrostatin
Short name:
D-BTT
OrganismiBothrops jararaca (Jararaca) (Bothrops jajaraca)
Taxonomic identifieri8724 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000032642321 – 1871 PublicationAdd BLAST167
ChainiPRO_0000326424188 – 389Snake venom metalloproteinase jararafibrase-2Add BLAST202
PropeptideiPRO_0000326425390 – 404By similarityAdd BLAST15
ChainiPRO_0000326426405 – 477Disintegrin bothrostatinAdd BLAST73

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi304 ↔ 384By similarity
Disulfide bondi344 ↔ 368By similarity
Disulfide bondi346 ↔ 351By similarity
Disulfide bondi410 ↔ 419By similarity
Disulfide bondi412 ↔ 420By similarity
Disulfide bondi425 ↔ 439By similarity
Disulfide bondi433 ↔ 463By similarity
Disulfide bondi438 ↔ 442By similarity
Disulfide bondi451 ↔ 470By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PRIDEiQ98SP2.

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ98SP2.
SMRiQ98SP2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini193 – 389Peptidase M12BPROSITE-ProRule annotationAdd BLAST197
Domaini396 – 477DisintegrinPROSITE-ProRule annotationAdd BLAST82

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi455 – 457Cell attachment site3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG006978.

Family and domain databases

CDDicd04269. ZnMc_adamalysin_II_like. 1 hit.
Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiView protein in InterPro
IPR018358. Disintegrin_CS.
IPR001762. Disintegrin_dom.
IPR036436. Disintegrin_dom_sf.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
IPR034027. Reprolysin_adamalysin.
PfamiView protein in Pfam
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
PRINTSiPR00289. DISINTEGRIN.
SMARTiView protein in SMART
SM00050. DISIN. 1 hit.
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiView protein in PROSITE
PS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q98SP2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEVLLVTIC LAAFPYQGSS IILESGNVND YEVIYPRKVT ALPKGAVQPK
60 70 80 90 100
YEDAMQYELK VNGEPVVLHL EKNKGLFSKD YSETHYSPDG RKITTNPPVE
110 120 130 140 150
DHCYYHGRIE NDADSTASIS ACNGLKGHFK LQGETYLIEP LKLSDSEAHA
160 170 180 190 200
VFKFENVEKE DEAPKMCGVT QNWESYEPIK KASQSNLTPE HQRYIELFLV
210 220 230 240 250
VDHGMFMKYN GNSDKIRRRI HQMVNIMKEA YRYLYIDIAL TGVEIWSNKD
260 270 280 290 300
MINVQPAAPQ TLDSFGEWRK TDLLNRKSHD NAQLLTSTDF KDQTIGLAYW
310 320 330 340 350
GSMCDPKRST AVIEDHSETD LLVAVTMAHE LGHNLGIRHD TGSCSCGGYS
360 370 380 390 400
CIMAPVISHD IAKYFSDCSY IQCWDFIMKD NPQCILNKQL RTDTVSTPVS
410 420 430 440 450
GKNFGAGEEC DCGTPGNPCC DAVTCKLRPG AQCAEGLCCD QCRFMKEGTV
460 470
CRRARGDDMD DYCNGISAGC PRNPFHA
Length:477
Mass (Da):53,440
Last modified:June 1, 2001 - v1
Checksum:iAC973EE7767E10B3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti188T → L AA sequence (PubMed:1523677).Curated1
Sequence conflicti199L → I AA sequence (PubMed:1523677).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF345931 mRNA. Translation: AAK15542.1.
PIRiA42766.

Similar proteinsi

Entry informationi

Entry nameiVM2J2_BOTJA
AccessioniPrimary (citable) accession number: Q98SP2
Secondary accession number(s): Q9PS47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 2001
Last modified: October 25, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families