Q98SN7 (WNT2B_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-2b

Gene names

Name:

WNT2B

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Signaling molecule which affects the development of discrete regions of tissues. Could participate in the process of blood vessel formation. Is likely to signal over only few cell diameters. May be involved in brain development and in later organogenesis. Acts as an upstream regulator of FGF10 in the lateral plate mesoderm during limb initiation in the embryo. Ref.1 Ref.2
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity.
Tissue specificity	Expressed in the intermediate and the lateral plate mesoderm in the presumptive chick forelimb region. Expressed in the eye, head ectoderm, prospective forelimb mesenchyme, lung bud, pharyngeal arches, the brain and otic vesicle. Ref.1 Ref.2 Ref.3
Developmental stage	At stage 11, expressed in the medial sites of the embryonic right and left coelom, as well as in the medial region of the forming somites. Between stages 14 and 16, expression in the presumptive wing field, with strongest expression at stage 15. At stage 17, a faint expression is detected in the posterior region of the nascent limb bud. Expressed in the ocular surface ectoderm, including the corneal epithelium, starting from the onset of the lens vesicle closing at stage 16. Ref.1 Ref.3
Post-translational modification	Palmitoylation at Ser-237 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-101. Palmitoylation is necessary for proper trafficking to cell surface By similarity.
Sequence similarities	Belongs to the Wnt family.
Caution	Was initially thought (Ref.2) to be WNT13.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway, calcium modulating pathway Inferred from Biological aspect of Ancestor. Source: RefGenome camera-type eye photoreceptor cell differentiation Inferred from expression pattern PubMed 16854977. Source: AgBase canonical Wnt receptor signaling pathway Inferred from direct assay PubMed 12490564PubMed 16854977. Source: AgBase cell proliferation Inferred from direct assay PubMed 12490564. Source: AgBase cornea development in camera-type eye Inferred from expression pattern Ref.3. Source: BHF-UCL dorsal/ventral axis specification Inferred from Biological aspect of Ancestor. Source: RefGenome embryonic camera-type eye formation Inferred from expression pattern PubMed 12921742. Source: AgBase embryonic forelimb morphogenesis Inferred from mutant phenotype Ref.1. Source: AgBase iris morphogenesis Inferred from expression pattern Ref.3. Source: BHF-UCL lens development in camera-type eye Inferred from expression pattern Ref.3. Source: BHF-UCL lung induction Inferred from Biological aspect of Ancestor. Source: RefGenome morphogenesis of an epithelial sheet Inferred from direct assay PubMed 12921742. Source: AgBase negative regulation of glial cell differentiation Inferred from direct assay PubMed 15901663. Source: AgBase negative regulation of neuron differentiation Inferred from direct assay PubMed 15901663. Source: AgBase positive regulation of branching involved in ureteric bud morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of fibroblast growth factor receptor signaling pathway Inferred from mutant phenotype Ref.1. Source: AgBase regulation of canonical Wnt receptor signaling pathway Inferred from direct assay Ref.1. Source: AgBase retina layer formation Inferred from direct assay PubMed 12921742. Source: AgBase
Cellular_component	extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	frizzled-2 binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 385	365	Protein Wnt-2b		PRO_0000392925
Amino acid modifications
	Lipidation	101	1	S-palmitoyl cysteine By similarity
	Lipidation	237	1	O-palmitoyl serine; by PORCN By similarity
	Glycosylation	111	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q98SN7 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: FE3204C08A3E6EF5	FASTA	385	42,953
	10 20 30 40 50 60 MLSPNRLAAS PGIAVAPGRA ECGAFPRTAG AAPRICLPFV LILLALTPRA DSSWWYIGAL 70 80 90 100 110 120 GARVICDNIP GLVNKQRQLC QRYPDIMQSV GEGAKEWIRE CQYQFRHHRW NCSTLDRDHT 130 140 150 160 170 180 VFGRVMLRSS REAAFVYAIS SAGVVYAITR ACSQGELKAC GCDPLKRGRA KDERGEFDWG 190 200 210 220 230 240 GCSDNINYGI RFAKAFVDAK EKKVKDARAL MNLHNNRCGR MAVKRFLKLE CKCHGVSGSC 250 260 270 280 290 300 TLRTCWLAMS DFRKTGDYLQ KKYNGAIQVI MNQDGTGFTV ANKNFRKPTK TDLVYFENSP 310 320 330 340 350 360 DYCVMDKSAG SLGTAGRVCN KVSRGTDGCE VMCCGRGYDT TRVTRVTKCE CKFHWCCAVR 370 380 CKECEDTVDV HTCKAPKRAE WLDQT

References

[1]	"WNT signals control FGF-dependent limb initiation and AER induction in the chick embryo." Kawakami Y., Capdevila J., Buscher D., Itoh T., Esteban C.R., Belmonte J.C. Cell 104:891-900(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
[2]	"Analysis of chicken Wnt-13 expression demonstrates coincidence with cell division in the developing eye and is consistent with a role in induction." Jasoni C., Hendrickson A., Roelink H. Dev. Dyn. 215:215-224(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 71-385, FUNCTION, TISSUE SPECIFICITY. Tissue: Head.
[3]	"Expression patterns of Wnt genes during development of an anterior part of the chicken eye." Fokina V.M., Frolova E.I. Dev. Dyn. 235:496-505(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF346628 mRNA. Translation: AAK38108.1. AF182403 mRNA. Translation: AAD55446.1.
IPI	IPI00588366.
RefSeq	NP_989667.1. NM_204336.1.
UniGene	Gga.50310.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000039420.
Proteomic databases
PaxDb	Q98SN7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	374243.
KEGG	gga:374243.
Organism-specific databases
CTD	7482.
Phylogenomic databases
eggNOG	NOG284879.
HOGENOM	HOG000039529.
HOVERGEN	HBG001595.
InParanoid	Q9PUI3.
KO	K00182.
Family and domain databases
InterPro	IPR005817. Wnt. IPR009140. Wnt2. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit. PTHR12027:SF21. PTHR12027:SF21. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01842. WNT2PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20813739.

Entry information

Entry name

WNT2B_CHICK

Accession

Primary (citable) accession number: Q98SN7
Secondary accession number(s): Q9PUI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	June 1, 2001
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program