Reviewed, UniProtKB/Swiss-Prot Q98SM3 (EZH2A_XENLA)

Names and origin

Protein names

Recommended name:
    Histone-lysine N-methyltransferase EZH2
    EC=2.1.1.43
Alternative name(s):
    Enhancer of zeste homolog 2-A

Gene names

Name:	ezh2-A
Synonyms:	xez

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	748 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Polycomb group (PcG) protein By similarity. Catalytic subunit of the prc2/eed-ezh2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene By similarity. May repress transcription of the egr2 and en2 genes. Ref.1
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
Subunit structure	Component of the prc2/eed-ezh2 complex By similarity.
Subcellular location	Nucleus By similarity.
Developmental stage	Does not appear to be maternally expressed. Zygotic expression begins at late blastula, increases at mid and late gastrula, and peaks in the early neurula. Expression is restricted to the anterior nervous system. Ref.1
Induction	Induced by inhibition of BMP signaling. Ref.1
Sequence similarities	Belongs to the histone-lysine methyltransferase family. EZ subfamily. Contains 1 SET domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Repressor Transferase
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 748	748	Histone-lysine N-methyltransferase EZH2		PRO_0000345428
Regions
	Domain	613 – 733	121	SET
	Compositional bias	183 – 211	29	Asp-rich
	Compositional bias	525 – 590	66	Cys-rich
Experimental info
	Sequence conflict	25	1	A → R in AAH84193. Ref.2
	Sequence conflict	31	1	K → R in AAH84193. Ref.2
	Sequence conflict	290	1	P → R in AAH84193. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q98SM3-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 8618F615B348C46C	FASTA	748	85,383
	10 20 30 40 50 60 MGQTGKKSEK GPVCWRKRVK SEYMALRQLK KFRRADEVKS MFNTNRQKIM ERTEILNQEW 70 80 90 100 110 120 KQRRIQPVHI MTTVSSLRGT RECSVTSDLD FPKQVIPLKT LTAVASVPIM YSWSPLQQNF 130 140 150 160 170 180 MVEDETVLHN IPYMGDEVLD QDGTFIEELI KNYDGKVHGD RECGFINDEI FVELVNALAQ 190 200 210 220 230 240 YSDYEDDEDG DDNQDDEQDD TAKDQDDNME DKETQPLRKF PSDKIFEAIS SMFPDKGTSE 250 260 270 280 290 300 ELKEKYKELT EQQLPGALPP ECTPNIDGPN AKSVQREQSL HSFHTLFCRP CFKYDCFLHP 310 320 330 340 350 360 FHATPNTYKR KNNEAANDGK PCGPHCYQLL EGAREFAAAL TAERIKTPPK RPSGRRRGRL 370 380 390 400 410 420 PNNTSRPSTP TVNVSEAKDT DSDREAGTET GGESNDKEEE EKKDETSSSS EANSRCQTPI 430 440 450 460 470 480 KMKPNIEPPE NVEWSGAEAS LFRVLIGTYY DNFCAIARLI GTKTCRQVYE FRVKESSIIS 490 500 510 520 530 540 PVIAEDVDTP PRKKKRKHRL WAAHCRKIQL KKDGSSNHVY NYQPCDHPRQ PCDSSCPCVI 550 560 570 580 590 600 AQNFCEKFCQ CSSECQNRFP GCRCKAQCNT KQCPCYLAVR ECDPDLCLTC GAADHWDSKN 610 620 630 640 650 660 VSCKNCSIQR GSKKHLLLAP SDVAGWGIFI NDTVQKNEFI SEYCGEIISQ DEADRRGKVY 670 680 690 700 710 720 DKYMCSFLFN LNNDFVVDAT RKGNKIRFAN HSVNPNCYAK VMMVNGDHRI GIFAKRAIQT 730 740 GEELFFDYRY SQADALKYVG IEREMEIP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Xenopus Enhancer of Zeste (XEZ); an anteriorly restricted polycomb gene with a role in neural patterning." Barnett M.W., Seville R.A., Nijjar S., Old R.W., Jones E.A. Mech. Dev. 102:157-167(2001) [PubMed: 11287189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, INDUCTION.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF351126 mRNA. Translation: AAK30208.1. BC084193 mRNA. Translation: AAH84193.1.
RefSeq	NP_001083886.1.
UniGene	Xl.19136
3D structure databases
HSSP	HSSP built from PDB template 1ML9 based on UniProtKB Q8X225.
SMR	Q98SM3. Positions 40-68, 555-734.
ModBase	Search...
Genome annotation databases
GeneID	399174.
KEGG	xla:399174.
Organism-specific databases
CTD	399174.
Xenbase	XB-FEAT-956214. ezh2.
Phylogenomic databases
HOVERGEN	Q98SM3.
Family and domain databases
InterPro	IPR001005. SANT_DNA-bd. IPR001214. SET_dom. [Graphical view]
Pfam	PF00856. SET. 1 hit. [Graphical view]
SMART	SM00717. SANT. 2 hits. SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS50280. SET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EZH2A_XENLA

Accession

Primary (citable) accession number: Q98SM3
Secondary accession number(s): Q5XH80

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	June 1, 2001
Last modified:	February 9, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project