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Q98RB6 (SYL_MYCPU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:MYPU_0930
OrganismMycoplasma pulmonis (strain UAB CTIP) [Complete proteome] [HAMAP]
Taxonomic identifier272635 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length807 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 807807Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152051

Regions

Motif38 – 4912"HIGH" region HAMAP-Rule MF_00049
Motif579 – 5835"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5821ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q98RB6 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: C888CC0524A9A3A1

FASTA80793,884
        10         20         30         40         50         60 
MYNHKEIEKK WQKIWDQSKA FKTGNKSDKK YYVLDMFPYP SGSGLHVGHP EGYTATDIIA 

        70         80         90        100        110        120 
RFKRLKGFDV LHPMGWDAFG LPAEQYAIST GNNPNEFTQK NIATFKKQIK SLGLSYDFDK 

       130        140        150        160        170        180 
EVNTTDPKFY EQTQWIFKEL YKKGLAVLAD IDVNWCEELG TVLANEEVLI DKDGNKVSER 

       190        200        210        220        230        240 
GSFPVVKKKM RQWVLKITNY ADKLLEGLED LDWENSLKLL QKNWIGKSTG TKVKFALELL 

       250        260        270        280        290        300 
DESIEVFTTR IETIFGATFL TISPEHPLVE KIVTSENKEK VKDFIKEFEK LDDRQKADKN 

       310        320        330        340        350        360 
EKNGIFTGSY AINPFNQKKI PIWIGDFVLL SYGTGAIMSV PAHDKRDYEF ANKYGLEIKQ 

       370        380        390        400        410        420 
VIVSKENVEL PYLESGHLIN SSEFNGLSSK EAIEKLNQYV EKNNLGQVET FYKLRDWIFS 

       430        440        450        460        470        480 
RQRYWGEPFP VAFDDENNVY LIDGLVELPF MENIKPSKNG QSPLFNNKKW LYFEKDGKKL 

       490        500        510        520        530        540 
TRETNTMPQW AGSNWYYLAY ILKNADGSYE KLDSEEAKKR FKKWLPVDLY IGGQEHAVLH 

       550        560        570        580        590        600 
LLYSRFWHRF LYDIGVVPTK EPFQKVVNQG MILGTDGQKM SKSRGNIINP SEIVDELGAD 

       610        620        630        640        650        660 
TLRVYEMFMG PLTDDKDWQV ESIKGIRKWL ERVYRLFEMF FDGQKTIEKS NEDHLILSQY 

       670        680        690        700        710        720 
NKLIKEIENE VELLKFNTAI SKLMVFVNLL YKVEKIPSWE ILKNFALILS LFAPHIAEEL 

       730        740        750        760        770        780 
LEKMNQKQVK DQIWPTYDPT YLESNLTKYV IQINGKVRAI VDFELDKTQE EVLAKAMQIE 

       790        800 
KIKTLLENKN IIKVIFVANK VLNLIVK 

« Hide

References

[1]"The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis."
Chambaud I., Heilig R., Ferris S., Barbe V., Samson D., Galisson F., Moszer I., Dybvig K., Wroblewski H., Viari A., Rocha E.P.C., Blanchard A.
Nucleic Acids Res. 29:2145-2153(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: UAB CTIP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL445563 Genomic DNA. Translation: CAC13266.1.
PIRE90523.
RefSeqNP_325924.1. NC_002771.1.

3D structure databases

ProteinModelPortalQ98RB6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272635.MYPU_0930.

Proteomic databases

PRIDEQ98RB6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC13266; CAC13266; CAC13266.
GeneID911597.
KEGGmpu:MYPU_0930.
PATRIC20023052. VBIMycPul29704_0092.

Organism-specific databases

GenoListMYPU_0930.
CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_MYCPU
AccessionPrimary (citable) accession number: Q98RB6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: October 1, 2001
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries