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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Mycoplasma pulmonis (strain UAB CTIP)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341S-adenosyl-L-methionineUniRule annotation
Binding sitei59 – 591S-adenosyl-L-methionineUniRule annotation
Binding sitei86 – 861S-adenosyl-L-methionineUniRule annotation
Active sitei107 – 1071By similarity
Binding sitei107 – 1071S-adenosyl-L-methionineUniRule annotation
Binding sitei111 – 1111SubstrateUniRule annotation
Binding sitei143 – 1431SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:MYPU_1660
OrganismiMycoplasma pulmonis (strain UAB CTIP)
Taxonomic identifieri272635 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000528 Componenti: Chromosome

Organism-specific databases

GenoListiMYPU_1660.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 203203tRNA (guanine-N(7)-)-methyltransferasePRO_0000171360Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272635.MYPU_1660.

Structurei

3D structure databases

ProteinModelPortaliQ98R44.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni181 – 1844Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiDNVMTEY.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q98R44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRNNKNAK LELESCENVI KNFPFKVEKN STLEIGMGKG QMLIELAQKN
60 70 80 90 100
PQKIFVGIEK YPTVALIAAK KAKKLELNNF FIIVDDVENA LDFFEGTFDL
110 120 130 140 150
IWLTFSDPWP KKRHYKRRLT YEKFLKIYSK ILSENGLIKL KTDNDDFFQW
160 170 180 190 200
TLESLEKNGL NIINKTSDLE KSEFAKDNVK TSYEQKFVSL GKNINFVEFS

FKK
Length:203
Mass (Da):23,782
Last modified:October 1, 2001 - v1
Checksum:i2A28826B06FBBE29
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445563 Genomic DNA. Translation: CAC13339.1.
PIRiF90532.
RefSeqiNP_325997.1. NC_002771.1.
WP_010924970.1. NC_002771.1.

Genome annotation databases

KEGGimpu:MYPU_1660.
PATRICi20023210. VBIMycPul29704_0166.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445563 Genomic DNA. Translation: CAC13339.1.
PIRiF90532.
RefSeqiNP_325997.1. NC_002771.1.
WP_010924970.1. NC_002771.1.

3D structure databases

ProteinModelPortaliQ98R44.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272635.MYPU_1660.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGimpu:MYPU_1660.
PATRICi20023210. VBIMycPul29704_0166.

Organism-specific databases

GenoListiMYPU_1660.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiDNVMTEY.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UAB CTIP.

Entry informationi

Entry nameiTRMB_MYCPU
AccessioniPrimary (citable) accession number: Q98R44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: October 1, 2001
Last modified: April 1, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.