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Q98R44 (TRMB_MYCPU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:MYPU_1660
OrganismMycoplasma pulmonis (strain UAB CTIP) [Complete proteome] [HAMAP]
Taxonomic identifier272635 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length203 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 203203tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171360

Regions

Region181 – 1844Substrate binding By similarity

Sites

Active site1071 By similarity
Binding site341S-adenosyl-L-methionine By similarity
Binding site591S-adenosyl-L-methionine By similarity
Binding site861S-adenosyl-L-methionine By similarity
Binding site1071S-adenosyl-L-methionine By similarity
Binding site1111Substrate By similarity
Binding site1431Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q98R44 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: 2A28826B06FBBE29

FASTA20323,782
        10         20         30         40         50         60 
MRLRNNKNAK LELESCENVI KNFPFKVEKN STLEIGMGKG QMLIELAQKN PQKIFVGIEK 

        70         80         90        100        110        120 
YPTVALIAAK KAKKLELNNF FIIVDDVENA LDFFEGTFDL IWLTFSDPWP KKRHYKRRLT 

       130        140        150        160        170        180 
YEKFLKIYSK ILSENGLIKL KTDNDDFFQW TLESLEKNGL NIINKTSDLE KSEFAKDNVK 

       190        200 
TSYEQKFVSL GKNINFVEFS FKK 

« Hide

References

[1]"The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis."
Chambaud I., Heilig R., Ferris S., Barbe V., Samson D., Galisson F., Moszer I., Dybvig K., Wroblewski H., Viari A., Rocha E.P.C., Blanchard A.
Nucleic Acids Res. 29:2145-2153(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: UAB CTIP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL445563 Genomic DNA. Translation: CAC13339.1.
PIRF90532.
RefSeqNP_325997.1. NC_002771.1.

3D structure databases

ProteinModelPortalQ98R44.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272635.MYPU_1660.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC13339; CAC13339; CAC13339.
GeneID911277.
KEGGmpu:MYPU_1660.
PATRIC20023210. VBIMycPul29704_0166.

Organism-specific databases

GenoListMYPU_1660.
CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMADNVMTEY.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_MYCPU
AccessionPrimary (citable) accession number: Q98R44
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: October 1, 2001
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways