Q98QL4 (SYFB_MYCPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase beta chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase beta chain
Short name=PheRS

Gene names

Name:	pheT
Ordered Locus Names:	MYPU_3470

Organism

Mycoplasma pulmonis (strain UAB CTIP) [Complete proteome] [HAMAP]

Taxonomic identifier

272635 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	718 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00283
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00283
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00283.
Sequence similarities	Belongs to the phenylalanyl-tRNA synthetase beta chain family. Type 1 subfamily. Contains 1 B5 domain. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 718	718	Phenylalanine--tRNA ligase beta chain HAMAP MF_00283		PRO_0000232813
Regions
	Domain	40 – 153	114	tRNA-binding
	Domain	387 – 462	76	B5
Sites
	Metal binding	440	1	Magnesium By similarity
	Metal binding	446	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	449	1	Magnesium By similarity
	Metal binding	450	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q98QL4 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 21443E7C28EBE867	FASTA	718	83,815
	10 20 30 40 50 60 MLFSINKLRD LANLDKQITN DQIIDAINKI GFEVESVKNF LNVSKIKFGL IKKVYKNPNA 70 80 90 100 110 120 SNLNVCEIEF EDKMRIIQTT AQNVEQNKYI VAFVENSTFG KIKIEAKEIK GTFSQGMLCS 130 140 150 160 170 180 LEELGFDKSL IRDEFQDKIF LLDKADLKQD PIDFFDLDDQ IVDVSILSNR SDANGYFIMA 190 200 210 220 230 240 LELSAFFETK TNFEIKNEIF KIHPNLKVDE TNTLDFVLIE PQKELKKISL KDQILIMKSN 250 260 270 280 290 300 IKTFNDLVDL TNLNLIMNAM PTHVYDKDKI SNEISVKKVS QEVSILGNKK ISLDNNLVVC 310 320 330 340 350 360 SKEKPISIAG VIGIADFGSD QNTKNFVFEI GRFKTLDIQK SIKSVKLDTQ SSKLSSKKIS 370 380 390 400 410 420 SGTFKMALNF LTSYFDSQVL VSKNLKDKKE SFNFVWEDLN KFLNTDVKKM PHFNRVLKSL 430 440 450 460 470 480 NILGFEFKDN KVYLPTYRHD IEHVQDILEE FLRFYGYENL VFEAPNIKNF ETKNYEEYLN 490 500 510 520 530 540 FIPYLNYSET RTYSLVSKDK NIFNPFNFKE NINLETFHSK EREQIRNSMI LSMEEVLQYN 550 560 570 580 590 600 LKRKVENVNI FEIGIINNSQ RSLALLSNQK SFNRFKEDVE SILKDKLYSF EKGGFDFSHP 610 620 630 640 650 660 LESIIIKDND QLIGYIIRAN AKDFEEDFVY AEILIDKLRN KKIENKSLNY LPLKTLDINF 670 680 690 700 710 EVPSENGLFE VYKTLEKNEK IYSYKLIDHY QKNQISIYTI RIWAFEENIE ELKTLFNL

References

[1]

"The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis." Chambaud I., Heilig R., Ferris S., Barbe V., Samson D., Galisson F., Moszer I., Dybvig K., Wroblewski H., Viari A., Rocha E.P.C., Blanchard A. Nucleic Acids Res. 29:2145-2153(2001) [PubMed: 11353084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UAB CTIP.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL445564 Genomic DNA. Translation: CAC13520.1.
PIR	C90555.
RefSeq	NP_326178.1. NC_002771.1.
3D structure databases
ProteinModelPortal	Q98QL4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	911393.
GenomeReviews	Gene locus MYPU_3470 in contig AL445566_GR.
KEGG	mpu:MYPU_3470.
PATRIC	20023584. VBIMycPul29704_0348.
Organism-specific databases
GenoList	MYPU_3470.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG565848.
OMA	LRGHISE.
PhylomeDB	Q98QL4.
ProtClustDB	PRK00629.
Enzyme and pathway databases
BioCyc	MPUL272635:MYPU_3470-MONOMER.
Family and domain databases
HAMAP	MF_00283. Phe_tRNA_synth_beta1. [Tree]
InterPro	IPR005146. B3/B4_tRNA-bd. IPR009061. DNA-bd_dom_put. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004532. Phe-tRNA-synth_IIc_bsu_bac. IPR020825. Phe-tRNA_synthase_B3/B4. IPR002547. tRNA-bd_dom. IPR005147. tRNA_synthase_B5-dom. [Graphical view]
Gene3D	G3DSA:3.50.40.10. B3_4. 1 hit. G3DSA:3.30.56.20. B5. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01890.
Pfam	PF03483. B3_4. 1 hit. PF03484. B5. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
SMART	SM00873. B3_4. 1 hit. SM00874. B5. 1 hit. [Graphical view]
SUPFAM	SSF56037. B3_4. 1 hit. SSF50249. Nucleic_acid_OB. 1 hit. SSF46955. Putativ_DNA_bind. 1 hit.
TIGRFAMs	TIGR00472. PheT_bact. 1 hit.
PROSITE	PS51483. B5. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFB_MYCPU

Accession

Primary (citable) accession number: Q98QL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program