Q98QJ9 (NAGB_MYCPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucosamine-6-phosphate deaminase
EC=3.5.99.6

Alternative name(s):
GlcN6P deaminase
Short name=GNPDA
Glucosamine-6-phosphate isomerase

Gene names

Name:	nagB
Ordered Locus Names:	MYPU_3620

Organism

Mycoplasma pulmonis (strain UAB CTIP) [Complete proteome] [HAMAP]

Taxonomic identifier

272635 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion By similarity. HAMAP MF_01241
Catalytic activity	D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3. HAMAP MF_01241
Pathway	Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 5/5. HAMAP MF_01241
Sequence similarities	Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-acetylglucosamine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	glucosamine-6-phosphate deaminase activity Inferred from electronic annotation. Source: EC hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Glucosamine-6-phosphate deaminase HAMAP MF_01241		PRO_0000160156
Sites
	Active site	68	1	Proton acceptor; for enolization step By similarity
	Active site	137	1	For ring-opening step By similarity
	Active site	139	1	Proton acceptor; for ring-opening step By similarity
	Active site	144	1	For ring-opening step By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q98QJ9 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 409C382461F498CB	FASTA	256	29,281
	10 20 30 40 50 60 MREIYIFKDL QDLHKFSAKQ IIDQIKIKKD STLGFATGKT PLKTYQLLVK DHQENKTSWK 70 80 90 100 110 120 DITSFNLDEF VDIDPSHPES FIKQMKSNLF DHLDINEQKI NIPKSNSSNP DQEALNYENK 130 140 150 160 170 180 IRKNNGIDLQ FISIGVNGHI AYNEPGTPKD SLTHVSNLTK ETILDLIAKN KFSSIDEVPK 190 200 210 220 230 240 KAITMGVKTI LNQCKKIMMV SFGKEKAQVT KQMLEDKPNE NVTASFLQEH PNCIYILDKE 250 AASLLNEETL KKAKWI

References

[1]	"The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis." Chambaud I., Heilig R., Ferris S., Barbe V., Samson D., Galisson F., Moszer I., Dybvig K., Wroblewski H., Viari A., Rocha E.P.C., Blanchard A. Nucleic Acids Res. 29:2145-2153(2001) [PubMed: 11353084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UAB CTIP.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL445564 Genomic DNA. Translation: CAC13535.1.
PIR	B90557.
RefSeq	NP_326193.1. NC_002771.1.
3D structure databases
ProteinModelPortal	Q98QJ9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	911565.
GenomeReviews	Gene locus MYPU_3620 in contig AL445566_GR.
KEGG	mpu:MYPU_3620.
PATRIC	20023632. VBIMycPul29704_0363.
Organism-specific databases
GenoList	MYPU_3620.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG725991.
OMA	AYNEPNT.
PhylomeDB	Q98QJ9.
ProtClustDB	PRK00443.
Enzyme and pathway databases
BioCyc	MPUL272635:MYPU_3620-MONOMER.
Family and domain databases
HAMAP	MF_01241. GlcN6P_deamin. [Tree]
InterPro	IPR006148. Glc/Gal-6P_isomerase. IPR004547. Glucosamine6P_isomerase. IPR018321. Glucosamine6P_isomerase_CS. [Graphical view]
KO	K02564.
PANTHER	PTHR11280. NagB. 1 hit.
Pfam	PF01182. Glucosamine_iso. 1 hit. [Graphical view]
TIGRFAMs	TIGR00502. NagB. 1 hit.
PROSITE	PS01161. GLC_GALNAC_ISOMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGB_MYCPU

Accession

Primary (citable) accession number: Q98QJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program