Reviewed, UniProtKB/Swiss-Prot Q98QH1 (SYK1_MYCPU)

Names and origin

Protein names

Recommended name:
    Lysyl-tRNA synthetase 1
    EC=6.1.1.6
Alternative name(s):
    Lysine--tRNA ligase 1
      Short name=LysRS 1

Gene names

Name:	lysS1
Ordered Locus Names:	MYPU_3900

Organism

Mycoplasma pulmonis [Complete proteome] [HAMAP]

Taxonomic identifier

2107 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP MF_00252
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00252
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Lysyl-tRNA synthetase 1 HAMAP MF_00252		PRO_0000152652
Sites
	Metal binding	400	1	Magnesium 1 By similarity
	Metal binding	407	1	Magnesium 1 By similarity
	Metal binding	407	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q98QH1-1 [UniParc]. Last modified December 15, 2003. Version 2. Checksum: E4672F765B1909BB	FASTA	491	56,912
	10 20 30 40 50 60 MNDNIKLTEQ EIIRREKLKK YESLGIKTFK KFDHEKINIY SNEIVEKYNH FSKDELLEKQ 70 80 90 100 110 120 IKLIIAGRIL TQRGPFIVIQ DTNGKIQLYV DKKELEEQKE TLALLDIGDI IYVKGTLMKT 130 140 150 160 170 180 MKGELTIKVN FFDLLTKGLT PLAEKYHGLV DVEERYRKRY LDLISNPNIK EIFWTRTKVI 190 200 210 220 230 240 SKIRKFFDEQ NFMEVETPML HSILGGANAR PFKTYHNSLS SSFNLRIATE LPLKKLLVGG 250 260 270 280 290 300 IDRVYEIGRI FRNEGIDTTH NPEFTSIEFY QAYSNLEIMM EQTENLIKFI AKELGLSKIK 310 320 330 340 350 360 NHDVEIDLLA DFKKINMVDA VSEATKVDFR NIDLDKAIEV AGRYKVKVEK YFKVGHIINE 370 380 390 400 410 420 LFELLIEKTL IQPTFVTGHP IEISPLAATS EDERFTERAE LFINTKEYAN MFTELNDPLD 430 440 450 460 470 480 QRRRFEAQLE EKESGNEEAS EIDESFLSAL EYGLPPAGGC GIGIDRLVML LTEKESIREV 490 ILFPTLKKKQ V

References

[1]

"The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis." Chambaud I., Heilig R., Ferris S., Barbe V., Samson D., Galisson F., Moszer I., Dybvig K., Wroblewski H., Viari A., Rocha E.P.C., Blanchard A. Nucleic Acids Res. 29:2145-2153(2001) [PubMed: 11353084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UAB CTIP.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL445564 Genomic DNA. Translation: CAC13563.1. Different initiation.
PIR	F90560.
RefSeq	NP_326221.1.
3D structure databases
HSSP	HSSP built from PDB template 1BBW based on UniProtKB P13030.
ModBase	Search...
Genome annotation databases
GeneID	910934.
GenomeReviews	Gene locus MYPU_3900 in contig AL445566_GR.
KEGG	mpu:MYPU_3900.
Organism-specific databases
MypuList	MYPU_3900.
CMR	Search...
Phylogenomic databases
HOGENOM	Q98QH1.
Enzyme and pathway databases
BioCyc	MPUL272635:MYPU_3900-MON.
BRENDA	6.1.1.6. 274806.
Family and domain databases
HAMAP	MF_00252. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002313. Lys-tRNA-synth_II. IPR018149. Lys-tRNA-synth_II_C. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF4. tRNA-synt_lys_2. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
TIGRFAMs	TIGR00499. lysS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK1_MYCPU

Accession

Primary (citable) accession number: Q98QH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	June 16, 2009
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)