Q98QG4 (SYK2_MYCPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysine--tRNA ligase 2
EC=6.1.1.6

Alternative name(s):
Lysyl-tRNA synthetase 2
Short name=LysRS 2

Gene names

Name:	lysS2
Ordered Locus Names:	MYPU_4020

Organism

Mycoplasma pulmonis (strain UAB CTIP) [Complete proteome] [HAMAP]

Taxonomic identifier

272635 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP MF_00252
Cofactor	Binds 3 magnesium ions per subunit By similarity. HAMAP MF_00252
Subunit structure	Homodimer By similarity. HAMAP MF_00252
Subcellular location	Cytoplasm HAMAP MF_00252.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW lysine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Lysine--tRNA ligase 2 HAMAP MF_00252		PRO_0000152653
Sites
	Metal binding	400	1	Magnesium 1 By similarity
	Metal binding	407	1	Magnesium 1 By similarity
	Metal binding	407	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q98QG4 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 41859220F1F81D5E	FASTA	491	57,073
	10 20 30 40 50 60 MNENIKLTEQ EIIRREKLKK YEDLGVETFK KFDHEKINIY SNEINQKYSN LTKDELLEKQ 70 80 90 100 110 120 IKLIVAGRIL TQRGPFIVIQ DTNGKIQLYI DKKELEERKE TLALLDIGDI IYVKGTLMKT 130 140 150 160 170 180 MKGELTIKVN FFDLLTKGLT PLAEKYHGLV DVEERYRKRY LDLISNPNIK EIFWTRTKII 190 200 210 220 230 240 SKIREFFDKQ NFMEVETPML HSILGGASAR PFKTYHNSLS SSFNLRIATE LPLKKLLVGG 250 260 270 280 290 300 IDRVYEIGRI FRNEGIDTTH NPEFTSIEFY QAYSNLEIMM EQTENLIKFI AKELGLSKIK 310 320 330 340 350 360 NHDVEIDLLA DFKKINMVDA VSEATKVDFR NIDLNKAIEI AKSYKIKVEK YFKVGHIINE 370 380 390 400 410 420 LFELLIEKTL IQPTFVMGYP IEVSPLAACS KDKRFTERAE LFINTKEYAN MFTELNDPLD 430 440 450 460 470 480 QRRRFEAQFE EKESGNEEAS EIDEDFLNAL EYGLPPAGGC GIGIDRLVML LTQKESIREV 490 ILFPTLKKKQ L

References

[1]	"The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis." Chambaud I., Heilig R., Ferris S., Barbe V., Samson D., Galisson F., Moszer I., Dybvig K., Wroblewski H., Viari A., Rocha E.P.C., Blanchard A. Nucleic Acids Res. 29:2145-2153(2001) [PubMed: 11353084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UAB CTIP.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL445564 Genomic DNA. Translation: CAC13575.1.
PIR	B99562.
RefSeq	NP_326233.1. NC_002771.1.
3D structure databases
ProteinModelPortal	Q98QG4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	910931.
GenomeReviews	Gene locus MYPU_4020 in contig AL445566_GR.
KEGG	mpu:MYPU_4020.
PATRIC	20023716. VBIMycPul29704_0405.
Organism-specific databases
GenoList	MYPU_4020.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG631383.
OMA	FRIRSHI.
PhylomeDB	Q98QG4.
ProtClustDB	PRK00484.
Enzyme and pathway databases
BioCyc	MPUL272635:MYPU_4020-MONOMER.
Family and domain databases
HAMAP	MF_00252. Lys_tRNA_synth_class2. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR002313. Lys-tRNA-synth_II. IPR018149. Lys-tRNA-synth_II_C. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K04567.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF4. tRNA-synt_lys_2. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00499. LysS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK2_MYCPU

Accession

Primary (citable) accession number: Q98QG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program