Q98Q81 (SYY_MYCPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	MYPU_4850

Organism

Mycoplasma pulmonis (strain UAB CTIP) [Complete proteome] [HAMAP]

Taxonomic identifier

272635 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02006
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02006
Subunit structure	Homodimer By similarity. HAMAP MF_02006
Subcellular location	Cytoplasm By similarity HAMAP MF_02006.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	Tyrosine--tRNA ligase HAMAP MF_02006		PRO_0000234737
Regions
	Domain	349 – 413	65	S4 RNA-binding
	Motif	43 – 52	10	"HIGH" region HAMAP MF_02006
	Motif	227 – 231	5	"KMSKS" region HAMAP MF_02006
Sites
	Binding site	38	1	Tyrosine By similarity
	Binding site	165	1	Tyrosine By similarity
	Binding site	169	1	Tyrosine By similarity
	Binding site	230	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q98Q81 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 945B29A5B79C5144	FASTA	414	47,699
	10 20 30 40 50 60 MSQNKLKTLI EELKKRKVFN NITSEEKVDL ITLDHGIYVG FDPTAISLHL GNYIQMVNLK 70 80 90 100 110 120 RFQNVGFKTI AILGGATSMI GDPSFKDSER KLLSNETILE NKKHIRKQLE NFGFKVIDNL 130 140 150 160 170 180 DFYKDMNILD YLRSVGKFFN VSTMMSRDSV ANRIQSGLSF TEFSYQTLQA YDFKVLCEKE 190 200 210 220 230 240 NVMMQLGGSD QWGNLVSGLD FINKTLSKNL PTIGITMNLL VDSNGNKIGK STGGASLWID 250 260 270 280 290 300 KTLTSPYVLY QYLLNTNDDD AYNLLLQLTF LQLSEIERIK NEHLKNPKLR LMQSRLSFEV 310 320 330 340 350 360 VKDIHGKEEA QRALHISTSL FKEKNSWFNL SLEDLIQLKG SVDFVPFKDD LFLTLVDSKI 370 380 390 400 410 ISSKREFNEF VRDKSLKING QDVTGLDYDL PWENYDNKYL ILKKGKKQYW VIYK

References

[1]	"The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis." Chambaud I., Heilig R., Ferris S., Barbe V., Samson D., Galisson F., Moszer I., Dybvig K., Wroblewski H., Viari A., Rocha E.P.C., Blanchard A. Nucleic Acids Res. 29:2145-2153(2001) [PubMed: 11353084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UAB CTIP.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL445564 Genomic DNA. Translation: CAC13658.1.
PIR	E90572.
RefSeq	NP_326316.1. NC_002771.1.
3D structure databases
ProteinModelPortal	Q98Q81.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	910962.
GenomeReviews	Gene locus MYPU_4850 in contig AL445566_GR.
KEGG	mpu:MYPU_4850.
PATRIC	20023886. VBIMycPul29704_0489.
Organism-specific databases
GenoList	MYPU_4850.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288125.
OMA	QERNILE.
PhylomeDB	Q98Q81.
Enzyme and pathway databases
BioCyc	MPUL272635:MYPU_4850-MONOMER.
Family and domain databases
HAMAP	MF_02006. Tyr_tRNA_synth_type1. [Tree]
InterPro	IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA-bd. IPR002307. Tyr-tRNA-synth. IPR024088. Tyr-tRNA-synth_bac-type. IPR024107. Tyr-tRNA-synth_bac_1. [Graphical view]
Gene3D	G3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. PS50889. S4. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_MYCPU

Accession

Primary (citable) accession number: Q98Q81

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	October 1, 2001
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program