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Protein

Dihydrofolate reductase

Gene

folA

Organism
Mycoplasma pulmonis (strain UAB CTIP)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (folA)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43SubstrateBy similarity1
Binding sitei48SubstrateBy similarity1
Binding sitei73SubstrateBy similarity1
Binding sitei134SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 23NADPBy similarity2
Nucleotide bindingi30 – 35NADPBy similarity6
Nucleotide bindingi59 – 62NADPBy similarity4
Nucleotide bindingi78 – 81NADPBy similarity4
Nucleotide bindingi115 – 120NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:folA
Ordered Locus Names:MYPU_5370
OrganismiMycoplasma pulmonis (strain UAB CTIP)
Taxonomic identifieri272635 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000528 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863971 – 179Dihydrofolate reductaseAdd BLAST179

Interactioni

Protein-protein interaction databases

STRINGi272635.MYPU_5370

Structurei

3D structure databases

ProteinModelPortaliQ98Q32
SMRiQ98Q32
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 179DHFRPROSITE-ProRule annotationAdd BLAST163

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 23Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

eggNOGiENOG41084DH Bacteria
COG0262 LUCA
HOGENOMiHOG000040233
KOiK00287
OMAiEECFVIG
OrthoDBiPOG091H01KY

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.10, 1 hit
InterProiView protein in InterPro
IPR012259 DHFR
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PANTHERiPTHR22778:SF16 PTHR22778:SF16, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q98Q32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMNLIQLLK GKWRFKMIIS IWAMTKEGLI GKDNQMPWHI KEEFQHFRRQ
60 70 80 90 100
TLHKTLIMGK NTFFSLPKVF DKRKMYVLSH EKDFKIDHPD VEVIYDYKDL
110 120 130 140 150
LKEYANNDKK DIYVAGGLYV YQQLIPLSDI LIVSIVKGSY EGNKYLSVDF
160 170
SNFVQYKQPE IYDQFSVYYY KKKDQKNSI
Length:179
Mass (Da):21,491
Last modified:August 29, 2001 - v1
Checksum:i89F35380370C9D06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445565 Genomic DNA Translation: CAC13710.1
PIRiA90579

Genome annotation databases

EnsemblBacteriaiCAC13710; CAC13710; CAC13710
KEGGimpu:MYPU_5370

Similar proteinsi

Entry informationi

Entry nameiDYR_MYCPU
AccessioniPrimary (citable) accession number: Q98Q32
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: August 29, 2001
Last modified: March 28, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health