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Protein

Dihydrofolate reductase

Gene

folA

Organism
Mycoplasma pulmonis (strain UAB CTIP)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431SubstrateBy similarity
Binding sitei48 – 481SubstrateBy similarity
Binding sitei73 – 731SubstrateBy similarity
Binding sitei134 – 1341SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 232NADPBy similarity
Nucleotide bindingi30 – 356NADPBy similarity
Nucleotide bindingi59 – 624NADPBy similarity
Nucleotide bindingi78 – 814NADPBy similarity
Nucleotide bindingi115 – 1206NADPBy similarity

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro

GO - Biological processi

  1. glycine biosynthetic process Source: InterPro
  2. nucleotide biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:folA
Ordered Locus Names:MYPU_5370
OrganismiMycoplasma pulmonis (strain UAB CTIP)
Taxonomic identifieri272635 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000528: Chromosome

Organism-specific databases

GenoListiMYPU_5370.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179179Dihydrofolate reductasePRO_0000186397Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272635.MYPU_5370.

Structurei

3D structure databases

ProteinModelPortaliQ98Q32.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 179163DHFRPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 233Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated
Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0262.
HOGENOMiHOG000040233.
KOiK00287.
OMAiKNKYPYT.
OrthoDBiEOG6KT2V2.

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q98Q32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMNLIQLLK GKWRFKMIIS IWAMTKEGLI GKDNQMPWHI KEEFQHFRRQ
60 70 80 90 100
TLHKTLIMGK NTFFSLPKVF DKRKMYVLSH EKDFKIDHPD VEVIYDYKDL
110 120 130 140 150
LKEYANNDKK DIYVAGGLYV YQQLIPLSDI LIVSIVKGSY EGNKYLSVDF
160 170
SNFVQYKQPE IYDQFSVYYY KKKDQKNSI
Length:179
Mass (Da):21,491
Last modified:August 29, 2001 - v1
Checksum:i89F35380370C9D06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445565 Genomic DNA. Translation: CAC13710.1.
PIRiA90579.
RefSeqiNP_326368.1. NC_002771.1.
WP_010925338.1. NC_002771.1.

Genome annotation databases

EnsemblBacteriaiCAC13710; CAC13710; CAC13710.
GeneIDi911218.
KEGGimpu:MYPU_5370.
PATRICi20024006. VBIMycPul29704_0548.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445565 Genomic DNA. Translation: CAC13710.1.
PIRiA90579.
RefSeqiNP_326368.1. NC_002771.1.
WP_010925338.1. NC_002771.1.

3D structure databases

ProteinModelPortaliQ98Q32.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272635.MYPU_5370.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC13710; CAC13710; CAC13710.
GeneIDi911218.
KEGGimpu:MYPU_5370.
PATRICi20024006. VBIMycPul29704_0548.

Organism-specific databases

GenoListiMYPU_5370.

Phylogenomic databases

eggNOGiCOG0262.
HOGENOMiHOG000040233.
KOiK00287.
OMAiKNKYPYT.
OrthoDBiEOG6KT2V2.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UAB CTIP.

Entry informationi

Entry nameiDYR_MYCPU
AccessioniPrimary (citable) accession number: Q98Q32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: August 29, 2001
Last modified: January 7, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.