Q98PW8 (K6PF_MYCPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase
Short name=Phosphofructokinase
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase

Gene names

Name:	pfkA
Ordered Locus Names:	MYPU_6010

Organism

Mycoplasma pulmonis (strain UAB CTIP) [Complete proteome] [HAMAP]

Taxonomic identifier

272635 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pulmonis

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	6-phosphofructokinase HAMAP-Rule MF_00339		PRO_0000111966
Regions
	Nucleotide binding	22 – 26	5	ATP By similarity
	Nucleotide binding	155 – 159	5	ATP By similarity
	Nucleotide binding	172 – 188	17	ATP By similarity
Sites
	Active site	128	1	Proton acceptor By similarity
	Metal binding	186	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	188	1	Magnesium By similarity
	Binding site	163	1	Substrate By similarity
	Binding site	252	1	Substrate By similarity
	Binding site	255	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q98PW8 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 14AD0F5B3F956EC5	FASTA	326	35,832
	10 20 30 40 50 60 MIKKIAILTS GGDSPGMNNA IRAIIKTARL YDIETYLVYE GFLGLYNGWI KPSEGIDEDS 70 80 90 100 110 120 YINKGGTFIF SARFVEFAQE KYRQVAKENL LKLGIEALVV IGGDGSYKGA QKLHEMGIKT 130 140 150 160 170 180 IALPGTIDND ITSSDFTIGY DTALNTIVEA VDKIRDTASS HKRCIMVEVM GHGASDLALY 190 200 210 220 230 240 SGMATGSEII VSNDYKLSVE EMAKIVKKQF EKPNKRSVII TVSEFVFKDL QQVAKQIEEL 250 260 270 280 290 300 TNITTKAVVL AHIQRGGYPS ARERINATIL GKHAVLRLKQ GQSGIALGLI KNQVAATPIL 310 320 EALAMPQTRK DLLERRTKSY NDINQA

References

[1]

"The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis." Chambaud I., Heilig R., Ferris S., Barbe V., Samson D., Galisson F., Moszer I., Dybvig K., Wroblewski H., Viari A., Rocha E.P.C., Blanchard A. Nucleic Acids Res. 29:2145-2153(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: UAB CTIP.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL445565 Genomic DNA. Translation: CAC13774.1.
PIR	A99587.
RefSeq	NP_326432.1. NC_002771.1.
3D structure databases
ProteinModelPortal	Q98PW8.
ModBase	Search...
Protein-protein interaction databases
STRING	272635.MYPU_6010.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC13774; CAC13774; CAC13774.
GeneID	911150.
KEGG	mpu:MYPU_6010.
PATRIC	20024144. VBIMycPul29704_0612.
Organism-specific databases
GenoList	MYPU_6010.
CMR	Search...
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000248870.
KO	K00850.
OMA	VENGYEG.
ProtClustDB	PRK03202.
Enzyme and pathway databases
UniPathway	UPA00109; UER00182.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase.
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_MYCPU

Accession

Primary (citable) accession number: Q98PW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	October 1, 2001
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program