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Q98PQ2

- SYI_MYCPU

UniProt

Q98PQ2 - SYI_MYCPU

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma pulmonis (strain UAB CTIP)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei551 – 5511Aminoacyl-adenylateUniRule annotation
Binding sitei595 – 5951ATPUniRule annotation
Metal bindingi862 – 8621ZincUniRule annotation
Metal bindingi865 – 8651ZincUniRule annotation
Metal bindingi879 – 8791ZincUniRule annotation
Metal bindingi882 – 8821ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MYPU_6670
OrganismiMycoplasma pulmonis (strain UAB CTIP)
Taxonomic identifieri272635 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000528: Chromosome

Organism-specific databases

GenoListiMYPU_6670.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 888888Isoleucine--tRNA ligasePRO_0000098425Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272635.MYPU_6670.

Structurei

3D structure databases

ProteinModelPortaliQ98PQ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi61 – 7111"HIGH" regionAdd
BLAST
Motifi592 – 5965"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q98PQ2-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MNEKKDYKDT LNMPQTNFEM QAGLTRKEAQ FRQRWLDNKL YHKILAKNKN
60 70 80 90 100
NKQFVVHDGP PYANGSIHIG HALNKILKDI VVRFKSLQGF YSPFVPGWDT
110 120 130 140 150
HGLPIENKML SELKVNHKQI EVVKLRKEAA KYALNQMLIQ KEQFLKLQML
160 170 180 190 200
SDFEEIYLTL DKNFEAKQLK LFKKMFFDGL IYKGLKPVYW SPSSMSALAE
210 220 230 240 250
AEVEYYDHVS PSIYTCFTIT KGNEFVEVDD ELLIWTTTPW TLIANSGVAV
260 270 280 290 300
GLDIEYSKVK FNKKNYIVAS DLLEKVMEIF GVQKYKVVDT FKGKNLLGVE
310 320 330 340 350
YQRPIKTDLF GIVVAGYHVS IDAGTGLVHM APLFGEDDFI IGQENELDQI
360 370 380 390 400
MHINDDGSIN SEGDEFQGLF YSSANIKIKE FLEKNDKVMF FEYFTHSYPH
410 420 430 440 450
DWRTKKPIIF RGTPQWFVSI DKIKPAILKE IEKIEGRPSW AVKRLATMIE
460 470 480 490 500
NRKTWTISRQ RSWGVPIPIF YNEKNEIVND EKVFDHVIEL VEKYGSDVWF
510 520 530 540 550
EKSVDELLPS EYKNKNWTKE TNIMDVWFDS GSTSIGVEIE GVSVPFDLYL
560 570 580 590 600
EGIDQYRGWF NSSIINSVAY WGQSPYRLLL SHGFVLDGKG KKMSKQLGNV
610 620 630 640 650
VDPQEIIQKY GADILRLWVA NCEYAHDVSV SESIIKQTVE NYRKIRNTIK
660 670 680 690 700
FLLGNLQDYD HSKFNLKLEG IHELINERLK KVKFDILQAY NDYDFNDVIK
710 720 730 740 750
TLTNFLTDLS SFYLSISKDS LYADKINSKE RRMIQYNMYN ILEATLVVIA
760 770 780 790 800
PIMPTTAEDA YDNFNKQDKQ ESVHLEKMFE ATIADDKLEK TWKEFFDLKD
810 820 830 840 850
EVYKEIEVEI ANKKIKRTND AHVTINTKSN FLMSLDLKKL LMIGKISFGS
860 870 880
SLRVETFDSH KCPRCWNHIE KTEVVEDLCQ RCYQTINS
Length:888
Mass (Da):103,303
Last modified:October 1, 2001 - v1
Checksum:iCCECB4F79293A390
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL445565 Genomic DNA. Translation: CAC13840.1.
PIRiC90595.
RefSeqiNP_326498.1. NC_002771.1.
WP_010925468.1. NC_002771.1.

Genome annotation databases

EnsemblBacteriaiCAC13840; CAC13840; CAC13840.
GeneIDi911089.
KEGGimpu:MYPU_6670.
PATRICi20024280. VBIMycPul29704_0679.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL445565 Genomic DNA. Translation: CAC13840.1 .
PIRi C90595.
RefSeqi NP_326498.1. NC_002771.1.
WP_010925468.1. NC_002771.1.

3D structure databases

ProteinModelPortali Q98PQ2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272635.MYPU_6670.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC13840 ; CAC13840 ; CAC13840 .
GeneIDi 911089.
KEGGi mpu:MYPU_6670.
PATRICi 20024280. VBIMycPul29704_0679.

Organism-specific databases

GenoListi MYPU_6670.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UAB CTIP.

Entry informationi

Entry nameiSYI_MYCPU
AccessioniPrimary (citable) accession number: Q98PQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2001
Last modified: October 29, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3