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Q98PQ2

- SYI_MYCPU

UniProt

Q98PQ2 - SYI_MYCPU

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Protein

Isoleucine--tRNA ligase

Gene
ileS, MYPU_6670
Organism
Mycoplasma pulmonis (strain UAB CTIP)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei551 – 5511Aminoacyl-adenylate By similarity
Binding sitei595 – 5951ATP By similarity
Metal bindingi862 – 8621Zinc By similarity
Metal bindingi865 – 8651Zinc By similarity
Metal bindingi879 – 8791Zinc By similarity
Metal bindingi882 – 8821Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:MYPU_6670
OrganismiMycoplasma pulmonis (strain UAB CTIP)
Taxonomic identifieri272635 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000528: Chromosome

Organism-specific databases

GenoListiMYPU_6670.

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 888888Isoleucine--tRNA ligaseUniRule annotationPRO_0000098425Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272635.MYPU_6670.

Structurei

3D structure databases

ProteinModelPortaliQ98PQ2.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi61 – 7111"HIGH" regionUniRule annotationAdd
BLAST
Motifi592 – 5965"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q98PQ2-1 [UniParc]FASTAAdd to Basket

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MNEKKDYKDT LNMPQTNFEM QAGLTRKEAQ FRQRWLDNKL YHKILAKNKN    50
NKQFVVHDGP PYANGSIHIG HALNKILKDI VVRFKSLQGF YSPFVPGWDT 100
HGLPIENKML SELKVNHKQI EVVKLRKEAA KYALNQMLIQ KEQFLKLQML 150
SDFEEIYLTL DKNFEAKQLK LFKKMFFDGL IYKGLKPVYW SPSSMSALAE 200
AEVEYYDHVS PSIYTCFTIT KGNEFVEVDD ELLIWTTTPW TLIANSGVAV 250
GLDIEYSKVK FNKKNYIVAS DLLEKVMEIF GVQKYKVVDT FKGKNLLGVE 300
YQRPIKTDLF GIVVAGYHVS IDAGTGLVHM APLFGEDDFI IGQENELDQI 350
MHINDDGSIN SEGDEFQGLF YSSANIKIKE FLEKNDKVMF FEYFTHSYPH 400
DWRTKKPIIF RGTPQWFVSI DKIKPAILKE IEKIEGRPSW AVKRLATMIE 450
NRKTWTISRQ RSWGVPIPIF YNEKNEIVND EKVFDHVIEL VEKYGSDVWF 500
EKSVDELLPS EYKNKNWTKE TNIMDVWFDS GSTSIGVEIE GVSVPFDLYL 550
EGIDQYRGWF NSSIINSVAY WGQSPYRLLL SHGFVLDGKG KKMSKQLGNV 600
VDPQEIIQKY GADILRLWVA NCEYAHDVSV SESIIKQTVE NYRKIRNTIK 650
FLLGNLQDYD HSKFNLKLEG IHELINERLK KVKFDILQAY NDYDFNDVIK 700
TLTNFLTDLS SFYLSISKDS LYADKINSKE RRMIQYNMYN ILEATLVVIA 750
PIMPTTAEDA YDNFNKQDKQ ESVHLEKMFE ATIADDKLEK TWKEFFDLKD 800
EVYKEIEVEI ANKKIKRTND AHVTINTKSN FLMSLDLKKL LMIGKISFGS 850
SLRVETFDSH KCPRCWNHIE KTEVVEDLCQ RCYQTINS 888
Length:888
Mass (Da):103,303
Last modified:October 1, 2001 - v1
Checksum:iCCECB4F79293A390
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL445565 Genomic DNA. Translation: CAC13840.1.
PIRiC90595.
RefSeqiNP_326498.1. NC_002771.1.
WP_010925468.1. NC_002771.1.

Genome annotation databases

EnsemblBacteriaiCAC13840; CAC13840; CAC13840.
GeneIDi911089.
KEGGimpu:MYPU_6670.
PATRICi20024280. VBIMycPul29704_0679.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL445565 Genomic DNA. Translation: CAC13840.1 .
PIRi C90595.
RefSeqi NP_326498.1. NC_002771.1.
WP_010925468.1. NC_002771.1.

3D structure databases

ProteinModelPortali Q98PQ2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272635.MYPU_6670.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC13840 ; CAC13840 ; CAC13840 .
GeneIDi 911089.
KEGGi mpu:MYPU_6670.
PATRICi 20024280. VBIMycPul29704_0679.

Organism-specific databases

GenoListi MYPU_6670.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: UAB CTIP.

Entry informationi

Entry nameiSYI_MYCPU
AccessioniPrimary (citable) accession number: Q98PQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2001
Last modified: September 3, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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