Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q98PQ2

- SYI_MYCPU

UniProt

Q98PQ2 - SYI_MYCPU

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycoplasma pulmonis (strain UAB CTIP)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Oct 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei551 – 5511Aminoacyl-adenylateUniRule annotation
    Binding sitei595 – 5951ATPUniRule annotation
    Metal bindingi862 – 8621ZincUniRule annotation
    Metal bindingi865 – 8651ZincUniRule annotation
    Metal bindingi879 – 8791ZincUniRule annotation
    Metal bindingi882 – 8821ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:MYPU_6670
    OrganismiMycoplasma pulmonis (strain UAB CTIP)
    Taxonomic identifieri272635 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000528: Chromosome

    Organism-specific databases

    GenoListiMYPU_6670.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 888888Isoleucine--tRNA ligasePRO_0000098425Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272635.MYPU_6670.

    Structurei

    3D structure databases

    ProteinModelPortaliQ98PQ2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi61 – 7111"HIGH" regionAdd
    BLAST
    Motifi592 – 5965"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q98PQ2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNEKKDYKDT LNMPQTNFEM QAGLTRKEAQ FRQRWLDNKL YHKILAKNKN    50
    NKQFVVHDGP PYANGSIHIG HALNKILKDI VVRFKSLQGF YSPFVPGWDT 100
    HGLPIENKML SELKVNHKQI EVVKLRKEAA KYALNQMLIQ KEQFLKLQML 150
    SDFEEIYLTL DKNFEAKQLK LFKKMFFDGL IYKGLKPVYW SPSSMSALAE 200
    AEVEYYDHVS PSIYTCFTIT KGNEFVEVDD ELLIWTTTPW TLIANSGVAV 250
    GLDIEYSKVK FNKKNYIVAS DLLEKVMEIF GVQKYKVVDT FKGKNLLGVE 300
    YQRPIKTDLF GIVVAGYHVS IDAGTGLVHM APLFGEDDFI IGQENELDQI 350
    MHINDDGSIN SEGDEFQGLF YSSANIKIKE FLEKNDKVMF FEYFTHSYPH 400
    DWRTKKPIIF RGTPQWFVSI DKIKPAILKE IEKIEGRPSW AVKRLATMIE 450
    NRKTWTISRQ RSWGVPIPIF YNEKNEIVND EKVFDHVIEL VEKYGSDVWF 500
    EKSVDELLPS EYKNKNWTKE TNIMDVWFDS GSTSIGVEIE GVSVPFDLYL 550
    EGIDQYRGWF NSSIINSVAY WGQSPYRLLL SHGFVLDGKG KKMSKQLGNV 600
    VDPQEIIQKY GADILRLWVA NCEYAHDVSV SESIIKQTVE NYRKIRNTIK 650
    FLLGNLQDYD HSKFNLKLEG IHELINERLK KVKFDILQAY NDYDFNDVIK 700
    TLTNFLTDLS SFYLSISKDS LYADKINSKE RRMIQYNMYN ILEATLVVIA 750
    PIMPTTAEDA YDNFNKQDKQ ESVHLEKMFE ATIADDKLEK TWKEFFDLKD 800
    EVYKEIEVEI ANKKIKRTND AHVTINTKSN FLMSLDLKKL LMIGKISFGS 850
    SLRVETFDSH KCPRCWNHIE KTEVVEDLCQ RCYQTINS 888
    Length:888
    Mass (Da):103,303
    Last modified:October 1, 2001 - v1
    Checksum:iCCECB4F79293A390
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL445565 Genomic DNA. Translation: CAC13840.1.
    PIRiC90595.
    RefSeqiNP_326498.1. NC_002771.1.
    WP_010925468.1. NC_002771.1.

    Genome annotation databases

    EnsemblBacteriaiCAC13840; CAC13840; CAC13840.
    GeneIDi911089.
    KEGGimpu:MYPU_6670.
    PATRICi20024280. VBIMycPul29704_0679.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL445565 Genomic DNA. Translation: CAC13840.1 .
    PIRi C90595.
    RefSeqi NP_326498.1. NC_002771.1.
    WP_010925468.1. NC_002771.1.

    3D structure databases

    ProteinModelPortali Q98PQ2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272635.MYPU_6670.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC13840 ; CAC13840 ; CAC13840 .
    GeneIDi 911089.
    KEGGi mpu:MYPU_6670.
    PATRICi 20024280. VBIMycPul29704_0679.

    Organism-specific databases

    GenoListi MYPU_6670.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: UAB CTIP.

    Entry informationi

    Entry nameiSYI_MYCPU
    AccessioniPrimary (citable) accession number: Q98PQ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 1, 2001
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3