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Q98PH7 (SYW_MYCPU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase
EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:MYPU_7450
OrganismMycoplasma pulmonis (strain UAB CTIP) [Complete proteome] [HAMAP]
Taxonomic identifier272635 [NCBI]
Taxonomic lineageBacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma ›

Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 332332Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000136649

Regions

Motif12 – 209"HIGH" region HAMAP-Rule MF_00140
Motif200 – 2045"KMSKS" region HAMAP-Rule MF_00140

Sites

Binding site2031ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q98PH7 [UniParc].

Last modified October 1, 2001. Version 1.
Checksum: C4F6AED7772CF68E

FASTA33237,588
        10         20         30         40         50         60 
MNKKTLVSGI TSTGKLTLGN YIGAIRNFVK LQDEFNMFIF VADLHSLTNE IVPSVLRKNI 

        70         80         90        100        110        120 
KEIAALYLAC GLDPEKTVLF KQSDVYEHGL MQWILLNQTT IGELSRMTQF KDKSSKITHA 

       130        140        150        160        170        180 
NNTESIPSGL LTYPTLMAAD ILLYNPNLVP VGQDQVQHLE LTRKIARKLN NKYNTKFNEP 

       190        200        210        220        230        240 
TTFVPETGAK IMSLTNPTKK MSKSSDDING TIFLLEDPEL AYKKIKKSIT DSENKVYHDP 

       250        260        270        280        290        300 
SKPGVSNLLE IYACLENKSL KEAQEIFKDK NYLELKEGVG NSVKNFLTKL QAKYQENYKR 

       310        320        330 
VDEILNQGKI KAQKVASYNL NNLMKKIGIR DK

« Hide

References

[1]"The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis."
Chambaud I., Heilig R., Ferris S., Barbe V., Samson D., Galisson F., Moszer I., Dybvig K., Wroblewski H., Viari A., Rocha E.P.C., Blanchard A.
Nucleic Acids Res. 29:2145-2153(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: UAB CTIP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL445565 Genomic DNA. Translation: CAC13918.1.
PIRA99605.
RefSeqNP_326576.1. NC_002771.1.

3D structure databases

ProteinModelPortalQ98PH7.
ModBaseSearch...

Protein-protein interaction databases

STRING272635.MYPU_7450.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC13918; CAC13918; CAC13918.
GeneID911011.
KEGGmpu:MYPU_7450.
PATRIC20024450. VBIMycPul29704_0761.

Organism-specific databases

GenoListMYPU_7450.
CMRSearch...

Phylogenomic databases

eggNOGCOG0180.
HOGENOMHOG000059940.
KOK01867.
OMAQWKSKAQ.
ProtClustDBPRK00927.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_MYCPU
AccessionPrimary (citable) accession number: Q98PH7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2001
Last modified: May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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